FOR RESEARCH USE ONLY. Not for clinical diagnosis use For the quantitative determination of Human MSH concentrations. Reactivity: Human Methode Type: Sandwich ELISA Detection Quantity: 96 tests Sample type serum, plasma, Urine,tissue homogenates, cell culture supernates Detection range : 0.3ng/ml-12 ng/ml Sensitivity: 0.05ng/ml Components: Assay plate (12 8 coated Microwells)1 Standard:13.5ng/ml10.5ml Standard Diluent11.5ml HRP-Conjugate Reagent16ml Sample Diluent16ml Chromogen Solution A16ml Chromogen Solution B16ml Stop Solution16ml Wash Solution120ml30 fold User manual1 Adhesive Strip2 Product Principle: The kit is for the quantitative level of MSH in the sample, adopt purified Human MSH antibody to coat microtiter plate, make solid-phase antibody, then add MSH to wells, Combine MSH antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of MSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.