Desheng Product Tris

The Chinese name of Tris is tris(hydroxymethyl)aminomethane, tromethamine, tromethamine, 2-amino-2-(hydroxymethyl)-1,3-propanediol. It is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in ether and carbon tetrachloride, corrosive to copper and aluminum, and irritating chemicals. Tris has a high buffer capacity, high solubility in water, and is inert to many enzymatic reactions, making Tris a very satisfactory buffer for many biochemical purposes. It is generally used to stabilize the pH of the reaction system, and has a strong buffering capacity between pH 7.5-9.0. Advantages of Tris buffer: 1. Because Tris base is more alkaline, you can only use this buffer system to configure a buffer with a wide range of pH ranging from acidic to alkaline; 2. It has little interference to biochemical processes, and does not precipitate with calcium, magnesium ions and heavy metal ions. Disadvantages of Tris buffer: 1. The pH of the buffer is greatly affected by the concentration of the solution, the buffer is diluted ten times, and the pH change is greater than 0.1; 2. The temperature effect is large, and the temperature change has a great influence on the pH of the buffer. The pH of the buffer at 4�?�°C is 8.4, and the pH at 37�?�°C is 7.4. It must be prepared at the temperature of use, and prepared at room temperature Tris-HCL buffer cannot be used for 0-4; 3. It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed; 4. This buffer has a certain interference effect on some PH electrodes, so use electrodes that are compatible with Tris solution. Application of Tris buffer: It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH; uses the Tris-HCL buffer system in the gel to stabilize the pH; it is widely used as a solvent for nucleic acids and proteins; the low ionic strength of the Tris buffer can also be used The formation of intermediate fibers of nematodes; add EDTA to Tris hydrochloric acid buffer to make "TE buffer", which can be used for DNA stabilization and storage; change the pH-adjusting acid solution to acetic acid to obtain "TAE buffer", change "TBE buffer" can be obtained as boric acid. These two solutions are used in nucleic acid electrophoresis experiments.

Hubei Xindesheng was established in 2017. It is a new technology-based enterprise established on the basis of Wuhan Desheng Biochemical Technology Co., Ltd. (2005). After years of innovation and development, the company has now become a leader in "blood collection tube additives", "chemiluminescence reagents", "biological buffers", "color reagents", etc., integrating R&D, production, sales and service. high-tech enterprises. Tris, as the company's main advantage product in biological buffers, has always had its own advantages: 1. TRIS is used as a neutralizer in cosmetics, which is actually a PH regulator. 2. Tris is used as a solvent for nucleic acids and proteins and as a biological buffer. 3. It can be used to prevent and treat metabolic acidosis and respiratory acidosis; 4. Tris is used to prepare surfactants, vulcanization accelerators and intermediates for some drugs. 5. Tris mainly acts as a pH regulator, cross-linking accelerator, polyester coating raw material and phase change core material in the coating preparation process; 6. Products related to Tris can be prepared through the molecular structure of Tris. Such as Bis-Tris, Tricine, TES, TAPS, etc. From the above points, we can see that Tris itself has a wide range of applications, but it is not a simple matter to make high-quality Tris. In the past ten years, Desheng has been learning and improving its technology. The Tris produced is white crystalline powder; it has good solubility in water systems and minimal solubility in organic solvents; it is in dry or solution state. It has excellent stability and can resist enzymatic or non-enzymatic degradation; it has a very small absorption value for visible light and ultraviolet light. Therefore, when we store tris, we only need to store it in a cool, dry and moisture-proof warehouse at room temperature. Desheng is a professional TRIS manufacturer, committed to providing customers with high-quality products and services that can meet the special requirements of R&D customers for product types, packaging, purity and other aspects.

