Buffer solution is a type of buffer system dedicated to life science research. In biochemical experiments, buffer solution plays an indispensable role. It can resist the influence of a small amount of strong acid and alkali from the outside, and maintain the system closest to the physiological environment. pH value. Both HEPES and PIPES buffers are commonly used buffers in biological experiments.
The pH buffer range of HEPES is 6.8-8.2. It is a zwitterionic biological buffer. It is soluble in water and does not form stable complexes with metal ions. In most cases, it will not interfere with biochemical processes and can be widely used in a variety of Biochemical reactions and used as buffer reagents in certain cell culture media. HEPES is often used in the cell culture medium of various types of organisms; in protein research, PIPES is often used as the component and eluent of the binding buffer in cation exchange chromatography; in DNA research, PIPES is used as calcium phosphate and DNA The buffer for the precipitation formation system, AFM and the buffer for electroporation experiments. In addition, HEPES interferes with the reaction between DNA and restriction enzymes, and is not suitable for Lowry's method to determine protein content. HEPES buffer is often used in the research of organelles and highly variable, pH-sensitive proteins and enzymes, as well as in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. It is a hydrogen ion buffer that can control a constant pH range for a long time. The final concentration is 10-50mmol/L, and the buffering capacity can be achieved when the culture medium contains 20mmol/L HEPES.
The pH buffer range of PIPES is 6.1-7.5, insoluble in water, soluble in NaOH aqueous solution. PIPES is different from buffers containing two (2-hydroxyethyl) amino groups (such as Bis-tris, Bicine), and can not form stable complexes with most metal ions. It is suitable for buffers in solution systems containing metal ions. According to existing research results, PIPES can be used to purify tubulin using phosphocellulose chromatography, to purify recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and to use as a buffer to crystallize transketolase from E. coli. In addition, because PIPES can form free radicals, it is not suitable for redox systems. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because PIPES has a relatively large ionic strength and its pKa value is concentration-dependent.
We must learn to distinguish between the similarities and differences in these reagents, so as to better promote the development and production of our different products. Desheng unanimously adheres to this kind of subtle difference, so that it can continue to develop and produce better products.
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Classification : Hastelloy Alloy Hastelloy Alloy Pipe is a versatile austenitic nickel-chromium-molybdenum-tungsten alloy with enhanced resistance to pitting, crevice corrosion and stress corrosion cracking. The high chromium content provides good resistance to oxidizing media while the molybdenum and tungst en content give good resistance to reducing media.Hastelloy Alloy Pipe is particularly useful for resistance to pitting and crevice corrosion in acid-halide environments.
Classification : Hastelloy Alloy Hastelloy Alloy Pipe is a versatile austenitic nickel-chromium-molybdenum-tungsten alloy with enhanced resistance to pitting, crevice corrosion and stress corrosion cracking. The high chromium content provides good resistance to oxidizing media while the molybdenum and tungst en content give good resistance to reducing media.Hastelloy Alloy Pipe is particularly useful for resistance to pitting and crevice corrosion in acid-halide environments.
Classification : Inconel Alloy Inconel Pipe It has good high-temperature corrosion resistance and oxidation resistance, excellent cold and hot processing and welding process performance, and satisfactory thermal strength and high plasticity below 700. Alloys can be strengthened by cold working, and can also be joined by resistance welding, fusion welding, or brazing.
