Introduction:
4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells.
Application:
HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile.
Buffer solution preparation:
(1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L.
(2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4�ºC or -20�ºC.
Purpose:
1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA
2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase
3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer
1 27176-87-0 Dodecylbenzenesulphonic acid 2 151-21-3 Sodium dodecyl sulfate 3 9016-45-9 Nonylphenol Ethoxylate 4 63449-41-2 Benzalkonium Chloride 5 139-07-1 Dodecyldimethylbenzylammonium chloride 6 9004-82-4 AES 7 6863-42-9 Coconut oil acid diethanolamine 8 683-10-3 Lauryl betaine 9 25155-30-0 Sodium dodecyl benzene sulfonate 10 107-43-7 Betaine 11 112-00-5 Dodecyltrimethylammonium chloride 12 25496-72-4 Glyceryl monooleate 13 1120-02-1 Octadecy trimethyl ammonium bromide 14 9005-64-5 Polysorbate 20 15 9005-67-8 Tween 60 16 68213-23-0 Fatty alcohol polyglycol ether 17 68585-34-2 Sodium lauryl ether sulfate 18 9004-95-9 Polyethylene Glycol Monocetyl Ether 19 9004-98-2 Polyethylene glycol monooleyl ether 20 9003-11-6 Polyethylene-polypropylene glycol
S.N CAS No. Item 1 100-21-0 Terephthalic acid 2 67763-03-5 Silsesquioxanes 3 9003-01-4 Acrylic acid 4 1333-86-4 Carbon black 5 80-62-6 Methyl methacrylate 6 61788-97-4 Haloperidol 7 75-38-7 1,1-difluoroethylene 8 100-42-5 Styrene 9 9002-84-0 Poly(tetrafluoroethylene) 10 124-04-9 Adipic acid 11 107-21-1 Ethylene glycol 12 126-30-7 Neopentyl glycol 13 85-44-9 Phthalic anhydride 14 106-89-8 Epichlorohydrin 15 9003-08-1 Amino resin 16 9006-03-5 Chlorinated rubber 17 13463-67-7 Titanium dioxide 18 12227-89-3 Iron Oxide Black 19 922-67-8 Methylpropiolate 20 538-24-9 Trilaurin 21 9011-05-6 Urea formaldehyde 22 79-41-4 Methacrylic acid 23 1314-13-2 Zinc oxide 24 80-05-7 Bisphenol A 25 121-91-5 Isophthalic acid
Applications: Food ingredients for bakery, beverage, confectionery industry It is used as a raising agent in biscuits, cookies, bread, cakes, farsan, jaggery & confectionery. It acts like a buffer and carbonation agent in beverages, electrolyte and rehydration solutions.
1 141-78-6 Ethyl acetate 2 79-20-9 Methyl Acetate 3 123-86-4 Butyl acetate 4 108-32-7 Propylene carbonate 5 75-12-7 Formamide 6 127-19-5 N,N-dimethylacetamide 7 872-50-4 N-methylpyrrolidin-2-one 8 26171-83-5 1,2-butanediol 9 504-63-2 1,3-Propanediol 10 26761-45-5 2,3-Epoxypropyl neodecanoate 11 57-55-6 Propylene Glycol 12 108-94-1 Cyclohexanone 13 95-47-6 o-xylene 14 2238-07-5 Diglycidyl ether 15 122-60-1 Phenyl glycidyl ether 16 34590-94-8 Dipropylene glycol monomethyl ether 17 110-54-3 Hexane 18 109-99-9 Tetrahydrofuran 19 75-09-2 Dichloromethane 20 110-82-7 Cyclohexane
Applications: In Animal feed stuff Nutrition feed for Poultry, Layers, Cattle. Primarily used as a dairy cow feed supplement, the buffering capability of Natural Soda's pure and natural feed grade sodium bicarbonate helps stabilize rumen pH by reducing acidic conditions. Improves calcium absorption and eggshell quality of laying hens during peak production.
