FOR RESEARCH USE ONLY. Not for clinical diagnosis use
For the quantitative determination of Human MSH concentrations.
Reactivity: Human
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 0.3ng/ml-12 ng/ml
Sensitivity: 0.05ng/ml
Components:
Assay plate (12 8 coated Microwells)1
Standard:13.5ng/ml10.5ml
Standard Diluent11.5ml
HRP-Conjugate Reagent16ml
Sample Diluent16ml
Chromogen Solution A16ml
Chromogen Solution B16ml
Stop Solution16ml
Wash Solution120ml30 fold
User manual1
Adhesive Strip2
Product Principle:
The kit is for the quantitative level of MSH in the sample, adopt purified Human MSH antibody to coat microtiter plate,
make solid-phase antibody, then add MSH to wells, Combine MSH antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of MSH in the samples is then determined by comparing the O.D. of the samples
to the standard curve.
The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Androgen in samples. Add Androgen to monoclonal antibody Enzyme well which is pre-coated with Human Androgenmonoclonal antibody, incubation; then, add Androgenantibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concentration of the Human Substance Androgen of sample were positively correlated. Standard; 128nmol/L.
Cat/NO - O10014 Unit Size - 96T/48T Price - 39375/20475
Cat/NO - O10010 Unit Size - 96T/48T Price - 39375/20475
Cat/NO - O10016 Unit Size - 96T/48T Price - 39375/20475
Cat/NO - O10022 Unit Size - 96T/48T Price - 39375/20475
Cat/NO - O10025 Unit Size - 96T/48T Price - 39375/20475
Cat/NO - O10017 Unit Size - 96T/48T Price - 39375/20475
Cat/NO - O10018 Unit Size - 96T/48T Price - 39375/20475
Cat/NO - O10019 Unit Size - 96T/48T Price - 39375/20475
