The new crown virus has broken the otherwise peaceful life and messed up people's lives. It is because of the extremely high risk of infection that everyone is afraid and cautious. When difficulties arise, methods to solve them will follow. In order to determine whether they are infected with the new crown virus, nucleic acid testing has made its debut. Nucleic acid testing is an important means to confirm the new coronavirus, and it is also one of the important processes for patients to confirm the diagnosis. To do nucleic acid testing,
Nucleic acid detection has the characteristics of early diagnosis, high sensitivity and specificity, and is the "gold standard" for diagnosing new coronary pneumonia. Currently, the most widely used real-time fluorescent quantitative RT-PCR technology. Generally, the two targets located on the ORF1ab and N genes of the virus are detected. The same specimen must meet the double target positive or the repeated test as single target positive, or the two specimens must meet the single target at the same time to confirm the positive of SARS-CoV-2 virus nucleic acid.
The virus transport medium developed by Desheng for the new coronavirus is inactivated and non-inactivated. The nucleic acid detection mainly uses an inactivated virus transport medium, which can instantly crack the pathogen and release the nucleic acid, and the protective agent can prevent the nucleic acid from being degraded. . By fully mixing the collected sample with the virus lysis solution and the virus nucleic acid preservation solution, the virus in the sample can be inactivated. Colleagues can effectively ensure the integrity of the virus nucleic acid in the sample. The collected sample can be transported and processed under normal temperature conditions. Long-term preservation. The saved viral RNA samples can be widely used in genetic testing, enzyme-linked immunoassay (ELISA), PCR testing, etc.
Although Desheng does not currently have the technology to produce nucleic acid detection kits, it can produce virus preservation solutions, disposable virus sampling tubes and biological buffers required for nucleic acid detection, as well as luminol and acridine as the raw materials for antigen and antibody detection. ester. Desheng is willing to contribute its part in the fight against the epidemic, and is willing to fight for the cause of human health.
Since the outbreak of the epidemic, relevant diagnostic reagent R&D and production companies have introduced RT-PCR nucleic acid detection kits for the first time. By collecting oropharyngeal swab or nasopharyngeal swab samples, the RNA of the virus is extracted and purified for qualitative or quantitative detection. "2019 -New Coronavirus". The sample collection and storage container used in this method is a "disposable virus sampling tube". The new coronavirus nucleic acid detection, disposable virus sampling tube can be said to be indispensable. Next, Desheng intends to introduce the composition and use of the virus sampling tube. About the composition and manufacturing of the virus sampling tube: 1. Sampling swab: The sampling swab directly contacts the sampling site, and the material of the sampling head is closely related to the subsequent detection. 2. Virus preservation solution: There are two main types of virus preservation solution widely used in the market. One is the improved virus maintenance solution based on the delivery medium, and the other is the improved preservation solution of nucleic acid extraction lysis solution. 3. Preservation tube: The material of the preservation tube should be selected carefully. There are data indicating that Polypropylene is related to the adsorption of nucleic acids, especially when the ion concentration of high tension is high, polyethylene plastic (Polyethylene) is better than polypropylene (Polypropylene). Easy to grasp DNA/RNA. About the use of virus sampling tube: The virus sampling tube sampling is mainly divided into oropharyngeal sampling and nasopharyngeal sampling: 1. Oropharyngeal sampling: first press the tongue with a tongue depressor, and then put the sampling swab head into the throat to wipe the bilateral pharyngeal tonsils and back wall of the pharynx, wipe the back wall of the pharynx with light force, avoid touching the tongue unit. 2. Nasopharyngeal sampling: use a swab to measure the distance from the tip of the nose to the earlobe and mark it with your fingers. Insert the sampling swab into the nasal cavity in the direction perpendicular to the nose (face). The distance of the swab should be at least half the length of the earlobe to the tip of the nose. Leave the swab in the nose for 15-30 seconds, gently rotate it 3-5 times, and withdraw the swab. It is not difficult to see from the method of use that whether it is an oropharyngeal swab or a nasopharyngeal swab, sampling is a technical task, which is difficult and easy to contaminate. The quality of the collected sample is directly related to the subsequent testing. If the viral load of the collected sample is Low, easy to cause false negatives, difficult to diagnose.
