What is PIPES buffer
PIPES Buffer (CAS5625-37-6) chemical name is 1,4-piperazine diethanesulfonic acid, which is a zwitterionic biological buffer. It is a white powder solid at room temperature. PIPES Buffer is not easily soluble in water (1g/L (100C). It can be dissolved by mixing equimolar sodium salt solution and titrating to the appropriate pH value. For example, PIPES Buffer (CAS76836-02-7) and PIPES sesquisodium salt ( CAS100037-69-2).
Application of PIPES Buffer
PIPES Buffer is widely used in cell culture, chromatography, cosmetics, diagnostic testing, protein purification and electron microscopy research. PIPES Buffer is similar to MES Buffer and MOPS Buffer (also produced by Desheng). This Good's Buffer lacks the ability to form complexes with most metal ions, so it is recommended to be used as a non-coordinating buffer in metal ion solutions.
Advantages of PIPES Buffer
According to related literature, the use of NaOH-PIPES buffered glutaraldehyde solution in the ultrastructure of various tissues, such as human skin, human hypertrophic scars, mollusk neuron tissue, etc., the results show that more organelles and organelles can be observed Between the details. The cytoplasm is more uniform in consistency. Compared with NaOH-PIPES buffer, microfilaments and microtubules are more common in PIPES tissues. In addition, PIPES Buffer is proven to be comparable in living cells.
What is TRICINE buffer
TRICINE Buffer (CAS 5704-04-1), a zwitterionic biological buffer containing amine and acid groups. This product is a white crystalline powder, slightly soluble in water (the solubility is 1.8g/100ml water).
Application of TRICINE Buffer
TRICINE is widely used in cell culture and electrophoresis research. As a buffer for cell culture research, TRICINE can inhibit the unexpected growth of mycoplasma in animal tissue culture. It has also been used in bacterial culture to prevent precipitation of iron salts. The Good's buffer can form complexes with most metal ions, such as Mg, Ca, Co, Cu, Ni, and Zn, so the stability constant and concentration should be considered when choosing the buffer. As a buffer for electrophoresis research, Tricine has been used as an alternative to protocols using the controlled substance barbital.
Advantages of TRICINE Buffer
TRICINE has a higher negative (more negative) charge than glycine, making it migrate faster. In addition, its high ionic strength results in more ion movement and less protein movement, so that low-molecular-weight proteins can be separated in a lower percentage of acrylamide gels. Tricine has been proven to be used to separate proteins in the range of 1 to 100 kDa by electrophoresis. In ten tests using firefly luciferase for ATP determination, 25mmol/L tricine buffer was found to be the most effective buffer.
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Introduction: 4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells. Application: HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile. Buffer solution preparation: (1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L. (2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4�ºC or -20�ºC. Purpose: 1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA 2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase 3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer
Applications: Food ingredients for bakery, beverage, confectionery industry It is used as a raising agent in biscuits, cookies, bread, cakes, farsan, jaggery & confectionery. It acts like a buffer and carbonation agent in beverages, electrolyte and rehydration solutions.
Applications: In Animal feed stuff Nutrition feed for Poultry, Layers, Cattle. Primarily used as a dairy cow feed supplement, the buffering capability of Natural Soda's pure and natural feed grade sodium bicarbonate helps stabilize rumen pH by reducing acidic conditions. Improves calcium absorption and eggshell quality of laying hens during peak production.
