Swab Virus Preservation Solution

With the repeated reversal of the epidemic, the fight against the epidemic has become our primary concern for human beings. We must not only prevent the spread of the epidemic, but also protect our own safety, especially the staff who collect virus specimens. If the virus is not collected properly, it is very likely to be infected. Therefore, under such severe tests, the emergence of disposable virus storage tubes has greatly reduced the risk of infection and guaranteed the safety of our frontline personnel. Specimen collection method 1. Pharyngeal swab: Use two plastic rod swabs with polypropylene fiber heads to wipe both pharyngeal tonsils and posterior pharyngeal wall at the same time, and immerse the swab head in 3ml virus preservation solution (isotonic saline solution, tissue culture can also be used) Or phosphate buffer), discard the tail, and screw the cap tightly. 2. Nasal swab: Gently insert a plastic rod swab with a polypropylene fiber head into the nasal palate in the nasal passage, stay for a while and then slowly turn it out. Take another plastic rod swab with polypropylene fiber head and collect the other nostril in the same way. The two swabs mentioned above were immersed in the same tube containing 3ml sampling solution, the tail was discarded, and the tube cap was screwed tightly. 3. Use an oily marker on the PE tube label to indicate the type of sample, sampling time, number, and patient name. 4. After collection, put the disposable virus storage tube in the special transport box (or vaccine transport box), put it in the ice tray, fill it with soft material, and seal it, and it will be transported by a special person instead of mailing. It is best to use a special vehicle. 5. If the collected specimens cannot be delivered within 24 hours, they should be stored below -70�°C as soon as possible. If there is no condition of -70â??, it should be temporarily stored in the refrigerator at 4â?? for a short time, and the specimen should be contacted as soon as possible. 6. Influenza virus is unstable at -20�°C to -40�°C, so long-term storage is best performed below -70C. In this case, it greatly protects us, and it also protects the virus from degrading and causing false negatives. One-time virus preservation tube is a hero to defend in the epidemic.

What is nucleic acid detection, that is, after collecting human secretions, under laboratory conditions, by analyzing the RNA gene sequence of the virus, using PCR amplification method for detection, and diagnosis of clinical etiology. At present, nucleic acid testing is the most effective testing method for early determination of whether a patient is infected with the virus, early detection and early treatment. Nucleic acid detection swabs are divided into two types: nasal swabs and throat swabs. During the sampling process, the doctor will use a swab like a cotton swab to wipe the pharynx and tonsils, or the secretions around the nasal cavity. One touch", quick and painless. Medical institutions will disinfect the inspection area, and medical staff will also disinfect their hands to avoid cross-infection. Generally speaking, the sampling process is safe and clean, without worrying about the risk of infection. So which types of swab preservation solutions are divided into after sampling? There are two types of swab preservation solutions, inactivated and non-inactivated. At the beginning, almost all types of swab preservation solutions used in the market directly preserve live viruses. Inactivated swab preservation solution. This preservation method will cause the medical staff in sampling, transportation and testing to face a higher risk of infection. In response to this situation, the front line of anti-epidemics needs to store swabs that can directly inactivate the virus. However, it should be noted that while inactivating the virus, it should also be considered whether the preservation solution can stably preserve the integrity of the viral nucleic acid, so as to prevent the nucleic acid in the sample from degrading before the test, resulting in "false negative" nucleic acid testing. The production of the inactivated virus preservation solution developed to avoid this problem. To put it simply, nucleic acid detection swab preservation solutions can be divided into two categories. The inactivated preservation solution developed by Desheng is a swab preservation solution with lysis function, which contains lysis salts for inactivating viruses and lysis proteins to protect nucleic acids. The virus preservation and lysis are completed in one step. The other is a non-inactivated type of preservation solution. Contrary to the inactivated type, it can protect the integrity of the virus protein and nucleic acid. In addition to nucleic acid detection, it can also be used for virus culture research. The operating environment requirements for this preservation solution are also different. The inactivated environment does not need to be so strict, while the non-inactivated type has higher operating environment requirements due to the risk of virus infection.

VTM's - Virus Collection & Preservation System Used for universal virus collection and storage in a short time. Class 1

The sampling swab uses advanced jet implanted nylon fuzz technology. Compared with conventional fiber swabs, the sample is easily penetrated, diluted, and trapped in the fiber. The nylon flocking swab has outstanding sample collection and release capability, and the release rate can reach 90%, which is much higher than that of the conventional fiber swab. Light red media formation contains antibiotics that can effectively inhibit the reproduction of bacteria and fungi.

The inactivated preservation solution is a kind of virus preservation solution, which is mainly used to store the virus after the inactivation of the virus, and the inactivated virus preservation solution can effectively prevent the second infection of the user. Its characteristic is that it can quickly inactivate the virus, degrade the viral protein membrane capsid, and release the viral nucleic acid. At present, the new coronavirus that is sweeping the world in society is to release and degrade RNA viruses, so that post-reverse transcription PCR amplification experiments can be performed to achieve the purpose of using nucleic acid to detect viruses. In addition to the lysing components, the inactivated virus preservation solution usually contains detergents that denature proteins and destroy the membrane structure, biological buffers, inhibitors that prevent nucleases from degrading nucleic acids, and reagents that maintain the stability of the nucleic acid structure. The lysis system may also add protease to cut the protein into small fragments to promote the separation of protein and nucleic acid. At present, there are many known methods for lysing viruses. Two of the more common methods are heating method and concentrated salt method: The concentrated salt method uses high concentration of salt to destroy the secondary bond between nucleic acid and protein, disassociating the nucleoprotein and releasing the viral nucleic acid. Usually, guanidine hydrochloride, guanidine isothiocyanate or non-guanidine salt cleavage salt can be used to directly lyse and inactivate the virus without heating the sample, which is relatively simple. The heating method is based on heating to a temperature value of 80 C ~ 100 C, because generally samples are frozen at -70 C, generally need to be heated for 5 to 10 minutes, this operation is relatively simple, only one step can be used for The template for nucleic acid PCR amplification does not require any operation steps such as centrifugation and extraction, and is faster than the commonly used CheleX100 extraction method and phenol/chloroform methods. Although this method is simple, the amount of nucleic acid extracted will be relatively small. Since the outbreak of the epidemic, Hubei Xindesheng Materials Co., Ltd. has been devoted to the research and development of virus preservation solutions, striving to provide society with its own strength. After many experiments and tests, Desheng has been able to ensure that the product quality and experience are very up to standard. Among them, our company produces inactivated and non-inactivated virus preservation solutions, which can be adjusted and selected on demand. In addition, Desheng reminds our customers that when purchasing virus preservation solutions, you should not blindly pursue low prices. You can take samples separately and go back for testing and comparison. The best is the best.