Chemiluminescence Analysis Of Luminol

Luminol has become one of the most widely used chemiluminescent reagents due to its advantages of high quantum yield, easy synthesis, and good water solubility. Its development process is the same as the arduous process of human beings. From the beginning, there is not much understanding, to the current glow and fever, it can only be summarized in one sentence: gold always shines. In the future, there may be more uses we don't know to benefit our lives. We only need to learn more about it to discover its unlimited potential as soon as possible. Application in the analysis of inorganic substances Using inorganic ions to enhance or inhibit the chemiluminescence of the luminol system, these metal ions can be measured. It is worth pointing out that some metal ions have higher catalytic activity than metal ions after being combined with complexing agents. For example, using the luminol-H2O2-silver phenanthroline system, the detection limit of silver ions can reach 2.7 10-g/L3. The use of sensitizers can improve the sensitivity of detection. The sensitization effect of triphenylmethane derivatives on the luminol luminescence system. It was found that Coomassie Brilliant Blue G250 had the best sensitization effect on the luminol-H2O2-Cu2+ system. A reverse flow injection technique was used to establish a measurement of Cu2+. The new method has a detection limit of 15. The use of the local concentration effect of surfactants to achieve micelle sensitization has been increasingly used in luminescence analysis. For example, the reversed micellar system FIA method is used to measure V4+, the detection limit is Luminol chemiluminescence analysis has been widely used in various fields, and new methods and new technologies are constantly emerging, especially the combination with FIA, HPLC and other technologies, which overcomes the shortcomings of poor selectivity in luminescence analysis and makes the analysis more flexible. Emblem and accuracy. Because of Rumi's stable performance and low price, practical application research and analysis will have broader application prospects.

Luminol can be oxidized by many substances to produce chemiluminescence, which is the earliest research and most widely used luminescence reagent. The chemiluminescence analysis of metal ions occupies an important position in the entire research and application of chemiluminescence analysis. Some metal ions can enhance the chemiluminescence of the luminol system; some ions can inhibit the chemiluminescence of the luminol system; and some ions can directly oxidize the luminol to produce chemiluminescence. In our drug analysis, we use the catalysis or inhibition of metal ions and compounds for direct or indirect detection. Let's take a look at how luminol "extraordinarily" in drug analysis. Application of Luminol Luminescence System in Pharmaceutical Analysis 1. Direct or indirect detection using the catalysis or inhibition of metal ions and metal ion compounds, such as the determination of sodium nitroprusside (SNP) and catechol compounds. Certain reducing substances such as citric acid, citrate esters, Vc, ascorbic acid, etc. can also be detected by the metal ion-luminol luminescence system. 2. The enhancement or inhibition of the chemiluminescence reaction of luminol by biomolecules and organic substances. DNA, albumin, glucose, and uric acid can enhance the chemiluminescence reaction of luminol, thus establishing their own detection methods. In an alkaline medium, the inhibitory effect of ascorbic acid on the Luminol-K3Fe(CN)6 system can be used to determine ascorbic acid. Luminol-K3Fe(CN)6 series can also be used to monitor glucose and lactic acid. Thiol-containing drugs, promazine, etc. have an inhibitory effect on the luminol luminescence system, and a detection method is proposed accordingly. 3. Preparation of expendable chemiluminescence sensor. Luminol and other reagents are fixed on ion exchange resin to develop a chemiluminescence flow injection sensor, which is used for the determination of Vc. Combine enzyme immobilized column with FIA, use immobilized enzyme reactor, detect by luminol, it has been used for glucose, alcohol, oxalate, lactate, choline, uric acid in serum and urine, etc. Detection. Seeing more of the power of luminol, we suddenly discovered that it is acceptable to us wherever it is. This also fully illustrates its value. If you want to know more about luminol's knowledge and uses, you might as well find its manufacturer-Desheng. I believe you will be worthy of asking.

