Buffer solution is a type of buffer system dedicated to life science research. In biochemical experiments, buffer solution plays an indispensable role. It can resist the influence of a small amount of strong acid and alkali from the outside, and maintain the system closest to the physiological environment. pH value. Both HEPES and PIPES buffers are commonly used buffers in biological experiments.
The pH buffer range of HEPES is 6.8-8.2. It is a zwitterionic biological buffer. It is soluble in water and does not form stable complexes with metal ions. In most cases, it will not interfere with biochemical processes and can be widely used in a variety of Biochemical reactions and used as buffer reagents in certain cell culture media. HEPES is often used in the cell culture medium of various types of organisms; in protein research, PIPES is often used as the component and eluent of the binding buffer in cation exchange chromatography; in DNA research, PIPES is used as calcium phosphate and DNA The buffer for the precipitation formation system, AFM and the buffer for electroporation experiments. In addition, HEPES interferes with the reaction between DNA and restriction enzymes, and is not suitable for Lowry's method to determine protein content. HEPES buffer is often used in the research of organelles and highly variable, pH-sensitive proteins and enzymes, as well as in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. It is a hydrogen ion buffer that can control a constant pH range for a long time. The final concentration is 10-50mmol/L, and the buffering capacity can be achieved when the culture medium contains 20mmol/L HEPES.
The pH buffer range of PIPES is 6.1-7.5, insoluble in water, soluble in NaOH aqueous solution. PIPES is different from buffers containing two (2-hydroxyethyl) amino groups (such as Bis-tris, Bicine), and can not form stable complexes with most metal ions. It is suitable for buffers in solution systems containing metal ions. According to existing research results, PIPES can be used to purify tubulin using phosphocellulose chromatography, to purify recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and to use as a buffer to crystallize transketolase from E. coli. In addition, because PIPES can form free radicals, it is not suitable for redox systems. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because PIPES has a relatively large ionic strength and its pKa value is concentration-dependent.
We must learn to distinguish between the similarities and differences in these reagents, so as to better promote the development and production of our different products. Desheng unanimously adheres to this kind of subtle difference, so that it can continue to develop and produce better products.
Supplier: Pipes, pipe fittings, flanges, fasteners, round bars, sheets, plates, wires, wire mesh, valves, buttweld fittings, socketweld fittings, etc all in the grades of stainless steel, carbon steel, alloy steel, titanium, monel, inconel, incoloy, nickel, hastelloy, zirconium, hastalloy, invar, duplex, super duplex, mild steel, etc
GI Conduit is a pipe used to protect and route electrical wiring in a building or construction. It provides very good protection to enclosed conductors from impact, moisture, and chemical vapors. Specifications We can Supply ERW Stove Enameled Rigid Steel Conduit pipes ( Medium protection) as per ISI 9537, Part II/ 1981 and Hot Dipped Galvanized Rigid Steel Conduit pipes ( High Protection) as per ISI 9537, Part II/ 1981. Size in outer diameter : 20mm, 25 mm, 32mm, 40mm,50mm Wall Thickness : 16 SWG(1.40-1.80MM) and 1.40 SWG(1.60-2.20 MM)
GI Conduit is a pipe used to protect and route electrical wiring in a building or construction. It provides very good protection to enclosed conductors from impact, moisture, and chemical vapors. Specifications We can Supply ERW Stove Enameled Rigid Steel Conduit pipes ( Medium protection) as per ISI 9537, Part II/ 1981 and Hot Dipped Galvanized Rigid Steel Conduit pipes ( High Protection) as per ISI 9537, Part II/ 1981. Size in outer diameter 20mm, 25 mm, 32mm, 40mm,50mm Wall Thickness 16 SWG(1.40-1.80MM) and 1.40 SWG(1.60-2.20 MM)
Inconel 601, an alloy material. Excellent oxidation resistance at high temperature, good carbonation resistance, good oxidation resistance to sulfur-containing atmosphere, good mechanical properties at room temperature and high temperature, good resistance to stress corrosion cracking due to controlled Carbon content and grain size, 601 has higher creep rupture strength, so it is recommended to use 601 in the field above 500 �°C. Below are the international standard designations and specifications for Inconel 601: American standard designation: UNS N06601, Specifications: ASTM B166, ASTM B167, ASTM B168, ASTM B775, ASTM B829, ASTM B517, ASTM B751, ASTM B775, ASTM B829, ASTM B775, ASTM B751, ASTM B775, AMS 5715, AMS 5870, AMS 5871, AMS 5580, AMS 5715, AMS 5870, AMS 5871, AMS 5580. German standard designation: W.Nr. 2.4851, Specifications: DIN 17742, DIN 17750, DIN 17751, DIN 17752, DIN 17753, DIN 17754. Japanese standard designation: JIS NCF 601, Specifications: JIS G4902, JIS G4903, JIS G4904, JIS G4905, JIS G4906, JIS G4907. Chinese standard designation: GH236, Specifications: GB/T 5580, GB/T 14992, GB/T 14995, GB/T 14997, GB/T 15000, GB/T 15001. We can provide the following the form:Inconel601 alloy coil; Inconel601 alloy strip;Inconel601 alloy plate; Inconel601 alloy sheets; Inconel601 alloy tube; Inconel601 alloy pipe; Inconel601 alloy flat; Inconel601 alloy bar GB:1Cr23Ni60Fe13Al;JIS:NAS 601/NCF 601;UNS:N06601;W.Nr:2.4851 1. Trays, baskets and fixtures used in heat treatment factories. 