Acridine Ester

Acridine Ester Luminescence Analysis Study on DNA Damage of Formaldehyde and Acetaldehyde Acridine ester can be used as a chemiluminescent indicator with a good intercalation structure of DNA double helix. Because low concentration of formaldehyde causes DNA breakage and damage, high concentration of formaldehyde causes DNA strand cross-linking, and acetaldehyde only causes DNA strand breakage damage. The chemiluminescence detection method for DNA break damage has the advantages of simplicity and sensitivity, and provides a simple research method for the study of environmental pollutant damage to DNA. Acridinium-labeled streptavidin Acridine ester DMAE -NHS was used to label streptavidin, one strain of TSH monoclonal antibody was labeled with biotin, and the other strain of TSH monoclonal antibody was coated on 96 micro-well plates, and the TSHBAS-CLIA method was established. Acridinium ester-labeled antibody or antigen, biotin-labeled antibody or antigen, streptavidin-coated magnetic beads with carboxyl or Tosyl (p-tosyl) functional groups; the particle size of the magnetic beads is 0.1-5um . Since biotin and streptavidin are combined in a ratio of 4:1, the acridinium ester-biotin-antigen-antibody complex is also combined with streptavidin in a 4-fold ratio, so that the luminescence signal is also enlarged. 4 times, the detection sensitivity is higher. Acridine ester-labeled antibody for rapid determination of antibody level in tuberculosis patients Using acridinium ester-labeled goat anti-human IgG antibodies, a method for the determination of IgG in human serum has been established. The labeling method has mild conditions, is simple and easy to implement, and its standard curve ranges from 7.5 to 125 ng IgG/ml. The results of the determination of antibody levels in the serum of tuberculosis patients are good. Acridine ester-labeled nucleic acid probe method can quickly detect Escherichia coli Use the nucleic acid probe method to quickly detect E. coli O157:H7. The method is to detect E. coli O157:H7 by using a specific DNA probe labeled with acridine ester. The nucleic acid probe method detects E. coli O157:H7 with high specificity and sensitivity. The minimum concentration of E. coli O157:H7 is about 106cfu/ml. The result of detection of E. coli O157:H7 is consistent with the national standard method; for O157 : H7 identification time is only 30 minutes, which is simple and quick. The conclusion is that the nucleic acid probe method can be used for the rapid detection of E. coli O157:H7.

1. Basic nature The application of acridine compounds in chemiluminescence detection has the following advantages: Low background luminescence and high signal-to-noise ratio; Less interference factors in luminescence reaction; The light release is fast and concentrated, the luminous efficiency is high, and the luminous intensity is large; It is easy to link with protein and the photon yield does not decrease after linking; The markers are stable (storable for several months at 2-8C), they have good solubility in water, are stable and are not easily hydrolyzed. The chemiluminescence of acridine compounds does not require a catalyst and can emit light in a dilute alkaline solution containing H2O2. The luminescence of the compound is flash type. After adding the luminescence starter, the emitted light intensity reaches the maximum in about 0.5s, and the half-life is about 1s. Acridine compounds are used in chemiluminescence immunoassay (CLIA). That is, the antibody or antigen is labeled with acridine salt, and HNO3+H2O2 and NaOH are used as the luminescence priming reagent. Some add Triton X-100, CTAC, Tween-20 and other surfactants to the luminescence priming reagent to enhance luminescence. Acridine compounds are commonly used in environmental analysis, immunoassay, enzyme activity analysis and labeling oligonucleotide fragments, etc., and are used clinically to determine bacteria, viruses or other disease markers. 2. Acridine ester DMAE-NHS or NSP-DMAE-NHS Acridine ester DMAE-NHS or NSP-DMAE-NHS is an ideal chemiluminescence immunoassay labeling substance with good luminescence performance. Compared with the widely used acridinium ester AE-NHS, the luminescence intensity of the acridinium ester DMAE-NHS or NSP-DMAE-NHS and antibody (Ab) conjugates is higher, and the luminescence dynamics of the two are very similar. Both are flash-type luminescence and both reach the highest at 0.4s, but the thermal stability of DMAE-NHS or NSP-DMAE-NHS is significantly higher than that of AE-NHS. AE-NHS is only stable in acidic solutions, when pH>6.3 It is easy to hydrolyze later, but DMAE-NHS or NSP-DMAE-NHS is not. For example, at room temperature, it is very stable in a PB buffer with a pH of 7.0. After 16 days, the luminescence activity is only reduced by 3.6%. Their luminescence thermal stability with protein conjugates is similar to this. The acridinium ester DMAE-NHS or NSP-DMAE-NHS is easy to label biomolecules and the luminescent activity and biological activity of the labeled compound are stable. The synthesized luminescent label is applied to chemiluminescence immunoassay with high sensitivity and other ideals. For example, the minimum detection limit of TSH is 0.007 mIu/L. Therefore, the acridinium ester DMAE-NHS or NSP-DMAE-NHS has good application value for the research of chemiluminescence analysis and chemiluminescence immunoassay. 3. Acridine Sulfonamide NSP-SA-NHS Acridine sulfonamide is a very effective

