1. Basic nature
The application of acridine compounds in chemiluminescence detection has the following advantages:
Low background luminescence and high signal-to-noise ratio;
Less interference factors in luminescence reaction;
The light release is fast and concentrated, the luminous efficiency is high, and the luminous intensity is large;
It is easy to link with protein and the photon yield does not decrease after linking;
The markers are stable (storable for several months at 2-8C), they have good solubility in water, are stable and are not easily hydrolyzed.
The chemiluminescence of acridine compounds does not require a catalyst and can emit light in a dilute alkaline solution containing H2O2. The luminescence of the compound is flash type. After adding the luminescence starter, the emitted light intensity reaches the maximum in about 0.5s, and the half-life is about 1s.
Acridine compounds are used in chemiluminescence immunoassay (CLIA). That is, the antibody or antigen is labeled with acridine salt, and HNO3+H2O2 and NaOH are used as the luminescence priming reagent. Some add Triton X-100, CTAC, Tween-20 and other surfactants to the luminescence priming reagent to enhance luminescence.
Acridine compounds are commonly used in environmental analysis, immunoassay, enzyme activity analysis and labeling oligonucleotide fragments, etc., and are used clinically to determine bacteria, viruses or other disease markers.
2. Acridine ester DMAE-NHS or NSP-DMAE-NHS
Acridine ester DMAE-NHS or NSP-DMAE-NHS is an ideal chemiluminescence immunoassay labeling substance with good luminescence performance. Compared with the widely used acridinium ester AE-NHS, the luminescence intensity of the acridinium ester DMAE-NHS or NSP-DMAE-NHS and antibody (Ab) conjugates is higher, and the luminescence dynamics of the two are very similar. Both are flash-type luminescence and both reach the highest at 0.4s, but the thermal stability of DMAE-NHS or NSP-DMAE-NHS is significantly higher than that of AE-NHS. AE-NHS is only stable in acidic solutions, when pH>6.3 It is easy to hydrolyze later, but DMAE-NHS or NSP-DMAE-NHS is not. For example, at room temperature, it is very stable in a PB buffer with a pH of 7.0. After 16 days, the luminescence activity is only reduced by 3.6%. Their luminescence thermal stability with protein conjugates is similar to this.
The acridinium ester DMAE-NHS or NSP-DMAE-NHS is easy to label biomolecules and the luminescent activity and biological activity of the labeled compound are stable. The synthesized luminescent label is applied to chemiluminescence immunoassay with high sensitivity and other ideals. For example, the minimum detection limit of TSH is 0.007 mIu/L. Therefore, the acridinium ester DMAE-NHS or NSP-DMAE-NHS has good application value for the research of chemiluminescence analysis and chemiluminescence immunoassay.
3. Acridine Sulfonamide NSP-SA-NHS
Acridine sulfonamide is a very effective
Desheng is a new scientific and technological enterprise specializing in the research and development, production and sales of blood collection additives, chemiluminescence reagents, biological buffers and in vitro diagnostic reagents. Acridine ester series of products are the company's research and development of ultra-high sensitivity chemiluminescence substrate reagents. Under alkaline conditions, the acridine ester molecules can be attacked by hydrogen peroxide to form dioxane, which is unstable and decomposes into CO2 and n-methylacridone in the electron excited state. When it returns to the ground state, it emits light with wavelength of 430 nm. It does not need catalyst, and the luminescence system is simple. Desheng has seven acridine ester products with different groups: 1. Ae-nhs (traditional acridine ester); 2. Dmae-nhs; 3. Me-dmae-nhs; 4. Nsp-dmae-nhs; 5. Nsp-sa-nhs; 6. Nsp-sa; 7. Nsp-sa-adh. These acridine esters produced by Desheng have the characteristics of high purity and small difference between batches. Besides, it provides other immune reagents for matching use and provides a complete set of technical services and technical support.
Application of nitrocellulose Nitrocellulose, also known as cellulose nitrate or nitrocellulose, is an organic polymer compound with the chemical formula (C6H7N3O11) n. It is the product of the esterification reaction between cellulose and nitrate, usually in the form of white or slightly yellow cotton fluff, insoluble in water, but soluble in organic solvents such as esters and acetone Nitrocellulose, also known as cellulose nitrate ester, abbreviated as NC, commonly known as nitrocellulose, is the product of the reaction between cellulose and nitrate. Nitrocellulose made from cotton fibers is called nitrocellulose. Nitrocellulose is a white fibrous polymer that is resistant to water, dilute acids, weak alkalis, and various oils. Different degrees of polymerization result in different strengths, but they are all thermoplastic substances. Easy to change color under sunlight and highly flammable. Attention should be paid to safety in production, processing, packaging, storage, transportation, sales, and use.
