Acridine Ester Luminescence Analysis Study on DNA Damage of Formaldehyde and Acetaldehyde
Acridine ester can be used as a chemiluminescent indicator with a good intercalation structure of DNA double helix. Because low concentration of formaldehyde causes DNA breakage and damage, high concentration of formaldehyde causes DNA strand cross-linking, and acetaldehyde only causes DNA strand breakage damage. The chemiluminescence detection method for DNA break damage has the advantages of simplicity and sensitivity, and provides a simple research method for the study of environmental pollutant damage to DNA.
Acridinium-labeled streptavidin
Acridine ester DMAE -NHS was used to label streptavidin, one strain of TSH monoclonal antibody was labeled with biotin, and the other strain of TSH monoclonal antibody was coated on 96 micro-well plates, and the TSHBAS-CLIA method was established. Acridinium ester-labeled antibody or antigen, biotin-labeled antibody or antigen, streptavidin-coated magnetic beads with carboxyl or Tosyl (p-tosyl) functional groups; the particle size of the magnetic beads is 0.1-5um . Since biotin and streptavidin are combined in a ratio of 4:1, the acridinium ester-biotin-antigen-antibody complex is also combined with streptavidin in a 4-fold ratio, so that the luminescence signal is also enlarged. 4 times, the detection sensitivity is higher.
Acridine ester-labeled antibody for rapid determination of antibody level in tuberculosis patients
Using acridinium ester-labeled goat anti-human IgG antibodies, a method for the determination of IgG in human serum has been established. The labeling method has mild conditions, is simple and easy to implement, and its standard curve ranges from 7.5 to 125 ng IgG/ml. The results of the determination of antibody levels in the serum of tuberculosis patients are good.
Acridine ester-labeled nucleic acid probe method can quickly detect Escherichia coli
Use the nucleic acid probe method to quickly detect E. coli O157:H7. The method is to detect E. coli O157:H7 by using a specific DNA probe labeled with acridine ester. The nucleic acid probe method detects E. coli O157:H7 with high specificity and sensitivity. The minimum concentration of E. coli O157:H7 is about 106cfu/ml. The result of detection of E. coli O157:H7 is consistent with the national standard method; for O157 : H7 identification time is only 30 minutes, which is simple and quick. The conclusion is that the nucleic acid probe method can be used for the rapid detection of E. coli O157:H7.
Sugar Esters E473, CAS No.37318-31-3 , commonly known as Sucrose esters of fatty acids, manufacturing process by esterifying sucrose with fatty acids. By varying the degree of esterification of the sucrose molecule it is possible to obtain emulsifiers with HLB values ranging from 1 up to 16. It is a light yellow powder, a non-ionic surfactant and is used as an emulsifier in dairy products, baked goods, soft drinks, jelly, cake and candy. Sucrose Fatty Acid Esters are a mixture of mono-, di-, and tri-esters of sucrose with fatty acids derived from edible vegetable or hydrogenated vegetable oils and fats. As an experienced Sugar Esters manufacturer and supplier, we has been supplying and exporting Sugar Esters for almost 10 years, please be assured to buy from us. Any inquiries about price and the market trend please feel free to contact us, we will reply you within 1 working day. Sugar Esters powder Specification ITEM STANDARD RESULTS APPERANCE White crystal powder Conforms ACID VALUE (AS KOHMG/G) Not more than 6 3.0 ARSENIC (AS)MG/KG Not more than Less than 1.0 LEAD (PB) MG/KG Not more than Less than 2.0 CADMIUM MG/KG Not more than Less than1 MERCURY MG/KG Not more than Less than1 OTHER SOLVENT Isopropy 350ppm(incombination) Pass Ethyl acetate 126.7-128C Propylene glycol Pass Isobutyl alcohol 10ppm Pass Methy alcohol 10ppm Pass Methyl Ethyl Ketone 10ppm Pass DIMETHYL SULFOXIDE sulfoxide Not more than 2ppm Pass FREE SUGER(AS SUGER) % Not more that 5% 1.35 DIMETHYLFORMAMIDE Not more than 1ppm Pass WATERCONTENT% Not more than 4% 0.51 LOSS ON IGNITION % Not more that 4% 0.47 HLB VALUE 13
Desheng is a new scientific and technological enterprise specializing in the research and development, production and sales of blood collection additives, chemiluminescence reagents, biological buffers and in vitro diagnostic reagents. Acridine ester series of products are the company's research and development of ultra-high sensitivity chemiluminescence substrate reagents. Under alkaline conditions, the acridine ester molecules can be attacked by hydrogen peroxide to form dioxane, which is unstable and decomposes into CO2 and n-methylacridone in the electron excited state. When it returns to the ground state, it emits light with wavelength of 430 nm. It does not need catalyst, and the luminescence system is simple. Desheng has seven acridine ester products with different groups: 1. Ae-nhs (traditional acridine ester); 2. Dmae-nhs; 3. Me-dmae-nhs; 4. Nsp-dmae-nhs; 5. Nsp-sa-nhs; 6. Nsp-sa; 7. Nsp-sa-adh. These acridine esters produced by Desheng have the characteristics of high purity and small difference between batches. Besides, it provides other immune reagents for matching use and provides a complete set of technical services and technical support.
