Luminol Luminescence Principle

Luminol can be oxidized by many substances to produce chemiluminescence, which is the earliest research and most widely used luminescence reagent. The chemiluminescence analysis of metal ions occupies an important position in the entire research and application of chemiluminescence analysis. Some metal ions can enhance the chemiluminescence of the luminol system; some ions can inhibit the chemiluminescence of the luminol system; and some ions can directly oxidize the luminol to produce chemiluminescence. In our drug analysis, we use the catalysis or inhibition of metal ions and compounds for direct or indirect detection. Let's take a look at how luminol "extraordinarily" in drug analysis. Application of Luminol Luminescence System in Pharmaceutical Analysis 1. Direct or indirect detection using the catalysis or inhibition of metal ions and metal ion compounds, such as the determination of sodium nitroprusside (SNP) and catechol compounds. Certain reducing substances such as citric acid, citrate esters, Vc, ascorbic acid, etc. can also be detected by the metal ion-luminol luminescence system. 2. The enhancement or inhibition of the chemiluminescence reaction of luminol by biomolecules and organic substances. DNA, albumin, glucose, and uric acid can enhance the chemiluminescence reaction of luminol, thus establishing their own detection methods. In an alkaline medium, the inhibitory effect of ascorbic acid on the Luminol-K3Fe(CN)6 system can be used to determine ascorbic acid. Luminol-K3Fe(CN)6 series can also be used to monitor glucose and lactic acid. Thiol-containing drugs, promazine, etc. have an inhibitory effect on the luminol luminescence system, and a detection method is proposed accordingly. 3. Preparation of expendable chemiluminescence sensor. Luminol and other reagents are fixed on ion exchange resin to develop a chemiluminescence flow injection sensor, which is used for the determination of Vc. Combine enzyme immobilized column with FIA, use immobilized enzyme reactor, detect by luminol, it has been used for glucose, alcohol, oxalate, lactate, choline, uric acid in serum and urine, etc. Detection. Seeing more of the power of luminol, we suddenly discovered that it is acceptable to us wherever it is. This also fully illustrates its value. If you want to know more about luminol's knowledge and uses, you might as well find its manufacturer-Desheng. I believe you will be worthy of asking.

There are a few points to pay attention to when using ECL luminescent liquid: 1. When using ECL, the previous steps can be carried out in sunlight, but the sensitivity may be slightly reduced when exposed to strong light for too long. Wearing gloves can avoid leaving fingerprints on the film. 2. If the exposure is too long or the protein is too much, the background will deepen and the band strength will lose the linear relationship, while the underexposure will blur the band. 3. The strips on the membrane glow after incubating for about 1 minute in the luminescent working solution. The strong band luminescence is visible to the naked eye in the darkroom, and the low-abundance protein band luminescence is weak or even invisible to the naked eye, but it can be exposed to X-ray film. The light emission time of the strip cannot be judged by visual observation alone. 4. Too high antibody concentration will cause high background or no bands, which will lead to failure! ECL luminescent fluid is mainly made of luminol, isoluminol or isoluminol derivatives, sodium hydroxide and hydrogen peroxide. Desheng produces luminol and isoluminol raw materials, which need to be matched Lye, hydrogen peroxide, and peroxidase can complete the chemiluminescence experiment.