Acridine Ester Luminescence Analysis Study on DNA Damage of Formaldehyde and Acetaldehyde Acridine ester can be used as a chemiluminescent indicator with a good intercalation structure of DNA double helix. Because low concentration of formaldehyde causes DNA breakage and damage, high concentration of formaldehyde causes DNA strand cross-linking, and acetaldehyde only causes DNA strand breakage damage. The chemiluminescence detection method for DNA break damage has the advantages of simplicity and sensitivity, and provides a simple research method for the study of environmental pollutant damage to DNA. Acridinium-labeled streptavidin Acridine ester DMAE -NHS was used to label streptavidin, one strain of TSH monoclonal antibody was labeled with biotin, and the other strain of TSH monoclonal antibody was coated on 96 micro-well plates, and the TSHBAS-CLIA method was established. Acridinium ester-labeled antibody or antigen, biotin-labeled antibody or antigen, streptavidin-coated magnetic beads with carboxyl or Tosyl (p-tosyl) functional groups; the particle size of the magnetic beads is 0.1-5um . Since biotin and streptavidin are combined in a ratio of 4:1, the acridinium ester-biotin-antigen-antibody complex is also combined with streptavidin in a 4-fold ratio, so that the luminescence signal is also enlarged. 4 times, the detection sensitivity is higher. Acridine ester-labeled antibody for rapid determination of antibody level in tuberculosis patients Using acridinium ester-labeled goat anti-human IgG antibodies, a method for the determination of IgG in human serum has been established. The labeling method has mild conditions, is simple and easy to implement, and its standard curve ranges from 7.5 to 125 ng IgG/ml. The results of the determination of antibody levels in the serum of tuberculosis patients are good. Acridine ester-labeled nucleic acid probe method can quickly detect Escherichia coli Use the nucleic acid probe method to quickly detect E. coli O157:H7. The method is to detect E. coli O157:H7 by using a specific DNA probe labeled with acridine ester. The nucleic acid probe method detects E. coli O157:H7 with high specificity and sensitivity. The minimum concentration of E. coli O157:H7 is about 106cfu/ml. The result of detection of E. coli O157:H7 is consistent with the national standard method; for O157 : H7 identification time is only 30 minutes, which is simple and quick. The conclusion is that the nucleic acid probe method can be used for the rapid detection of E. coli O157:H7.
[Title] acridine ester me-dmae-nhs [English name] 2 ', 6' - demethylcarbonylphenyl 10-methyl-9-acridinecarboxylate 4 '- NHS ester triflate [molecular weight] 632.56 [Formula] c29h23f3n2o9s [appearance] yellow solid or powder [storage conditions] refrigerate at 2-8 , seal, dry and keep away from light [purpose] it can be used to mark proteins, antigens, antibodies, nucleic acids (DNA, RNA), etc. [product advantage] Purity: 98% (HPLC), good water solubility, stable process, small batch difference.
Fomates are the simplest carboxylate anion or esters of formic acid. These formate chemical are available in different grades and compositions based upon the application requirements. Every batch of the formate chemical including ammonium formate are tested on various parameters and packed with precision. We can also offers custom formulations of these ammonium formates and ammonium crystals based upon the demands of the clients. Ammonium Formate Ammonium Formate Leading manufacturer and exporter of Ammonium Formate. We are producing Ammonium Formate (EP/LR Grade) and Ammonium Formate (AR/GR Grade).
[Title]acridine salt nsp-sa [English name] (tosyl) carbamoyl) acridinium-10-yl) propane-1-sulfonate (nsp-sa) [CAS#]211106-69-3 [molecular weight] 584.66 [Formula] c28h28n2o8s2 [appearance] yellow solid or powder [storage conditions] refrigerate at 2-8 , seal, dry and keep away from light [purpose] it can be used to mark proteins, antigens, antibodies, nucleic acids (DNA, RNA), etc. The dilute solution of acridine and its salts shows purple or green fluorescence, while the dilute solution of salts has green fluorescence. After dilution, it becomes free acridine due to saltwater hydrolysis, showing purple fluorescence. Acridine salt is widely used as photocatalyst and dye preparation. [product advantage] Purity: 98% (HPLC), good water solubility, stable process, small batch difference.
[Title] acridine salt nsp-sa-nhs [English name] 3-[9-(((3-(N-succinimidyloxycarboxypropyl)[4-methxylphenyl]sulfonyl)amine)carboxyl]-10-acridiniumyl)-1-propanesulfonatei CAS#199293-83-9 [molecular weight] 681.73 [Formula] c32h31n3o10s2 [appearance] yellow solid or powder [storage conditions] refrigerate at 2-8 , seal, dry and keep away from light [purpose] it can be used to mark proteins, antigens, antibodies, nucleic acids (DNA, RNA), etc. [product advantage] Purity: 98% (HPLC), good water solubility, stable process, small batch difference. The related compounds have been proved to be very advantageous chemiluminescence markers, with stability, activity and sensitivity higher than those of radioisotopes.
A phosphate is a chemical derivative of phosphoric acid. The phosphate ion is an inorganic chemical, the conjugate base that can form many different salts. In organic chemistry, a phosphate, or organophosphate, is an ester of phosphoric acid Origins : Syria, egypt, jordan
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