How much is 40 billion yuan? I believe that most people, like me, are a relatively abstract concept, simply a big number. Suppose you win 5 million prizes in the lottery, and you win 8,000 times. If it is used to buy in vitro diagnostic reagents, how much can you buy?
How much serum separation gel can be bought for 40 billion?
Usually the price of domestic serum separation gel is tens to one or two hundred per kilogram. We calculate at 100 yuan per kilogram, and 40 billion can buy 400 million kilograms of separation gel. Separating glue is generally 25 kg per barrel, and the barrel is about half a meter high. These barrels of separating glue can be connected to 8 million meters, which is a thousand times the height of Mount Everest and 800 times the depth of the Mariana Trench, the deepest part of the earth.
How much can be done if these separating glues are added to the vacuum blood collection tube? Generally, coagulation tubes or anticoagulation tubes with a blood collection volume of 3-5mL add 0.8-1.2g of separation gel. We calculate by adding 1g separation gel per tube. These separation gels can be made into 400 billion blood collection tubes with a tube length of 10 cm. , Then these blood collection tubes can be connected from the earth to the moon back and forth 50 times, and can also go around the earth's equator 1,000 times, and weave a scarf for the earth.
How much heparin can be bought for 40 billion?
If you feel that the separation glue is not intuitive enough, then use 40 billion to buy heparin. The price of this is much higher. We calculate at a price of about 100 yuan per gram. The money can buy 400,000 kilograms of heparin. On average, 1300 pig small intestines can extract 1 kilogram of heparin. This heparin needs to sacrifice 520 million pigs, which is 4.3 of the total population of Japan. It is 1.6 times the total population of the United States and 70% of the entire European population! My goodness, if so many pig small intestines are used to extract heparin, the remaining pork is even more unimaginable.
Of course, we canâ??t really spend 40 billion to buy separation gel or heparin. There are many in vitro diagnostic reagents that are more expensive than heparin, such as chemiluminescence reagent acridinium ester and enzyme preparations, antigen and antibody protein preparations, and the price is even higher than that of heparin. The milligram calculation is far more than that of gold, but usually the amount of these reagents is not much at one time, so the entire supporting products involved are also many. Desheng is a manufacturer engaged in the research and development of blood testing and virus testing related reagents, and welcomes the cooperation of in vitro diagnostic reagent companies.
With the continuous development of social economy and the increasing frequency of population movement, health issues have become a concern for everyone. Generally, the most common test is blood test. Many problems can be detected through blood. Now let's take a look at what role does the chemiluminescence reagent luminol play in blood testing?
Luminol will only emit light after being treated with an oxidant. Generally, a mixed aqueous solution of hydrogen peroxide and a hydroxide base is used as an activator. Under the catalysis of iron compounds, hydrogen peroxide decomposes into oxygen and water:
Potassium ferricyanide is often used as the source of catalyst iron in the laboratory, while the catalyst in forensic medicine happens to be the iron in hemoglobin. Enzymes in many biological systems can also catalyze the decomposition of hydrogen peroxide.
When luminol reacts with hydroxide, a double negative ion (Dianion) is formed, which can be oxidized by oxygen generated by the decomposition of hydrogen peroxide, and the product is an organic peroxide. The peroxide is very unstable and immediately decomposes oxygen to generate excited 3-aminophthalic acid. In the transition from the excited state to the ground state, the released energy exists in the form of photons with a wavelength in the blue part of visible light. It can also be detected when a drop of blood is mixed with 999 drops of water. However, it also reacts with other oxidizing substances, but the displayed color and the length of time for color development are different.
Desheng has many years of luminol production experience, and the production process is stable. Among them, "A Method for Synthesizing Luminol or Isoluminol in One Pot" has won the invention patent certificate of the State Intellectual Property Office. What else can you hesitate? Decisively choose our Desheng, you will have unexpected surprises.
Choice of nasopharyngeal swab:
The main indicators of clinical evaluation of swab performance are the absorption capacity and release capacity of the swab to the target sample at the sampling site. Nylon flocking swabs are made of fine nylon fibers adhered to a plastic rod. Compared with traditional cotton swabs, the adsorption surface is significantly larger, which is conducive to the adhesion and release of pathogenic microorganisms. The ability of nylon flocking swabs and traditional rayon cotton swabs to recover and release known microbial inoculums (spiked samples) confirms that nylon flocking swabs have the ability to release cells, microorganisms, viruses and other microorganisms Significant advantages. Compared with traditional rayon cotton, nylon flocking swabs or foam swabs have a significant advantage in the virus recovery rate in the screening of nasal cavity carrying MRSA virus.
For the choice of virus preservation solution: non-inactivated preservation solution and inactivated preservation solution.
[Product Name] Sodium heparin
[Package Specification] Plastic bottle, 10g/bottle, 20g/bottle, 50g/bottle
[Product Standard] Q/WHDS 003-2016
[Product Performance] White amorphous powder, odorless, soluble in water, easy to absorb moisture, average molecular weight of 15,000, stable at room temperature. The potency of sodium heparin of our company is â?¥150 IU/Mg, and the potency of anhydrous sodium heparin is â?¥ 160 IU/Mg. Other indexes were controlled according to sodium heparin standard.
� [Scope of application]
1. This product is applicable to the collection and anticoagulation of blood samples for clinical biochemical examination and emergency biochemical examination, and also applicable to the collection and anticoagulation of blood samples for some hemorheology projects. Ã?Â
2. The use of sodium heparin as an anticoagulant is recommended for the determination of ion content in blood in clinical tests because it is the least likely to interfere with the determination of other ions.
3. This product is not a drug and cannot be used as an injection. Direct injection into human body and animals is prohibited.
[Warning and Prevention]
It is forbidden to use sodium heparin when it has impurities, abnormal smell, color and expiration date.
When the sodium heparin solution appears turbid, it may be that the heparin solution has been contaminated with bacteria or deteriorated, please do not continue to use.
This product is a biological preparation, safe, non-toxic and harmless.
[Storage conditions]
The product can be sealed and stored in a cool and dry place, away from light and moisture. The aqueous solution of this product can be sealed at 0-4â?? in sterile state, and the longest storage period shall not exceed 7 days. It is recommended that the aqueous solution of this product be prepared and used immediately, and not stored in solution state for a long time.
[Validity] 3 years
Desheng is an established blood test reagent company with 14 years of R&D and production experience. It has a lot of research on blood collection additives. Enterprises or individuals in need of sodium heparin can directly contact 15071057538(Wechat same number) .
[Product Name] Dipotassium EDTA
[Package Specification] Plastic bottle, 500g/bottle
[Performance] White crystal powder, odorless, soluble in water, easy to absorb moisture, molecular weight of 404.6, pH value of 4.8±1 (5% aqueous solution, g/ml), solubility in water 60% (g/ml, 25). Stable at room temperature. The main content of EDTA in our company is 99.0%. See the product test report for other indicators.
[Scope of application]EDTA salt can protect the cellular components of the blood, does not affect the white blood cell count and size, has the least effect on the morphology of the red blood cell , and can inhibit the aggregation of platelets, which is suitable for general hematological test. Except for the isolation and examination of platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor for the determination of calcium, potassium, sodium, iron, alkaline phosphatase, creatine kinase and leucine amino peptidase and PCR test.
International Committee for hematology Standardization (ICSH) recommended dipotassium EDTA as anticoagulant for blood cell count and classification. Compared with tripotassium EDTA, it has lower solubility and slower anticoagulation speed.Â
 [Warning and Prevention]
When dipotassium EDTA has impurities, foreign matters, abnormal smell, color, serious moisture absorption and the expiry date is exceeded, it is forbidden to use.
Do not continue to use EDTA solution when it is turbid.
This product is a chemical reagent with strong corrosiveness. It is forbidden to touch it directly. When splashing on the skin, wash with plenty of water. When splashing into eyes, wash with plenty of water immediately. In case of emergency, it should be sent to hospital for examination and treatment immediately.
[Storage Conditions] The product can be sealed and stored in a cool and dry place, away from light and moisture. The aqueous solution of this product can be sealed and stored in sterile state at room temperature. It is recommended that the aqueous solution of this product should be prepared and used immediately, and not stored in solution state for a long time.
[Validity] 3 years
Desheng is an established blood test reagent company with 14 years of R&D and production experience. It has a lot of research on blood collection additives. Enterprises or individuals in need of Dipotassium EDTA can directly
[Product Name] Sodium Citrate
[Package Specification] 500g/bottle
[Product Performance] White crystal powder, easy to absorb moisture.
[Application] It can be used as flavor agent and stabilizer in food and beverage industry, as anticoagulant, expectorant and diuretic in pharmaceutical industry; In detergent industry, it can replace sodium tripolyphosphate as the additive of non-toxic detergent. It is an excellent chelating/complexing agent in chemistry and has been used in many industrial applications of sodium citrate. It is also used in brewing, injection, photographic drugs electroplating,etc.
[Special storage conditions and methods] sealed and moisture-proof.
[validity] 3 years (subject to the production date).
Desheng is an old brand blood test reagent company with 14 years of R&D and production experience. It has a deep research on blood collection additives. If you need any business friends of irumino, you can contact 15071057533 (wechat with the same number) directly. We are looking forward to your call!
Desheng is an established blood test reagent company with 14 years of R&D and production experience. It has a lot of research on blood collection additives. Enterprises or individuals in need of sodium citrate can
[Product Name] Sodium fluoride
[Package Specification] 500g/bottle
[Performance] White crystal powder, easy to absorb moisture
[Application] This product can be used for iron and steel analysis, photoelectric colorimetry for phosphorus determination, drop analysis for scandium determination, blood test, masking agent and flux.
[Special Storage Conditions and Methods] Sealed and moisture-proof.
[Validity] 3 years (subject to the production date).
Desheng is an established blood test reagent company with 14 years of R&D and production experience. It has a lot of research on blood collection additives. Enterprises or individuals in need of sodium fluoride can directly
[English name] 4-Aminophthalhydrazide
[Chinese name]4-Aminophthalhydrazide / 6-Amino-2,3-dihydro-1,4-phthalazinedione
[CAS#] 3682-14-2
[Molecular weight] 177.16
[Formula] C8H7N3O2
[Storage condition] Room temperature, away from light and moisture
[Appearance] White like powder
[Application] Chemiluminescent reagent, commonly used in chemiluminescent immunoassay, such as metal cations and blood detection.
[Product advantage] Purity: â?¥ 98% (HPLC), good water solubility, stable process, little difference between batches.
Desheng is a chemical reagent company specializing in the research and development of blood test reagents. It has 14 years of research and production experience so far. Luminescent reagent substrate has always been the top priority of the company's research. Although isoluminol is not widely used as luminol, it plays an irreplaceable role in blood test. Enterprises or individuals in need of isoluminol can directly contact 15071057538(Wechat is the same number) . We� look� forward� to� your� calls,� to� join� hands� in� creating� a� better� tomorrow!
[English name] NSP-SA-ADH
[Chinese name] Acridinium hydrazine NSP-SA-ADH
[Molecular weight] 740.85
[Molecular formula] C34H40N6O9S2
[Appearance] Yellow solid or powder
[Storage conditions] refrigerate at 2-8, sealed, dry and keep away from light
[Application] It can be used to mark proteins, antigens, antibodies, nucleic acids (DNA, RNA), etc. The terminal of acridinium hydrazide is suitable to react with the polysaccharide, nucleic acid or protein containing aldehyde group and label it with acridinium.
[Product advantage] Purity: 98% (HPLC), good water solubility, stable process,little difference between batches.
Desheng is a chemical reagent company specializing in the research and production of blood test reagents. It has 14 years of research and production experience so far. The substrate of luminescent reagent has always been the top priority of the company's research. Acridinium ester series has high luminescent efficiency and belongs to direct luminescent. Enterprises or individuals in need of acridinium
[English name] 2',6'-Dimethylcarbonylphenyl 10-Methyl-9-acridinecarboxylate 4'-NHS Ester Triflate
[Chinese name] Acridinium ester ME-DMAE-NHS
[Molecular weight] 632.56
[Molecular formula] C29H23F3N2O9S
[Appearance] Yellow solid or powder
[Storage conditions] Refrigerate at 2-8 â??, sealed, dry and keep away from light
[Purpose] It can be used to mark proteins, antigens, antibodies, nucleic acids (DNA, RNA), etc.
[Product advantage] Purity: â?¥ 98% (HPLC), good water solubility, stable process, little difference between batches.
Desheng is a chemical reagent company specializing in the research and production of blood test reagents. It has 14 years of research and production experience so far. The substrate of luminescent reagent has always been the top priority of the company's research. Acridinium ester series has high luminescent efficiency and belongs to direct luminescent. Enterprises or individuals in need of acridinium ester can
Carbomer 940 is white and loose; it is acidic, hygroscopic and slightly smelly. It is soluble in water, ethanol, and glycerin. The usual concentration is 0.1% - 3.0%. Carbomer 940 contains a large number of carboxyl groups in its molecule, so the aqueous solution should be used after neutralization with alkali to reduce the irritation to the skin and mucous membranes. The neutralizer of Carbo 940 can be sodium hydroxide, potassium hydroxide, potassium bicarbonate, borax, amino acids, polar organic amines such as triethanolamine. Laurylamine and stearylamine can be used as neutralizers in non-polar systems. The neutralized carbomer hydrogel is the most viscous between pH 6-11, such as pH12, the viscosity will decrease, and the presence of strong electrolytes can also reduce the viscosity. The gel is unstable, and it is easy to grow mold and lose viscosity when exposed to the sun. Adding antioxidants can slow down the reaction.
How to use Kabo 940 (acrylic resin) and matters needing attention:
1.pH value: The optimum pH value range of Carbo 940 system is 4-10, higher or lower than this range will lead to changes in system viscosity.
2.Components that are not easily compatible in the formula: protein, PVP resin, polyethylene glycol (PEG), polyethoxylated surfactant will interact with unneutralized Carbo 940, so it needs to be partially neutralized before adding Capo 940. Desheng professionally provides cosmetic raw materials and technical formula support such as Carbo 940.
3.Salt and soluble cations: Carbo 940 is sensitive to salts and cations. When the concentration of soluble ions in the component exceeds 0.1%, the dosage of the component should be controlled. If possible, use acidic, neutral or alkaline materials as the matrix of the main drug, use deionized water, add salt after neutralization to grasp the degree of salt's influence on the system, high-valent ions (Ca, Mg, Fe, AI) will Serious damage to the system should be eliminated. Self-converted pollutants (such as Fe, Cu, etc.) will gradually reduce the viscosity of the system and cause the system to become unstable. Preparation of products in stainless steel or non-metal equipment will avoid this situation. In addition, adding EDTA to chelate metal ions can also achieve good results. In addition to the above two aspects, a suitable model of Carbo 940 (such as Carbo 9401342GE resin) can also be used, which has low sensitivity to soluble salts.
4. Insoluble matter: When insoluble components are present, it is difficult for the Carbomer 940 system to present a clear and transparent gel.
Sodium citrate is an organic compound. It is a sodium salt. Its chemical formula is Na3C6H5O7. The appearance is white to colorless crystals, with the taste of soapy water. It is easily soluble in water and glycerin, but hardly soluble in alcohols and other organic solvents. Deliquescence, weathering in hot air.
In chemical technology, citric acid can be used as a reagent for chemical analysis, as an experimental reagent, chromatographic analysis reagent and biochemical reagent; as a complexing agent and masking agent; and as a buffer solution.
2. For environmental protection
Sodium citrate buffer is used for flue gas desulfurization. China is rich in coal resources and constitutes the main part of energy. However, there has been a lack of effective flue gas desulfurization technology, which has caused serious atmospheric SO2 pollution. To study an effective desulfurization process is indeed a top priority. Citric acid-sodium citrate buffer solution is a desulfurization absorbent with great development value due to its low vapor pressure, non-toxicity, stable chemical properties, and high absorption rate of SO2.
3. Used in cosmetics
Citric acid is a kind of fruit acid. Its main function is to accelerate the renewal of keratin. It is often used in lotions, creams, shampoos, whitening products, anti-aging products, and acne products. The renewal of keratin helps to peel off the melanin of the skin, shrink the pores, and dissolve blackheads.
4. Used in medicine
During the formation of prothrombin activator and the subsequent clotting process, calcium ions must be involved. Citrate ions and calcium ions can form a soluble complex that is difficult to dissociate, thereby reducing the concentration of calcium ions in the blood and hindering blood coagulation. It is often used as an in vitro anticoagulant during blood transfusion or laboratory blood sample anticoagulation.
Desheng specializes in the research and development, production and sales of blood collection tube additives, in vitro diagnostic reagents, biological buffers, and luminescent substrates. In terms of blood collection tube additives, it has formed independent intellectual property rights and professional production research and development capabilities. A variety of additives can be produced. The blood sample anticoagulation series products include heparin sodium, heparin lithium, sodium citrate, EDTA dipotassium, EDTA tripotassium, potassium oxalate, etc.; the blood sample procoagulant series products include blood coagulation powder, blood coagulation The materials used in the pretreatment of blood samples include separating gels, siliconizing agents, etc.
Citrate is mainly used for hemostasis testing and the determination of erythrocyte sedimentation rate. Generally, the INP value of sodium citrate with a concentration of 0.129mol/L is higher than that with a concentration of 0.109mol/L. In addition, for thromboplastin reagents from different sources, the clotting time of the plasma of the same normal person or patient can vary greatly . Therefore, the source of the thromboplastin reagent used in each laboratory should be as constant as possible, and the preparation method should be standardized, otherwise the clotting time will be prolonged or shortened, or the normal value will be irregular. Conditional laboratories can use buffered anticoagulants, which are more suitable for analysis of specimens after cryopreservation. The function of the buffer is to maintain a constant pH value of plasma and prevent the inactivation of volatile factors.