Formula:KAl(SO4)2 12H2O Relative Molecular Weight:474.39 CAS NO,: 7784-24-9 EINECS No.:5637-689-7 HS CODE.: 2833301000 Appearance: lump, white crystal, white powder Properties:Colorless, transparent, particle or crystalline powder, odorless, nontoxic, tastes slightly sweet and puckery, astringent, easily soluble in water, its water solution appears acid,is would lose crystal water and become white powder on heating Usage; Food industry: Buffering agents and leavening agents in food-grade products. Chemical Industry: Water Flocculant, aluminum tanning agents and after-treating agents for leather tanning in the leather industry, dyeing agents,Astringents,mordants etc. Items Specifications Potassium aluminum sulfate [AlK(SO4)2.12H2O] % 99.5 Plumbum(Pb) mg/kg 5.0 Arsenic (As) mg/kg 2.0 Fluorine (F) mg/kg 30.0 Selenium (Se) mg/kg 30.0 Moisture % 4.0
Chinese Name: piperazine-N, N '- bis (2-ethanesulfonic acid) sesodium salt English Name: PIPES sesquisodium salt CAS No.: 100037-69-2 Molecular formula: C16H33N4Na3O12S4 Molecular weight: 670.68 Storage conditions: Store at RT Application: biological buffer
1. Chemical Name: Sodium Pyrophosphate 2. Formula: Na4P2O7, Na4P2O7, 10H2O 3. Molecular weight: 265.90, 446.06 4. Specificity: Colorless or white crystals, crystalline or granular powder. It is easily soluble in water, but is insoluble in alcohol. Density: 2.45, relative density: 2.45, melting point: 890? . The decahydrate effloresces slightly in dry air. pH of 1% solution is about 10. 5. Quality Standard: 6. Usage: It is mainly used as detergent, electroplating . ctFor food grade it is mainly used as emulsifier, buffer, nutrient, sequestrant, texturizer 7. Packing: 25kgs net paper or plastic woven bags. 8. Storage and Transport: It should be stored in a dry, ventilative and clean warehouse kept away from moisture and hot, Furthermore, it should be stored separately from poisonous Substances.
Main Information: Sodium Sesquicarbonate Molecular Formula: Na2CO3.NaHCO3.2H2O CAS Number: 533-96-0 EC Number: 205-580-9 Manufacturer: TATA Chemicals Europe Appearance: Granular Also Known As: Trisodium Hydrogendicarbonate Sesqui CREX Key Properties: Sodium Sesquicarbonate (Borax Substitute) is a unique alkaline product that is ideal for a wide range of household, personal care and industrial uses. Manufactured by Tata Chemicals Europe at its dedicated plant in the UK, Sodium Sesquicarbonate (Borax Substitute)is a free-flwing, non-irritant white powder. It is produced by the recrystallisation of Sodium Carbonate and Sodium Bicarbonate, to form a single crystal of Sodium Sesquicarbonate (Borax Substitute)Na2CO3.NaHCO3.2H2O. Sodium Sesquicarbonate (Borax Substitute)avoids pH surges seen with blends of Sodium Bicarbonate and Sodium Carbonate due to their different dissolution rates. The varied and valuable properties of Sodium Sesquicarbonate (Borax Substitute), such as water softening, high solubility, mild alkalinity, free flow and excellent buffering capacity, lead to uses in a wide range of applications.
Dipotassium Phosphate, E340, CAS no.7758-11-4, white powder, water-soluble salt used as a fertilizer, food additive and buffering agent. The manufacturing process of Dipotassium Phosphate is via chemcial synthesis, Phosphoric acid and Potassium hydroxide as raw materials. Dipotassium Phosphate is a common source of phosphorus and potassium. It functions as a stabilizing salt, buffer, and sequestrant. It is used as a food additive or preservative is highly effective in preventing coagulation, and is commonly used in non-dairy creamer as a buffering agent. As an experienced Dipotassium Phosphate manufacturer and supplier, we has been supplying and exporting Dipotassium Phosphate for almost 10 years, please be assured to buy from us. Any inquiries about price and the market trend please feel free to contact us, we will reply you within 1 working day. Dipotassium Phosphate Specification Item Unit Dipotassium phosphate 98% Appearance White powder K2HPO4ï¼?as dry basisï¼? %(w/w) min 98.0 Water-insoluble %(w/w) max 0.2 Arsenic (As) mg/kg max 3 Fluoride (as F) mg/kg max 10 Loss on Drying %(w/w) max 2.0 Pb mg/kg max 2