2. Steel wire split annealing and radiant tubes, high-velocity gas burners, wire mesh belts in industrial furnaces. 3. Isolation tanks in ammonia reforming and catalytic support grids in nitric acid manufacturing. 4. Exhaust system components 5. Combustion chamber of solid waste incinerator 6. Pipe supports and soot handling components 7. Exhaust gas detoxification system components 8. Oxygen reheater
Bhagyalaxmi Industrial is globally acclaimed as a reliable manufacturer supplier and exporter of SS 304 Pipes We provide a comprehensive range of SS 304L Pipes in different dimensions and specifications Also we are capable enough of offering the superior quality of Pipes and Tubes in customized specifications Moreover we bring our pipes and tubes as per the latest industry trends to serve with the latest featuresAlloy stainless steel grade 304 is the austenitic stainless steel part of the T300 SS series The alloy is a mixture of Chromium and Nickel along with the addition of elements This alloy is also called nickel and chromium austenitic alloy SS 304 is less electrically and thermally conductive than carbon steel It is essentially nonmagnetic SS 304 Tubes has higher corrosion resistance than regular steel and is widely used because of the ease with which it is formed into various shapes The SS 304L has many good welding and forming attributes
444 stainless steel is a low-carbon, dual-stabilized ferritic stainless steel that is highly resistant to corrosion, especially in chloride environments. It is often used in applications where high corrosion resistance and good formability are required. Some of the key properties of 444 stainless steel include: Corrosion resistance: 444 stainless steel has excellent resistance to corrosion, especially in chloride environments such as seawater, brackish water, and chloride-containing acids and solvents. Heat resistance: It can withstand high temperatures up to 950�°C (1742�°F) without losing its strength or corrosion resistance properties. Formability: It has good formability and can be easily fabricated into various shapes and sizes. Weldability: It can be welded using common welding techniques, and does not require preheating or post-weld heat treatment. Cost-effective: Compared to other stainless steels, 444 stainless steel is a cost-effective option due to its lower nickel content. Some of the common applications for 444 stainless steel include: Automotive exhaust systems: It is often used in the manufacture of automotive exhaust systems due to its excellent corrosion resistance and heat resistance. Industrial equipment: It is also used in various industrial equipment such as heat exchangers, tanks, and piping systems. Kitchen appliances: It is used in the manufacture of kitchen appliances such as refrigerators, dishwashers, and ovens due to its good formability and corrosion resistance. Overall, 444 stainless steel is a highly versatile and cost-effective material that is ideal for use in corrosive environments where high corrosion resistance and good formability are required. Here are some of the most common international standards for 444 stainless steel: ASTM A240/A240M: Standard Specification for Chromium and Chromium-Nickel Stainless Steel Plate, Sheet, and Strip for Pressure Vessels and for General Applications ASTM A268/A268M: Standard Specification for Seamless and Welded Ferritic and Martensitic Stainless Steel Tubing for General Service ASTM A554: Standard Specification for Welded Stainless Steel Mechanical Tubing EN 10088-2: Stainless steels - Part 2: Technical delivery conditions for sheet/plate and strip of corrosion resisting steels for general purposes JIS G4305: Cold-rolled stainless steel plate, sheet and strip GB/T 3280: Cold rolled stainless steel plate, sheet and strip We can provide the following of the form:444 stainless steel coil; 444 stainless stee strip;444 stainless stee plate; 444 stainless steel sheets; 444 stainless steel tubes; 444 stainless steel pipe;444 stainless steel flat; 444 stainless steel bar 444 stainless steel is generally used to manufacture stress corrosion resistant equipment, video machinery, automotive engine exhaust pipe storage tanks, solar water heaters, printing and dyeing machinery, heat exchangers, etc.