1ã??Anticoagulation mechanism
Citrate is mainly sodium citrate. The principle of anticoagulation is to combine with Ca2+ in the blood to form a chelate, so that Ca2+ loses coagulation function. Calcium is an essential substance in the coagulation process and can promote the formation of thromboplastin (coagulation factor III), thrombin and fibrin. And activate the release response of platelets. Citrate ions in citrate and calcium ions in the blood form a soluble complex calcium citrate that is difficult to dissociate. This complex is easily soluble in water but not easily dissociated, reducing calcium ions in the blood and clotting. The process is inhibited, thereby preventing blood clotting.
2ã??Clinical application
Sodium citrate and blood are used at a ratio of 1:9.
Most coagulation tests can be used for anticoagulation with sodium citrate, which helps stabilize factor V and factor VIII, and has little effect on average platelet volume and other coagulation factors. It can be used for platelet function analysis.
Sodium citrate is less cytotoxic, and it is also one of the components of blood maintenance liquid in blood transfusion.
3ã??Notes
6mg of sodium citrate can be used to anticoagulate 1ml of blood, which is strong alkaline; also because of its weak anticoagulant effect and strong alkaline, it is not suitable for blood tests and biochemical tests. Use 5 to 7% sodium citrate solution for acute blood pressure experiments.
Desheng is a veteran blood testing reagent company with 15 years of R&D and production experience, and has in-depth research on blood collection tube additives.
Luminol, is a yellow crystal or beige powder at room temperature and is a relatively stable chemical reagent. At the same time, luminol is a strong acid, which has a certain irritating effect on eyes, skin and respiratory tract.
It is one of the oldest and most commonly used reagents. It can be oxidized by peroxide under alkaline conditions and emit light at the same time. The redox reaction between luminol and peroxide requires a catalyst, which is generally more Valence metal ions, peroxidases such as iron, horseradish peroxidase, etc. This method is often used to detect the content of peroxides, heavy metals, peroxidase, and the derived free radicals, for toxicant analysis And analysis methods based on peroxidase and glucose oxidase.
Under normal circumstances, the chemiluminescence reaction between luminol and hydrogen peroxide is very rapid in the presence of certain catalysts. The most commonly used catalyst is metal ions. In a large concentration range, the concentration of metal ions is directly proportional to the luminescence intensity, so that chemiluminescence analysis of certain metal ions can be performed. This reaction can be used to analyze organic compounds containing metal ions to achieve Very high sensitivity. The second is to use the inhibitory effect of organic compounds on the chemiluminescence reaction of luminol to determine the organic compounds that have a quenching effect on the chemiluminescence reaction. The third is the indirect determination of inorganic or organic compounds through coupling reactions.
Luminol Luminescence Principle
One is that sodium hypochlorite oxidizes luminol to make it glow;
The second is that hydrogen peroxide reacts with sodium hypochlorite to generate oxygen and oxidize luminol to make it glow:
The first is the equation for the reaction of sodium hypochlorite and hydrogen peroxide: NaClO+ H2O2== NaCl + O2+ H2O
Secondly, when luminol reacts with hydroxide, a double negative ion (Dianion) is formed, which can be oxidized by oxygen generated by the decomposition of hydrogen peroxide, and the product is an organic peroxide. The peroxide is very unstable and immediately decomposes nitrogen (Luminol is not oxidized by organic oxidants such as dimethyl sulfoxide to generate nitrogen, but to generate nitrogen-containing organics) to generate excited 3-aminophthalic acid . In the transition from the excited state to the ground state, the released energy exists in the form of photons with a wavelength in the blue part of visible light.
The method of indirect determination by coupling refers to the combination of one reaction that can produce or consume chemiluminescence reactants with another chemiluminescence reaction, so that indirect chemiluminescence determination of certain substances can be achieved. This method is used when determining the purity of certain substrate enzymes.
The Chinese name of Tris is tris(hydroxymethyl)aminomethane, tromethamine, tromethamine, 2-amino-2-(hydroxymethyl)-1,3-propanediol. It is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in ether and carbon tetrachloride, corrosive to copper and aluminum, and irritating chemicals.
Tris has a high buffer capacity, high solubility in water, and is inert to many enzymatic reactions, making Tris a very satisfactory buffer for many biochemical purposes. It is generally used to stabilize the pH of the reaction system, and has a strong buffering capacity between pH 7.5-9.0.
Advantages of Tris buffer:
1. Because Tris base is more alkaline, you can only use this buffer system to configure a buffer with a wide range of pH ranging from acidic to alkaline;
2. It has little interference to biochemical processes, and does not precipitate with calcium, magnesium ions and heavy metal ions.
Disadvantages of Tris buffer:
1. The pH of the buffer is greatly affected by the concentration of the solution, the buffer is diluted ten times, and the pH change is greater than 0.1;
2. The temperature effect is large, and the temperature change has a great influence on the pH of the buffer. The pH of the buffer at 4�?�°C is 8.4, and the pH at 37�?�°C is 7.4. It must be prepared at the temperature of use, and prepared at room temperature Tris-HCL buffer cannot be used for 0-4;
3. It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed;
4. This buffer has a certain interference effect on some PH electrodes, so use electrodes that are compatible with Tris solution.
Application of Tris buffer:
It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH; uses the Tris-HCL buffer system in the gel to stabilize the pH; it is widely used as a solvent for nucleic acids and proteins; the low ionic strength of the Tris buffer can also be used The formation of intermediate fibers of nematodes; add EDTA to Tris hydrochloric acid buffer to make "TE buffer", which can be used for DNA stabilization and storage; change the pH-adjusting acid solution to acetic acid to obtain "TAE buffer", change "TBE buffer" can be obtained as boric acid. These two solutions are used in nucleic acid electrophoresis experiments.
Acridine ester (NSP-DMAE-NHS), yellow powder, CAS number: 194357-64-7, is an important chemiluminescence reagent, its chemiluminescence quantum yield is higher than luminol, and it is labeled with acridine ester The conditions are mild, the labeling rate is high, and the separation is not affected after labeling. In addition, the chemiluminescence process of acridinium ester has a rapid reaction and low background, which can emit light in the presence of sodium hydroxide and hydrogen peroxide. During the oxidation reaction, the conjugate is decomposed and does not affect the luminescence of free acridine esters; in addition, acridine esters chemiluminescent reagents have good stability and are easy to store.
In addition to its application in immunoassays, acridinium esters can also be used to label oligonucleotide fragment probes for genetic or microbial assays. Acridine ester compounds are very suitable for labeling DNA strands to produce chemiluminescent DNA probes. Modern medical research results show that many diseases such as cancer and genetic diseases are related to DNA mutations, and many infectious diseases are caused by viruses, bacteria or parasites in the environment. The analysis of virus specific sequence DNA is beneficial Control of the epidemic. The detection of DNA sequence and base mutation in its chain is of far-reaching significance in gene screening, early diagnosis and treatment of genetic diseases, and pathogenic gene determination.
In nucleic acid hybridization analysis, the preparation of labeled probes with strong specificity and high sensitivity is the key to successful nucleic acid hybridization analysis. Acridinium ester derivatives can be directly labeled on nucleic acid probes without the need for catalysts and the luminescence quantum yield is not affected. In addition, under certain conditions, the labeled acridinium ester on the unhybridized single-stranded DNA is hydrolyzed and destroyed, and only the double-stranded protected acridinium ester formed by hybridization can produce chemiluminescence, and the entire hybridization process can be monitored without separation.
Desheng Technology has been developing chemiluminescence reagents for 12 years. After successful development, it has been put into the market and has been widely recognized by customers. The acridinium esters developed and produced have 6 different groups of products. In addition to the acridinium esters, chemiluminescent reagents also include luminol and isoluminol. The luminous efficiency of the acridine ester series is relatively high and belongs to direct luminescence.
The raw materials of heparin sodium are diversified and can be extracted from the small intestinal mucosa of pigs, cattle, sheep and cattle lungs. However, due to the emergence of mad cow disease, the application of heparin from cattle has been restricted. Studies have found that different sources of heparin sodium have different chemical structures and biological activities, and their adverse reactions are also different. The adverse reaction of heparin sodium from bovine source to thrombocytopenia is about twice that of heparin sodium from pig source. This has caused requirements and restrictions on the source of raw materials in various countries. It is safer to use heparin sodium from pig source.
At present, foreign manufacturers do not purchase heparin sodium from cattle, goats and sheep, but only purchase heparin sodium from pigs in order to prevent the infection of mad cow disease and scrapie. Heparin sodium mixed with cattle, goat, and sheep sources is regarded as a substandard product. Therefore, distinguishing heparin sodium from different species has been used as an important detection index.
At present, there are many methods for detecting the sources of different species of heparin sodium, such as PCR (polymerase chain reaction), NMR (nuclear magnetic resonance), ESI-MS (electrospray mass spectrometry), but these methods are usually expensive At the same time, the detection steps are cumbersome and complicated. Based on this, it is particularly important to find a simple and quick method to distinguish the sources of heparin sodium from different species. Desheng uses heparinase to enzymatically hydrolyze heparin sodium from different species, combined with anion exchange high-efficiency solution The composition analysis of disaccharides by SAX-HPLC has shown that the method is simple and effective and can be used as a method for detecting heparin sodium from different species.
Desheng is a professional manufacturer of blood collection tube reagents. It has more than ten years of R&D and production, experience, and perfect quality inspection and quality assurance system. The heparin sodium developed and produced is all derived from porcine intestinal mucosa and is mainly used as an additive for blood collection tubes. Heparin sodium has good anticoagulant effect, potency 150IU, high purity, fast delivery, welcome to consult and order!
As a veteran manufacturer of blood collection tube additives, Desheng has a small workshop to today's large factory. In the ups and downs of the world, it can always maintain its progress, which can only mean that it has been keeping up with the pace of this era, working hard to make every product of it, so that all those who have been exposed to their products Both individuals and companies applauded. I think the success of an enterprise is ultimately inseparable from product quality and service.
Since it is an old blood collection tube additive manufacturer, what are their blood collection tube additive products? Serum separation gel, heparin sodium, lithium heparin, dipotassium EDTA, tripotassium EDTA, coagulant, siliconizer, sodium citrate, potassium oxalate, etc. Each product has its characteristics and uses, but who is the one among these products? From the point of view of use, sales and production, the serum separation gel deserves to sit on the throne.
Serum separation gel is a substance that separates serum (plasma) and blood cells (blood cells). Separating gel can not only be used with coagulant but also with anticoagulant such as heparin salt, EDTA salt and sodium citrate. The separation glue is used for the preparation of high-quality specimens and for specimen storage and transportation.
If separation gel is not used in the blood collection tube of the coagulant, although it can also accelerate blood coagulation and form a blood clot and separate the serum, but the blood clot is in contact with the serum for a long time, there will be material exchange, and the blood cells are very fragile and hemolysis is easy to interfere with the serum sample , The use of separating glue to separate the barrier can solve the problem. Generally, 0.8-1.2g of separating glue is added to each blood collection tube to form an isolation layer of at least 5mm between different components of the blood to ensure the high quality of blood samples.
The pure EDTA additive is a routine blood tube, while the use of EDTA dipotassium and serum separation gel together is suitable for the collection, transportation and storage of venous blood samples for nucleic acid testing. It is for DNA amplification testing of HBV DNA, HCV, and HIV. Serum separation gel can effectively block the interference of hemoglobin in red blood cells on nucleic acid detection experiments.
We invest a lot of manpower and material resources in serum separation gel every year. Last year we also obtained a patent due to the technical improvement of separation gel, which increased our output from 500 tons per year in the previous period to 800 tons. Sales are also large. The rate of increase is even more exported to home and abroad. No matter from which aspect, the serum separation gel is one of the blood collection tube additives. And our other products are only relative to the separation glue, which may not be so brilliant, but the market share is also commendable.
Serum is one of the main specimens tested in clinical biochemical and immunological tests. At present, the methods of obtaining serum samples in medical institutions are mainly obtained by centrifugation after collecting venous blood after the blood is completely coagulated. Under normal circumstances, it takes more than 60 minutes for an isolated blood sample to completely coagulate, or even not coagulate, which is difficult to meet the needs of rapid laboratory testing. Therefore, shortening the coagulation process and quickly separating serum samples are important factors to improve the efficiency of laboratory medicine. The accelerator was born from this.
In vacuum blood collection tubes containing coagulant, fibrin precipitated filaments and lumps sometimes appear, which is caused by the unstandardized use of coagulation blood collection tubes. Choosing a coagulant with too fast coagulation speed in the preparation of vacuum blood collection tubes will cause fibrin to shrink too fast and fragile red blood cells will easily fragment and cause mild hemolysis. Generally, the precipitation of fibrin has the following reasons:
Use procoagulant blood collection tube or separation gel to promote coagulation blood collection tube. To make the coagulant evenly distributed in the blood, it must be slightly inverted and mixed 4-5 times to make the center of the blood collection tube and the surrounding blood coagulate at the same time. If the blood is not completely coagulated and then centrifuged, it can cause the precipitation of fibrin. The upper part of the serum appeared jelly-like and fragmented, mixed with the blood. Fibrin filaments are caused by the fact that fibrin has not yet fully contracted.
Secondly, when the blood collection tube company prepared the coagulant blood collection tube, the coagulant was not sprayed enough or the prepared water-soluble coagulant was not used up on the same day, and continued use the next day, which resulted in the decrease of the coagulant effectiveness. The fibrinogen in the blood is gradually transformed into insoluble fibrin under the action of the coagulant. If the coagulant dose is insufficient or fails to extend the clotting time, centrifugation can lead to the precipitation of fibrin.
Deshengâ??s main products are blood collection tube additives. It has 15 years of R&D and production experience. Coagulant, high-efficiency coagulant powder, heparin sodium, heparin lithium, and serum separation gel are our main products for so many years. With high quality, stable performance and guaranteed after-sales service, everyone is welcome to come to exchange and cooperate.
Desheng is a new scientific and technological enterprise specializing in the research and development, production and sales of blood collection additives, chemiluminescence reagents, biological buffers and in vitro diagnostic reagents. Acridine ester series of products are the company's research and development of ultra-high sensitivity chemiluminescence substrate reagents.
Under alkaline conditions, the acridine ester molecules can be attacked by hydrogen peroxide to form dioxane, which is unstable and decomposes into CO2 and n-methylacridone in the electron excited state. When it returns to the ground state, it emits light with wavelength of 430 nm. It does not need catalyst, and the luminescence system is simple.
Desheng has seven acridine ester products with different groups: 1. Ae-nhs (traditional acridine ester); 2. Dmae-nhs; 3. Me-dmae-nhs; 4. Nsp-dmae-nhs; 5. Nsp-sa-nhs; 6. Nsp-sa; 7. Nsp-sa-adh. These acridine esters produced by Desheng have the characteristics of high purity and small difference between batches. Besides, it provides other immune reagents for matching use and provides a complete set of technical services and technical support.
There are many kinds of materials in blood coagulant, among which EDTA potassium salt should have been heard by many people, but there are many types of it, and I believe many people don't know it very well. Now we can take a look at two of its models, the difference between two potassium and three potassium.
EDTA dipotassium is also called dipotassium ethylenediaminetetraacetate dihydrate, abbreviated as EDTA-K2. Its appearance is white crystal powder, tasteless, soluble in water, and its molecular weight is 404.6. Generally used in washing powder, hand washing liquid, shampoo, chemical spray, antidote, blood anticoagulant.
Edta-k3, also known as dihydrate edta-k3, is white crystal powder, odorless, easily soluble in water and hygroscopic, with a molecular weight of 442.56. It is mainly used for anticoagulation in vitro. It is an anticoagulant additive for clinical blood test. It is used for the pretreatment of blood samples in the process of clinical blood collection and test.
Hubei Desheng biotechnology has been producing these two kinds of materials for more than ten years, with rich production experience, reliable product quality and favorable price. If you have any intention, you can call for consultation. Desheng biotechnology welcomes your call.
Blood test is a very important and widely used test item at present, which is almost accepted by most outpatient and inpatients. In the past, blood tests were all manual tests, but now it has been developed into full-automatic instrument testing, which speeds up the waiting time for the examination. EDTA dipotassium salt is an anticoagulant recommended by International Committee for standardization of Hematology (ICSH). It has good anticoagulant effect and little effect on blood cell morphology. It can keep the volume and morphology of red blood cells, white blood cells and platelets unchanged and has good stability.
Hubei Desheng biotechnology has been focusing on R & D and production of blood collection reagent for many years, and anticoagulant was the core product in the early stage of the company. After more than ten years of research and development, it has been sold to more than 100 countries around the world, most of which have been well received. Our product quality is reliable, price concessions can be discussed in detail, interested can call consultation, Desheng welcome your call.
EDTA-2K, edta-3k and edta-2na are commonly used in our series of EDTA products. EDTA is the most important chelating agent in industry, which can be used as heavy metal detoxification agent, complexing agent, antioxidant synergist, stabilizer and softener; calcium, magnesium and other metal reagents, metal masking agent. EDTA disodium is an important complexing agent, which is used for complexing metal ions and separating metal ions. Daily chemical, cleaning commonly used!
Desheng biotech is a professional manufacturer of blood collection reagents. Disodium ethylenediaminetetraacetic acid dihydrate is the additive product of blood collection reagents in the early days of our company. At present, Desheng biotech has been established for decades. We have rich experience in R & D and production of blood collection reagent series products. The product quality is reliable and the price discount can be discussed in detail. If you are interested, you can call us. Desheng welcomes your call.
In our daily life, sodium citrate appears in all kinds of raw materials around us. How many people have known about it? Are you clear about its functions, features and uses? Let's take a look at some of its secrets.
Sodium citrate is the most important citrate at present, which is mainly produced by fermentation of starch and neutralization with alkali
Desheng biotechnology is a professional manufacturer of vascular additives, in vitro diagnostic reagents, biological buffers, luminescent substrates and enzyme preparations. It has independent intellectual property rights and professional R & D and production capacity in the aspect of blood vessel additives, and can produce a variety of products. Desheng has been established for many years and has rich production experience. At present, its products are sold to more than 100 countries all over the world with excellent quality and favorable price. Interested in understanding can call consultation, Desheng welcome your call.
What are the characteristics of Schiff base, such as chelation, heat resistance, liquid crystal and intrinsic conductivity. The conductivity of Conjugated Poly (Schiff base) doped with iodine can be increased by about 8 orders of magnitude, which makes it reach the level of semiconductor. The doped materials have good stability, electrochemical activity and photoconductivity.