1. Basic nature The application of acridine compounds in chemiluminescence detection has the following advantages: Low background luminescence and high signal-to-noise ratio; Less interference factors in luminescence reaction; The light release is fast and concentrated, the luminous efficiency is high, and the luminous intensity is large; It is easy to link with protein and the photon yield does not decrease after linking; The markers are stable (storable for several months at 2-8C), they have good solubility in water, are stable and are not easily hydrolyzed. The chemiluminescence of acridine compounds does not require a catalyst and can emit light in a dilute alkaline solution containing H2O2. The luminescence of the compound is flash type. After adding the luminescence starter, the emitted light intensity reaches the maximum in about 0.5s, and the half-life is about 1s. Acridine compounds are used in chemiluminescence immunoassay (CLIA). That is, the antibody or antigen is labeled with acridine salt, and HNO3+H2O2 and NaOH are used as the luminescence priming reagent. Some add Triton X-100, CTAC, Tween-20 and other surfactants to the luminescence priming reagent to enhance luminescence. Acridine compounds are commonly used in environmental analysis, immunoassay, enzyme activity analysis and labeling oligonucleotide fragments, etc., and are used clinically to determine bacteria, viruses or other disease markers. 2. Acridine ester DMAE-NHS or NSP-DMAE-NHS Acridine ester DMAE-NHS or NSP-DMAE-NHS is an ideal chemiluminescence immunoassay labeling substance with good luminescence performance. Compared with the widely used acridinium ester AE-NHS, the luminescence intensity of the acridinium ester DMAE-NHS or NSP-DMAE-NHS and antibody (Ab) conjugates is higher, and the luminescence dynamics of the two are very similar. Both are flash-type luminescence and both reach the highest at 0.4s, but the thermal stability of DMAE-NHS or NSP-DMAE-NHS is significantly higher than that of AE-NHS. AE-NHS is only stable in acidic solutions, when pH>6.3 It is easy to hydrolyze later, but DMAE-NHS or NSP-DMAE-NHS is not. For example, at room temperature, it is very stable in a PB buffer with a pH of 7.0. After 16 days, the luminescence activity is only reduced by 3.6%. Their luminescence thermal stability with protein conjugates is similar to this. The acridinium ester DMAE-NHS or NSP-DMAE-NHS is easy to label biomolecules and the luminescent activity and biological activity of the labeled compound are stable. The synthesized luminescent label is applied to chemiluminescence immunoassay with high sensitivity and other ideals. For example, the minimum detection limit of TSH is 0.007 mIu/L. Therefore, the acridinium ester DMAE-NHS or NSP-DMAE-NHS has good application value for the research of chemiluminescence analysis and chemiluminescence immunoassay. 3. Acridine Sulfonamide NSP-SA-NHS Acridine sulfonamide is a very effective
[Title] acridine ester me-dmae-nhs [English name] 2 ', 6' - demethylcarbonylphenyl 10-methyl-9-acridinecarboxylate 4 '- NHS ester triflate [molecular weight] 632.56 [Formula] c29h23f3n2o9s [appearance] yellow solid or powder [storage conditions] refrigerate at 2-8 , seal, dry and keep away from light [purpose] it can be used to mark proteins, antigens, antibodies, nucleic acids (DNA, RNA), etc. [product advantage] Purity: 98% (HPLC), good water solubility, stable process, small batch difference.