[Title] acridine ester me-dmae-nhs [English name] 2 ', 6' - demethylcarbonylphenyl 10-methyl-9-acridinecarboxylate 4 '- NHS ester triflate [molecular weight] 632.56 [Formula] c29h23f3n2o9s [appearance] yellow solid or powder [storage conditions] refrigerate at 2-8 , seal, dry and keep away from light [purpose] it can be used to mark proteins, antigens, antibodies, nucleic acids (DNA, RNA), etc. [product advantage] Purity: 98% (HPLC), good water solubility, stable process, small batch difference.

[Title]acridine salt nsp-sa [English name] (tosyl) carbamoyl) acridinium-10-yl) propane-1-sulfonate (nsp-sa) [CAS#]211106-69-3 [molecular weight] 584.66 [Formula] c28h28n2o8s2 [appearance] yellow solid or powder [storage conditions] refrigerate at 2-8 , seal, dry and keep away from light [purpose] it can be used to mark proteins, antigens, antibodies, nucleic acids (DNA, RNA), etc. The dilute solution of acridine and its salts shows purple or green fluorescence, while the dilute solution of salts has green fluorescence. After dilution, it becomes free acridine due to saltwater hydrolysis, showing purple fluorescence. Acridine salt is widely used as photocatalyst and dye preparation. [product advantage] Purity: 98% (HPLC), good water solubility, stable process, small batch difference.

[Title] acridine salt nsp-sa-nhs [English name] 3-[9-(((3-(N-succinimidyloxycarboxypropyl)[4-methxylphenyl]sulfonyl)amine)carboxyl]-10-acridiniumyl)-1-propanesulfonatei CAS#199293-83-9 [molecular weight] 681.73 [Formula] c32h31n3o10s2 [appearance] yellow solid or powder [storage conditions] refrigerate at 2-8 , seal, dry and keep away from light [purpose] it can be used to mark proteins, antigens, antibodies, nucleic acids (DNA, RNA), etc. [product advantage] Purity: 98% (HPLC), good water solubility, stable process, small batch difference. The related compounds have been proved to be very advantageous chemiluminescence markers, with stability, activity and sensitivity higher than those of radioisotopes.