In the conventional state, the molecular chain of poly (Schiff base) is relatively rigid, and the intermolecular force is also strong, so the polymer is insoluble and insoluble. However, the Schiff base synthesized by using trimethylol aminomethane and other solvents directly makes the Schiff base get rid of the difficult situation of polymer processing and characterization, and the conductivity increases.
In order to improve the yield of Schiff base, it is necessary to synthesize Schiff base by the reaction of 3,5-dibromosalicylic aldehyde with trimethylolminomethane, and reduce Schiff base with anhydrous ethanol at room temperature. The results showed that the molar ratio of 3,5-dibromosalicylic aldehyde to trihydroxymethylaminomethane was 1 â?¶ 1, the reaction time was 30 min, the molar ratio of sodium borohydride to 3,5-dibromosalicylic aldehyde trihydroxymethylaminomethane Schiff base was 1.5 1, and the yield of reduced Schiff base was 81.4%.
Carbomer is a commonly used rheological additive. It has excellent thickening, suspending and stabilizing abilities. Different products have long and short rheological properties. The appropriate product can be selected according to the formula. Carbomer can improve the sensory properties of cosmetics. For example, adding carbomer gel or lotion formula will quickly decompose on the skin and other salt-containing substrates. This rapid decomposition will produce high moisturizing feeling, light spreading feeling and refreshing sense. It is the finishing touch in our cosmetics.
For cosmetics, its senses are very important. After all, cosmetics are used on parts of our body. Good sense organs give people a pleasant experience, and bad sense organs may make us lose confidence in a certain product. Carbomer plays a big role in cosmetics.
Advantages of Carbomer's finishing touch:
It does not irritate human skin and eyes. After a lot of toxicological studies, it is found that it is safer than salt;
The shampoo has high-efficiency and long-lasting suspension stability. The acrylic resin cross-linked polymer can improve the suspension of silicone oil. Compared with cellulose and guar gum, it has higher suspension stability;
Improve foam stability in low foam system;
It has salt resistance. The acrylic resin cross-linked polymer can still maintain a higher viscosity in a system with salt, and is less affected by the pH value of the solution or electrolyte concentration, and has a wider range of applications;
Easy to use, short wetting time, saving a lot of manpower, material resources and time;
Good antiseptic performance, not easy to be decomposed by microorganisms, and easy to store;
It has excellent rheology, can adjust the viscosity of the product, improve the thixotropic properties of the gel system, and improve the stability of the product.
With high rheological value, it can permanently suspend insoluble components in a low-concentration system, making the product uniform and stable, while other thickeners such as cellulose cannot provide suspension.
Carbomer uses these advantages in cosmetics, let us rush to it. The quality of Cocarbom itself also plays a key role. Good carbomer quality can achieve the desired effect of the product. There are not many domestic manufacturers that really do carbomer. And Desheng is one of the manufacturers that develops and produces carbomers, and has consistently given high evaluations under the use of many companies, whether it is product quality or product service. However, Carbomer 940 and 980 are being developed and produced by Desheng temporarily, and the supply can still meet the needs of customers. You can now apply for a free sample online for trial, please feel free to contact us if you need it.
Every biological system has a well-known buffer system to maintain the acid-base balance within a specific pH range. Our lives depend on the function of the buffer system. A buffer system is a solution that resists changes in pH when acid or alkali is added. Different buffers have different ability and efficacy for skin replacement. The high-quality Good's Buffers produced by Desheng are usually used in cosmetics, such as toners, essences, lotions, eye creams and facial masks, etc., according to your application. Here are 4 biological buffers commonly used in cosmetics.
1) HEPES (4-hydroxyethylpiperazine ethanesulfonic acid)
CAS Number: 7365-45-9
Useful pH range: 6.8-8.2
The main purpose
1. Acidity regulator, or pH control agent.
2. An effective concentration of HEPES can provide an exfoliating effect.
3. Promote the transdermal absorption of various functional ingredients in cosmetics.
2) TRIS (Tris(hydroxymethylaminomethane))
CAS number: 77-86-1
Useful pH range: 7.2-9.0
use:
1. Non-ionic surfactants-amines
2. Acidity regulator or pH control agent.
3. Excellent cleaning ability and emulsifying ability.
3) BIS-TRIS (Trimethylol) Methylamino Propane)
CAS Number: 5625-37-6
Useful pH range: 6.1-7.5
The main purpose
1. Non-ionic surfactants-amines
2. Acidity regulator, or pH control agent.
3. Excellent decontamination, dispersion, emulsification and antistatic ability.
4) Glycylglycine buffer (G-132)
CAS Number: 7365-44-8
Useful pH range: 7.5-8.9
The main purpose:
1. Adjust the pH range of the solution
2. Condition the skin, reduce pores and prevent skin aging.
3. Excellent emulsification, dispersion, antistatic, wetting, penetration, and decontamination capabilities
Precautions
External factors, water and detergents may reduce the local buffering capacity, because the elution of buffer chemicals leads to increased pH and irritant contact dermatitis. Inflammatory diseases, including atopic dermatitis, psoriasis, and acne vulgaris, increase in pH and decrease buffering capacity. For the treatment of skin diseases and aging skin, emollients with a pH value slightly higher than the average normal pH value and with appropriate buffering capacity should be used first.
Desheng is a professional manufacturer of biological buffers. There are many cooperative manufacturers in the skin care industry. In addition to the two biological buffers Hepes and Tris, it also produces many other models such as Mops, Taps, Pipes, Caps, and the core product tris buffer. Known for its ability to maintain the original color of emulsions and excellent odor suppression capabilities, it is widely used in cosmetics such as skin care lotions, sunscreens, eye makeup products, nail polish, etc. It can maintain the functional effectiveness of active substances and is a skin care product The industry's trusted choice.
Nowadays, there are still many people who have many doubts about the preparation of serum separation gel. The following will introduce a preparation method of serum separation gel.
1. Select one or more ions from quaternary ammonium salt ion, quaternary phosphonium salt ion, imidazolium salt ion and pyrrole salt ion, and select one or more from halogen ion, tetrafluoroborate ion and hexafluorophosphate ion Multiple ions, prepared into the ionic liquid;
2. Mix the carrier and the silane coupling agent uniformly to obtain a carrier-silane coupling agent blend;
3. Mix the ionic liquid prepared in 1 and the carrier-silane coupling agent blend prepared in 2 uniformly to obtain the serum separating gel.
The serum separating gel product prepared by the above method has stable performance, mature and perfect production process, good physiological inertia, thixotropy and sealing, clear and transparent appearance, and all data can meet the requirements.
Serum separation gel, a kind of raw material commonly used in hospital laboratory, is inseparable from many biochemical analyses. It is an inert polymer, insoluble in water, and has high temperature resistance, low temperature resistance, oxidation resistance, and high stability. Some manufacturers such as Desheng's separation glue also have the characteristics of anti-radiation.
With the improvement of peopleâ??s health awareness, blood testing has become more common. Serum separation gel is favored by various medical institutions. There are many manufacturers of separation gel. Due to the different requirements of medical institutions for separation gel, each manufacturer produces The components of the separation rubber are different, regardless of the transparency, color, viscosity, specific gravity and other aspects of the performance. The high-quality serum separation gel can shorten the serum separation time, and the separation gel is between the serum and blood cells, which can effectively protect the serum components, so as to realize the original tube on the machine and the original tube to save the serum for future reference, reducing the possibility of errors caused by re-transfer Sex. However, the impact of inferior separation gel on the test items cannot be ignored. The use of inferior separation gel detection can increase the triglyceride (TG) in the test results abnormally. Therefore, a comparison test must be done before using serum separation gel. The following is a quick, easy and easy-to-operate method to detect triglycerides in the separation gums of various manufacturers, as follows:
1. Apparatus and reagents: automatic biochemical analyzer, triglyceride (TRIG) reagent and calibration solution are special reagents for the instrument, 5ml disposable sterile syringe, Desheng serum separation gel blood collection tube, a certain brand of inferior serum Separation gel blood collection tube, traditional ordinary blood collection tube, 0.9% sodium chloride injection.
2. Method: The traditional ordinary blood collection tube is the control group A, the Desheng serum separation gel collection tube is the control group B, and a certain brand of inferior serum separation gel collection tube is the experimental group C. Each group randomly selects 10 blood collection tubes, each tube Add 5ml 0.9% sodium chloride injection separately, place in 37â?? water bath for 15min, centrifuge at 3000r/min for 10 minutes, measure the above tubes on the automatic biochemical analyzer, record the test results, and compare the results It can be found that TG cannot be detected in the blood collection tubes of group A and B, but different concentrations of TG can be detected in each tube of group C. This method can be used to conduct a comparison test to quickly and accurately identify the quality of the separation gel in the blood collection tube The pros and cons.
Since the outbreak of the epidemic, relevant diagnostic reagent R&D and production companies have introduced RT-PCR nucleic acid detection kits for the first time. By collecting oropharyngeal swab or nasopharyngeal swab samples, the RNA of the virus is extracted and purified for qualitative or quantitative detection. "2019 -New Coronavirus". The sample collection and storage container used in this method is a "disposable virus sampling tube". The new coronavirus nucleic acid detection, disposable virus sampling tube can be said to be indispensable. Next, Desheng intends to introduce the composition and use of the virus sampling tube.
About the composition and manufacturing of the virus sampling tube:
1. Sampling swab: The sampling swab directly contacts the sampling site, and the material of the sampling head is closely related to the subsequent detection.
2. Virus preservation solution: There are two main types of virus preservation solution widely used in the market. One is the improved virus maintenance solution based on the delivery medium, and the other is the improved preservation solution of nucleic acid extraction lysis solution.
3. Preservation tube: The material of the preservation tube should be selected carefully. There are data indicating that Polypropylene is related to the adsorption of nucleic acids, especially when the ion concentration of high tension is high, polyethylene plastic (Polyethylene) is better than polypropylene (Polypropylene). Easy to grasp DNA/RNA.
About the use of virus sampling tube:
The virus sampling tube sampling is mainly divided into oropharyngeal sampling and nasopharyngeal sampling:
1. Oropharyngeal sampling: first press the tongue with a tongue depressor, and then put the sampling swab head into the throat to wipe the bilateral pharyngeal tonsils and back wall of the pharynx, wipe the back wall of the pharynx with light force, avoid touching the tongue unit.
2. Nasopharyngeal sampling: use a swab to measure the distance from the tip of the nose to the earlobe and mark it with your fingers. Insert the sampling swab into the nasal cavity in the direction perpendicular to the nose (face). The distance of the swab should be at least half the length of the earlobe to the tip of the nose. Leave the swab in the nose for 15-30 seconds, gently rotate it 3-5 times, and withdraw the swab.
It is not difficult to see from the method of use that whether it is an oropharyngeal swab or a nasopharyngeal swab, sampling is a technical task, which is difficult and easy to contaminate. The quality of the collected sample is directly related to the subsequent testing. If the viral load of the collected sample is Low, easy to cause false negatives, difficult to diagnose.
The chemical name of the biological buffer HEPES product is: 4-hydroxyethylpiperazine ethanesulfonic acid, a white crystalline powder. It is a weak acid and a zwitterionic buffer in organic chemicals. The decomposition ability of the substance can increase with the increase of temperature, and decrease with the decrease of temperature. However, compared with other buffers, its decomposition constant does not change much with temperature. This makes HEPES buffers more effective at low temperatures. A buffer to keep the structure and function of the enzyme well. It is widely used in many fields such as clinical laboratory diagnostic reagents, life science research, pharmacy and biotechnology.
1) The research of biological buffer HEPES on promoting the stability of foot-and-mouth disease antigen.
Foot-and-mouth disease antigen is easily degraded during storage and vaccine preparation, which affects the vaccine effect. The degradation rate of the foot-and-mouth disease antigen is related to the stability of the pH value of the maintenance solution. The preparation of a maintenance solution with strong pH stability can improve the quality of the foot-and-mouth disease vaccine.
2) Preparation of a skin care product containing a biological buffer, which contains a microbial fermentation product extract, which is characterized in that it also contains a biological buffer with a mass percentage of 0.1%-3.0%. Advantages: The pH value of the product has a small drift range. Within three years after the preparation of the skin care product, the pH value can be stabilized at 6.0-8.0, which has a good protection for the microbial fermentation product extract added therein, and the microbial fermentation product extract is not Easily hydrolyzed, the anti-aging effect of the product lasts for a long time.
3) Prepare a neuron-specific enolase content detection kit, which belongs to the field of human neuron-specific enolase content detection.
This detection kit has high sensitivity and a wide detection linear range, and has the advantages of simple operation, rapidity, strong specificity, good stability and high sensitivity.
In addition to HEPES, the biological buffers produced by Desheng include Tris, Bicine, Caps, Mops, Taps, EPPS, etc.; they are mainly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. Customers in need can provide samples.
The new crown virus has broken the otherwise peaceful life and messed up people's lives. It is because of the extremely high risk of infection that everyone is afraid and cautious. When difficulties arise, methods to solve them will follow. In order to determine whether they are infected with the new crown virus, nucleic acid testing has made its debut. Nucleic acid testing is an important means to confirm the new coronavirus, and it is also one of the important processes for patients to confirm the diagnosis. To do nucleic acid testing,
Nucleic acid detection has the characteristics of early diagnosis, high sensitivity and specificity, and is the "gold standard" for diagnosing new coronary pneumonia. Currently, the most widely used real-time fluorescent quantitative RT-PCR technology. Generally, the two targets located on the ORF1ab and N genes of the virus are detected. The same specimen must meet the double target positive or the repeated test as single target positive, or the two specimens must meet the single target at the same time to confirm the positive of SARS-CoV-2 virus nucleic acid.
The virus transport medium developed by Desheng for the new coronavirus is inactivated and non-inactivated. The nucleic acid detection mainly uses an inactivated virus transport medium, which can instantly crack the pathogen and release the nucleic acid, and the protective agent can prevent the nucleic acid from being degraded. . By fully mixing the collected sample with the virus lysis solution and the virus nucleic acid preservation solution, the virus in the sample can be inactivated. Colleagues can effectively ensure the integrity of the virus nucleic acid in the sample. The collected sample can be transported and processed under normal temperature conditions. Long-term preservation. The saved viral RNA samples can be widely used in genetic testing, enzyme-linked immunoassay (ELISA), PCR testing, etc.
Although Desheng does not currently have the technology to produce nucleic acid detection kits, it can produce virus preservation solutions, disposable virus sampling tubes and biological buffers required for nucleic acid detection, as well as luminol and acridine as the raw materials for antigen and antibody detection. ester. Desheng is willing to contribute its part in the fight against the epidemic, and is willing to fight for the cause of human health.
How to use swab virus preservation solution
1. Before sampling, mark relevant sample information on the label of sampling tube.
2. According to different sampling requirements, use sampling swab to sample in corresponding parts. The specific sampling methods are as follows:
a) Nasal swab: gently insert the swab head into the nasopalatine part of the nasal meatus, stay for a while, and then slowly rotate to exit. The other swab swabs the other nostril, immerses the swab head in the sampling solution, and discards the tail. (suitable for sampling with this product)
b) Pharyngeal swab: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with swab. Similarly, immerse the swab head in the sampling solution and discard the tail. (suitable for sampling with this product)
3. Quickly put the swab into the sampling tube (including virus transport fluid).
4. Break the sampling swab at the broken place and tighten the tube cover.
5. Freshly collected clinical specimens should be transported to the laboratory within 96 hours at 4 - normal temperature, and those that cannot be transported to the laboratory within 96 hours should be stored at - 70 â?? or below. The samples should be extracted as soon as possible after being sent to the laboratory. The samples that can be extracted within 24 hours can be stored at 4. If not, they should be stored at - 70 or below
Carbomer 940 is white, loose, acidic, hygroscopic and slightly odorous, soluble in water, ethanol and glycerine
Oil. The common concentration is 0.1% ï½? 3.0%. Because there are a lot of carboxyl groups in the molecule, the water solution should pay special attention to neutralization with alkali
Use it to reduce irritation to skin and mucous membrane. Since last year's epidemic, hand gel has always been a best seller, but do you know what role carbomer 940 plays?
Carbomer 940 is used in hand sanitizer gel. The main reason is its thickening, viscosity and permeability.
Compared with hand washing, most people prefer gel products. Carbomer can turn the solution into gel, and gel is more durable and convenient to use than liquid.
We Desheng carbomer 940 products and customer praise, only the test and time witness products are good products, Carbomer viscosity can be customized according to customer requirements, need to contact our sales or online application sample trial, as long as you believe us, all your problems are not a problem.
Carbomer 980 is a polyacrylic acid cross-linked polymer that can form a high-viscosity gel at very low concentrations.
[Chinese name]Â Carbomer resin
[English name]Â Poly (acrylicacid)
[CAS No.] 9003-01-4
[Storage conditions]Â 2-8 C
[Flammability hazard characteristics]Â flammable; decomposition by heating releases irritating smoke
[Storage and transportation characteristics]Â warehouse ventilated at low temperature and dry
[Features]
Carbomer 980 is a polyacrylic acid cross-linked polymer that can form a high-viscosity gel at very low concentrations. Carbomer 980, as an efficient thickener, suspending agent and gel base, has been widely used in leave-on gel materials and cosmetics. Carbomer 980 series is white loose powder It has good thickening performance, high transparency, high viscosity, strong suspension ability, short rheology carbomer of co-solvent system, and has excellent suspension stability performance. The main characteristics are swelling and slightly acidic.
[Application]
Now the epidemic situation of New Coronavirus is becoming more and more serious, and the domestic epidemic situation has not been relaxed. People's demand for hand-washing and disinfection is increasing. With the continuous improvement of people's concept of disinfection, no-hand-washing disinfection gel is gradually in daily family life. It is more and more widely used, and Carbomer 980 is an important component of the no-clean hand disinfection gel. Its use method is simple, the effect is remarkable, and it can effectively remove the bacteria on the hand. Carbomer 980 can also be used in skin care lotions, creams, transparent skin care gels, hair styling gels, shampoos, shower gels and other products.
