Serum separation gel, a kind of raw material commonly used in hospital laboratory, is inseparable from many biochemical analyses. It is an inert polymer, insoluble in water, and has high temperature resistance, low temperature resistance, oxidation resistance, and high stability. Some manufacturers such as Desheng's separation glue also have the characteristics of anti-radiation.
With the improvement of peopleâ??s health awareness, blood testing has become more common. Serum separation gel is favored by various medical institutions. There are many manufacturers of separation gel. Due to the different requirements of medical institutions for separation gel, each manufacturer produces The components of the separation rubber are different, regardless of the transparency, color, viscosity, specific gravity and other aspects of the performance. The high-quality serum separation gel can shorten the serum separation time, and the separation gel is between the serum and blood cells, which can effectively protect the serum components, so as to realize the original tube on the machine and the original tube to save the serum for future reference, reducing the possibility of errors caused by re-transfer Sex. However, the impact of inferior separation gel on the test items cannot be ignored. The use of inferior separation gel detection can increase the triglyceride (TG) in the test results abnormally. Therefore, a comparison test must be done before using serum separation gel. The following is a quick, easy and easy-to-operate method to detect triglycerides in the separation gums of various manufacturers, as follows:
1. Apparatus and reagents: automatic biochemical analyzer, triglyceride (TRIG) reagent and calibration solution are special reagents for the instrument, 5ml disposable sterile syringe, Desheng serum separation gel blood collection tube, a certain brand of inferior serum Separation gel blood collection tube, traditional ordinary blood collection tube, 0.9% sodium chloride injection.
2. Method: The traditional ordinary blood collection tube is the control group A, the Desheng serum separation gel collection tube is the control group B, and a certain brand of inferior serum separation gel collection tube is the experimental group C. Each group randomly selects 10 blood collection tubes, each tube Add 5ml 0.9% sodium chloride injection separately, place in 37â?? water bath for 15min, centrifuge at 3000r/min for 10 minutes, measure the above tubes on the automatic biochemical analyzer, record the test results, and compare the results It can be found that TG cannot be detected in the blood collection tubes of group A and B, but different concentrations of TG can be detected in each tube of group C. This method can be used to conduct a comparison test to quickly and accurately identify the quality of the separation gel in the blood collection tube The pros and cons.