Introduction:
4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells.
Application:
HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile.
Buffer solution preparation:
(1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L.
(2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4�ºC or -20�ºC.
Purpose:
1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA
2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase
3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer
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Applications: In Animal feed stuff Nutrition feed for Poultry, Layers, Cattle. Primarily used as a dairy cow feed supplement, the buffering capability of Natural Soda's pure and natural feed grade sodium bicarbonate helps stabilize rumen pH by reducing acidic conditions. Improves calcium absorption and eggshell quality of laying hens during peak production.
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Formula:KAl(SO4)2 12H2O Relative Molecular Weight:474.39 CAS NO,: 7784-24-9 EINECS No.:5637-689-7 HS CODE.: 2833301000 Appearance: lump, white crystal, white powder Properties:Colorless, transparent, particle or crystalline powder, odorless, nontoxic, tastes slightly sweet and puckery, astringent, easily soluble in water, its water solution appears acid,is would lose crystal water and become white powder on heating Usage; Food industry: Buffering agents and leavening agents in food-grade products. Chemical Industry: Water Flocculant, aluminum tanning agents and after-treating agents for leather tanning in the leather industry, dyeing agents,Astringents,mordants etc. Items Specifications Potassium aluminum sulfate [AlK(SO4)2.12H2O] % 99.5 Plumbum(Pb) mg/kg 5.0 Arsenic (As) mg/kg 2.0 Fluorine (F) mg/kg 30.0 Selenium (Se) mg/kg 30.0 Moisture % 4.0
Monocalcium Phosphate (MCP) Feed grade Product Features The purity of the product, calcium and phosphorus ratio appropriate to ensure a higher degree of its biological effects. Phosphorus content greater than 22% of the products, their biological effects better. This unique high-tech companies use technology to make our products crystallization, high purity and its harmful impurities significantly below standards:HG28611997 Drying process advanced products, products with low free water under the premise that the product does not lose crystallization of water, avoid the product in the drying process for the loss of water caused by crystallization of biological effects of the reduction. Specifications Appearance white powder or granule Total P 22%min Ca 13-18% F 0.18% max As 10 ppm max Pb 10ppm max Moisture 4%max Source of OriginRock Packing and Storage Knitted bags lined polyethylene plastic bags double packaging, Weighing 25Kg. Transportation-and harsh rain. Stored in a cool, ventilated, dry place
Product Features The purity of the product, calcium and phosphorus ratio appropriate to ensure a higher degree of its biological effects. Phosphorus content greater than 21% of the products, their biological effects better. This unique high-tech companies use technology to make our products crystallization, high purity. Drying process advanced products, products with low free water under the premise that the product does not lose crystallization of water, avoid the product in the drying process for the loss of water caused by crystallization of biological effects of the reduction. coarse particles MDCP in the animals stay in the stomach longer and more conducive to the absorption of calcium phosphate, the product produced by micro-particles MDCP form, without any chemical binder. Specifications Appearance white powder or granule Phosphate  21% min Calcium     15% min Fluorine    0.18% max Arsenic    30ppm max Pb   30ppm max Source of OriginRock Packing and Storage Knitted bags lined polyethylene plastic bags double packaging, Weighing 25Kg. Transportation-and harsh rain. Stored in a cool, ventilated, dry place.
Aluminium tri-sec-butoxide Product Name: Aluminium tri-sec-butoxide CAS: 2269-22-9 MF: C12H27AlO3 MW: 246.32 EINECS: 218-871-2 Mol File: 2269-22-9.mol Chemical Properties Boiling point 40 C density 0.967 g/mL at 25 C(lit.) vapor pressure 23 hPa (195 C) refractive index 1.439 Fp 82 F storage temp. Flammables area solubility Miscible with alcohol, isopropyl alcohol and toluene. form Oily Liquid Specific Gravity 0.9671 color Light yellow Item Specifications Appearance Colorless transparent or light yellow liquid Purity % 99.99 Aluminum content % 10.2-12.5 Density ( g/cm3) 0.92-0.97
Commodity: Dicalcium phosphate Feed Grade Use DCP is the feed preparation of raw materials, Animal right to supplement calcium needs. Coarse its products more applicable to the preparation hens, ducks feed, concentrate feed and premix. Product Nature Molecular Formula:CaHPO4·2H2O,Molecular weight:172.09,After drying with loose white powdery crystals or granules. Neutral products, the melting acid, water-soluble, hygroscopicity small, heated to 90 around gradually lose water of crystallization. Product Features The purity of the product, calcium and phosphorus ratio appropriate to ensure a higher degree of its biological effects. Phosphorus content greater than 18% of the products, due to a small amount of MCP, and its biological effects better. This unique high-tech companies use technology to make our products crystallization, high purity and its harmful impurities significantly below standards:HG26362000 Drying process advanced products, products with low free water under the premise that the product does not lose crystallization of water, avoid the product in the drying process for the loss of water caused by crystallization of biological effects of the reduction. Coarse particle DCP in the animals stay in the stomach longer and more conducive to the absorption of calcium phosphate, the product of Micro-particle DCP granulation from mechanical, without any chemical binder. Specifications Appearance white and gray powder/granule Total P 18% min Calcium 21% min F 0.18% max As 30 ppm max Pb 30 ppm max Packing and Storage Knitted bags lined polyethylene plastic bags double packaging, Weighing 25Kg/50KG/1000Kg. Transportation-and harsh rain. Stored in a cool, ventilated, dry place.
