FOR RESEARCH USE ONLY. Not for clinical diagnosis use
For the quantitative determination of Human MSH concentrations.
Reactivity: Human
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 0.3ng/ml-12 ng/ml
Sensitivity: 0.05ng/ml
Components:
Assay plate (12 8 coated Microwells)1
Standard:13.5ng/ml10.5ml
Standard Diluent11.5ml
HRP-Conjugate Reagent16ml
Sample Diluent16ml
Chromogen Solution A16ml
Chromogen Solution B16ml
Stop Solution16ml
Wash Solution120ml30 fold
User manual1
Adhesive Strip2
Product Principle:
The kit is for the quantitative level of MSH in the sample, adopt purified Human MSH antibody to coat microtiter plate,
make solid-phase antibody, then add MSH to wells, Combine MSH antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of MSH in the samples is then determined by comparing the O.D. of the samples
to the standard curve.
Details: Overview Reactivity: Human Methode Type: Sandwich ELISA Detection Quantity: 96 tests Sample type serum, plasma, Urine,tissue homogenates, cell culture supernates Detection range : 100ng/L-2000 ng/L Kit Components: Assay plate (12 8 coated Microwells) ..1 Standard: 2700ng/L 10.5ml Standard Diluent 11.5ml HRP-Conjugate Reagent 16ml Sample Diluent 16ml Chromogen Solution A16ml Chromogen Solution B16ml Stop Solution 16ml Wash Solution120ml30 fold User manual1 Adhesive Strip2 Product Principle: The kit is for the quantitative level of VC in the sample, adopt purified Human VC antibody to coat microtiter plate, make solid-phase antibody, then add VC to wells, Combine VC antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of VC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Introduction
Streptomycin (SM) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Streptomycin in honey, tissue, and etc.
Principle of test
The Streptomycin (SM) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The Streptomycin (SM) coupling antigen has been coated in the plate wells. During the detection, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a Horseradish peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. Take coloration with TMB substrate. The samples A value is an inverse relationship with SM residue content. Lastly, compared with the standard curve can be concluded the SM residues in sample.
The Salbutamal (Sal) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of clenbuterol in feed, tissue, and urine.
The method of Salbutamal (Sal) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The coupling antigen of Salbutamal has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target antigen. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the coated antigen attached to the well. Then add secondary antibody, tagged with a Horseradish Peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells.
The resulting color intensity, after addition of substrates, has an inverse relationship with the Sal residues concentration in the sample.
Introduction
The test kit is detecting for the quantitative analysis of Total Aflatoxin in cereal, feed, peanuts.
The Total Aflatoxin mainly infect corn, peanuts, nuts and etc.
principle of test
The Total Aflatoxin (AFT) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the
well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the toxin coated on the plate wells, last, take caloration with TMB substrate. The samples absorbance value is an inverse relationship with AFT content. Lastly, compared with the standard curve can be concluded the AFT residues in sample.
Technique Data
Kit sensitivity: 0.02ppb (ng/ml)
reactive mode: 25 , 30min 15min
Detection Limits:
Sample Detection Limits
Cereal 0.1 ppb
Compound feed 0.2ppb
Sauces, Wheat, Other feed 0.2ppb
Edible oil, peanut 0.2ppb
Beer 0.2 ppb
Wine, soy, vinegar 0.1 ppb
Cross-reaction rate:
Aflatoxin B1 . .100%
Aflatoxin B2 .. .75%
Aflatoxin G1 .. .100%
Aflatoxin G2 .97%
Aflatoxin M1 .. .30%
Sample Recovery rate:
Cereal, Compound feed .. . 85 15%
Peanut .. 82 15%
Edible oil .. 85 15%
Sauces, Wheat, Other feed .. 83 15%
Beer .. 84 15%
Wine, soy, vinegar .. 87 15%