Acridine ester (NSP-DMAE-NHS), yellow powder, CAS number: 194357-64-7, is an important chemiluminescence reagent, its chemiluminescence quantum yield is higher than luminol, and it is labeled with acridine ester The conditions are mild, the labeling rate is high, and the separation is not affected after labeling. In addition, the chemiluminescence process of acridinium ester has a rapid reaction and low background, which can emit light in the presence of sodium hydroxide and hydrogen peroxide. During the oxidation reaction, the conjugate is decomposed and does not affect the luminescence of free acridine esters; in addition, acridine esters chemiluminescent reagents have good stability and are easy to store. In addition to its application in immunoassays, acridinium esters can also be used to label oligonucleotide fragment probes for genetic or microbial assays. Acridine ester compounds are very suitable for labeling DNA strands to produce chemiluminescent DNA probes. Modern medical research results show that many diseases such as cancer and genetic diseases are related to DNA mutations, and many infectious diseases are caused by viruses, bacteria or parasites in the environment. The analysis of virus specific sequence DNA is beneficial Control of the epidemic. The detection of DNA sequence and base mutation in its chain is of far-reaching significance in gene screening, early diagnosis and treatment of genetic diseases, and pathogenic gene determination. In nucleic acid hybridization analysis, the preparation of labeled probes with strong specificity and high sensitivity is the key to successful nucleic acid hybridization analysis. Acridinium ester derivatives can be directly labeled on nucleic acid probes without the need for catalysts and the luminescence quantum yield is not affected. In addition, under certain conditions, the labeled acridinium ester on the unhybridized single-stranded DNA is hydrolyzed and destroyed, and only the double-stranded protected acridinium ester formed by hybridization can produce chemiluminescence, and the entire hybridization process can be monitored without separation. Desheng Technology has been developing chemiluminescence reagents for 12 years. After successful development, it has been put into the market and has been widely recognized by customers. The acridinium esters developed and produced have 6 different groups of products. In addition to the acridinium esters, chemiluminescent reagents also include luminol and isoluminol. The luminous efficiency of the acridine ester series is relatively high and belongs to direct luminescence.

Something about acridinium ester (NSP-SA-NHS) Acridine esters (NSP-SA-NHS) CAS: 199293-83-9 and related compounds have been proven to be very advantageous chemiluminescent labels, with stability, activity and sensitivity exceeding those of radioactive isotopes. Under alkaline conditions, the NHS will leave and the acridine ester will bind to the protein with a stable amide bond to form an acridine compound. After the reaction is completed, the excess acridine salt is removed through a desalting column. In the presence of alkaline hydrogen peroxide, acridine-labeled protein does not require enzymatic catalysis to emit light on its own. After adding the excitation reagent, the system immediately releases photons, which can be detected with a 430nm standard photometer. This light-emitting process is very short (the entire process occurs in less than 2 seconds), and the trigger scheme must add an internal photometer and photon detector. Proteins, peptides, antibodies, and nucleic acids can all be labeled with acridine. The labeled compound emits light rapidly under the excitation of basic hydrogen peroxide, and the labeled compound can be detected by collecting photons. Product stability: 1. It is stable in acidic solution (pH4.8, especially in alkaline solution, the acridine compound is partially hydrolyzed to reduce its stability, and the hydrolysis process is a dark reaction process that does not emit light; the degree of hydrolysis increases with the increase of pH, and the degree of hydrolysis increases with the increase of temperature. Increase and increase. 3. The stability of acridine amide is higher than that of acridine ester structure, and the ability to resist hydrolysis is stronger. 4. Acridine ring or phenol ring or benzene sulfonyl ring is connected with a methyl group and other electricity-donating groups. Due to the large steric hindrance, the thermal stability is increased; the electron withdrawing group is attached, which is beneficial to the nucleophilic substitution reaction Its stability decreases. 5. Suggestion: Use a weakly acidic buffer as much as possible for the storage buffer after coupling, and bubbling nitrogen to remove oxygen. Store at low temperature, sealed and protected from light. Conditions can be made into freeze-dried samples and then stored. Precautions: 5. The acridinium ester luminescence process is very short (the whole process takes less than 2 seconds), and the trigger scheme must add an internal photometer and photon detector; in addition, this product can also use a multifunctional microplate reader equipped with an autosampler Perform luminescence data collection to minimize the influence of sample addition time on the collected signal.