321 stainless steel is an austenitic stainless steel that contains titanium as a stabilizing element. This element helps to prevent chromium carbide precipitation, which can occur during high-temperature service, making it ideal for use in high-temperature applications. Some of the key properties of 321 stainless steel include: High-temperature resistance: 321 can withstand high temperatures up to 870�°C (1600�°F) without losing its strength or corrosion resistance properties, making it ideal for use in applications such as furnace parts and exhaust systems. Corrosion resistance: 321 offers excellent resistance to corrosion, including aqueous corrosion and stress corrosion cracking. Strength and toughness: 321 has good strength and toughness, making it suitable for use in demanding applications. Fabricability: 321 is easily formable using standard methods, and can be welded using common welding techniques. Some of the common applications for 321 stainless steel include: Exhaust systems in automobiles, aircraft, and industrial equipment High-temperature furnace parts, such as burners and heating elements Chemical processing equipment Jet engine parts Heat exchangers Overall, 321 stainless steel is a highly reliable and versatile material that is well-suited for use in high-temperature applications where superior corrosion resistance and strength are required. The following are some of the commonly used international standards for 321 stainless steel: ASTM A240/A240M: Standard Specification for Chromium and Chromium-Nickel Stainless Steel Plate, Sheet, and Strip for Pressure Vessels and for General Applications ASTM A276/A276M: Standard Specification for Stainless Steel Bars and Shapes EN 10088-2: Stainless steels - Part 2: Technical delivery conditions for sheet/plate and strip of corrosion resisting steels for general purposes EN 10095: Heat resisting steels and nickel alloys JIS G4304: Hot-rolled stainless steel plate, sheet and strip JIS G4305: Cold-rolled stainless steel plate, sheet and strip GB/T 4237: Hot rolled stainless steel plate, sheet and strip GB/T 3280: Cold rolled stainless steel plate, sheet and strip We can provide the following form:321 stainless steel coil; 321 stainless stee strip;321 stainless stee plate; 321 stainless steel sheets; 321 stainless steel tubes; 321 stainless steel pipe; 321 stainless steel flat; 321 stainless steel bar Applied to open-air machines, heat-resistant parts of building materials and parts with difficulty in heat treatment in chemical, coal and petroleum industries that require high resistance to intergranular corrosion 1. Petroleum waste gas combustion pipeline 2. Engine exhaust pipe 3. Boiler shell, heat exchanger, heating furnace parts 4. Silencer parts for diesel engines 5. Boiler pressure vessel 6. Chemical transport vehicle 7. Expansion joints 8. Spiral welded pipes for furnace pipes and dryers
2205 stainless steel, also known as duplex stainless steel, is a type of stainless steel that is characterized by its two-phase microstructure consisting of both austenitic and ferritic phases. This combination of phases provides the alloy with improved strength and corrosion resistance compared to standard austenitic stainless steels. Some of the key properties of 2205 stainless steel include: High strength: 2205 stainless steel has excellent strength properties, making it suitable for use in demanding applications such as structural components and pressure vessels. Corrosion resistance: 2205 stainless steel provides good corrosion resistance in a variety of environments, including chloride-containing and acidic environments. Weldability: 2205 stainless steel is easily weldable using common welding techniques and does not require preheating or post-weld heat treatment. Fatigue resistance: 2205 stainless steel has good fatigue resistance, which makes it suitable for use in applications where cyclic loading is present. High toughness: 2205 stainless steel exhibits high toughness even at low temperatures, making it suitable for use in cryogenic applications. Overall, 2205 stainless