Galvanized steel pipes are divided into cold galvanized steel pipes and hot galvanized steel pipes, cold galvanized steel pipes have been banned, the latter is also advocated by the state can be used temporarily. In the sixties and seventies, developed countries began to develop new types of pipes, and one after another, galvanized pipes were banned. Nowadays, galvanized pipes are rarely used for cold water in new communities. Hot-dip galvanized pipes are widely used in fire-fighting, electric power, highways and other fields. Product Name Galvanized pipe Technique Longitudinal Electronic Resistance Welding (ERW),High Frequence Welding (HFW) Invoicing by actual weight Outside Diameter 10mm-1020mm or as your request. Surface Galvanized, 3PE, painting, coating oil, steel stamp, etc. Thickness DN50 DN65 DN80 DN100 DN125 DN200 DN450 Trade Terms FOB, CFR, CIF, EXW. Certification BV, ISO, IAF or as per customer. Country Of Origin ShanDong,China Mode Of Payment T/T L/C Delivery Time 7-15 Days Standard GB/T3091 2008/GBT3091-2015/GB/T13793-2016 /GB/T21835-2008 Warehouse Storage Shandong Jintuo Metal Materials Co., Ltd is located in Liaocheng City, the capital of steel tubes. It is a large enterprise specializing in the sales of carbon steel, stainless steel, galvanized color coated products, and copper and aluminum.Our company adopts a flexible business approach and management mechanism to provide good services to customers, while also considering the interests of the owner and the project, in order to ensure the quality of the project. Packing and shipping Our products are exported to multiple overseas countries and regions such as Europe, America, South Asia, Southeast Asia, Africa, and Latin America, and have won the trust and support of customers with excellent quality and service. During the supply process, the company can timely use sufficient funds to adjust the production variety and model of the steel plant, ensuring the use needs during the peak construction period.
Galvanized steel pipes are divided into cold galvanized steel pipes and hot galvanized steel pipes, cold galvanized steel pipes have been banned, the latter is also advocated by the state can be used temporarily. In the sixties and seventies, developed countries began to develop new types of pipes, and one after another, galvanized pipes were banned. Nowadays, galvanized pipes are rarely used for cold water in new communities. Hot-dip galvanized pipes are widely used in fire-fighting, electric power, highways and other fields. Product Name Galvanized pipe Technique Longitudinal Electronic Resistance Welding (ERW),High Frequence Welding (HFW) Invoicing by actual weight Outside Diameter 10mm-1020mm or as your request. Surface Galvanized, 3PE, painting, coating oil, steel stamp, etc. Thickness DN50 DN65 DN80 DN100 DN125 DN200 DN450 Trade Terms FOB, CFR, CIF, EXW. Certification BV, ISO, IAF or as per customer. Country Of Origin ShanDong,China Mode Of Payment T/T L/C Delivery Time 7-15 Days Standard GB/T3091â??â??2008/GBT3091-2015/GB/T13793-2016 /GB/T21835-2008 Warehouse Storage Shandong Jintuo Metal Materials Co., Ltd is located in Liaocheng City, the capital of steel tubes. It is a large enterprise specializing in the sales of carbon steel, stainless steel, galvanized color coated products, and copper and aluminum.Our company adopts a flexible business approach and management mechanism to provide good services to customers, while also considering the interests of the owner and the project, in order to ensure the quality of the project. Packing and shipping Our products are exported to multiple overseas countries and regions such as Europe, America, South Asia, Southeast Asia, Africa, and Latin America, and have won the trust and support of customers with excellent quality and service. During the supply process, the company can timely use sufficient funds to adjust the production variety and model of the steel plant, ensuring the use needs during the peak construction period.
Galvanized steel pipes are divided into cold galvanized steel pipes and hot galvanized steel pipes, cold galvanized steel pipes have been banned, the latter is also advocated by the state can be used temporarily. In the sixties and seventies, developed countries began to develop new types of pipes, and one after another, galvanized pipes were banned. Nowadays, galvanized pipes are rarely used for cold water in new communities. Hot-dip galvanized pipes are widely used in fire-fighting, electric power, highways and other fields. Product Name Galvanized pipe Technique Longitudinal Electronic Resistance Welding (ERW),High Frequence Welding (HFW) Invoicing by actual weight Outside Diameter 10mm-1020mm or as your request. Surface Galvanized, 3PE, painting, coating oil, steel stamp, etc. Thickness DN50 DN6 DN8 DN10 DN125 DN200 DN450 Trade Terms FOB, CFR, CIF, EXW. Certification BV, ISO, IAF or as per customer. Country Of Origin ShanDong,China Mode Of Payment T/T L/C Delivery Time 7-15 Days Standard GB/T3091 2008/GBT3091-2015/GB/T13793-2016 /GB/T21835-2008 Warehouse Storage Shandong Jintuo Metal Materials Co., Ltd is located in Liaocheng City, the capital of steel tubes. It is a large enterprise specializing in the sales of carbon steel, stainless steel, galvanized color coated products, and copper and aluminum.Our company adopts a flexible business approach and management mechanism to provide good services to customers, while also considering the interests of the owner and the project, in order to ensure the quality of the project. Packing and shipping Our products are exported to multiple overseas countries and regions such as Europe, America, South Asia, Southeast Asia, Africa, and Latin America, and have won the trust and support of customers with excellent quality and service. During the supply process, the company can timely use sufficient funds to adjust the production variety and model of the steel plant, ensuring the use needs during the peak construction period.
