Luminol / 3-Aminobenzoylhydrazide

Luminol can be oxidized by many substances to produce chemiluminescence, which is the earliest research and most widely used luminescence reagent. The chemiluminescence analysis of metal ions occupies an important position in the entire research and application of chemiluminescence analysis. Some metal ions can enhance the chemiluminescence of the luminol system; some ions can inhibit the chemiluminescence of the luminol system; and some ions can directly oxidize the luminol to produce chemiluminescence. In our drug analysis, we use the catalysis or inhibition of metal ions and compounds for direct or indirect detection. Let's take a look at how luminol "extraordinarily" in drug analysis. Application of Luminol Luminescence System in Pharmaceutical Analysis 1. Direct or indirect detection using the catalysis or inhibition of metal ions and metal ion compounds, such as the determination of sodium nitroprusside (SNP) and catechol compounds. Certain reducing substances such as citric acid, citrate esters, Vc, ascorbic acid, etc. can also be detected by the metal ion-luminol luminescence system. 2. The enhancement or inhibition of the chemiluminescence reaction of luminol by biomolecules and organic substances. DNA, albumin, glucose, and uric acid can enhance the chemiluminescence reaction of luminol, thus establishing their own detection methods. In an alkaline medium, the inhibitory effect of ascorbic acid on the Luminol-K3Fe(CN)6 system can be used to determine ascorbic acid. Luminol-K3Fe(CN)6 series can also be used to monitor glucose and lactic acid. Thiol-containing drugs, promazine, etc. have an inhibitory effect on the luminol luminescence system, and a detection method is proposed accordingly. 3. Preparation of expendable chemiluminescence sensor. Luminol and other reagents are fixed on ion exchange resin to develop a chemiluminescence flow injection sensor, which is used for the determination of Vc. Combine enzyme immobilized column with FIA, use immobilized enzyme reactor, detect by luminol, it has been used for glucose, alcohol, oxalate, lactate, choline, uric acid in serum and urine, etc. Detection. Seeing more of the power of luminol, we suddenly discovered that it is acceptable to us wherever it is. This also fully illustrates its value. If you want to know more about luminol's knowledge and uses, you might as well find its manufacturer-Desheng. I believe you will be worthy of asking.

Luminol, is a yellow crystal or beige powder at room temperature and is a relatively stable chemical reagent. At the same time, luminol is a strong acid, which has a certain irritating effect on eyes, skin and respiratory tract. It is one of the oldest and most commonly used reagents. It can be oxidized by peroxide under alkaline conditions and emit light at the same time. The redox reaction between luminol and peroxide requires a catalyst, which is generally more Valence metal ions, peroxidases such as iron, horseradish peroxidase, etc. This method is often used to detect the content of peroxides, heavy metals, peroxidase, and the derived free radicals, for toxicant analysis And analysis methods based on peroxidase and glucose oxidase. Under normal circumstances, the chemiluminescence reaction between luminol and hydrogen peroxide is very rapid in the presence of certain catalysts. The most commonly used catalyst is metal ions. In a large concentration range, the concentration of metal ions is directly proportional to the luminescence intensity, so that chemiluminescence analysis of certain metal ions can be performed. This reaction can be used to analyze organic compounds containing metal ions to achieve Very high sensitivity. The second is to use the inhibitory effect of organic compounds on the chemiluminescence reaction of luminol to determine the organic compounds that have a quenching effect on the chemiluminescence reaction. The third is the indirect determination of inorganic or organic compounds through coupling reactions. Luminol Luminescence Principle One is that sodium hypochlorite oxidizes luminol to make it glow; The second is that hydrogen peroxide reacts with sodium hypochlorite to generate oxygen and oxidize luminol to make it glow: The first is the equation for the reaction of sodium hypochlorite and hydrogen peroxide: NaClO+ H2O2== NaCl + O2+ H2O Secondly, when luminol reacts with hydroxide, a double negative ion (Dianion) is formed, which can be oxidized by oxygen generated by the decomposition of hydrogen peroxide, and the product is an organic peroxide. The peroxide is very unstable and immediately decomposes nitrogen (Luminol is not oxidized by organic oxidants such as dimethyl sulfoxide to generate nitrogen, but to generate nitrogen-containing organics) to generate excited 3-aminophthalic acid . In the transition from the excited state to the ground state, the released energy exists in the form of photons with a wavelength in the blue part of visible light. The method of indirect determination by coupling refers to the combination of one reaction that can produce or consume chemiluminescence reactants with another chemiluminescence reaction, so that indirect chemiluminescence determination of certain substances can be achieved. This method is used when determining the purity of certain substrate enzymes.