[Usage and precautions]
Carbomer is a cross-linked acrylic polymer that swells with water and a small amount of neutralizer (such as neutralizer and neutralizer 2) is added to form a highly transparent gel. Different types of carbomer represent different viscosities. It is known as short rheology or long rheology, for example: Carbo 940 has short rheological properties, reaching 63000MPA.S at 0.5% viscosity, suitable for products with high viscosity, Carbo 941 has long rheological properties, at 0.5% Viscosity of 7500MPA.S is suitable for low-viscosity products. Carbomer's corresponding models have ion-resistant and ion-resistant performance, daily chemical industrial products.
The Carbomer 980 developed and produced by Desheng has been unanimously approved by customers since its launch. As an excellent gel matrix, it is crystal clear and smooth to the touch. It is very suitable for preparing cream or gel because of its simple process It has good stability, is comfortable after use and is widely used. It is one of the better raw materials for hand-free disinfection gel. Welcome enterprises or personal call us for further consultation.
Desheng is a manufacturer specializing in the production and sales of carbomer and other chemical products. It has its own professional technical team and its products are exported to Europe, the United States, South Africa and other parts of the world. Our corporate tenet is based on ethics, honesty first, based on the market, strengthening our strength, and taking the road of scientific and environmentally sustainable development. Carbomer has short rheology, high viscosity, high clarity, and low ion resistance. And shear resistance, suitable for gel and cream.
Carbo, also known as carbomer, is a very important rheology modifier. The neutralized carbomer is a gel matrix and has important purposes such as thickening and suspension. It has simple process and good stability. It is widely used in lotions, creams and gels. Carbo resin can be used as an excellent suspending agent, stabilizer, emulsifier, and also as a transparent base for cosmetics and a base for pharmaceutical excipients.
Desheng currently mainly sells two models of Carbomer 940 Carbomer 980, and its business covers more than 30 countries. Most of the big customers come from Europe and the United States and other parts of the world. We can provide high-quality products and services, compared with other manufacturers Higher cost performance. Thanks to the public efforts of all employees of the company, our Kaohm sales continue to rise, and our products have been unanimously recognized by customers.
During the epidemic period, many companies closed down or fired their employees due to business stagnation. This phenomenon has never happened in Desheng. On the contrary, Desheng looked for opportunities in adversity, set up a technology research and development center, increased R&D investment, and successfully developed Kabo As a professional carbomer manufacturer, Desheng is based on the domestic market, expands the international market, sincerely serves the society, pursues high quality and high quality, and is gradually becoming a Modern, ecological and technological enterprises are welcome to consult and cooperate.
Desheng Material Technology Co., Ltd. has sufficient carbomer stocks, price reductions and sales promotion, factory direct sales, spot direct supply, avoiding the price difference between middlemen, and buying carbomer preferentially.
The article is just a brief introduction of Carbomer. For inquiries about Carbomer or if you want to know more details and specific price information, please call or enter the Desun website for detailed consultation.
This event is aimed at products, Carbomer 940 and Carbomer 980. Carbomer is routinely used in hand-washing gels, daily chemicals, and medicine. In use, we pay more attention to issues such as viscosity and transparency. The carbomer produced by Desheng Materials has the characteristics of pure white powder, high transparency, and viscosity can be adjusted according to the direction of use.
Desheng was established in 2005, specializing in the production of polyacrylic acid materials. So far, it has developed three generations of serum separation gels for different purposes and carbomer products for non-use. A professional R&D team is one of the important conditions for Desheng to be confident to gain a foothold in the market, and market feedback is the best goldsmith.
The big difference between Desun Carbomer and ordinary carbomer on the market is that in the powder state, it can be seen that the powder of Desun Carbomer is obviously whiter, and the powder is more fluffy and delicate. When configured as a solvent, the transparency is much higher than similar products. As Desheng is a direct manufacturer, individual indicators can also be customized.
It is a very good choice whether it is used by direct customers or purchased by traders. It can be purchased in small quantities or signed annual contracts to enjoy ultra-low discounts. While the product quality is guaranteed, the price is unlimited, and after-sales service to enjoy product quality problems, you can apply for direct return, exchange, or refund, real manufacturers, reliable products, choice is very important!
The only difference with imported brands is the price. The spot price of Desun Carbomer is as low as 100 yuan/kg, and the quantity is limited, so I missed waiting for the next batch. It is used in cosmetic raw materials, pharmaceutical gels, hand-washing gels, etc. For details, please contact Desheng website or the phone number reserved by this website.
Desun has been committed to the research of polyacrylic acid. In addition to creating serum separation gel series, the carbomer series is also a long-made series. With 15 years of R&D and production experience, Desun carbomer products are more trustworthy!
Carbomer products were first produced abroad, and now a foreign brand controls more than 80% of the market. The reason for this situation is nothing more than the gap in technical capabilities. After continuous research and development efforts in recent years, Desheng has finally been able to In some of these types, we have achieved the situation of "people do not have me, others have me superior", but after all, they are still lacking in reputation. The price increase is also to expand the reputation, win by quantity, and hope more customers Be able to know Desheng.
Desheng proposed that Carbomer products are offered at the lowest price, with the best product quality assurance, free samples, and perfect after-sales service. If you have any questions, you can contact a professional one-to-one sales manager for full-time service, and for product problems, technical participation throughout the process , You understand, we communicate with each other, we understand what you donâ??t understand, we teach!
As a more widely used carbomer product, retail and wholesale, small quantities and large quantities, Desheng are very welcome, and hope that all partners can take time to visit the factory to facilitate further cooperation and win-win in the future. The regular guarantee of Desun Carbomer is 10kg/bag. According to customer requirements, product indicators can also be customized and special packaging requirements can be subpackaged.
According to market price changes, this price will also increase or decrease according to the needs of each customer. Please ask all friends who need Carbomer to call for consultation. It is also very good to add a standby raw material supplier!
Some time ago, Carbomer could be described as crazy, and it was once in a state of pricelessness. This is in the final analysis inseparable from its own value. Because it is a polyacrylic acid cross-linked polymer, it is mainly used in our daily chemical products, and its wide range of uses has led to a state of shortage in the market.
Now more and more companies have joined the carbomer production industry, causing the price of carbomer to fluctuate all the time. Although the fluctuation range is not very large compared to before, the actual problems involved are indeed many. . If you want to buy the product you want at a considerate price, it may take a lot of time and experience.
Now there is good news to tell you that it will not only allow you to buy good products, but also have a considerate price.
Our Desheng company is now launching Carbomer 940 and Carbomer 980. The stock is sufficient, and the price is reduced to 100 yuan/KG for the ton level. Factory direct sales, direct supply from the spot, avoiding the price difference between the middlemen, and preferential purchase in the end. And the carbomer powder we produce is obviously whiter, and the powder is more fluffy and fine. After being configured as a solvent, the transparency is much higher than similar products. Since Desheng is a direct manufacturer, individual indicators can also be customized.
On the basis of such preferential treatment, in addition to the price advantage, we also have a strong after-sales team. Our R&D team will help you resolve any doubts about the product. Donâ??t doubt our integrity, because our service tenet of Desheng is: we donot hesitate to take on the problems that belong to us; we do our best to help customers solve problems that do not belong to us; we canot distinguish the problems of responsibility. Belongs to us.
What is the biggest headache for buyers of Carbomer 940 now? First of all, of course, quality, and second, price. When purchasing products, buyers will inevitably shop around. If the price is very low and the quality is not good, of course the customer has used it. You wonâ??t choose again after one time. On the contrary, products with lower prices and very good quality are more likely to be favored by customers. Desheng is such a direct manufacturer. It is now launching a limited-time ultra-low discount event at the end of the year, and you can enjoy 100 ton levels and above. With the ultra-low price of RMB/kg, Desheng has been repeating customers for Carbomer all the time, relying on high-quality product quality and service.
In today's raging new crown, carbomer can be used as one of the important raw materials in disposable hand sanitizers. It has super water absorption performance, thickening performance and good gel suspension performance. Very small usage (0.5%-2.0% usage) can achieve a good thickening effect. It can be said to be one of the indispensable raw materials in the field of hand-washing disinfection, and it also plays a very important role in skin care products. .
So for buyers of hand-washing disinfection gel and skin care products, what is the most important parameter of Carbomer? Of course, it is transparency and viscosity. Carbomer produced by Desheng has a very high transparency. High, the viscosity can be adjusted according to the needs of the purchaser. The quality parameters of all aspects of the product are provided with related test reports. Customers can compare their own needs to purchase and avoid detours.
The new year is approaching, and the season of high flu incidence has arrived. As the publicâ??s awareness of prevention gradually increases, the use of hand-washing disinfection gels will increase. With the gradual increase of buyers, there is bound to be a state of short supply, so take advantage For this wave of preferential activities, buyers are advised to stock up in advance. The price is an activity discount given by Desheng to increase market share. The promotion period is one month, from October 25, 2020 to November 25, 2020. There are no more misses. I hope that our customers can catch up with this wave of preferential efforts, stock up on inventory, and we will work together to prepare for a protracted battle with the new crown.
In the field of daily chemicals, there are many varieties of thickeners, why only carbomer has become in demand?
Carbomer is a polyacrylic acid cross-linked polymer, which is mainly used for thickening of daily chemical products. It can achieve the effect at very low concentration. Carbomer is usually used to increase the viscosity of cosmetics such as shampoo, shower gel, detergent, including some creams, and most daily chemical products contain this ingredient.
In the past few years, Carbomer's global market has developed rapidly, with an average growth rate of 11.98% from 2013 to 2017. In 2017, Carbomer's global revenue was nearly 736 million U.S. dollars; the actual sales volume was approximately 57,600 tons. In 2020, due to the large-scale spread of the epidemic around the world, many domestic and foreign manufacturers of Carbomer were forced to temporarily suspend business due to the epidemic, which led to a shortage of supplies of Carbomer in the market, and the price skyrocketed from the initial dozens to several hundred. , The crazy index is like a rocket launch. But after going through the madness, the current Kaboom also gradually calmed down, but under this calmness is also surging.
The new crown pneumonia epidemic hit the medical products industry by surprise. The supply of medical supplies such as masks, protective clothing, and disinfection supplies is seriously in short supply. The shortage of masks has caused dramatic fluctuations in the prices of meltblown non-woven fabrics and polypropylene upstream of masks, causing an uproar in the industry. At the same time, during the epidemic, the commonly used disposable hand sanitizers were also in short supply, and the strong demand led to the rapid increase in the price of the thickener carbomer. But the market then fell into chaos, and prices changed every day. To
As more and more companies join the carbomer production industry, the price of carbomer has been fluctuating. Although the fluctuation range is not very large compared to before, the actual problems involved are indeed many. . If you want to buy the products you want at a considerate price, it may take a lot of time and experience. Now there is good news to tell you that it will not only allow you to buy good products, but also have a considerate price.
In order to thank our new and old customers for their support and love, we at Desheng have made a great breakthrough in the issue of product quality and transparency. After the carbomer dissolves, it shows a colorless and highly transparent gel. This month, we have also adjusted the price preferentially. Carbomers above the ton level can be purchased at only 100 yuan per kilogram, and they travel on spot. If you miss this wave, you can only wait for next year. What are you waiting for, with good quality and price, make a decisive move, and we will solve any problems in the later quality.
Carbomer 940 is widely used in various daily chemical products due to its excellent thickening, suspension, and stability. It is found in our daily products such as shower gel, cream and skin care products. . Carbomer 940 became a big fire during the epidemic because of its important application in hand-washing disinfection gel. As more and more businesses invest in the production and R&D of Carbomer 940, buyers are dizzy. Is there a good supplier recommendation for Carbomer 940?
The answer is yes. After repeatedly comparing the products of different manufacturers, it is found that the Carbomer 940 produced by Desheng is very recommended. The reasons are shown in the following aspects.
1. Desheng has a strong R&D and production team
Desheng Technology was founded in 2015 and is located in the famous "city of hundreds of lakes" and "land of fish and rice"-Ezhou City. The company has a group of high-quality production personnel, a strong technical team with strong development capabilities, and can deal with and solve various problems. Complex technical issues have always been known for their good business reputation.
2. Desheng is a direct manufacturer of Carbomer 940
Although there are many manufacturers of Carbomer 940 in China, some of them are not of good quality. They just think it is the general trend and have not invested manpower, material and financial resources. Desheng has its own professional R&D team and production base, so there is no need to worry about product quality problems. Even if there is a problem in use, it can be returned without worry. Only the manufacturer can give such a service promise.
3. Carbomer 940 price discount provided by Desheng
When you are looking for a supplier of Carbomer 940, you must have repeatedly compared the prices. In the end, you will find that Desheng is the manufacturer that really takes action.
4. Desun Carbomer 940 has high daily output and can be shipped quickly
Desheng has a professional production base and adopts imported production equipment. The daily output can reach 3-5 tons, which can meet the large-volume ordering needs of customers. Whether the customer wants kilograms or tons of goods.
When many suppliers think of Carbomer 940, they first think of Lubrizol or Godsend. Because of the monopoly of Lubrizol's cooperation with the brand, many customers have lost their supply chains. Today, letâ??s take a look at other Carbomer manufacturers besides Lubrizol and Tianci.
When looking for suppliers of Carbomer 940, everyone is accustomed to searching on search engines, such as Baidu and 360. I donâ??t know if you have noticed. Deshengâ??s ranking is often relatively high. Deshengâ??s full name is Hubei Xindesheng. Material Technology Co., Ltd., established in 2005, specializes in the production and development of blood collection tube additives, in vitro diagnostic reagents, buffers, and luminescent substrates. Carbomer is the top priority of the company's products. Desheng specially hires professional and technical personnel to continuously improve product quality, and has a dedicated quality department to conduct inspections. Unqualified products will never reach customers.
In order to capture the carbomerâ??s market enthusiasm, many manufacturers purchase carbomer raw materials at low prices and provide them to purchasers, resulting in many unqualified phenomena after use. Desheng is a professional carbomer manufacturer with good quality products. Moreover, it is cost-effective. Now we are launching year-end discounts. If the order is large, the price is as low as 100 yuan/KG. Under the premise of ensuring product quality, Desheng provides perfect after-sales service, and promises that if there is any quality problem, it will be free The return and exchange provide buyers with product quality assurance and solve customers' troubles.
Although there are many series of Carbomer, Desheng mainly produces Carbomer 940 and Carbomer 980, because these two kinds of applications are the most. It should be noted that carbomer has strong moisture absorption, so it must be sealed when storing, and beware of moisture absorption and agglomeration in the air. The carbomer produced by Desheng is packed in non-toxic polypropylene plastic bags. , Moisture-proof and air-tight, and then packed in cardboard drums. The conventional packaging is 10KG/bag. Desheng can pack according to customer needs. Samples can be provided, and business friends in need can directly contact the official website customer service to obtain the use kit.
There are many kinds of blood anticoagulants in blood collection reagents, and one anticoagulant is widely used, that is EDTA potassium salt. EDTA potassium salt has a variety of models, in which potassium is used more, but how about its principle and application? There should be some people who don't know it very well. Here we can look at its application fields and principles.
EDTA dipotassium salt, also known as EDTA-K2 dihydrate, is a white crystalline powder, easily soluble in water and hygroscopic, with a molecular weight of 404.6. Deta dipotassium salt can protect blood cell composition, does not affect white blood cells, has the least impact on red blood cells, and can inhibit platelet aggregation, which is suitable for general blood test. However, apart from platelet separation test, it is not suitable for coagulation test and platelet kinetic energy test, nor for determination of calcium, potassium, sodium, iron, alkaline phosphatase, creatine kinase, leucine aminopeptidase and PCR test.
EDTA dipotassium salt as anticoagulant principle, first we understand the principle of blood coagulation, and this can be divided into three parts, 1, the formation of prothrombin activator, 2, prothrombin activator in the presence of calcium ion to make prothrombin into active thrombin, 3, soluble fibrinogen in the role of thrombin into insoluble fibrin. Fibrin is like filaments, crisscross, and contains a large number of blood cells to form a jelly like blood clot. This is what happens to blood clotting. EDTA ions in the blood can be converted into calcium antithrombin, so that the blood has a great affinity for calcium.
Carbomer 940 is a kind of polyacrylic acid crosslinked polymer, which is mainly used for thickening of daily chemical products, and can achieve the effect at a very low concentration. For example, shampoo, shower gel, detergent, and some cream cosmetics, Carbomer 940 is usually used to increase the viscosity. Most daily chemical products contain this ingredient. As such a widely used chemical product, the price of carbomer 940 is a common concern for purchasers.
Carbomer 940 is the necessary raw material for hand washing gel. During the epidemic this year, there was a shortage of supply. The price fluctuated very much. After the epidemic gradually subsided, with more carbomer 940 manufacturers being put into production, the price of carbomer 940 gradually stabilized. Desheng's carbomer 940 has the same quality as imported products.
Recently, Desheng's sales department reported that many customers didn't get a very clear reply after consulting the price of carbomer 940, so they directly went offline without any trace. Here, we should be especially fair to customer service. Online customer service can't give a very clear reply without knowing the specific order quantity. There must be differences between the price of carbomer 940 kg and the price of ton The difference is the same as retail or wholesale. Large quantities and more favorable prices are also applicable in the chemical industry. Therefore, when consulting the price of carbomer 940, it is very important to make the order demand clear.
There are a few points to pay attention to when using ECL luminescent liquid:
1. When using ECL, the previous steps can be carried out in sunlight, but the sensitivity may be slightly reduced when exposed to strong light for too long. Wearing gloves can avoid leaving fingerprints on the film.
2. If the exposure is too long or the protein is too much, the background will deepen and the band strength will lose the linear relationship, while the underexposure will blur the band.
3. The strips on the membrane glow after incubating for about 1 minute in the luminescent working solution. The strong band luminescence is visible to the naked eye in the darkroom, and the low-abundance protein band luminescence is weak or even invisible to the naked eye, but it can be exposed to X-ray film. The light emission time of the strip cannot be judged by visual observation alone.
