The structure and function of the protein need to be maintained in a suitable pH environment. Therefore, the choice of pH buffer is very important during the preparation and purification of the protein. Only a suitable buffer can ensure the activity and structural stability of the protein.
Effect of buffer in protein purification process
There are many kinds of buffers for protein purification. While maintaining the pH of the system, it is necessary to ensure that the protein cannot be inactivated during the purification process, or to minimize the amount of protein inactivation, which must be maintained in every step of protein purification. Solubility and activity of protein.
Protein purification buffer requirements
The buffer in the protein purification process should not only prevent protein degradation, but also prevent protein aggregation, which has a significant impact on the experimental results. The following factors should be considered when selecting and reagent buffers: pH, buffer system, salt ions, reducing agents and stabilizers. Each of these factors should be optimized according to the purified target protein, and the activity of the target protein should be used as an evaluation criterion for optimization.
Understand protein stability conditions
There are many types of proteins, and different proteins adapt to different pH values. The buffer should be selected according to its optimal pH value. In many cases, the pH is 7.4, which is a mimic biological condition, and most proteins are stable at this pH. However, there are some exceptions, which require pH adjustment to keep the protein soluble and not degraded.
Commonly used buffers for protein purification
Commonly used buffers in the protein purification process include Tris, Tricine, Bis-Tris, MOPS, etc. Among them, Tris is commonly used. Its buffer range is close to physiological pH and has a low ionic strength. It is a good solvent for proteins. Can ensure the stability of the protein. It should be noted that when preparing the buffer, try to avoid the isoelectric point pl of the target protein. Because the protein has no electrostatic charge on its surface in the pH solution of its isoelectric point, the repulsive force between the hundred molecules is reduced, it is easy to aggregate, and the solubility is reduced.
Now the adaptation pH value, isoelectric point, and buffer range of the buffer to be purified can be found, and you can directly match it according to the corresponding requirements. Desheng is a manufacturer of in vitro diagnostic reagents, which can provide large-package pH buffers such as Tris, MOPS, Bicine, etc.
Applications: In Animal feed stuff Nutrition feed for Poultry, Layers, Cattle. Primarily used as a dairy cow feed supplement, the buffering capability of Natural Soda's pure and natural feed grade sodium bicarbonate helps stabilize rumen pH by reducing acidic conditions. Improves calcium absorption and eggshell quality of laying hens during peak production.
Product Name: Sodium permanganate Use level: Industrial Content: 40 (%) CAS Number: 10101-50-5 Molecular formula:NaMnO4 Molecular Weight: 141.93 Appearance: purple liquid. A density of 1.36 ~ 1.39 g / cm3, soluble in water, ethanol and ether, soluble in liquid ammonia. Decomposition in alkali. Strong oxidizing. And chemical properties similar to potassium permanganate. Purposes: as oxidants, disinfectants, fungicides and phosphorous antidotes. Toluene is also used to manufacture saccharin, o-toluene sulfonamide and organic synthesis and purification of phenol wastewater treatment. Can be used for circuit boards, metal surface cleaning, the conductive film cleaning to remove grease, electrolytic manganese dioxide, chemical fiber finishing, electroplating addition to hydrogen sulfide and other odors, as well as water treatment and so on. Packaging and storage: Industrial Pails barrel, net weight 25kg plastic drum, 250kg steel-plastic composite barrels, 1250kg IBC barrels packaging. Store in a cool dry place. Quality Standard: Â Items index Sodium permanganate (NaMnO4) Content / (%) 40 Water-insoluble content / (%) 0.01 Potassium (K) content / (%) 0.22 pH value 6 ?8 Density / (g/cm3) 1.36 1.39 Chloride (Cl) content / (%) 0.02
1. Chemical Name: Sodium Pyrophosphate 2. Formula: Na4P2O7, Na4P2O7, 10H2O 3. Molecular weight: 265.90, 446.06 4. Specificity: Colorless or white crystals, crystalline or granular powder. It is easily soluble in water, but is insoluble in alcohol. Density: 2.45, relative density: 2.45, melting point: 890? . The decahydrate effloresces slightly in dry air. pH of 1% solution is about 10. 5. Quality Standard: 6. Usage: It is mainly used as detergent, electroplating . ctFor food grade it is mainly used as emulsifier, buffer, nutrient, sequestrant, texturizer 7. Packing: 25kgs net paper or plastic woven bags. 8. Storage and Transport: It should be stored in a dry, ventilative and clean warehouse kept away from moisture and hot, Furthermore, it should be stored separately from poisonous Substances.
Main Information: Sodium Sesquicarbonate Molecular Formula: Na2CO3.NaHCO3.2H2O CAS Number: 533-96-0 EC Number: 205-580-9 Manufacturer: TATA Chemicals Europe Appearance: Granular Also Known As: Trisodium Hydrogendicarbonate Sesqui CREX Key Properties: Sodium Sesquicarbonate (Borax Substitute) is a unique alkaline product that is ideal for a wide range of household, personal care and industrial uses. Manufactured by Tata Chemicals Europe at its dedicated plant in the UK, Sodium Sesquicarbonate (Borax Substitute)is a free-flwing, non-irritant white powder. It is produced by the recrystallisation of Sodium Carbonate and Sodium Bicarbonate, to form a single crystal of Sodium Sesquicarbonate (Borax Substitute)Na2CO3.NaHCO3.2H2O. Sodium Sesquicarbonate (Borax Substitute)avoids pH surges seen with blends of Sodium Bicarbonate and Sodium Carbonate due to their different dissolution rates. The varied and valuable properties of Sodium Sesquicarbonate (Borax Substitute), such as water softening, high solubility, mild alkalinity, free flow and excellent buffering capacity, lead to uses in a wide range of applications.