There are a few points to pay attention to when using ECL luminescent liquid: 1. When using ECL, the previous steps can be carried out in sunlight, but the sensitivity may be slightly reduced when exposed to strong light for too long. Wearing gloves can avoid leaving fingerprints on the film. 2. If the exposure is too long or the protein is too much, the background will deepen and the band strength will lose the linear relationship, while the underexposure will blur the band. 3. The strips on the membrane glow after incubating for about 1 minute in the luminescent working solution. The strong band luminescence is visible to the naked eye in the darkroom, and the low-abundance protein band luminescence is weak or even invisible to the naked eye, but it can be exposed to X-ray film. The light emission time of the strip cannot be judged by visual observation alone. 4. Too high antibody concentration will cause high background or no bands, which will lead to failure! ECL luminescent fluid is mainly made of luminol, isoluminol or isoluminol derivatives, sodium hydroxide and hydrogen peroxide. Desheng produces luminol and isoluminol raw materials, which need to be matched Lye, hydrogen peroxide, and peroxidase can complete the chemiluminescence experiment.

Supplier: Blood collection additives, raw materials for in vitro diagnostic reagents, like serum separating gel, sodium heparin, lithium heparin , blood coagulant, edta k2, edta k3, chemiluminescent reagent (3 aminophthalhydrazide, luminol, 4 aminophthalhydrazide), trinder's reagent (toos, tops, ados), cosmetic raw material (climbazole, triethanolamine)
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Usage: Used for in vitro anticoagulation of blood. It is an anticoagulant additive for clinical blood testing. The pretreatment of blood samples, that is, anticoagulation treatment, is performed during clinical blood collection and testing. Characteristics: 1. It has a professional R&D technical team for disodium EDTA; 2. Desheng is a direct manufacturer of disodium EDTA, and has long-term stock of disodium EDTA, which can be shipped immediately; 3. Desheng has many years of research and development Experience in the production of disodium EDTA, product quality is excellent;

Usage: Sodium fluoride is an inorganic compound, CAS7681-49-4, which is mainly used in blood collection tube additives, phosphating accelerators, agricultural pesticides, sealing materials, preservatives and other fields. Characteristics: 1. It has a professional sodium fluoride R&D technical team, and can answer various questions about the use and preparation at any time; 2. Desheng is a direct manufacturer of sodium fluoride, and has long-term preparation. Sodium fluoride is available in stock and can be shipped immediately; 3. Desheng has many years of experience in R&D and production of sodium fluoride, and has professional solutions to sodium fluoride product problems; 4. Has a professional foreign trade team and rich export experience, one order can be completed without worry till receiving the goods.

Usage: Sodium citrate can be used as anticoagulation for most blood coagulation experiments. It is less toxic to cells and can also be used as one of the components of blood maintenance fluids in blood transfusion experiments. Characteristics: 1. There is a professional production and R&D team with rich product knowledge and more stable performance, which can effectively solve any problems encountered during use; 2. The source manufacturer, with high daily output and stable supply, can deliver goods immediately to meet the purchase demand; 3. With a professional foreign trade team and rich export experience, Desheng's service follows the customer-oriented service. Good customer service is the highest standard of Desheng people. It can effectively communicate and solve the product problems of sodium citrate, and can provide perfect after-sales service; 4. The purity of Desheng sodium citrate products is as high as 99 or more, and all indicators meet the requirements of the highest international standards.

Usage: Potassium oxalate is an organic potassium salt with a molecular formula of K2C2O4. It is often used in conjunction with sodium fluoride as a special tube for blood sugar testing, for testing blood sugar, glucose tolerance, and anti-alkali blood protein. Desheng provides high-quality potassium oxalate products to meet the needs of blood collection tube manufacturers. Characteristics: 1. Desheng has 18 years of experience in R&D and production of blood collection tube additives. It is relatively clear about the use of potassium oxalate, and can answer other problems that may arise with potassium oxalate very well and professionally; 2. Desheng is a direct manufacturer of potassium oxalate in China. It has stock in stock for a long time. Orders can be delivered quickly, and intermediate links are eliminated, saving you costs; 3. Desheng has a professional and experienced foreign trade team, which can seamlessly connect with any questions you have in order, use and follow-up, without any worries.

Usage: Used in blood collection tubes. It is a more advanced high-efficiency silicifying agent that Desheng developed after water-soluble silicifying agent. The silicification effect is more prominent than that of the water-soluble silicifying agent. Characteristics: 1. Desheng oil-based silicifying agent is a high-efficiency silicifying agent with more advanced silicification effects than water-based one. Whether it is plastic, glass or other materials, it can play a good role in silicification. And for the use of problems that arise, we can give professional responses in a timely manner; 2. Desheng is a direct manufacturer of oil-based silicifying agents. The products are in sufficient stock and can be delivered on the same day as the order; 3. Desheng oil-based silicifying agent has the characteristics of repeated use. It can be used all the time without being used up and without pollution, saving cost.