4. Too high antibody concentration will cause high background or no bands, which will lead to failure!
ECL luminescent fluid is mainly made of luminol, isoluminol or isoluminol derivatives, sodium hydroxide and hydrogen peroxide. Desheng produces luminol and isoluminol raw materials, which need to be matched Lye, hydrogen peroxide, and peroxidase can complete the chemiluminescence experiment.
Tris saturated phenol:
1. Take out the re steamed phenol from the refrigerator and place it in a 68 water bath at room temperature to dissolve it. Do not put it in a 68 â?? water bath immediately to prevent the glass from cracking;
2. Add 8-hydroxyquinoline to 0.1% and �² - mercaptoethanol to 0.2% (stock solution 14.4mol/ml), mix well, the solution turns yellow, and pour into the separating funnel (it can also be carried out in a beaker);
3. Add 1mol / ml tris (ph8.0) of the same solvent, mix it repeatedly, and let it stand still until it is layered; release the Yellow phenol solution in the lower layer and discard the upper layer;
4. Add solid Tris about 1g / 100ml phenol, shake well and remove the aqueous phase;
5. Add 0.1mol/mltris (ph8.0) to balance several times until pH is 8.0;
6. Add 0.1mol/mltris (ph8.0) and store in brown bottle for 4 degrees;
7. If yellow disappears or pink, it cannot be used.
Tris saturated phenol is toxic and has a strong irritant effect on skin and human body, so it needs to be operated in the fume hood. Avoid harm, safety first.
English name N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt -monohydrate
Chinese name N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt -monohydrate
Abbreviation MAOS
CAS# 82692-97-5
Molecular weight 327.37225
Molecular formula C13H22NNaO5S
Storage condition 0-5, away from light and moisture
Transportation Conditions room temperature
Oxidation reaction
Application This product can be used in the water-soluble reagent for the determination of hydrogen peroxide by enzyme photometry, widely used in diagnosis and biochemical test.
Product advantage The purity is 99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on the new Trinder's reagent of chromogenic substrate. Enterprises or individuals in need can directly contact
English name N-(2-Hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt
Chinese name N-ethyl-N - (2-hydroxy-3-sulfopropyl) - 3,5-dimethoxyaniline sodium salt
N - (2-hydroxy-3-sulfopropyl) - 3,5-dimethoxyaniline sodium salt
N-ethyl-n - (2-hydroxy-3-sulfopropyl) - 3,5-dimethoxyaniline sodium salt
AbbreviationHDAOS
CAS#82692-88-4
Molecular weight313.30
Molecular formulaC11H16NNaO6S
Storage condition0-5, away from light and moisture
Transportation Conditionsroom temperature
Oxidation reaction
ApplicationThis product can be used in the water-soluble reagent for the determination of hydrogen peroxide by enzyme photometry, widely used in diagnosis and biochemical test.
Product advantageThe purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on the new Trinder's reagent of chromogenic substrate.
(English name)N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt
(Chinese name)N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt
(Abbreviation)DAOS
(CAS#)83777-30-4
(Molecular weight)341.35
(Molecular formula)C13H20NO6SNa
(Storage condition)0-5 , away from light and moisture
(Transportation Conditions)room temperature
(Oxidation reaction)
(Application)Biological research
(Product advantage)The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on the new Trinder's reagent of chromogenic substrate.
(English name)N-Ethyl-N-(3-sulfopropyl)-3-methoxyaniline sodium salt
(Chinese name)N-Ethyl-N-(3-sulfopropyl)-3-methoxyaniline sodium salt
(Abbreviation)ADPS
(CAS#)82611-88-9
(Molecular weight)295.33
(Molecular formula)C12H18NO4SNa
(Storage condition)0-5),away from light and moisture
(Transportation Conditions)room temperature
(Oxidation reaction)
(Application)This product can be used in the water-soluble reagent for the determination of hydrogen peroxide by enzyme photometry, widely used in diagnosis and biochemical test.
(Product advantage)The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on the new Trinder's reagent of chromogenic substrate. Enterprises or individuals in need can directly contact
(English name)N-Ethyl-N-(3-sulfopropyl)-3-methoxyaniline sodium salt
(Chinese name)N-Ethyl-N-(3-sulfopropyl)-3-methoxyaniline sodium salt
(Abbreviation)ADPS
(CAS#)82611-88-9
(Molecular weight)295.33
(Molecular formula)C12H18NO4SNa
(Storage condition)0-5),away from light and moisture
(Transportation Conditions)room temperature
(Oxidation reaction)
(Application)This product can be used in the water-soluble reagent for the determination of hydrogen peroxide by enzyme photometry, widely used in diagnosis and biochemical test.
(Product advantage)The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on the new Trinder's reagent of chromogenic substrate. Enterprises or individuals in need can directly contact 15071057538(Wechat is the same number) . We look forward to your calls, to join hands in creating a better tomorrow!
(English name)N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methoxyaniline sodium salt dihydrate
(Chinese name)N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methoxyaniline sodium salt dihydrate
(Abbreviation)ADOS
(CAS#)82692-96-4
(Molecular weight)347.36035
(Molecular formula)C12H22NNaO7S
(Storage condition)0-5),away from light and moisture
(Transportation Conditions)room temperature
(Oxidation reaction)
(Application)This product can be used in the water-soluble reagent for the determination of hydrogen peroxide by enzyme photometry, widely used in diagnosis and biochemical test.
(Product advantage)The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on the new Trinder's reagent of chromogenic substrate. Enterprises or individuals in need can directly contact
|English name|Sodium 3-(N-ethyl-3-methylanilino|propanesulfonate
|Chinese name|Sodium N-ethyl-N - (3-sulfopropyl| - 3-methylaniline, monohydrate
|Abbreviation|TOPS
|CAS#|40567-80-4
|Molecular weight|279.33
|Molecular formula|C12H18NNaO3S
|Storage condition|0-5 away from light and moisture
|Transportation Conditions|room temperature
|Oxidation reaction|
|Application|This product can be used in the water-soluble reagent for the determination of hydrogen peroxide by enzyme photometry, widely used in diagnosis and biochemical test.
|Product advantage|The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on the new Trinder's reagent of chromogenic substrate. Enterprises or individuals in need can directly contact
|English name|N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline
|Chinese name|N-ethyl-N - (2-hydroxy-3-sulfopropyl) - 3-methylaniline sodium salt dihydrate
|Abbreviation|TOOS
|CAS#|82692-93-1
|Molecular weight|295.33
|Molecular formula|C12H18NNaO4S
|Storage condition|room temperature, away from light and moisture
|Transportation Conditions|room temperature
|Oxidation reaction|
|Application|This product can be used in the water-soluble reagent for the determination of hydrogen peroxide by enzyme photometry, widely used in diagnosis and biochemical test.
|Product advantage|The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on the new Trinder's reagent of chromogenic substrate. Enterprises or individuals in need can directly contact
[ProductName]Sodium heparin
[Package Specification] Plastic bottle,10g/bottle,20g/bottle,50g/bottle
[Product Performance]White amor phous powder,odorless,soluble in water,easy to absorb
moisture,averagemolecularweightof15,000,stableatroomtemperature.Thepotencyof
sodium heparin four company is|150IU/Mg,and the potency of anhydroussodiumheparin
is 160IU/Mg.Otherindexeswere controlled according to sodium heparin standard.
[Scope of application]
1.This product is applicable to the collection and anticoagulation of blood samples for clinical
bio chemical examination and emergency bio chemical examination, and also applicable to the
collection and anticoagulation of blood samples for some hemorheology projects.
2.The use of sodium heparin as ananti coagulantis recommended for the determinationofion
.
|English name|3-[Tris(hydroxymethyl)methylamino]-1-propanesulfonic acid
|Chinese name|N-tris (hydroxymethyl) methyl-3-aminopropylsulfonic acid
|Abbreviation|TAPS
|CAS#|29915-38-6
|Molecular weight|243.28
|Molecular formula|C7H17NO6S
|Storage condition|room temperature, away from light and moisture
|Molecular structure|
|Application|This product can be used as a buffer system commonly used in DNA screening system and as a buffer component of RNA sample. It is suitable for the study of electron transfer and phosphorylation of chloroplast thin layer preparation. It can protect oxyhemoglobin from oxidation to methemoglobin in the process of freeze-drying. It can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.
|Product advantage|The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 15 years of R&D experience, especially has a deep research on chromogenic substrate and biological buffer. Enterprises or individuals in need can directly contact
|English name|N-Cyclohexyl-3-aminopropanesulfonic acid
|Chinese name|3-(cyclohexylamine)-1-propanesulfonic acid
|Abbreviation|CAPS
|CAS#|1135-40-6
|Molecular weight|221.32
|Molecular formula|C9H19NO3S
|Storage condition|room temperature, away from light and moisture
|Application|Tris Gly, SDS and 20% methanol buffer are not used for PVD membrane transfer of protein. Because there is glycine in the
buffer, it is difficult to distinguish whether glycine is brought in by buffer or the protein itself during sequencing. Now, 10mm / 1caps10%
methanol is used as membrane transfer buffer for sequencing, and the methanol concentration range in caps electroimprinting buffer is 0-20%.
Generally, the methanol concentration is high The buffer has a good effect on the transfer of low molecular weight protein, while the buffer with
low methanol concentration or even without methanol is conducive to the transfer of high molecular weight protein.
|Product advantage|The purity is |99%, with good water solubility and stable process, which can ensure that the product appearance is
pure white crystal powder.
Chinese Name: bis (2-hydroxyethyl) amino (trihydroxymethyl) methane
English Name: 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol
CAS No.: 6976-37-0
Molecular formula: C8H19NO5
Molecular weight: 209.24
Storage conditions: Store at RT
Purpose: It can be used in biological buffer for detecting creatine kinase. It is also used to protect hemoglobin in freeze drying. Cathodic electrolyte is used in IEF/2-D gel electrophoresis.
Chinese Name: piperazine-N, N '- bis (2-ethanesulfonic acid) sesodium salt
English Name: PIPES sesquisodium salt
CAS No.: 100037-69-2
Molecular formula: C16H33N4Na3O12S4
Molecular weight: 670.68
Storage conditions: Store at RT
Application: biological buffer
PIPES-Na
Chinese Name: piperazine-N, N '- bis (2-hydroxypropane sulfonic acid) disodium salt
English Name: Piperazine-N,N'-bis(2-hydroxypropanesulphonic acid) disodium salt
CAS No.: 108321-07-9
Molecular formula: C10H20N2Na2O8S2
Molecular weight: 406.38
Chinese Name: piperazine-N, N '- bis (2-hydroxypropylsulfonic acid) sesquisodium salt
English Name: Piperazine-N,N'-bis(2-hydroxypropanesulfonic acid) sesquisodium salt
CAS No.: 108321-08-0
Molecular formula: C20H41N4Na3O16S4
Molecular weight: 790.79
Chinese Name: disodium 1,4-piperazine diethylsulfonate
English Name: Disodium piperazine-1,4-diethanesulphonate
CAS No.: 76836-02-7
Molecular formula: C8H16N2Na2O6S2
Molecular weight: 346.33
Storage conditions: Store at RT
Application: biological buffer
piperazine-N, N '- di (2-ethanesulfonic acid)
|English name|PIPES
|Chinese name|Piperazine-N, N '- bis (2-ethylsulfonic acid) / piperazine-1,4-diethylsulfonic acid
|CAS#|5625-37-6
|Molecular weight|302.368
|Molecular formula|C8H18N2O6S2
|Storage condition|room temperature, away from light and moisture
|Application|This product can not form stable complex with most metal ions, and can be used as buffer in solution system containing metal ions. It was applied to the purification of tubulin by using cellulose phosphate chromatography. The purified recombinant GTP binding protein ARF1 and ARF2 were purified by gel filtration and used as buffer to crystallize ketoenzyme from E. coli. In addition, PIPES is not suitable for redox system because it can form free radicals. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because pipes has relatively large ionic strength and its pKa value is concentration dependent.
|Product advantage|The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on chromogenic substrate and biological buffer. Enterprises or individuals in need can directly contact (Wechat is the same number) . We look forward to your calls, to join hands in creating a better tomorrow!
piperazine-N, N '- di (2-ethanesulfonic acid)
|English name|PIPES
|Chinese name|Piperazine-N, N '- bis (2-ethylsulfonic acid) / piperazine-1,4-diethylsulfonic acid
|CAS#|5625-37-6
|Molecular weight|302.368
|Molecular formula|C8H18N2O6S2
|Storage condition|room temperature, away from light and moisture
|Application|This product can not form stable complex with most metal ions, and can be used as buffer in solution system containing metal ions. It was applied to the purification of tubulin by using cellulose phosphate chromatography. The purified recombinant GTP binding protein ARF1 and ARF2 were purified by gel filtration and used as buffer to crystallize ketoenzyme from E. coli. In addition, PIPES is not suitable for redox system because it can form free radicals. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because pipes has relatively large ionic strength and its pKa value is concentration dependent.
|Product advantage|The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on chromogenic substrate and biological buffer. Enterprises or individuals in need can directly contact . We look forward to your calls, to join hands in creating a better tomorrow!
Chinese Name: morpholine ethanesulfonic acid sodium salt
English Name: MES sodium salt
CAS No.: 71119-23-8
Molecular formula: C6H12NNaO4S
Molecular weight: 217.22
Storage conditions: Store at RT
The structure and function of the protein need to be maintained in a suitable pH environment. Therefore, the choice of pH buffer is very important during the preparation and purification of the protein. Only a suitable buffer can ensure the activity and structural stability of the protein.
Effect of buffer in protein purification process
There are many kinds of buffers for protein purification. While maintaining the pH of the system, it is necessary to ensure that the protein cannot be inactivated during the purification process, or to minimize the amount of protein inactivation, which must be maintained in every step of protein purification. Solubility and activity of protein.
Protein purification buffer requirements
The buffer in the protein purification process should not only prevent protein degradation, but also prevent protein aggregation, which has a significant impact on the experimental results. The following factors should be considered when selecting and reagent buffers: pH, buffer system, salt ions, reducing agents and stabilizers. Each of these factors should be optimized according to the purified target protein, and the activity of the target protein should be used as an evaluation criterion for optimization.
Understand protein stability conditions
There are many types of proteins, and different proteins adapt to different pH values. The buffer should be selected according to its optimal pH value. In many cases, the pH is 7.4, which is a mimic biological condition, and most proteins are stable at this pH. However, there are some exceptions, which require pH adjustment to keep the protein soluble and not degraded.
Commonly used buffers for protein purification
Commonly used buffers in the protein purification process include Tris, Tricine, Bis-Tris, MOPS, etc. Among them, Tris is commonly used. Its buffer range is close to physiological pH and has a low ionic strength. It is a good solvent for proteins. Can ensure the stability of the protein. It should be noted that when preparing the buffer, try to avoid the isoelectric point pl of the target protein. Because the protein has no electrostatic charge on its surface in the pH solution of its isoelectric point, the repulsive force between the hundred molecules is reduced, it is easy to aggregate, and the solubility is reduced.
Now the adaptation pH value, isoelectric point, and buffer range of the buffer to be purified can be found, and you can directly match it according to the corresponding requirements. Desheng is a manufacturer of in vitro diagnostic reagents, which can provide large-package pH buffers such as Tris, MOPS, Bicine, etc.
Hubei Xindesheng was established in 2017. It is a new technology-based enterprise established on the basis of Wuhan Desheng Biochemical Technology Co., Ltd. (2005). After years of innovation and development, the company has now become a leader in "blood collection tube additives", "chemiluminescence reagents", "biological buffers", "color reagents", etc., integrating R&D, production, sales and service. high-tech enterprises.
Tris, as the company's main advantage product in biological buffers, has always had its own advantages:
1. TRIS is used as a neutralizer in cosmetics, which is actually a PH regulator.
2. Tris is used as a solvent for nucleic acids and proteins and as a biological buffer.
3. It can be used to prevent and treat metabolic acidosis and respiratory acidosis;
4. Tris is used to prepare surfactants, vulcanization accelerators and intermediates for some drugs.
5. Tris mainly acts as a pH regulator, cross-linking accelerator, polyester coating raw material and phase change core material in the coating preparation process;
6. Products related to Tris can be prepared through the molecular structure of Tris. Such as Bis-Tris, Tricine, TES, TAPS, etc.
From the above points, we can see that Tris itself has a wide range of applications, but it is not a simple matter to make high-quality Tris. In the past ten years, Desheng has been learning and improving its technology. The Tris produced is white crystalline powder; it has good solubility in water systems and minimal solubility in organic solvents; it is in dry or solution state. It has excellent stability and can resist enzymatic or non-enzymatic degradation; it has a very small absorption value for visible light and ultraviolet light.
Therefore, when we store tris, we only need to store it in a cool, dry and moisture-proof warehouse at room temperature. Desheng is a professional TRIS manufacturer, committed to providing customers with high-quality products and services that can meet the special requirements of R&D customers for product types, packaging, purity and other aspects.
4-Hydroxyethylpiperazine Ethane Sulfonic Acid (HEPES) is a zwitterionic buffer, white crystalline powder, soluble in water, it is a weak acid, its pH buffer range is 6.8-8.2, it is important in biochemical industry Biochemical preparations are also considered to be one of the important buffers in the field of biological research.
As a veteran company with more than ten years of experience in producing HEPES, Desheng, the confidence we give to customers comes from our advantages in all aspects of products:
1. Advantages of PH range:
The suitable culture environment for most cells is pH 7.2-pH 7.4, and the application range of Desheng HEPES buffer just falls between pH 6.8-pH 8.2, which is in line with the characteristics of the pH value needed for cell culture.
Purity advantage:
Desheng HEPES has a purity of >=99%, good water solubility, stable process, and can control a constant pH range for a long time. According to your application needs, we can provide a full range of product testing and special testing services.
Reserve advantages:
Desheng has a professional R&D and production team, specializing in the production of HEPES, with a daily output of 1-2 tons, and advanced production technology and equipment. There will be no long supply cycle or supply outage. For customers, we enjoy the advantage of trial equipment.
