Hepes

HEPES
CAS:7365-45-9

English Name:4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid
Molecular Formula:C8H18N2O4S
Molecular Wt:238.31
Appearance:Free flowing white powder
Assay(potertiometric cal on dry substance):99.8%.
Enterprise Standard.

Application: it is an important PH stable reagent in biotic experiments. Appropriate PH is obtained by mixing mild acid and its conjugate base. Many biological responses happen in neutral condition (usually PH 6-8), so the buffering range is required to be in PH 6-8. In addition, the form of acid-base of buffer should not chelate with some metallic ions.

Characteristics of biological buffer:
1, Strong water-solubility;
2. Compatibility of the acid-base dissociation balance;
3. Low membrance penetration rate;
4. Low metal chelating ability;
5. High chemical stability;
6. Low absorption in ultraviolet and visible region.



Packing:25KG / DRUM.

Introduction:
4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells.

Application:
HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile.

Buffer solution preparation:
(1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L.
(2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4�??�?�ºC or -20�??�?�ºC.

Purpose:
1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA
2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase
3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer

Introduction:
4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells.

Application:
HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile.

Buffer solution preparation:
(1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L.
(2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4�ºC or -20�ºC.

Purpose:
1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA
2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase
3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer

4-Hydroxyethylpiperazine Ethane Sulfonic Acid (HEPES) is a zwitterionic buffer, white crystalline powder, soluble in water, it is a weak acid, its pH buffer range is 6.8-8.2, it is important in biochemical industry Biochemical preparations are also considered to be one of the important buffers in the field of biological research.
As a veteran company with more than ten years of experience in producing HEPES, Desheng, the confidence we give to customers comes from our advantages in all aspects of products:
1. Advantages of PH range:
The suitable culture environment for most cells is pH 7.2-pH 7.4, and the application range of Desheng HEPES buffer just falls between pH 6.8-pH 8.2, which is in line with the characteristics of the pH value needed for cell culture.
Purity advantage:
Desheng HEPES has a purity of >=99%, good water solubility, stable process, and can control a constant pH range for a long time. According to your application needs, we can provide a full range of product testing and special testing services.
Reserve advantages:
Desheng has a professional R&D and production team, specializing in the production of HEPES, with a daily output of 1-2 tons, and advanced production technology and equipment. There will be no long supply cycle or supply outage. For customers, we enjoy the advantage of trial equipment.
Export advantages:
Desheng is a professional manufacturer of biological buffers, with strong logistics capabilities and rich export experience. We export to more than 100 countries and have established a long-term cooperation intention.
HEPES packaging advantages:
Only after using a good product can you know whether it is a good product in your mind. Desheng is not a company that only manufactures products once. What we want is the trust and support of our customers. We recommend that you use our trial pack first when purchasing the product. Because we firmly believe that quality is the greatest sincerity to give back to customers.

In many biological experiments, buffers are needed to maintain an effective pH value, and buffers are critical to the success or failure of the experiment.
When choosing a buffer, first consider that the pH value in the experiment is consistent with the pH value range of the buffer, and consider whether the advantages and disadvantages of different buffers are suitable for your experiment. There are many types of biological buffers. In this article, we mainly discuss the preparation methods of several commonly used biological buffers.
Prepare TRIS base and TRIS-HCL buffer
Tris: To prepare 1 liter of 1M Tris buffer, first prepare to dissolve 121.14 grams of Tris powder in 750 mL of dH20, adjust to the desired pH value with concentrated hydrochloric acid, and fill to the final volume of 1L with dH2O.
Tris HCl: To prepare 1 liter of 1M Tris HCl buffer, first dissolve 157.60 g of Tris-HCl in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill with dH2O to a final volume of 1L.
Prepare HEPES buffer
To prepare 1 liter of 1M HEPES buffer solution, first dissolve 238.3 grams of HEPES powder in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, fill with dH2O to a final volume of 1L, and pass through a filter or autoclave The device is disinfected. Store the buffer at 4C.
Prepare PIPES buffer
To prepare 1 liter of 1M PIPES free acid buffer solution, first add 302.37 g of PIPES free acid to 600 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill to the final volume of 1L with dH2O.
Desheng is a professional manufacturer of biological buffers. At present, the company sells buffers such as TRIS, HEPES, PIPES, CAPS, MOPS, BICINE, etc., with sufficient supply, fast delivery, guaranteed quality, and a professional after-sales team. If you answer any questions, you can now apply for a free sample online for trial. Please feel free to contact us if you need it.