Luminol, is a yellow crystal or beige powder at room temperature and is a relatively stable chemical reagent. At the same time, luminol is a strong acid, which has a certain irritating effect on eyes, skin and respiratory tract. It is one of the oldest and most commonly used reagents. It can be oxidized by peroxide under alkaline conditions and emit light at the same time. The redox reaction between luminol and peroxide requires a catalyst, which is generally more Valence metal ions, peroxidases such as iron, horseradish peroxidase, etc. This method is often used to detect the content of peroxides, heavy metals, peroxidase, and the derived free radicals, for toxicant analysis And analysis methods based on peroxidase and glucose oxidase. Under normal circumstances, the chemiluminescence reaction between luminol and hydrogen peroxide is very rapid in the presence of certain catalysts. The most commonly used catalyst is metal ions. In a large concentration range, the concentration of metal ions is directly proportional to the luminescence intensity, so that chemiluminescence analysis of certain metal ions can be performed. This reaction can be used to analyze organic compounds containing metal ions to achieve Very high sensitivity. The second is to use the inhibitory effect of organic compounds on the chemiluminescence reaction of luminol to determine the organic compounds that have a quenching effect on the chemiluminescence reaction. The third is the indirect determination of inorganic or organic compounds through coupling reactions. Luminol Luminescence Principle One is that sodium hypochlorite oxidizes luminol to make it glow; The second is that hydrogen peroxide reacts with sodium hypochlorite to generate oxygen and oxidize luminol to make it glow: The first is the equation for the reaction of sodium hypochlorite and hydrogen peroxide: NaClO+ H2O2== NaCl + O2+ H2O Secondly, when luminol reacts with hydroxide, a double negative ion (Dianion) is formed, which can be oxidized by oxygen generated by the decomposition of hydrogen peroxide, and the product is an organic peroxide. The peroxide is very unstable and immediately decomposes nitrogen (Luminol is not oxidized by organic oxidants such as dimethyl sulfoxide to generate nitrogen, but to generate nitrogen-containing organics) to generate excited 3-aminophthalic acid . In the transition from the excited state to the ground state, the released energy exists in the form of photons with a wavelength in the blue part of visible light. The method of indirect determination by coupling refers to the combination of one reaction that can produce or consume chemiluminescence reactants with another chemiluminescence reaction, so that indirect chemiluminescence determination of certain substances can be achieved. This method is used when determining the purity of certain substrate enzymes.

Acridine ester (NSP-DMAE-NHS), yellow powder, CAS number: 194357-64-7, is an important chemiluminescence reagent, its chemiluminescence quantum yield is higher than luminol, and it is labeled with acridine ester The conditions are mild, the labeling rate is high, and the separation is not affected after labeling. In addition, the chemiluminescence process of acridinium ester has a rapid reaction and low background, which can emit light in the presence of sodium hydroxide and hydrogen peroxide. During the oxidation reaction, the conjugate is decomposed and does not affect the luminescence of free acridine esters; in addition, acridine esters chemiluminescent reagents have good stability and are easy to store. In addition to its application in immunoassays, acridinium esters can also be used to label oligonucleotide fragment probes for genetic or microbial assays. Acridine ester compounds are very suitable for labeling DNA strands to produce chemiluminescent DNA probes. Modern medical research results show that many diseases such as cancer and genetic diseases are related to DNA mutations, and many infectious diseases are caused by viruses, bacteria or parasites in the environment. The analysis of virus specific sequence DNA is beneficial Control of the epidemic. The detection of DNA sequence and base mutation in its chain is of far-reaching significance in gene screening, early diagnosis and treatment of genetic diseases, and pathogenic gene determination. In nucleic acid hybridization analysis, the preparation of labeled probes with strong specificity and high sensitivity is the key to successful nucleic acid hybridization analysis. Acridinium ester derivatives can be directly labeled on nucleic acid probes without the need for catalysts and the luminescence quantum yield is not affected. In addition, under certain conditions, the labeled acridinium ester on the unhybridized single-stranded DNA is hydrolyzed and destroyed, and only the double-stranded protected acridinium ester formed by hybridization can produce chemiluminescence, and the entire hybridization process can be monitored without separation. Desheng Technology has been developing chemiluminescence reagents for 12 years. After successful development, it has been put into the market and has been widely recognized by customers. The acridinium esters developed and produced have 6 different groups of products. In addition to the acridinium esters, chemiluminescent reagents also include luminol and isoluminol. The luminous efficiency of the acridine ester series is relatively high and belongs to direct luminescence.

Food safety issues have not only seriously affected the health of the people's diet, but also hindered the export trade of my country's agricultural products and food, and undermined the international reputation of my country's food industry. Only by strengthening basic food safety research, especially rapid testing research, can we adapt to the development under the new international situation. How to quickly and effectively do a good job of testing, we have to bring up our powerful chemiluminescence immunoassay to see through which aspects it plays a role in food safety testing. Detection of microorganisms in food The inspection of microorganisms in food is mainly to inspect general pollution and pathogenic bacteria. Escherichia coli O157 H7 is an important food pathogen. The enhanced luminol peroxidase luminescence system can quickly detect E. coli O157:H7. The method is simple and fast. The minimum detection concentration of each bacteria is in the range of 104105CFU/L, and the recovery rate is between 90% and 120%. Detection of biotoxins in food Biotoxin is a general term for a large class of biologically active substances, including animal toxins, plant toxins and microbial toxins. Biotoxins have posed a serious threat to food safety and human health. For example, the chemiluminescence enzyme immunoassay method for detecting aflatoxin M1 in milk uses horseradish peroxidase to catalyze luminol to emit light. Detection of pesticide residues in food Pesticide residues in food can cause chemical food poisoning, and low-concentration residues are also potentially harmful to the human body. At present, the most researched pesticide residue detection technologies are mainly in vivo detection methods, chemical detection methods, enzyme inhibition methods and instrumental analysis methods. modified products After recognizing the major changes brought to us by chemiluminescence reagents, Desheng has independently developed and produced chemiluminescence reagents since 10 years ago, including luminol, isoluminol, and acridinium ester and acridinium ester derivatives. product. These products are not only recognized by many companies in China, but are also well received even if they are exported overseas. Low-key life, high-profile work. What's more, a good product in the eyes of the public always speaks with strength.