Export advantages:
Desheng is a professional manufacturer of biological buffers, with strong logistics capabilities and rich export experience. We export to more than 100 countries and have established a long-term cooperation intention.
HEPES packaging advantages:
Only after using a good product can you know whether it is a good product in your mind. Desheng is not a company that only manufactures products once. What we want is the trust and support of our customers. We recommend that you use our trial pack first when purchasing the product. Because we firmly believe that quality is the greatest sincerity to give back to customers.
Luminol can be oxidized by many substances to produce chemiluminescence, which is the earliest research and most widely used luminescence reagent. The chemiluminescence analysis of metal ions occupies an important position in the entire research and application of chemiluminescence analysis. Some metal ions can enhance the chemiluminescence of the luminol system; some ions can inhibit the chemiluminescence of the luminol system; and some ions can directly oxidize the luminol to produce chemiluminescence. In our drug analysis, we use the catalysis or inhibition of metal ions and compounds for direct or indirect detection. Let's take a look at how luminol "extraordinarily" in drug analysis.
Application of Luminol Luminescence System in Pharmaceutical Analysis
1. Direct or indirect detection using the catalysis or inhibition of metal ions and metal ion compounds, such as the determination of sodium nitroprusside (SNP) and catechol compounds. Certain reducing substances such as citric acid, citrate esters, Vc, ascorbic acid, etc. can also be detected by the metal ion-luminol luminescence system.
2. The enhancement or inhibition of the chemiluminescence reaction of luminol by biomolecules and organic substances. DNA, albumin, glucose, and uric acid can enhance the chemiluminescence reaction of luminol, thus establishing their own detection methods. In an alkaline medium, the inhibitory effect of ascorbic acid on the Luminol-K3Fe(CN)6 system can be used to determine ascorbic acid. Luminol-K3Fe(CN)6 series can also be used to monitor glucose and lactic acid. Thiol-containing drugs, promazine, etc. have an inhibitory effect on the luminol luminescence system, and a detection method is proposed accordingly.
3. Preparation of expendable chemiluminescence sensor. Luminol and other reagents are fixed on ion exchange resin to develop a chemiluminescence flow injection sensor, which is used for the determination of Vc. Combine enzyme immobilized column with FIA, use immobilized enzyme reactor, detect by luminol, it has been used for glucose, alcohol, oxalate, lactate, choline, uric acid in serum and urine, etc. Detection.
Seeing more of the power of luminol, we suddenly discovered that it is acceptable to us wherever it is. This also fully illustrates its value. If you want to know more about luminol's knowledge and uses, you might as well find its manufacturer-Desheng. I believe you will be worthy of asking.
What is PIPES buffer
PIPES Buffer (CAS5625-37-6) chemical name is 1,4-piperazine diethanesulfonic acid, which is a zwitterionic biological buffer. It is a white powder solid at room temperature. PIPES Buffer is not easily soluble in water (1g/L (100C). It can be dissolved by mixing equimolar sodium salt solution and titrating to the appropriate pH value. For example, PIPES Buffer (CAS76836-02-7) and PIPES sesquisodium salt ( CAS100037-69-2).
Application of PIPES Buffer
PIPES Buffer is widely used in cell culture, chromatography, cosmetics, diagnostic testing, protein purification and electron microscopy research. PIPES Buffer is similar to MES Buffer and MOPS Buffer (also produced by Desheng). This Good's Buffer lacks the ability to form complexes with most metal ions, so it is recommended to be used as a non-coordinating buffer in metal ion solutions.
Advantages of PIPES Buffer
According to related literature, the use of NaOH-PIPES buffered glutaraldehyde solution in the ultrastructure of various tissues, such as human skin, human hypertrophic scars, mollusk neuron tissue, etc., the results show that more organelles and organelles can be observed Between the details. The cytoplasm is more uniform in consistency. Compared with NaOH-PIPES buffer, microfilaments and microtubules are more common in PIPES tissues. In addition, PIPES Buffer is proven to be comparable in living cells.
What is TRICINE buffer
TRICINE Buffer (CAS 5704-04-1), a zwitterionic biological buffer containing amine and acid groups. This product is a white crystalline powder, slightly soluble in water (the solubility is 1.8g/100ml water).
Application of TRICINE Buffer
TRICINE is widely used in cell culture and electrophoresis research. As a buffer for cell culture research, TRICINE can inhibit the unexpected growth of mycoplasma in animal tissue culture. It has also been used in bacterial culture to prevent precipitation of iron salts. The Good's buffer can form complexes with most metal ions, such as Mg, Ca, Co, Cu, Ni, and Zn, so the stability constant and concentration should be considered when choosing the buffer. As a buffer for electrophoresis research, Tricine has been used as an alternative to protocols using the controlled substance barbital.
Advantages of TRICINE Buffer
TRICINE has a higher negative (more negative) charge than glycine, making it migrate faster. In addition, its high ionic strength results in more ion movement and less protein movement, so that low-molecular-weight proteins can be separated in a lower percentage of acrylamide gels. Tricine has been proven to be used to separate proteins in the range of 1 to 100 kDa by electrophoresis. In ten tests using firefly luciferase for ATP determination, 25mmol/L tricine buffer was found to be the most effective buffer.
1. Basic nature
The application of acridine compounds in chemiluminescence detection has the following advantages:
Low background luminescence and high signal-to-noise ratio;
Less interference factors in luminescence reaction;
The light release is fast and concentrated, the luminous efficiency is high, and the luminous intensity is large;
It is easy to link with protein and the photon yield does not decrease after linking;
The markers are stable (storable for several months at 2-8C), they have good solubility in water, are stable and are not easily hydrolyzed.
The chemiluminescence of acridine compounds does not require a catalyst and can emit light in a dilute alkaline solution containing H2O2. The luminescence of the compound is flash type. After adding the luminescence starter, the emitted light intensity reaches the maximum in about 0.5s, and the half-life is about 1s.
Acridine compounds are used in chemiluminescence immunoassay (CLIA). That is, the antibody or antigen is labeled with acridine salt, and HNO3+H2O2 and NaOH are used as the luminescence priming reagent. Some add Triton X-100, CTAC, Tween-20 and other surfactants to the luminescence priming reagent to enhance luminescence.
Acridine compounds are commonly used in environmental analysis, immunoassay, enzyme activity analysis and labeling oligonucleotide fragments, etc., and are used clinically to determine bacteria, viruses or other disease markers.
2. Acridine ester DMAE-NHS or NSP-DMAE-NHS
Acridine ester DMAE-NHS or NSP-DMAE-NHS is an ideal chemiluminescence immunoassay labeling substance with good luminescence performance. Compared with the widely used acridinium ester AE-NHS, the luminescence intensity of the acridinium ester DMAE-NHS or NSP-DMAE-NHS and antibody (Ab) conjugates is higher, and the luminescence dynamics of the two are very similar. Both are flash-type luminescence and both reach the highest at 0.4s, but the thermal stability of DMAE-NHS or NSP-DMAE-NHS is significantly higher than that of AE-NHS. AE-NHS is only stable in acidic solutions, when pH>6.3 It is easy to hydrolyze later, but DMAE-NHS or NSP-DMAE-NHS is not. For example, at room temperature, it is very stable in a PB buffer with a pH of 7.0. After 16 days, the luminescence activity is only reduced by 3.6%. Their luminescence thermal stability with protein conjugates is similar to this.
The acridinium ester DMAE-NHS or NSP-DMAE-NHS is easy to label biomolecules and the luminescent activity and biological activity of the labeled compound are stable. The synthesized luminescent label is applied to chemiluminescence immunoassay with high sensitivity and other ideals. For example, the minimum detection limit of TSH is 0.007 mIu/L. Therefore, the acridinium ester DMAE-NHS or NSP-DMAE-NHS has good application value for the research of chemiluminescence analysis and chemiluminescence immunoassay.
3. Acridine Sulfonamide NSP-SA-NHS
Acridine sulfonamide is a very effective
EDTA-2Na is a white crystalline powder in appearance, it is odorless, tasteless, soluble in water, and extremely difficult to dissolve in ethanol. In industry, it is mainly used as a chelating agent, and also as a heavy metal detoxifier, complexing agent, antioxidant synergist, stabilizer and softener, etc.; as an anticoagulant in blood collection tube additives. When in use, EDTA-2Na is generally configured as a solution, so various problems will inevitably be encountered in these configuration solutions. Let's take everyone to understand how to solve these problems.
How does EDTA-2Na dissolve? What to do if it is insoluble?
It can be dissolved at room temperature, but the speed is very slow. EDTA has a low solubility in water and is only soluble in alkaline, so NaOH should be added.
Can EDTA-2Na be dissolved by heating?
EDTA-2Na uses water as the solvent and is stirred and dissolved at about 90C. Generally, there will be no chemical changes. It can be dissolved in boiling water at 160C and slightly soluble in cold water. EDTA can be dissolved in sodium hydroxide, sodium carbonate and ammonia solution, and neutralization reaction will occur. It is insoluble in alcohol and general organic solvents. It can chelate with calcium and magnesium ions. The melting point is greater than 220C and will decompose.
How to dissolve EDTA-2Na completely?
The solubility of EDTA-2Na in water is about 110g/L. It dissolves slowly at room temperature. Heating can accelerate the dissolution, and it will not precipitate when cooled after dissolution.
Is EDTA-2Na sticking to the skin harmful during operation?
In fact, our EDTA-2Na can adjust the pH of cosmetics in skin care products, that is, it can automatically adjust the pH of cosmetics within the range that we can fit, so as to protect our skin from strong irritation. Therefore, in a certain amount of circumstances, it plays a role in protecting the skin, so you don't have to worry too much about getting it on your hands accidentally during the experiment. Just rinse it with water.
Something about acridinium ester (NSP-SA-NHS)
Acridine esters (NSP-SA-NHS) CAS: 199293-83-9 and related compounds have been proven to be very advantageous chemiluminescent labels, with stability, activity and sensitivity exceeding those of radioactive isotopes. Under alkaline conditions, the NHS will leave and the acridine ester will bind to the protein with a stable amide bond to form an acridine compound. After the reaction is completed, the excess acridine salt is removed through a desalting column. In the presence of alkaline hydrogen peroxide, acridine-labeled protein does not require enzymatic catalysis to emit light on its own. After adding the excitation reagent, the system immediately releases photons, which can be detected with a 430nm standard photometer. This light-emitting process is very short (the entire process occurs in less than 2 seconds), and the trigger scheme must add an internal photometer and photon detector. Proteins, peptides, antibodies, and nucleic acids can all be labeled with acridine. The labeled compound emits light rapidly under the excitation of basic hydrogen peroxide, and the labeled compound can be detected by collecting photons.
Product stability:
1. It is stable in acidic solution (pH4.8, especially in alkaline solution, the acridine compound is partially hydrolyzed to reduce its stability, and the hydrolysis process is a dark reaction process that does not emit light; the degree of hydrolysis increases with the increase of pH, and the degree of hydrolysis increases with the increase of temperature. Increase and increase.
3. The stability of acridine amide is higher than that of acridine ester structure, and the ability to resist hydrolysis is stronger.
4. Acridine ring or phenol ring or benzene sulfonyl ring is connected with a methyl group and other electricity-donating groups. Due to the large steric hindrance, the thermal stability is increased; the electron withdrawing group is attached, which is beneficial to the nucleophilic substitution reaction Its stability decreases.
5. Suggestion: Use a weakly acidic buffer as much as possible for the storage buffer after coupling, and bubbling nitrogen to remove oxygen. Store at low temperature, sealed and protected from light. Conditions can be made into freeze-dried samples and then stored.
Precautions:
5. The acridinium ester luminescence process is very short (the whole process takes less than 2 seconds), and the trigger scheme must add an internal photometer and photon detector; in addition, this product can also use a multifunctional microplate reader equipped with an autosampler Perform luminescence data collection to minimize the influence of sample addition time on the collected signal.
Acridine Ester Luminescence Analysis Study on DNA Damage of Formaldehyde and Acetaldehyde
Acridine ester can be used as a chemiluminescent indicator with a good intercalation structure of DNA double helix. Because low concentration of formaldehyde causes DNA breakage and damage, high concentration of formaldehyde causes DNA strand cross-linking, and acetaldehyde only causes DNA strand breakage damage. The chemiluminescence detection method for DNA break damage has the advantages of simplicity and sensitivity, and provides a simple research method for the study of environmental pollutant damage to DNA.
Acridinium-labeled streptavidin
Acridine ester DMAE -NHS was used to label streptavidin, one strain of TSH monoclonal antibody was labeled with biotin, and the other strain of TSH monoclonal antibody was coated on 96 micro-well plates, and the TSHBAS-CLIA method was established. Acridinium ester-labeled antibody or antigen, biotin-labeled antibody or antigen, streptavidin-coated magnetic beads with carboxyl or Tosyl (p-tosyl) functional groups; the particle size of the magnetic beads is 0.1-5um . Since biotin and streptavidin are combined in a ratio of 4:1, the acridinium ester-biotin-antigen-antibody complex is also combined with streptavidin in a 4-fold ratio, so that the luminescence signal is also enlarged. 4 times, the detection sensitivity is higher.
Acridine ester-labeled antibody for rapid determination of antibody level in tuberculosis patients
Using acridinium ester-labeled goat anti-human IgG antibodies, a method for the determination of IgG in human serum has been established. The labeling method has mild conditions, is simple and easy to implement, and its standard curve ranges from 7.5 to 125 ng IgG/ml. The results of the determination of antibody levels in the serum of tuberculosis patients are good.
Acridine ester-labeled nucleic acid probe method can quickly detect Escherichia coli
Use the nucleic acid probe method to quickly detect E. coli O157:H7. The method is to detect E. coli O157:H7 by using a specific DNA probe labeled with acridine ester. The nucleic acid probe method detects E. coli O157:H7 with high specificity and sensitivity. The minimum concentration of E. coli O157:H7 is about 106cfu/ml. The result of detection of E. coli O157:H7 is consistent with the national standard method; for O157 : H7 identification time is only 30 minutes, which is simple and quick. The conclusion is that the nucleic acid probe method can be used for the rapid detection of E. coli O157:H7.
In many biological experiments, buffers are needed to maintain an effective pH value, and buffers are critical to the success or failure of the experiment.
When choosing a buffer, first consider that the pH value in the experiment is consistent with the pH value range of the buffer, and consider whether the advantages and disadvantages of different buffers are suitable for your experiment. There are many types of biological buffers. In this article, we mainly discuss the preparation methods of several commonly used biological buffers.
Prepare TRIS base and TRIS-HCL buffer
Tris: To prepare 1 liter of 1M Tris buffer, first prepare to dissolve 121.14 grams of Tris powder in 750 mL of dH20, adjust to the desired pH value with concentrated hydrochloric acid, and fill to the final volume of 1L with dH2O.
Tris HCl: To prepare 1 liter of 1M Tris HCl buffer, first dissolve 157.60 g of Tris-HCl in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill with dH2O to a final volume of 1L.
Prepare HEPES buffer
To prepare 1 liter of 1M HEPES buffer solution, first dissolve 238.3 grams of HEPES powder in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, fill with dH2O to a final volume of 1L, and pass through a filter or autoclave The device is disinfected. Store the buffer at 4C.
Prepare PIPES buffer
To prepare 1 liter of 1M PIPES free acid buffer solution, first add 302.37 g of PIPES free acid to 600 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill to the final volume of 1L with dH2O.
Desheng is a professional manufacturer of biological buffers. At present, the company sells buffers such as TRIS, HEPES, PIPES, CAPS, MOPS, BICINE, etc., with sufficient supply, fast delivery, guaranteed quality, and a professional after-sales team. If you answer any questions, you can now apply for a free sample online for trial. Please feel free to contact us if you need it.
Tris, or tris(hydroxymethyl)aminomethane, has been widely used as a pH buffer in biological media for about 35 years. The near-ideal properties of this biological buffer are the reason for its popularity. Tris does not absorb water, is easily soluble in water, and has high purity. It does not precipitate calcium salts, is stable in solution for several months at room temperature, and does not seem to inhibit many enzyme systems. However, due to the chemical and physical properties of Tris buffer, improper use may lead to erroneous pH measurement of the electrode system.
The influence of pH in TRIS buffer on electrode
Biochemists use TRIS buffers to control the pH within the physiological range (approximately 7 to 8 pH), because phosphate can cause adverse reactions with biological substances in the test sample. However, when pH measurements are to be made on these solutions, another type of "adverse side reaction" must be recognized, involving the pH electrode system.
The influence of chemical and physical properties of Tris buffer on pH
Tris, which is commonly used for physiological measurement (pH 7 to 7.5), does not have a large buffer capacity. Therefore, caution should be exercised when using tris buffer to control sample pH. The rather large temperature coefficient of tris (-0.028pH/ C) is also worth considering. For example, if the meter is calibrated with tris buffer and the tris temperature is 20 C, using a pH value of 25 C will cause errors. Therefore, temperature pH dependence must be used when using tris for standardization. When using tris to control the pH of the sample, the degree of control will depend on the sample temperature. If the sample temperature changes by 2 C, the pH control changes by about 0.06 pH units. The required pH control will determine the maximum allowable temperature fluctuations.
Advantages of Tris buffer
Because the Tris base is highly alkaline, this buffer system can be used to prepare buffers with a wide range of pH values from acidic to alkaline;
It has little interference to the biochemical process, and does not precipitate with calcium, magnesium ions and heavy metal ions.
Disadvantages of Tris buffer
The pH value of the buffer is greatly affected by the concentration of the solution. The buffer is diluted ten times, and the pH change is greater than 0.1; the Tris buffer should be prepared from high-purity tris and HCl using distilled or deionized water without CO2.
The temperature effect is large, and the temperature change has a great influence on the pH value of the buffer, so it must be prepared at the use temperature, and the Tris-HCl buffer prepared at room temperature cannot be used at 0 C to 4 C;
It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed;
This buffer has a certain interference effect on some pH electrodes, so use an electrode compatible with Tris solution.