Every biological system has a well-known buffer system to maintain the acid-base balance within a specific pH range. Our lives depend on the function of the buffer system. A buffer system is a solution that resists changes in pH when acid or alkali is added. Different buffers have different ability and efficacy for skin replacement. The high-quality Good's Buffers produced by Desheng are usually used in cosmetics, such as toners, essences, lotions, eye creams and facial masks, etc., according to your application. Here are 4 biological buffers commonly used in cosmetics.
1) HEPES (4-hydroxyethylpiperazine ethanesulfonic acid)
CAS Number: 7365-45-9
Useful pH range: 6.8-8.2
The main purpose
1. Acidity regulator, or pH control agent.
2. An effective concentration of HEPES can provide an exfoliating effect.
3. Promote the transdermal absorption of various functional ingredients in cosmetics.
2) TRIS (Tris(hydroxymethylaminomethane))
CAS number: 77-86-1
Useful pH range: 7.2-9.0
use:
1. Non-ionic surfactants-amines
2. Acidity regulator or pH control agent.
3. Excellent cleaning ability and emulsifying ability.
3) BIS-TRIS (Trimethylol) Methylamino Propane)
CAS Number: 5625-37-6
Useful pH range: 6.1-7.5
The main purpose
1. Non-ionic surfactants-amines
2. Acidity regulator, or pH control agent.
3. Excellent decontamination, dispersion, emulsification and antistatic ability.
4) Glycylglycine buffer (G-132)
CAS Number: 7365-44-8
Useful pH range: 7.5-8.9
The main purpose:
1. Adjust the pH range of the solution
2. Condition the skin, reduce pores and prevent skin aging.
3. Excellent emulsification, dispersion, antistatic, wetting, penetration, and decontamination capabilities
Precautions
External factors, water and detergents may reduce the local buffering capacity, because the elution of buffer chemicals leads to increased pH and irritant contact dermatitis. Inflammatory diseases, including atopic dermatitis, psoriasis, and acne vulgaris, increase in pH and decrease buffering capacity. For the treatment of skin diseases and aging skin, emollients with a pH value slightly higher than the average normal pH value and with appropriate buffering capacity should be used first.
Desheng is a professional manufacturer of biological buffers. There are many cooperative manufacturers in the skin care industry. In addition to the two biological buffers Hepes and Tris, it also produces many other models such as Mops, Taps, Pipes, Caps, and the core product tris buffer. Known for its ability to maintain the original color of emulsions and excellent odor suppression capabilities, it is widely used in cosmetics such as skin care lotions, sunscreens, eye makeup products, nail polish, etc. It can maintain the functional effectiveness of active substances and is a skin care product The industry's trusted choice.

De Sheng is a professional blood testing reagent company. It is a single enterprise with a complete industrial chain in China. Up to now, it has more than 10 years of R&D and production experience. Biobuffer products developed by Liu technical team of the company include Tris, Bicine, Caps, Mops, Taps, EPPS, HEPES, MOPSO, PIPES and so on, which are well received.
DeSheng brand Tri (hydroxymethyl) Angelica Tris introduced:
Trometamol
Name Trimethylol Angelica End
Tris
CAS# 77-86-1
Molecular Weight]121.135
Molecular Formula Adidine H11NO3
Storage Conditions Room temperature, light-proof and moisture-proof
Appearance White Crystal Powder
PH Scope] 7-9
Melting Point 168-172 degrees C
1. Dissolve Tris with a small amount of double-steamed water (300-500 ml). After adding HCl, adjust the pH to 7.6 with HCl (1N) or NaOH (1N), and add extra-thick double-steamed water to 1000 ml. This liquid is a reserve liquid, which is stored in refrigerator at 4 C.
Uses: Buffer preparation in biochemical and molecular biology experiments; intermediate; preparation of surfactants and vulcanization accelerators; preparation of water-soluble polymers containing Tris structural units as paint dispersants; preparation of indoor formaldehyde adsorption materials, etc., Tris is also used as titration standard.
1. Buffering properties] Tris is a weak base, and its pKa is 8.1 at room temperature (25 C); according to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2.
Desheng products include good's buffer: Bicine, Caps, EPPS, MOPS, TAPS, HEPES, MOPSO, PIPES; blood vessel extracting additives: serum separating gel, sodium glycerin, glycerin, EDTA dipotassium, EDTA tripotassium, coagulant, planning agent, sodium citrate, planning potassium; chromogenic substrates: TOOS, TOPS, ADOS, ADP S, ALPS, DAOS, HDAOS, MADB, MAOS, PEP, etc. Luminescent reagents: Azidine ester, Luminol, Azidine salt and isoluminol. All in vitro diagnostic reagents are purified by HPLC analysis method and the purity is more than 99%. As the manufacturer develops and sells, the DeSheng color substrates are in stock and the quality is guaranteed. Enterprise friends who need color raw materials can contact Desheng, address C8, Guanggu United Science and Technology City, Ezhou, Hubei Province, and look forward to your visit!