Luminol has become one of the most widely used chemiluminescent reagents due to its advantages of high quantum yield, easy synthesis, and good water solubility. Its development process is the same as the arduous process of human beings. From the beginning, there is not much understanding, to the current glow and fever, it can only be summarized in one sentence: gold always shines. In the future, there may be more uses we don't know to benefit our lives. We only need to learn more about it to discover its unlimited potential as soon as possible. Application in the analysis of inorganic substances Using inorganic ions to enhance or inhibit the chemiluminescence of the luminol system, these metal ions can be measured. It is worth pointing out that some metal ions have higher catalytic activity than metal ions after being combined with complexing agents. For example, using the luminol-H2O2-silver phenanthroline system, the detection limit of silver ions can reach 2.7 10-g/L3. The use of sensitizers can improve the sensitivity of detection. The sensitization effect of triphenylmethane derivatives on the luminol luminescence system. It was found that Coomassie Brilliant Blue G250 had the best sensitization effect on the luminol-H2O2-Cu2+ system. A reverse flow injection technique was used to establish a measurement of Cu2+. The new method has a detection limit of 15. The use of the local concentration effect of surfactants to achieve micelle sensitization has been increasingly used in luminescence analysis. For example, the reversed micellar system FIA method is used to measure V4+, the detection limit is Luminol chemiluminescence analysis has been widely used in various fields, and new methods and new technologies are constantly emerging, especially the combination with FIA, HPLC and other technologies, which overcomes the shortcomings of poor selectivity in luminescence analysis and makes the analysis more flexible. Emblem and accuracy. Because of Rumi's stable performance and low price, practical application research and analysis will have broader application prospects.

Food safety issues have not only seriously affected the health of the people's diet, but also hindered the export trade of my country's agricultural products and food, and undermined the international reputation of my country's food industry. Only by strengthening basic food safety research, especially rapid testing research, can we adapt to the development under the new international situation. How to quickly and effectively do a good job of testing, we have to bring up our powerful chemiluminescence immunoassay to see through which aspects it plays a role in food safety testing. Detection of microorganisms in food The inspection of microorganisms in food is mainly to inspect general pollution and pathogenic bacteria. Escherichia coli O157 H7 is an important food pathogen. The enhanced luminol peroxidase luminescence system can quickly detect E. coli O157:H7. The method is simple and fast. The minimum detection concentration of each bacteria is in the range of 104105CFU/L, and the recovery rate is between 90% and 120%. Detection of biotoxins in food Biotoxin is a general term for a large class of biologically active substances, including animal toxins, plant toxins and microbial toxins. Biotoxins have posed a serious threat to food safety and human health. For example, the chemiluminescence enzyme immunoassay method for detecting aflatoxin M1 in milk uses horseradish peroxidase to catalyze luminol to emit light. Detection of pesticide residues in food Pesticide residues in food can cause chemical food poisoning, and low-concentration residues are also potentially harmful to the human body. At present, the most researched pesticide residue detection technologies are mainly in vivo detection methods, chemical detection methods, enzyme inhibition methods and instrumental analysis methods. modified products After recognizing the major changes brought to us by chemiluminescence reagents, Desheng has independently developed and produced chemiluminescence reagents since 10 years ago, including luminol, isoluminol, and acridinium ester and acridinium ester derivatives. product. These products are not only recognized by many companies in China, but are also well received even if they are exported overseas. Low-key life, high-profile work. What's more, a good product in the eyes of the public always speaks with strength.

Acridine ester (NSP-DMAE-NHS), yellow powder, CAS number: 194357-64-7, is an important chemiluminescence reagent, its chemiluminescence quantum yield is higher than luminol, and it is labeled with acridine ester The conditions are mild, the labeling rate is high, and the separation is not affected after labeling. In addition, the chemiluminescence process of acridinium ester has a rapid reaction and low background, which can emit light in the presence of sodium hydroxide and hydrogen peroxide. During the oxidation reaction, the conjugate is decomposed and does not affect the luminescence of free acridine esters; in addition, acridine esters chemiluminescent reagents have good stability and are easy to store. In addition to its application in immunoassays, acridinium esters can also be used to label oligonucleotide fragment probes for genetic or microbial assays. Acridine ester compounds are very suitable for labeling DNA strands to produce chemiluminescent DNA probes. Modern medical research results show that many diseases such as cancer and genetic diseases are related to DNA mutations, and many infectious diseases are caused by viruses, bacteria or parasites in the environment. The analysis of virus specific sequence DNA is beneficial Control of the epidemic. The detection of DNA sequence and base mutation in its chain is of far-reaching significance in gene screening, early diagnosis and treatment of genetic diseases, and pathogenic gene determination. In nucleic acid hybridization analysis, the preparation of labeled probes with strong specificity and high sensitivity is the key to successful nucleic acid hybridization analysis. Acridinium ester derivatives can be directly labeled on nucleic acid probes without the need for catalysts and the luminescence quantum yield is not affected. In addition, under certain conditions, the labeled acridinium ester on the unhybridized single-stranded DNA is hydrolyzed and destroyed, and only the double-stranded protected acridinium ester formed by hybridization can produce chemiluminescence, and the entire hybridization process can be monitored without separation. Desheng Technology has been developing chemiluminescence reagents for 12 years. After successful development, it has been put into the market and has been widely recognized by customers. The acridinium esters developed and produced have 6 different groups of products. In addition to the acridinium esters, chemiluminescent reagents also include luminol and isoluminol. The luminous efficiency of the acridine ester series is relatively high and belongs to direct luminescence.