Blood analysis is a very important and powerful diagnostic tool in animal health and welfare control. It is usually performed in higher vertebrates, and its reference value has been established, but fish hematology still needs further research. Many internal and environmental factors have a profound impact on the hematology value of fish, making it difficult to determine the reference value. In addition, due to the short clotting time, fish blood usually requires the addition of anticoagulants. The choice of anticoagulant is essential to obtain reliable blood test values. In this study, the effects of two common anticoagulants, K 2 EDTA (1.8 mg/ml) and lithium heparin (18 IU/ml), the hematological value of brown trout in the breeding season on the spawning season The spawner conducted an investigation.
The results of basic hematology analysis, such as compacted cell volume (PCV), hemoglobin concentration (HGB), red blood cell count (RBC), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and White blood cell count (WBC) was compared between K 2 EDTA and lithium heparin. Statistically significant differences were observed in PCV, MCV, and MCHC, while HGB, RBC, MCH, and WBC did not show such differences. These results indicate that lithium heparin provides more reliable results because the red blood cells in the K 2 EDTA-treated samples have a tendency to swell.
The salt of ethylenediaminetetraacetic acid (EDTA) is recommended by the International Committee for Standardization in Hematology (ICSH) and the Clinical and Laboratory Standards Institute (CLSI) as the anticoagulant of choice for blood counts. EDTA acts as a chelating agent for Ca 2+ and Mg 2+ ions. It binds to calcium and is essential for the enzymatic reaction in the coagulation cascade, resulting in anticoagulation with minimal impact on cell morphology. EDTA can most reliably preserve the cells on the stained blood membrane. It is commonly used for routine hematology tests in humans, mammals and other vertebrates. It has also achieved some success in fish. However, EDTA salt has some disadvantages. In some cases, they can cause blood hemolysis, especially in specimens anesthetized with unbuffered tricaine mesylate (MS 222).
Lithium heparin is more suitable for hematological evaluation of spawning in trout than dipotassium ethylenediaminetetraacetate. Heparin does not affect the size of red blood cells, thereby minimizing the possibility of hemolysis. Compared with heparin, blood smears made from blood treated with EDTA are of higher quality. Based on current research, we suggest that K2EDTA can be used cautiously for blood count and HGB determination. In order to better understand the effects of anticoagulants on hematological parameters, further research is needed.
1. Tris brief introduction
As the handling in biological buffers, tris plays a more important role, not only because of the wide pH range of tris, it can act as a pH regulator. Conventionally, it is white crystal particles, but there are certain requirements for water solubility, and white powder will not meet its requirements better. The pH value of the acid-base indicator is 7-9, and there are other names, tromethamine, ammonium tromethamine, trishydroxymethylaminomethane and so on. Although they are all the same product, different buyers will call them differently based on different fields.
Second, the use of Tris
2. Tris application in cosmetics
With the improvement of peoples quality of life, more and more attention is paid to spiritual life. Among them, the heart of beauty is common to everyone, regardless of men and women: appearance has become an important indicator of first impression (of course I strongly disagree Because I dont have that beauty. The rise and mass use of cosmetics have become inevitable. To make good cosmetics, the ingredients are very important.
The role of Tris in cosmetics is basically to adjust the pH, and it can also be used as a neutralizer. The PH value of healthy Asian skin is slightly acidic, and the ingredients in many cosmetics are very complicated. If you want to adjust the pH value, you must use tris to adjust, so that the entire raw material composition will not change too much. Many people see that tromethamine is a chemical product, and it is very scary to use in cosmetics, and they are afraid of being unsafe. In fact, if the proportion of ingredients is used properly, there is no need to worry about it. Generally, it will not harm the skin.
3.Tris is used for synthesis
Tris with a relatively simple structure can synthesize many products, and the same buffer system, Tris-HCL, Bis-Tris Tricine, TES, TAPS, etc. can all be synthesized with tris. Compared with others, the price of Tris will be cheaper, but the synthesized products can be applied to more fields, and the price will be doubled. Therefore, the synthesis of tris can be said to be a hot pastry.
[Product Name] Serum separation gel (radiation resistant)
[Specification] 20kg/barrel, 25kg/barrel
[Product Performance] The product is colorless and transparent colloid with light smell and no impurities; it is physiologically inert and insoluble in water, with a relative specific gravity of 1.045-1.065g/cm3, a viscosity of 100,000-300,000 centipa, and a volatile content of â?¤ 1.5%. It has excellent thixotropic and isolation properties. See the product test report for other properties.
[Scope of application] This product is specially used for manufacturing disposable vacuum blood collection tube and injection blood collection tube, as a necessary materials for the storage, transmission and transfer of high-quality serum and blood samples in clinical test.
[Warning and prevention]
1. When it is found that there are impurities, foreign matters, abnormal smell, abnormal color and expiration date in serum separation gel, it is forbidden to use.
2. This product is inert, safe and non-toxic.
3. This product should not be mixed with products of other manufacturers, which may cause colloid stratification, turbidity and other phenomena.
4. Above the recommended storage temperature limit may damage the quality of the gel.
[Storage conditions and methods]
Unless otherwise stated on the package label, it is generally recommended to store in a sealed manner at a temperature of 0-40â?? (32-104â??) to avoid direct exposure to air and sunlight.
[Validity] 36 months
Desheng is an established blood test reagent company with 14 years of R&D and production experience. It has a lot of research on blood collection additives. Enterprises or individuals in need of serum separation gel can directly contact 15071057538(Wechat same number) .
When it comes to anticoagulants, many people don't understand what they are used for. Simply put, anticoagulants are chemicals that prevent blood clotting. It is very commonly used in blood collection tubes used in hospitals to collect blood for testing. In different blood tests, the applicable anticoagulants are also different. Commonly used anticoagulants include EDTA, sodium citrate, heparin, etc. Let's take a look at the mechanism of action of these anticoagulants.
Because blood coagulation is divided into three main stages: first, prothrombin activator is formed; second, prothrombin activator catalyzes the conversion of prothrombin into thrombin; third, thrombin catalyzes the conversion of fibrinogen into fibrin . In these three stages, the participation of calcium ions is required, so once the chelated calcium ions are removed, the blood loses the necessary conditions for coagulation and cannot be coagulated.
1. EDTA (ethylenediaminetetraacetic acid)
There are three different forms of EDTA ethylenediaminetetraacetic acid anticoagulant, namely EDTA dipotassium, EDTA tripotassium, and EDTA disodium. Tripotassium EDTA is relatively easier to dissolve and is more popular than other forms. EDTA plays an anticoagulant effect mainly by chelating calcium ions and removing free calcium ions.
2. Sodium citrate
Sodium citrate combines with free calcium to form a sodium citrate complex, which consumes calcium to achieve anticoagulant effect. The recommended anticoagulant concentration is 3.2% or 3.8%. The ratio of anticoagulant to blood is 1:9 and 1:4. Blood after sodium citrate anticoagulation cannot be used for compressed cell volume (PCV), hemoglobin (Hb) estimation, total white blood cell count TLC, and differential white blood cell count (DLC) because citrate is used as a solution and it changes blood concentration.
3. Heparin
Heparin is an acid mucopolysaccharide with affinity for blood proteins and can be used as antithrombin and antithromboplastin. It prevents the conversion of prothrombin into thrombin, thereby playing an anticoagulant effect. As an anticoagulant, heparin is often used in emergency biochemistry and blood rheology testing.
|English name|3-Morpholinopropane sulfonic acid
|Chinese name|3-Morpholinopropane sulfonic acid
|Abbreviation|MOPS
|CAS#|1132-61-2
|Molecular weight|209.26
|Molecular formula|C7H15NO4S
|Storage condition|room temperature, away from light and moisture
|Application|Mops is a kind of biological buffer, which is often used to prepare RNA electrophoresis buffer.
|Product advantage|The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
Desheng is a chemical reagent company specializing in blood test reagents with 14 years of R&D experience, especially has a deep research on chromogenic substrate and biological buffer.
Food safety issues have not only seriously affected the health of the people's diet, but also hindered the export trade of my country's agricultural products and food, and undermined the international reputation of my country's food industry. Only by strengthening basic food safety research, especially rapid testing research, can we adapt to the development under the new international situation. How to quickly and effectively do a good job of testing, we have to bring up our powerful chemiluminescence immunoassay to see through which aspects it plays a role in food safety testing.
Detection of microorganisms in food
The inspection of microorganisms in food is mainly to inspect general pollution and pathogenic bacteria. Escherichia coli O157 H7 is an important food pathogen. The enhanced luminol peroxidase luminescence system can quickly detect E. coli O157:H7. The method is simple and fast. The minimum detection concentration of each bacteria is in the range of 104105CFU/L, and the recovery rate is between 90% and 120%.
Detection of biotoxins in food
Biotoxin is a general term for a large class of biologically active substances, including animal toxins, plant toxins and microbial toxins. Biotoxins have posed a serious threat to food safety and human health. For example, the chemiluminescence enzyme immunoassay method for detecting aflatoxin M1 in milk uses horseradish peroxidase to catalyze luminol to emit light.
Detection of pesticide residues in food
Pesticide residues in food can cause chemical food poisoning, and low-concentration residues are also potentially harmful to the human body. At present, the most researched pesticide residue detection technologies are mainly in vivo detection methods, chemical detection methods, enzyme inhibition methods and instrumental analysis methods. modified products
After recognizing the major changes brought to us by chemiluminescence reagents, Desheng has independently developed and produced chemiluminescence reagents since 10 years ago, including luminol, isoluminol, and acridinium ester and acridinium ester derivatives. product. These products are not only recognized by many companies in China, but are also well received even if they are exported overseas. Low-key life, high-profile work. What's more, a good product in the eyes of the public always speaks with strength.
Luminol has become one of the most widely used chemiluminescent reagents due to its advantages of high quantum yield, easy synthesis, and good water solubility. Its development process is the same as the arduous process of human beings. From the beginning, there is not much understanding, to the current glow and fever, it can only be summarized in one sentence: gold always shines. In the future, there may be more uses we don't know to benefit our lives. We only need to learn more about it to discover its unlimited potential as soon as possible.
Application in the analysis of inorganic substances
Using inorganic ions to enhance or inhibit the chemiluminescence of the luminol system, these metal ions can be measured. It is worth pointing out that some metal ions have higher catalytic activity than metal ions after being combined with complexing agents. For example, using the luminol-H2O2-silver phenanthroline system, the detection limit of silver ions can reach 2.7 10-g/L3.
The use of sensitizers can improve the sensitivity of detection. The sensitization effect of triphenylmethane derivatives on the luminol luminescence system. It was found that Coomassie Brilliant Blue G250 had the best sensitization effect on the luminol-H2O2-Cu2+ system. A reverse flow injection technique was used to establish a measurement of Cu2+. The new method has a detection limit of 15. The use of the local concentration effect of surfactants to achieve micelle sensitization has been increasingly used in luminescence analysis. For example, the reversed micellar system FIA method is used to measure V4+, the detection limit is
Luminol chemiluminescence analysis has been widely used in various fields, and new methods and new technologies are constantly emerging, especially the combination with FIA, HPLC and other technologies, which overcomes the shortcomings of poor selectivity in luminescence analysis and makes the analysis more flexible. Emblem and accuracy. Because of Rumi's stable performance and low price, practical application research and analysis will have broader application prospects.
The new Trinder's reagent is a highly water-soluble aniline derivative that has been widely used in diagnostic tests and biochemical tests. In the colorimetric determination of hydrogen peroxide activity, it has many advantages compared with conventional color reagents. The new Trinder's reagents are stable enough to be used in solutions and test line inspection systems.
Currently chromogen substrates (new Trinder's reagents) include TOOS, TOPS, ADOS, ADPS, ALPS, DAOS, HDAOS, MADB, MAOS, etc. These new chromogens actually have similar reaction principles to traditional chromogens, but they have more advantages. We mainly understand the MAOS among them.
The full name of MAOS is N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt. It is a highly water-soluble aniline derivative of the new Trinder's reagent. Which has a pivotal position. It is a water-soluble reagent that can be applied to the enzymatic photometric determination of hydrogen peroxide, and is widely used in diagnostic testing and biochemical tests.
Reasons for the discoloration of the reagent solution: Color-developing substrates such as MAOS are all new Trinder's reagents, which are derivatives of highly water-soluble aniline sodium salt, which will produce red or yellow substances after being oxidized. Trinder's reagent powder is not easy to be oxidized. It needs to be sealed and refrigerated after preparation. If the solution is exposed to the air for a long time and the temperature is high, they may be slowly oxidized by the oxygen in the air. Discoloration of the solution occurred.
MAOS is sensitive to light and sensitive to humidity. Therefore, when we store, the storage temperature of cold storage is between 0-10, and the temperature of frozen storage is below 0. If the storage temperature is not specified, in principle, it should be stored in a cool place below 15C.
Hubei Xindesheng has focused on the research and development of a batch of new Trinder's reagents and other color reagents. The new reagent has more advantages: such as high water solubility, UV absorption of the color reaction product> 540nm; color reaction requires a wide pH range, and the activity of a variety of enzymes can be guaranteed during the reaction; color reaction sensitivity is higher and can be used For the determination of very small amounts of analytes.
And it has considerable product advantages, purity: >99%, good water solubility, stable process, and can ensure the appearance of the product is pure white crystal powder. Welcome everyone to inquire.
With the repeated reversal of the epidemic, the fight against the epidemic has become our primary concern for human beings. We must not only prevent the spread of the epidemic, but also protect our own safety, especially the staff who collect virus specimens. If the virus is not collected properly, it is very likely to be infected. Therefore, under such severe tests, the emergence of disposable virus storage tubes has greatly reduced the risk of infection and guaranteed the safety of our frontline personnel.
Specimen collection method
1. Pharyngeal swab: Use two plastic rod swabs with polypropylene fiber heads to wipe both pharyngeal tonsils and posterior pharyngeal wall at the same time, and immerse the swab head in 3ml virus preservation solution (isotonic saline solution, tissue culture can also be used) Or phosphate buffer), discard the tail, and screw the cap tightly.
2. Nasal swab: Gently insert a plastic rod swab with a polypropylene fiber head into the nasal palate in the nasal passage, stay for a while and then slowly turn it out. Take another plastic rod swab with polypropylene fiber head and collect the other nostril in the same way. The two swabs mentioned above were immersed in the same tube containing 3ml sampling solution, the tail was discarded, and the tube cap was screwed tightly.
3. Use an oily marker on the PE tube label to indicate the type of sample, sampling time, number, and patient name.
4. After collection, put the disposable virus storage tube in the special transport box (or vaccine transport box), put it in the ice tray, fill it with soft material, and seal it, and it will be transported by a special person instead of mailing. It is best to use a special vehicle.
5. If the collected specimens cannot be delivered within 24 hours, they should be stored below -70�°C as soon as possible. If there is no condition of -70â??, it should be temporarily stored in the refrigerator at 4â?? for a short time, and the specimen should be contacted as soon as possible.
6. Influenza virus is unstable at -20�°C to -40�°C, so long-term storage is best performed below -70C.
In this case, it greatly protects us, and it also protects the virus from degrading and causing false negatives. One-time virus preservation tube is a hero to defend in the epidemic.
What is nucleic acid detection, that is, after collecting human secretions, under laboratory conditions, by analyzing the RNA gene sequence of the virus, using PCR amplification method for detection, and diagnosis of clinical etiology. At present, nucleic acid testing is the most effective testing method for early determination of whether a patient is infected with the virus, early detection and early treatment.
Nucleic acid detection swabs are divided into two types: nasal swabs and throat swabs. During the sampling process, the doctor will use a swab like a cotton swab to wipe the pharynx and tonsils, or the secretions around the nasal cavity. One touch", quick and painless. Medical institutions will disinfect the inspection area, and medical staff will also disinfect their hands to avoid cross-infection. Generally speaking, the sampling process is safe and clean, without worrying about the risk of infection.
So which types of swab preservation solutions are divided into after sampling? There are two types of swab preservation solutions, inactivated and non-inactivated. At the beginning, almost all types of swab preservation solutions used in the market directly preserve live viruses. Inactivated swab preservation solution. This preservation method will cause the medical staff in sampling, transportation and testing to face a higher risk of infection. In response to this situation, the front line of anti-epidemics needs to store swabs that can directly inactivate the virus. However, it should be noted that while inactivating the virus, it should also be considered whether the preservation solution can stably preserve the integrity of the viral nucleic acid, so as to prevent the nucleic acid in the sample from degrading before the test, resulting in "false negative" nucleic acid testing. The production of the inactivated virus preservation solution developed to avoid this problem.
To put it simply, nucleic acid detection swab preservation solutions can be divided into two categories. The inactivated preservation solution developed by Desheng is a swab preservation solution with lysis function, which contains lysis salts for inactivating viruses and lysis proteins to protect nucleic acids. The virus preservation and lysis are completed in one step. The other is a non-inactivated type of preservation solution. Contrary to the inactivated type, it can protect the integrity of the virus protein and nucleic acid. In addition to nucleic acid detection, it can also be used for virus culture research. The operating environment requirements for this preservation solution are also different. The inactivated environment does not need to be so strict, while the non-inactivated type has higher operating environment requirements due to the risk of virus infection.
The inactivated preservation solution is a kind of virus preservation solution, which is mainly used to store the virus after the inactivation of the virus, and the inactivated virus preservation solution can effectively prevent the second infection of the user. Its characteristic is that it can quickly inactivate the virus, degrade the viral protein membrane capsid, and release the viral nucleic acid. At present, the new coronavirus that is sweeping the world in society is to release and degrade RNA viruses, so that post-reverse transcription PCR amplification experiments can be performed to achieve the purpose of using nucleic acid to detect viruses.
In addition to the lysing components, the inactivated virus preservation solution usually contains detergents that denature proteins and destroy the membrane structure, biological buffers, inhibitors that prevent nucleases from degrading nucleic acids, and reagents that maintain the stability of the nucleic acid structure. The lysis system may also add protease to cut the protein into small fragments to promote the separation of protein and nucleic acid.