Galvanized steel pipes are divided into cold galvanized steel pipes and hot galvanized steel pipes, cold galvanized steel pipes have been banned, the latter is also advocated by the state can be used temporarily. In the sixties and seventies, developed countries began to develop new types of pipes, and one after another, galvanized pipes were banned. Nowadays, galvanized pipes are rarely used for cold water in new communities. Hot-dip galvanized pipes are widely used in fire-fighting, electric power, highways and other fields. Product Name Galvanized pipe Technique Longitudinal Electronic Resistance Welding (ERW),High Frequence Welding (HFW) Invoicing by actual weight Outside Diameter 10mm-1020mm or as your request. Surface Galvanized, 3PE, painting, coating oil, steel stamp, etc. Thickness DN50 DN65 DN80 DN100 DN125 DN200 DN450 Trade Terms FOB, CFR, CIF, EXW. Certification BV, ISO, IAF or as per customer. Country Of Origin ShanDong,China Mode Of Payment T/T L/C Delivery Time 7-15 Days Standard GB/T3091 2008/GBT3091-2015/GB/T13793-2016 /GB/T21835-2008 Warehouse Storage Shandong Jintuo Metal Materials Co., Ltd is located in Liaocheng City, the capital of steel tubes. It is a large enterprise specializing in the sales of carbon steel, stainless steel, galvanized color coated products, and copper and aluminum.Our company adopts a flexible business approach and management mechanism to provide good services to customers, while also considering the interests of the owner and the project, in order to ensure the quality of the project. Packing and shipping Our products are exported to multiple overseas countries and regions such as Europe, America, South Asia, Southeast Asia, Africa, and Latin America, and have won the trust and support of customers with excellent quality and service. During the supply process, the company can timely use sufficient funds to adjust the production variety and model of the steel plant, ensuring the use needs during the peak construction period.
Galvanized steel pipes are divided into cold galvanized steel pipes and hot galvanized steel pipes, cold galvanized steel pipes have been banned, the latter is also advocated by the state can be used temporarily. In the sixties and seventies, developed countries began to develop new types of pipes, and one after another, galvanized pipes were banned. Nowadays, galvanized pipes are rarely used for cold water in new communities. Hot-dip galvanized pipes are widely used in fire-fighting, electric power, highways and other fields. Product Name Galvanized pipe Technique Longitudinal Electronic Resistance Welding (ERW),High Frequence Welding (HFW) Invoicing by actual weight Outside Diameter 10mm-1020mm or as your request. Surface Galvanized, 3PE, painting, coating oil, steel stamp, etc. Thickness DN50 DN65 DN80 DN100 DN125 DN200 DN450 Trade Terms FOB, CFR, CIF, EXW. Certification BV, ISO, IAF or as per customer. Country Of Origin ShanDong,China Mode Of Payment T/T L/C Delivery Time 7-15 Days Standard GB/T3091 2008/GBT3091-2015/GB/T13793-2016 /GB/T21835-2008 Warehouse Storage Shandong Jintuo Metal Materials Co., Ltd is located in Liaocheng City, the capital of steel tubes. It is a large enterprise specializing in the sales of carbon steel, stainless steel, galvanized color coated products, and copper and aluminum.Our company adopts a flexible business approach and management mechanism to provide good services to customers, while also considering the interests of the owner and the project, in order to ensure the quality of the project. Packing and shipping Our products are exported to multiple overseas countries and regions such as Europe, America, South Asia, Southeast Asia, Africa, and Latin America, and have won the trust and support of customers with excellent quality and service. During the supply process, the company can timely use sufficient funds to adjust the production variety and model of the steel plant, ensuring the use needs during the peak construction period.