[Title] luminol / 3-aminobenzoylhydrazide [English name] 3-amino phthalhydrazide [Chinese name] luminol / 3-aminobenzoylhydrazide / Luminol CAS#521-31-3 [molecular weight] 177.16 [Formula] c8h7n3o2 [storage condition] room temperature, away from light and moisture [chemical properties] soluble in alkaline solution, soluble in dilute acid, almost insoluble in water, insoluble in alcohol. Neutral or light acid solutions show strong bright blue fluorescence when exposed to ultraviolet light. [purpose] chemiluminescent reagent, commonly used in chemiluminescent immunoassay, such as metal cations and blood detection. [luminescent rate detection] the optimal fluorescence wavelength is 425nm (detect the chemiluminescence rate in 60mmk2s2o8100mk2co3, ph11.5 solution). Luminol / luminol is one of the most commonly used liquid-phase chemiluminescent reagents because of its simple structure, easy synthesis, good water solubility and high quantum efficiency. Since Albrecht first reported the chemiluminescent reaction of luminol and oxidant in alkaline solution in 1928, people have been very active in the study of the chemiluminescent system, which makes the chemiluminescence very active Optical systems are used in many fields.

[English name] 4-Aminophthalhydrazide [Chinese name]4-Aminophthalhydrazide / 6-Amino-2,3-dihydro-1,4-phthalazinedione [CAS#] 3682-14-2 [Molecular weight] 177.16 [Formula] C8H7N3O2 [Storage condition] Room temperature, away from light and moisture [Appearance] White like powder [Application] Chemiluminescent reagent, commonly used in chemiluminescent immunoassay, such as metal cations and blood detection. [Product advantage] Purity: â?¥ 98% (HPLC), good water solubility, stable process, little difference between batches. Desheng is a chemical reagent company specializing in the research and development of blood test reagents. It has 14 years of research and production experience so far. Luminescent reagent substrate has always been the top priority of the company's research. Although isoluminol is not widely used as luminol, it plays an irreplaceable role in blood test. Enterprises or individuals in need of isoluminol can directly contact 15071057538(Wechat is the same number) . We� look� forward� to� your� calls,� to� join� hands� in� creating� a� better� tomorrow!

1. Basic nature The application of acridine compounds in chemiluminescence detection has the following advantages: Low background luminescence and high signal-to-noise ratio; Less interference factors in luminescence reaction; The light release is fast and concentrated, the luminous efficiency is high, and the luminous intensity is large; It is easy to link with protein and the photon yield does not decrease after linking; The markers are stable (storable for several months at 2-8C), they have good solubility in water, are stable and are not easily hydrolyzed. The chemiluminescence of acridine compounds does not require a catalyst and can emit light in a dilute alkaline solution containing H2O2. The luminescence of the compound is flash type. After adding the luminescence starter, the emitted light intensity reaches the maximum in about 0.5s, and the half-life is about 1s. Acridine compounds are used in chemiluminescence immunoassay (CLIA). That is, the antibody or antigen is labeled with acridine salt, and HNO3+H2O2 and NaOH are used as the luminescence priming reagent. Some add Triton X-100, CTAC, Tween-20 and other surfactants to the luminescence priming reagent to enhance luminescence. Acridine compounds are commonly used in environmental analysis, immunoassay, enzyme activity analysis and labeling oligonucleotide fragments, etc., and are used clinically to determine bacteria, viruses or other disease markers. 2. Acridine ester DMAE-NHS or NSP-DMAE-NHS Acridine ester DMAE-NHS or NSP-DMAE-NHS is an ideal chemiluminescence immunoassay labeling substance with good luminescence performance. Compared with the widely used acridinium ester AE-NHS, the luminescence intensity of the acridinium ester DMAE-NHS or NSP-DMAE-NHS and antibody (Ab) conjugates is higher, and the luminescence dynamics of the two are very similar. Both are flash-type luminescence and both reach the highest at 0.4s, but the thermal stability of DMAE-NHS or NSP-DMAE-NHS is significantly higher than that of AE-NHS. AE-NHS is only stable in acidic solutions, when pH>6.3 It is easy to hydrolyze later, but DMAE-NHS or NSP-DMAE-NHS is not. For example, at room temperature, it is very stable in a PB buffer with a pH of 7.0. After 16 days, the luminescence activity is only reduced by 3.6%. Their luminescence thermal stability with protein conjugates is similar to this. The acridinium ester DMAE-NHS or NSP-DMAE-NHS is easy to label biomolecules and the luminescent activity and biological activity of the labeled compound are stable. The synthesized luminescent label is applied to chemiluminescence immunoassay with high sensitivity and other ideals. For example, the minimum detection limit of TSH is 0.007 mIu/L. Therefore, the acridinium ester DMAE-NHS or NSP-DMAE-NHS has good application value for the research of chemiluminescence analysis and chemiluminescence immunoassay. 3. Acridine Sulfonamide NSP-SA-NHS Acridine sulfonamide is a very effective

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