[English name] 4-Aminophthalhydrazide [Chinese name]4-Aminophthalhydrazide / 6-Amino-2,3-dihydro-1,4-phthalazinedione [CAS#] 3682-14-2 [Molecular weight] 177.16 [Formula] C8H7N3O2 [Storage condition] Room temperature, away from light and moisture [Appearance] White like powder [Application] Chemiluminescent reagent, commonly used in chemiluminescent immunoassay, such as metal cations and blood detection. [Product advantage] Purity: â?¥ 98% (HPLC), good water solubility, stable process, little difference between batches. Desheng is a chemical reagent company specializing in the research and development of blood test reagents. It has 14 years of research and production experience so far. Luminescent reagent substrate has always been the top priority of the company's research. Although isoluminol is not widely used as luminol, it plays an irreplaceable role in blood test. Enterprises or individuals in need of isoluminol can directly contact 15071057538(Wechat is the same number) . We� look� forward� to� your� calls,� to� join� hands� in� creating� a� better� tomorrow!

[Title] luminol / 3-aminobenzoylhydrazide [English name] 3-amino phthalhydrazide [Chinese name] luminol / 3-aminobenzoylhydrazide / Luminol CAS#521-31-3 [molecular weight] 177.16 [Formula] c8h7n3o2 [storage condition] room temperature, away from light and moisture [chemical properties] soluble in alkaline solution, soluble in dilute acid, almost insoluble in water, insoluble in alcohol. Neutral or light acid solutions show strong bright blue fluorescence when exposed to ultraviolet light. [purpose] chemiluminescent reagent, commonly used in chemiluminescent immunoassay, such as metal cations and blood detection. [luminescent rate detection] the optimal fluorescence wavelength is 425nm (detect the chemiluminescence rate in 60mmk2s2o8100mk2co3, ph11.5 solution). Luminol / luminol is one of the most commonly used liquid-phase chemiluminescent reagents because of its simple structure, easy synthesis, good water solubility and high quantum efficiency. Since Albrecht first reported the chemiluminescent reaction of luminol and oxidant in alkaline solution in 1928, people have been very active in the study of the chemiluminescent system, which makes the chemiluminescence very active Optical systems are used in many fields.