TetraSodium Pyrophosphate, E450, CAS no.7722-88-5, white powder or granular, also called sodium pyrophosphate, tetrasodium phosphate or TSPP, manufacturing process from Disodium Phosphate. Tetrasodium pyrophosphate is used as a buffering agent, an emulsifier, a dispersing agent, and a thickening agent, and is often used as a food additive. TSPP is used to modify the proteins enabling them to retain moisture during storage, thawing, and cooking. As an experienced TetraSodium Pyrophosphate manufacturer and supplier, we has been supplying and exporting TetraSodium Pyrophosphate for almost 10 years, please be assured to buy from us. Any inquiries about price and the market trend please feel free to contact us, we will reply you within 1 working day. TetraSodium Pyrophosphate Specification ITEM STANDARD NaH2PO7 >95% PH at 1% 9.9-10.7 Loss of Ignition
Disodium Pyrophosphate,E541, CAS no.7758-16-9, is a popular leavening agent used in baking powders. available as WHITE POWDER. Disodium Pyrophosphate is widely used as food buffering and chelating agent in food processing. As an experienced Disodium Pyrophosphate manufacturer and supplier, we has been supplying and exporting Disodium Pyrophosphate for almost 10 years, please be assured to buy from us. Any inquiries about price and the market trend please feel free to contact us, we will reply you within 1 working day. Disodium Pyrophosphate Specification ITEM STANDARD Na2H2P2O7 95.0% MIN APPEARANCE WHITE POWDER WATER INSOLUBLE 1.0% MAX ARSENIC( AS) 3PPM MAX LOSS ON DRYING 0.5% MAX CADMIUM (PPM) 1 MAX LEAD (PPM) 4 MAX MERCURY (PPM) 1 MAX HEAVY METAL( PB) 15PPM MAX FLUORID( PPM) 10 MAX PH VALUE 3.5-4.5
Tetrapotassium Pyrophosphate (TKPP) E450, CAS no.7320-34-5, white powder, a potassium salt of phosphoric acid manufactured from Dipotassium Phosphate. Tetrapotassium Pyrophosphate is used as buffering agent, emulsifier, dispersing agent and thickening agent in food production. As an experienced Tetrapotassium Pyrophosphate manufacturer and supplier, we has been supplying and exporting Tetrapotassium Pyrophosphate for almost 10 years, please be assured to buy from us. Any inquiries about price and the market trend please feel free to contact us, we will reply you within 1 working day. Tetrapotassium Pyrophosphate Specification ITEM STANDARD ASSAY K4P2O7) 98.0% MIN APPEARANCE WHITE POWDER WATER SOLUBLE 0.2% MAX PH 9.9-10.7 ARSENIC( AS) 3PPM MAX LOSS ON IGNITION 2.0% MAX Pb (PPM) 5 MAX HEAVY METAL( PB) 15 PPM MAX ASRENIC(AS) 3 PPM MAX FLUORID( PPM) 10 MAX
Sodium Diacetate, E262,CAS no.126-96-5, Sodium Diacetate is a white crystalline powder with an acetic acid odor. Functions: Inhibits growth of micro-organisms; Aroma/flavour enhancing; pH control agent. As a buffering agent, sodium diacetate is used in meat products to control their acidity. As a food preservative, sodium diacetate used to inhibit the growth of various microorganisms in meat products, thus it can be used as a preservative and safeguard for food safety and shelf life extension. As an experienced Sodium Diacetate manufacturer and supplier, we has been supplying and exporting Sodium Diacetate for almost 10 years, please be assured to buy from us. Any inquiries about price and the market trend please feel free to contact us, we will reply you within 1 working day. Sodium Diacetate powder Specification Item Specification Appearance White, hygroscopic crystalline solid with an acetic odour Free Acetic Acid (%) 39.0- 41.0 Sodium Acetate (%) 58.0- 60.0 Moisture (Karl Fischer method, %) 2.0 Max pH (10% Solution) 4.5- 5.0 Formic acid, formates andother oxidizable (as formic acid) 1000 mg/ kg Particle Size Min 80% Pass 60 mesh Arsenic (As) 3 mg/ kg Lead (Pb) 5 mg/ kg Mercury (Hg) 1 mg/ kg Heavy Metal (as Pb) 0.001% Max
Monoammonium Phosphate, E342, CAS no.7722-76-1, also known as Ammonium dihydrogen phosphate, a salt of phosphoric acid manufactured through chemical synthesis, ammonia and phosphoric acid as raw materials. Properties: white crystalline powder, soluble in water. Monoammonium Phosphate (Food Grade) is used in food as a pH control agent, buffering agent, acidulate, and nutrient source. Also in bread and dough, dry powder beverages, eggs, isotonic beverages, mineral supplements, starter cultures and yeast cultures. As an experienced Monoammonium Phosphate manufacturer and supplier, we has been supplying and exporting Monoammonium Phosphate for almost 10 years, please be assured to buy from us. Any inquiries about price and the market trend please feel free to contact us, we will reply you within 1 working day. Monoammonium Phosphate Specification No. standard name industrial grade food grade 1 Main content(as NH4H2PO4)% 99.0 99.0 2 Phosphorus pent-oxide(as P2O5)% 61.0 61.0 3 Nitrogen(as N)% 11.8 11.8 4 PH value 4.4-4.8 4.4-4.8 5 Moisture% 0.2 0.2 6 Heavy metal(as Pb)% 0.001 7 Arsenic(As)% 0.0003 8 Fluoride(as F)% 0.005 9 Water insoluble % 0.10 0.10