1 108-78-1 Melamine 2 37640-57-6 Melamine cyanurate 3 63428-83-1 Polyamide 4 106-50-3 1,4-phenylenediamine 5 9002-89-5 Poly(vinyl alcohol) 6 105-60-2 �µ-caprolactam 7 10578-34-4 Stearyl benzoate 8 108-31-6 Maleic anhydride 9 108-05-4 Vinyl acetate 10 26264-06-2 Calcium dodecylbenzene sulfonate 11 111-69-3 Adiponitrile 12 924-42-5 N-Methylolacrylamide 13 100-20-9 Terephthaloyl Chloride 14 99-63-8 Isophthaloyl Chloride 15 1338-02-9 Copper naphthenate 16 6153-56-6 Oxalic acid dihydrate 17 79-10-7 Acrylic acid 18 75-01-4 Chloroethene 19 25190-06-1 Poly(butylene oxide) macromolecule 20 77-99-6 Trimethylol propane
1 101-68-8 4,4'-Diphenylmethane diisocyanate 2 9016-00-6 Poly(dimethylsiloxane) 3 26471-62-5 Tolylene diisocyanate 4 100-42-5 Styrene 5 141-32-2 Butyl acrylate 6 107-13-1 Acrylonitrile 7 106-99-0 Buta-1,3-diene 8 75-38-7 1,1-difluoroethylene 9 9-38-9 Chlorotrifluoroethylene 10 111-44-4 2,2'-Dichlorodiethyl ether 11 1187-93-5 Trifluoromethyl trifluorovinyl ether 12 21645-51-2 Aluminum hydroxide 13 116-14-3 Tetrafluoroethene 14 116-15-4 Hexafluoropropylene 15 126-99-8 Chloroprene 16 103-11-7 2-Ethylhexyl acrylate 17 140-88-5 Ethyl acrylate 18 78-79-5 Isoprene 19 96-33-3 Methyl acrylate 20 1300-21-6 Dichloroethane
1 117-81-7 Bis(2-ethylhexyl) phthalate 2 117-84-0 Di-n-octyl phthalate 3 85-69-8 Butyl 2-ethylhylhexyl phthalate 4 84-61-7 Dicyclohexyl phthalate 5 84-74-2 Dibutyl phthalate 6 84-69-5 Diisobutyl phthalate 7 131-11-3 Dimethyl phthalate 8 84-66-2 Diethyl phthalate 9 20548-62-3 Bis(7-methyloctyl) phthalate 10 68515-49-1 Diisodecyl phthalate 11 84-75-3 Di-n-hexyl phthalate 12 6422-86-2 Dioctyl terephthalate 13 137-89-3 Bis(2-ethylhexyl) isophthalate 14 123-79-5 Dioctyl adipate 15 122-62-3 Bis(2-ethylhexyl)sebacate 16 109-43-3 Bis(n-butyl)sebacate 17 512-56-1 Ttrimethyl phosphate 18 126-73-8 Tributyl phosphate 19 8013-07-8 Epoxidized soya bean oil 20 77-93-0 Triethyl citrate
Formula:KAl(SO4)2 12H2O Relative Molecular Weight:474.39 CAS NO,: 7784-24-9 EINECS No.:5637-689-7 HS CODE.: 2833301000 Appearance: lump, white crystal, white powder Properties:Colorless, transparent, particle or crystalline powder, odorless, nontoxic, tastes slightly sweet and puckery, astringent, easily soluble in water, its water solution appears acid,is would lose crystal water and become white powder on heating Usage; Food industry: Buffering agents and leavening agents in food-grade products. Chemical Industry: Water Flocculant, aluminum tanning agents and after-treating agents for leather tanning in the leather industry, dyeing agents,Astringents,mordants etc. Items Specifications Potassium aluminum sulfate [AlK(SO4)2.12H2O] % 99.5 Plumbum(Pb) mg/kg 5.0 Arsenic (As) mg/kg 2.0 Fluorine (F) mg/kg 30.0 Selenium (Se) mg/kg 30.0 Moisture % 4.0