At present, there are many known methods for lysing viruses. Two of the more common methods are heating method and concentrated salt method:
The concentrated salt method uses high concentration of salt to destroy the secondary bond between nucleic acid and protein, disassociating the nucleoprotein and releasing the viral nucleic acid. Usually, guanidine hydrochloride, guanidine isothiocyanate or non-guanidine salt cleavage salt can be used to directly lyse and inactivate the virus without heating the sample, which is relatively simple.
The heating method is based on heating to a temperature value of 80 C ~ 100 C, because generally samples are frozen at -70 C, generally need to be heated for 5 to 10 minutes, this operation is relatively simple, only one step can be used for The template for nucleic acid PCR amplification does not require any operation steps such as centrifugation and extraction, and is faster than the commonly used CheleX100 extraction method and phenol/chloroform methods. Although this method is simple, the amount of nucleic acid extracted will be relatively small.
Since the outbreak of the epidemic, Hubei Xindesheng Materials Co., Ltd. has been devoted to the research and development of virus preservation solutions, striving to provide society with its own strength. After many experiments and tests, Desheng has been able to ensure that the product quality and experience are very up to standard. Among them, our company produces inactivated and non-inactivated virus preservation solutions, which can be adjusted and selected on demand. In addition, Desheng reminds our customers that when purchasing virus preservation solutions, you should not blindly pursue low prices. You can take samples separately and go back for testing and comparison. The best is the best.
The vacuum blood collection device consists of three parts: vacuum blood collection tube, blood collection needle (including straight needle and scalp blood collection needle), and needle holder. Vacuum blood collection tube is the main component, mainly used for blood collection and storage. A certain amount of negative pressure is preset in the production process. When the blood collection needle punctures into the blood vessel, the blood automatically flows into the blood collection tube due to the negative pressure in the blood collection tube ; At the same time, various additives are preset in the blood collection tube, which can fully meet the clinical multiple comprehensive blood tests, safe, closed, and convenient for transportation.
Vacuum blood collection tubes are generally divided into the following types:
1. Dry empty tube without additives: The inner wall of the blood collection tube is evenly coated with the agent (silicone oil) to prevent wall hanging. It uses the principle of natural blood coagulation to coagulate the blood. After the serum is naturally precipitated, it is centrifuged for use. Mainly used for serum biochemistry (liver function, kidney function, myocardial enzymes, amylase, etc.), electrolytes (serum potassium, sodium, chloride, calcium, phosphorus, etc.), thyroid function, drug testing, AIDS testing, tumor markers, serum immunity learn.
2. Coagulation accelerator tube: the inner wall of the blood collection tube is evenly coated with silicone oil to prevent wall hanging, and coagulant is added. The coagulant can activate the fibrinase, so that the soluble fibrin becomes insoluble fibrin aggregates, and then forms a stable fibrin clot. If you want to get the result quickly, you can use the coagulation tube. Generally used in emergency biochemistry.
3. Blood collection tube containing separation gel and coagulant: The tube wall is siliconized and coated with coagulant to accelerate blood coagulation and shorten the inspection time. Separating glue is added in the tube. The separating glue has a good affinity with the PET tube, and it does play a role in isolation. Generally, even on ordinary centrifuges, the separating glue can remove the liquid components (serum) and solid components (blood cells) in the blood. Completely separate and accumulate in the test tube to form a barrier. After centrifugation, no oil droplets are produced in the serum, so the machine will not be blocked. Mainly used for serum biochemistry (liver function, kidney function, myocardial enzymes, amylase, etc.), electrolytes (serum potassium, sodium, chloride, calcium, phosphorus, etc.), thyroid function, drug testing, AIDS testing, tumor markers, serum immunity learn.
4. Blood collection tube containing anticoagulant in the tube
HEPES (4-hydroxyethyl piperazine ethanesulfonic acid) is a kind of hydrogen ion buffer with pH value ranging from 6.8 to 8.2. In the process of cell culture, we often need HEPES buffer. It is a kind of weak acid. In the open culture condition, if HEPES is added, it can prevent the increase of pH oxidation in the culture medium and maintain the pH at about 7.0. The final concentration of HEPES buffer was 10-50 mmol / L, which had no toxic effect on cells. Generally, 20 mmol / L HEPES can achieve buffering capacity. Since we often use it in cell culture medium, what are its advantages in cell culture medium?
Most of the cells need pH 7.2-7.4, but the optimal pH for cell culture varies with different cell types. Fibroblasts prefer higher pH (7.4-7.7), while the subculture and transformation cell lines need acid pH (7.0-7.4). HEPES is a kind of nonionic amphoteric buffer, which has good buffer capacity in the range of pH 7.2-7.4. The advantage of this method is that it can maintain a relatively constant pH value in open culture or cell observation. Under this condition, the cover of the cell culture bottle should be tightened to prevent a small amount of carbonate needed in the culture medium from scattering into the air.
HEPES produced by Desheng is white crystal powder, with main content of 99.50%, iron ion 5ppm, sulfate 0.05%, and heavy metal residue (PB) 10ppm. Can provide trial sample powder, self-made solution for testing.
Desheng Biochemical Technology Co., Ltd. was established in 2005. After years of innovation and development, the company has now formed a product led by "in vitro diagnostic reagent raw materials", "color reagents", "biological buffers" and other products, integrating R&D, production, Sales as one of the national high-tech enterprises. And it has established an independent research and development test center and production base, which can provide customers with high-quality research products commissioned and customized services and mass production services.
Desheng Biochemicalâ??s main product biological buffer is now the main series: TAP buffer, CAPS buffer, BICIEN buffer, tris buffer, etc. The application of biological buffers in the biochemical field is very extensive, and it will not participate. And the special characteristics of not interfering with the biochemical process, the first buffer system used in life science research, to provide a stable PH experimental environment for research. In addition, Desheng Biochemical actively promotes the promotion and use of biological buffers in more fields. It is currently widely used in in vitro diagnostics, biopharmaceuticals, biological cosmetics, coatings and paints and other industries, and has reached agreements with many Fortune 500 companies. Stable cooperative relationship.
Desheng biological buffer
The biological buffer system contains 20-30 varieties. Desheng Technology covers several of the most important products, which can meet the routine needs of major manufacturers, whether it is the HEPES biological buffer added to the inactivated virus preservation solution, there are To help increase the survival time and infection stability of the virus, it is the commonly used Tris in DNA electrophoresis buffer, or the most widely used CAPS in Western blotting. Not to mention Bicine, it is also widely used and can be used in enzyme reaction buffer. Electrophoresis buffer, used in the preparation of stable substrate solutions, is also commonly used in serum guaninase determination. It can be said that the application of biological buffers involves all aspects.
Material science needs precipitation. As a domestic company that independently develops biological buffers, Desheng has been focusing on R&D and innovation for more than ten years. With the vision of "serving for human health", it has continuously promoted the technological improvement and innovation of medical chemical materials. The field of buffers already possesses the technical strength to directly compete with international giants and has huge potential for domestic substitution. Desheng will further promote and consolidate the advantages of biological buffers in life sciences, in vitro diagnostics and other industries. Looking forward to Desheng achieving greater breakthroughs and development in the future.
Caps, also known as 3-cyclohexylaminopropanesulfonic acid, is a kind of biological buffer. Its appearance is white crystal or powder. Caps can be dissolved in water, pH buffer range is 8.9-10.3, pKa value is 9.6, molecular weight is 243.28, CAS number is 1135-40-6. Caps has a wide range of applications, including DNA / RNA extraction kit, PCR diagnostic kit, biochemical diagnostic kit, welding materials, air conditioning equipment, lithium metal, new coatings, new industrial materials, etc.
In the extraction of nucleic acid, the solution for nucleic acid hybridization can be prepared together with 3 - [(3-choleamidopropyl) dimethylammonium] - 1-propanesulfonate (chaps), 3 - (cyclohexylamino) - 2-hydroxy-1-propanesulfonate (capso), and 2 - (cyclohexylamino) ethanesulfonate (ches). It can reduce the yield of non-specific hybrids and improve the specificity of nucleic acid hybridization, while maintaining the yield of target hybridization products. It has important value in the identification of specific pathogens, diagnosis of genetic diseases, gene sequence analysis and so on.
Caps can be used in all kinds of environment-friendly high-quality waterborne two-component polyurethane coatings. Caps can coexist with active genes (hydroxyl, carboxyl, amine, epoxy) for a long time at room temperature. Caps is widely used in coatings market. In addition, caps can react with aliphatic polyisocyanate under mild conditions and in the presence of tertiary amine neutralizer. The sulfonated urea derivative is a very good emulsifier.
In biological experiments, there is a very important pH stabilizing reagent, which is the biological buffer caps. In the experiment, we usually choose the appropriate weak acid and its conjugated base to get the appropriate pH value. In a large number of experiments, it has been proved that most biological reactions occur under neutral conditions. Generally, the pH value is between 6-8, and the effective buffer range of buffer is between 6-8. Therefore, the acid-base form of buffer should not chelate with some metal ions.
Caps, also known as 3-cyclohexylaminopropanesulfonic acid, is a kind of biological buffer, which can be used for enzyme chemistry and HPLC separation of basic drugs. The appearance is white crystal or powder, caps can be dissolved in water, pH buffer range is 8.9-10.3, pKa value is 9.6, molecular weight is 243.28, CAS number is 1135-40-6 below 25 â??.
Desheng bio has been developing and producing biological buffer for many years, and has rich experience. Caps is the core product, which is highly praised in the field of biochemical diagnostic reagents and the market of new paint products. Desheng has been established for decades. The company's products are sold to more than 100 countries around the world. The product quality is good and the price is more favorable. If you are interested, you can call us. Desheng biology welcomes your call.
Climbazole, CAS number: 38083-17-9 English name: Climbazole, divided into solid and liquid forms, liquid Climbazole BP-8091 is a new generation of anti-dandruff and antipruritic agent, is a derivative of solid Climbazole, made of 1-p-chlorobenzene Synthesis of oxy-1-(N-imidazolyl)-1-(5-isothiazolone)-3, 3-dimethyl-2-butanone, stable in acidic and slightly alkaline systems, it is a High-efficiency broad-spectrum fungicide can inhibit a variety of fungi, especially for the fungus Drosophila ovalis, which produces human dander. It has the advantages of low irritation to the scalp, no need for heating, and convenient use. It is widely used in shampoos, shampoos, shower gels, foot baths, etc.
As a second-generation anti-dandruff agent, Ganbaosu has the advantages of good effect, safe use and good dissolving performance. It fundamentally blocks the channels of dandruff. Long-term use will not adversely affect the hair, and the hair after washing is loose and comfortable. sense. Desheng Chemical is specialized in providing raw materials and technical support for Ganbaosu and other daily chemicals.
Ganbaosu has a strong inhibitory effect on fungi that produce dandruff and has a good effect. It is soluble in surfactants, easy to use, no worries of stratification, stable to metal ions, non-yellowing, and discoloring. Ganbaosu has a variety of anti-fungal properties, especially for the main fungus that produces human dandruff-Bacillus ovatus, and also has obvious inhibitory effects on Candida albicans that cause oral gingivitis and periodontitis in humans. Therefore, it is widely used in washing products such as shampoo, shower gel, toilet soap, medicated soap, and medicine toothpaste, and has become an important functional additive in the daily chemical industry.
The Ganbaosu developed and produced by Desheng has been tested by the authoritative department and its quality indicators and safety performance indicators are in compliance with the standard requirements. Used by users, it has excellent performance such as high quality, low price, safety, good compatibility, and obvious anti-dandruff and anti-itching effects. The shampoo formulated with it will not cause precipitation, delamination, discoloration, skin irritation and other disadvantages. The preferred anti-itch and anti-dandruff agent for mid-to-high-end shampoos is very popular among users.
Buffer solution is a type of buffer system dedicated to life science research. In biochemical experiments, buffer solution plays an indispensable role. It can resist the influence of a small amount of strong acid and alkali from the outside, and maintain the system closest to the physiological environment. pH value. Both HEPES and PIPES buffers are commonly used buffers in biological experiments.
The pH buffer range of HEPES is 6.8-8.2. It is a zwitterionic biological buffer. It is soluble in water and does not form stable complexes with metal ions. In most cases, it will not interfere with biochemical processes and can be widely used in a variety of Biochemical reactions and used as buffer reagents in certain cell culture media. HEPES is often used in the cell culture medium of various types of organisms; in protein research, PIPES is often used as the component and eluent of the binding buffer in cation exchange chromatography; in DNA research, PIPES is used as calcium phosphate and DNA The buffer for the precipitation formation system, AFM and the buffer for electroporation experiments. In addition, HEPES interferes with the reaction between DNA and restriction enzymes, and is not suitable for Lowry's method to determine protein content. HEPES buffer is often used in the research of organelles and highly variable, pH-sensitive proteins and enzymes, as well as in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. It is a hydrogen ion buffer that can control a constant pH range for a long time. The final concentration is 10-50mmol/L, and the buffering capacity can be achieved when the culture medium contains 20mmol/L HEPES.
The pH buffer range of PIPES is 6.1-7.5, insoluble in water, soluble in NaOH aqueous solution. PIPES is different from buffers containing two (2-hydroxyethyl) amino groups (such as Bis-tris, Bicine), and can not form stable complexes with most metal ions. It is suitable for buffers in solution systems containing metal ions. According to existing research results, PIPES can be used to purify tubulin using phosphocellulose chromatography, to purify recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and to use as a buffer to crystallize transketolase from E. coli. In addition, because PIPES can form free radicals, it is not suitable for redox systems. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because PIPES has a relatively large ionic strength and its pKa value is concentration-dependent.
We must learn to distinguish between the similarities and differences in these reagents, so as to better promote the development and production of our different products. Desheng unanimously adheres to this kind of subtle difference, so that it can continue to develop and produce better products.
With the recurrence of asymptomatic, nucleic acid testing cannot be stopped for a moment. This is not only a protection for everyone, but also a protection for yourself. Speaking of nucleic acid testing, we have to bring up our virus sampling tube again. Its choice also plays a key role.
Why use a virus sampling tube?
Virus detection is different from conventional biochemical detection. The virus itself is a simple microorganism that must be parasitic in living cells. After sampling, the virus leaves the host cell, and its protein shell and nucleic acid will be quickly degraded in the sampling tube, so the nucleic acid During the test, it is impossible to determine whether the initially collected sample contains the virus, and it is easy to cause false negatives.
What are the requirements for an excellent virus sampling tube?
1. In terms of sample effectiveness: The non-inactivated virus preservation solution must maintain the activity of the pathogen's infectious agent. Preferably, it can preserve the activity of the virus at room temperature. The inactivated virus preservation solution needs to inactivate the virus but maintain the nucleic acid of the virus to meet the time from sample sampling to laboratory testing. It is necessary to limit and prevent the reproduction of symbiotic microorganisms to ensure the reliability of diagnostic tests.
2. In terms of safety: Because the virus sampling tubes are basically all infectious substances, and some are highly pathogenic infectious substances, the requirements for packaging containers are very strict, and they need to meet safety and ensure that liquids do not leak during transportation. .
So what is the virus preservation solution? Under what circumstances do I need to use a virus preservation solution?
The virus preservation solution is a protective liquid medium added to the virus sampling tube to protect the sample after the nasopharyngeal swab is sampled. Normally, nucleic acid PCR cannot be directly performed at the sample collection site during nucleic acid detection. If the sample collected by the swab needs to be transferred and transported, it is necessary to add a virus preservation solution.
Why is the virus preservation solution divided into inactivated and non-inactivated?
After the virus samples are collected, there is usually no way to test in time at the sample sampling site, so the collected virus swab samples need to be transported, and the virus itself will be quickly lysed outside the body and affect subsequent testing, so when storing and transporting , You need to add a virus preservation solution. For different detection purposes, you need to use different virus preservation solutions and different virus detection experimental conditions, so it is divided into two types of preservation solutions, inactivated and non-inactivated.
There are no other microorganisms, causing the virus to decompose after sampling or other influences causing false detections.
When the epidemic is waiting for an opportunity again, to quickly control the spread of the virus, nucleic acid testing has become the only way to go, and it has also attracted attention from all walks of life. However, there are many factors that affect the results of nucleic acid testing, including sample collection location, collection method, storage and transportation process, nucleic acid extraction operation, and so on. Among them, the stability of the sample is a key link. How to quickly inactivate the virus and protect the viral nucleic acid from being degraded, maintaining the stability of the virus sample storage solution has become a top priority.
Step 1: Break the shell and eliminate the "combat power"
Like other RNA viruses, the results of the new coronavirus are simply composed of a protein shell wrapped in single-stranded nucleic acid-RNA. We can simply think of the RNA of the virus as the "brain" that gives orders, and the protein is the "limbs" that execute orders. The new coronavirus infects the human body and reproduces and causes symptoms. This process is mainly completed by the biologically active protein structure outside the virus.
The inactivated virus preservation solution is mainly the virus modified by the nucleic acid extraction lysis solution; it is a powerful protein denaturant that can quickly dissolve the protein and cause the virus structure to be broken. When the clinical sample with the new coronavirus is mixed with the preservation solution, the virus in the sample is affected by the lysis solution, and the protein shell is quickly destroyed, and this damage is irreversible. At this time, the virus has lost its evil "minions" and no longer has the ability to infect people, thus ensuring the safety of contacts during sample transportation and processing.
Step 2: Inhibit enzyme activity and escort the "ID card"
After the shell is lysed, the RNA of the new coronavirus is free in the preservation solution. As a single-stranded macromolecule, the stability of RNA is not as immobile as double-stranded DNA. The RNase that exists everywhere in the natural environment is the culprit that causes RNA to hydrolyze and break. Therefore, it is generally considered that the viral RNA that has lost its outer shell protection is
In addition to the inactivated virus storage solution, the virus preservation solution produced by Desheng also has a non-inactivated virus storage solution. It retains the protein coat of the virus and the viral nucleic acid DNA or RNA at the same time, so that the virus has the integrity of the protein epitope and nucleic acid in vitro. Of course, there is a certain risk of infectivity when operating errors. Long-term storage after sampling needs to keep strictly low temperature. Regardless of the virus preservation solution, we need to strictly operate, store or transport in accordance with the requirements when using it.