steel is a highly versatile material that offers excellent strength and corrosion resistance properties, making it ideal for use in a wide range of demanding applications. Here are some of the most common international standards for 2205 duplex stainless steel: ASTM A240/A240M: Standard Specification for Chromium and Chromium-Nickel Stainless Steel Plate, Sheet, and Strip for Pressure Vessels and for General Applications ASTM A789/A789M: Standard Specification for Seamless and Welded Ferritic/Austenitic Stainless Steel Tubing for General Service ASTM A790/A790M: Standard Specification for Seamless and Welded Ferritic/Austenitic Stainless Steel Pipe EN 10088-2: Stainless steels - Part 2: Technical delivery conditions for sheet/plate and strip of corrosion resisting steels for general purposes EN 10216-5: Seamless steel tubes for pressure purposes - Technical delivery conditions - Part 5: Stainless steel tubes NACE MR0175: Sulfide stress cracking-resistant metallic materials for oilfield equipment NORSOK MDS D45: Qualification of manufacturers of duplex stainless steels UNS S31803/S32205: Unified numbering system designations for duplex stainless steels We can provide the following form:2205 stainless steel coil; 2205 stainless stee strip;2205 stainless stee plate; 2205stainless steel sheets; 2205 stainless steel tubes; 2205stainless steel pipe;2205 stainless steel flat; 2205 stainless steel bar
420 stainless steel is a kind of martensitic stainless steel, which has the characteristics of certain wear resistance and corrosion resistance, and high hardness. 420 stainless steel is a martensitic grade of stainless steel that contains chromium and molybdenum, making it corrosion-resistant and hardenable. It has good strength, hardness, and wear resistance, and is commonly used in applications where high strength and moderate corrosion resistance are required, such as in cutlery, surgical instruments, and dental equipment. Some of the key properties of 420 stainless steel include: Corrosion resistance: 420 has good corrosion resistance in mild atmospheric environments and in most mild chemical environments. Hardness: 420 is a hardenable stainless steel and can be hardened by heat treatment, making it ideal for applications where high hardness is required. Wear resistance: 420 has good wear resistance due to its high hardness. Machinability: 420 has moderate machinability in the annealed condition, but is more difficult to machine in the hardened condition. Some of the common applications for 420 stainless steel include: Cutlery, including knives, scissors, and blades Surgical instruments, such as scalpels and forceps Dental equipment, including dental drills and hand tools Valve parts Pump shafts and bearings Overall, 420 stainless steel is a reliable and versatile material that is well-suited for use in applications where high strength, hardness, and moderate corrosion resistance are required. Here are some of the most common international standards for 420 stainless steel: ASTM A276: Standard Specification for Stainless Steel Bars and Shapes ASTM A314: Standard Specification for Stainless Steel Billets and Bars for Forging ASTM A473: Standard Specification for Stainless Steel Forgings ASTM A580: Standard Specification for Stainless Steel Wire EN 10088-3: Stainless steels - Part 3: Technical delivery conditions for semi-finished products, bars, rods, wire, sections and bright products of corrosion resisting steels for general purposes JIS G4303: Stainless steel bars GB/T 1220: Stainless steel bars We can provide the following form:420 stainless steel coil; 420 stainless stee strip;420 stainless stee plate; 420 stainless steel sheets; 420 stainless steel tubes; 420 stainless steel pipe; 420 stainless steel flat; 420 stainless steel bar 420 stainless steel is suitable for all kinds of precision machinery, bearings, electrical appliances, equipment, instruments, meters, transportation tools, household appliances, etc. It is mostly used in the manufacture of parts resistant to atmosphere, water vapor, water and oxidative acid corrosion.