De Sheng is a professional blood testing reagent company. It is a single enterprise with a complete industrial chain in China. Up to now, it has more than 10 years of R&D and production experience. Biobuffer products developed by Liu technical team of the company include Tris, Bicine, Caps, Mops, Taps, EPPS, HEPES, MOPSO, PIPES and so on, which are well received. DeSheng brand Tri (hydroxymethyl) Angelica Tris introduced: Trometamol Name Trimethylol Angelica End Tris CAS# 77-86-1 Molecular Weight]121.135 Molecular Formula Adidine H11NO3 Storage Conditions Room temperature, light-proof and moisture-proof Appearance White Crystal Powder PH Scope] 7-9 Melting Point 168-172 degrees C 1. Dissolve Tris with a small amount of double-steamed water (300-500 ml). After adding HCl, adjust the pH to 7.6 with HCl (1N) or NaOH (1N), and add extra-thick double-steamed water to 1000 ml. This liquid is a reserve liquid, which is stored in refrigerator at 4 C. Uses: Buffer preparation in biochemical and molecular biology experiments; intermediate; preparation of surfactants and vulcanization accelerators; preparation of water-soluble polymers containing Tris structural units as paint dispersants; preparation of indoor formaldehyde adsorption materials, etc., Tris is also used as titration standard. 1. Buffering properties] Tris is a weak base, and its pKa is 8.1 at room temperature (25 C); according to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2. Desheng products include good's buffer: Bicine, Caps, EPPS, MOPS, TAPS, HEPES, MOPSO, PIPES; blood vessel extracting additives: serum separating gel, sodium glycerin, glycerin, EDTA dipotassium, EDTA tripotassium, coagulant, planning agent, sodium citrate, planning potassium; chromogenic substrates: TOOS, TOPS, ADOS, ADP S, ALPS, DAOS, HDAOS, MADB, MAOS, PEP, etc. Luminescent reagents: Azidine ester, Luminol, Azidine salt and isoluminol. All in vitro diagnostic reagents are purified by HPLC analysis method and the purity is more than 99%. As the manufacturer develops and sells, the DeSheng color substrates are in stock and the quality is guaranteed. Enterprise friends who need color raw materials can contact Desheng, address C8, Guanggu United Science and Technology City, Ezhou, Hubei Province, and look forward to your visit!

Measurement: Luminous Intensity Data, Photometric Data, Luminous Intensity Distribution, Zonal Luminous Flux, Luminaries Efficiency, Luminance Distribution(Option), Coefficient Of Utilization, Luminance Limitation Curves Glare, Maximum Ratio of Distance to Height, Equal Illuminance Diagrams, Curves of Luminaires VS Lighting Area, Isocandela Diagrams, Efficient Luminescence Angle, EEI, UGR, etc. Features: he near field detector moves together with the big mirror in a line. The big mirror and the far field detector move synchronously.The burning position of the lumainares will be kept without moving at all, and the detector will always sense the light directly from the luminares. The rotary motor is from Japan MITSUBISHI MOTORS and the angle decode system is from Germany. They help the goniophotometer rotating smoothly with high accuracy. It is very stable when start and stop. The working principles are according to IESNA and CIE. The LSG-6000 completely meet the LM-80, LM-79, LM-75, GB, EN and CIE121-1996 standards. Special collimation device with cross laser line help you installing the position of the luminaires under test conveniently and accurately. Specifications: The mirror and detector rotates around the luminaire under test with an angle of () vertical axis ±180 (or 0-360) and the luminaire rotates around itself with an angle of (C) horizontal axis ±180 (or 0-360 ). The accuracy of angle: 0.05, Resolution of angle: 0.001 Accuracy of Goniophotometry detector: Constant temperature photo detector DIN5032-6/CIE pub1. No. 69 Class L LISUN goniophotometer software can export CIE, IES, LDT and other format files. These kinds of format files can be used via other illumination and luminaire design software such as DiaLux. Connect the goniophotometer to PC via USB cable. English version software can run on Win7, Win8 or Win10, Win11 LSG-6000 can be used together with a CCD Spectroradiometer to test spatial CCT Distribution and other Spectral color parameters. We call this system Goniospectroradiometer (LSG-6000CCD)â??. LSG-6000CCD Goniospectroradiometer = LSG-6000 Goniophotometer System + LPCE-2 Integrating sphere Spectroradiometer System. Please click to download the LSG-6000CCD Brochure The LSG-6000CCD software and hardware support the plant lighting PAR, PPF and PPFD spatial distribution test , and it can export IES/LDT files