Ferulic acid has a high content in traditional Chinese medicines such as Ferulic acid Angelica sinensis Chuanxiong Cimicifuga and Suanzaoren One is obtained from the combination of ferulic acid and some small molecules the second is obtained from the plant cell wall and the third is obtained through tissue culture Ferulic acid in plants is often crosslinked with polysaccharides and lignin through ester bonds or selfesterified or etherified to form di ferulic acid Generally alkaline and enzymatic methods are used to break the ester bonds to release ferulic acid and then suitable solvents are used for extraction Item Specifications Results Appearance White or lightyellow acicular crystal Passed Melting point 172 01760C 17261748C Loss on drying 050 016 Residue on ignition 010 004 Heavy metals 10PPM Passed Unspecified impurities 010 Not detected Total 050 Not detected Contents 990 1000 Conclusion Conforms
Product Name: Isopropyl palmitate Synonyms: kesscoipp;kesscoisopropylpalmitate;Lexol IPP;Liponate IPP;nikkolipp;Palmitic acid esters;Plymouth ipp;plymouthipp CAS: 142-91-6 MF: C19H38O2 MW: 298.5 EINECS: 205-571-1 Product Categories: Bioactive Small Molecules;Building Blocks;C12 to C63;Carbonyl Compounds;Cell Biology;Chemical Synthesis;Hair Care;Skin Care;Esters;I;Organic Building Blocks;Cosmetic raw material;142-91-6 Mol File: 142-91-6.mol Melting point 11-13 �°C (lit.) Boiling point 160�°C 2mm density 0.852 g/mL at 25 �°C (lit.) vapor pressure 0.007Pa at 25�ºC refractive index n20/D 1.438(lit.) Fp >230 �°F storage temp. 2-8�°C solubility
2-Ethylhexyl Salicylate, or octyl salicylate, is an organic compound used as an ingredient in sunscreens and cosmetics to absorb UVB (ultraviolet) rays from the sun. It is an ester formed by the condensation of salicylic acid with 2-ethylhexanol. It is a colorless oily liquid with a slight floral odor. The salicylate portion of the molecule absorbs ultraviolet light, protecting skin from the harmful effects of exposure to sunlight. The ethylhexanol portion is a fatty alcohol, adding emollient and oil-like (water resistant) properties. Test Items Specifications Results Appearance Colorless to pale yellow liquid Colorless liquid Odour Slight characteristic Slight characteristic Identity (IR) USP Matches reference spectrum Matches reference spectrum Identity (Retention time) USP Matches reference retention time Matches reference retention time UV Specific extinction (E1%1cm,310nm, ethanol) USP 165-185 175 Specific gravity(25ºC) USP 1.011-1.016 1.015 Refractive index(20ºC) USP 1.500-1.503 1.503 Acidity(ml/ml) Titration (USP) 0.2 0.0ml/ml Chromatographic purity GC USP Purity (%) 99.0 99.6 Ethylhexanol(%) 0.1
[Title]acridine salt nsp-sa [English name] (tosyl) carbamoyl) acridinium-10-yl) propane-1-sulfonate (nsp-sa) [CAS#]211106-69-3 [molecular weight] 584.66 [Formula] c28h28n2o8s2 [appearance] yellow solid or powder [storage conditions] refrigerate at 2-8 , seal, dry and keep away from light [purpose] it can be used to mark proteins, antigens, antibodies, nucleic acids (DNA, RNA), etc. The dilute solution of acridine and its salts shows purple or green fluorescence, while the dilute solution of salts has green fluorescence. After dilution, it becomes free acridine due to saltwater hydrolysis, showing purple fluorescence. Acridine salt is widely used as photocatalyst and dye preparation. [product advantage] Purity: 98% (HPLC), good water solubility, stable process, small batch difference.
[Title] acridine salt nsp-sa-nhs [English name] 3-[9-(((3-(N-succinimidyloxycarboxypropyl)[4-methxylphenyl]sulfonyl)amine)carboxyl]-10-acridiniumyl)-1-propanesulfonatei CAS#199293-83-9 [molecular weight] 681.73 [Formula] c32h31n3o10s2 [appearance] yellow solid or powder [storage conditions] refrigerate at 2-8 , seal, dry and keep away from light [purpose] it can be used to mark proteins, antigens, antibodies, nucleic acids (DNA, RNA), etc. [product advantage] Purity: 98% (HPLC), good water solubility, stable process, small batch difference. The related compounds have been proved to be very advantageous chemiluminescence markers, with stability, activity and sensitivity higher than those of radioisotopes.
