Details: Overview Reactivity: Human Methode Type: Sandwich ELISA Detection Quantity: 96 tests Sample type serum, plasma, Urine,tissue homogenates, cell culture supernates Detection range : 100ng/L-2000 ng/L Kit Components: Assay plate (12 8 coated Microwells) ..1 Standard: 2700ng/L 10.5ml Standard Diluent 11.5ml HRP-Conjugate Reagent 16ml Sample Diluent 16ml Chromogen Solution A16ml Chromogen Solution B16ml Stop Solution 16ml Wash Solution120ml30 fold User manual1 Adhesive Strip2 Product Principle: The kit is for the quantitative level of VC in the sample, adopt purified Human VC antibody to coat microtiter plate, make solid-phase antibody, then add VC to wells, Combine VC antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of VC in the samples is then determined by comparing the O.D. of the samples to the standard curve.