In many biological experiments, buffers are needed to maintain an effective pH value, and buffers are critical to the success or failure of the experiment. When choosing a buffer, first consider that the pH value in the experiment is consistent with the pH value range of the buffer, and consider whether the advantages and disadvantages of different buffers are suitable for your experiment. There are many types of biological buffers. In this article, we mainly discuss the preparation methods of several commonly used biological buffers. Prepare TRIS base and TRIS-HCL buffer Tris: To prepare 1 liter of 1M Tris buffer, first prepare to dissolve 121.14 grams of Tris powder in 750 mL of dH20, adjust to the desired pH value with concentrated hydrochloric acid, and fill to the final volume of 1L with dH2O. Tris HCl: To prepare 1 liter of 1M Tris HCl buffer, first dissolve 157.60 g of Tris-HCl in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill with dH2O to a final volume of 1L. Prepare HEPES buffer To prepare 1 liter of 1M HEPES buffer solution, first dissolve 238.3 grams of HEPES powder in 750 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, fill with dH2O to a final volume of 1L, and pass through a filter or autoclave The device is disinfected. Store the buffer at 4C. Prepare PIPES buffer To prepare 1 liter of 1M PIPES free acid buffer solution, first add 302.37 g of PIPES free acid to 600 mL of dH2O, adjust to the desired pH with 10N sodium hydroxide, and fill to the final volume of 1L with dH2O. Desheng is a professional manufacturer of biological buffers. At present, the company sells buffers such as TRIS, HEPES, PIPES, CAPS, MOPS, BICINE, etc., with sufficient supply, fast delivery, guaranteed quality, and a professional after-sales team. If you answer any questions, you can now apply for a free sample online for trial. Please feel free to contact us if you need it.

Every biological system has a well-known buffer system to maintain the acid-base balance within a specific pH range. Our lives depend on the function of the buffer system. A buffer system is a solution that resists changes in pH when acid or alkali is added. Different buffers have different ability and efficacy for skin replacement. The high-quality Good's Buffers produced by Desheng are usually used in cosmetics, such as toners, essences, lotions, eye creams and facial masks, etc., according to your application. Here are 4 biological buffers commonly used in cosmetics. 1) HEPES (4-hydroxyethylpiperazine ethanesulfonic acid) CAS Number: 7365-45-9 Useful pH range: 6.8-8.2 The main purpose 1. Acidity regulator, or pH control agent. 2. An effective concentration of HEPES can provide an exfoliating effect. 3. Promote the transdermal absorption of various functional ingredients in cosmetics. 2) TRIS (Tris(hydroxymethylaminomethane)) CAS number: 77-86-1 Useful pH range: 7.2-9.0 use: 1. Non-ionic surfactants-amines 2. Acidity regulator or pH control agent. 3. Excellent cleaning ability and emulsifying ability. 3) BIS-TRIS (Trimethylol) Methylamino Propane) CAS Number: 5625-37-6 Useful pH range: 6.1-7.5 The main purpose 1. Non-ionic surfactants-amines 2. Acidity regulator, or pH control agent. 3. Excellent decontamination, dispersion, emulsification and antistatic ability. 4) Glycylglycine buffer (G-132) CAS Number: 7365-44-8 Useful pH range: 7.5-8.9 The main purpose: 1. Adjust the pH range of the solution 2. Condition the skin, reduce pores and prevent skin aging. 3. Excellent emulsification, dispersion, antistatic, wetting, penetration, and decontamination capabilities Precautions External factors, water and detergents may reduce the local buffering capacity, because the elution of buffer chemicals leads to increased pH and irritant contact dermatitis. Inflammatory diseases, including atopic dermatitis, psoriasis, and acne vulgaris, increase in pH and decrease buffering capacity. For the treatment of skin diseases and aging skin, emollients with a pH value slightly higher than the average normal pH value and with appropriate buffering capacity should be used first. Desheng is a professional manufacturer of biological buffers. There are many cooperative manufacturers in the skin care industry. In addition to the two biological buffers Hepes and Tris, it also produces many other models such as Mops, Taps, Pipes, Caps, and the core product tris buffer. Known for its ability to maintain the original color of emulsions and excellent odor suppression capabilities, it is widely used in cosmetics such as skin care lotions, sunscreens, eye makeup products, nail polish, etc. It can maintain the functional effectiveness of active substances and is a skin care product The industry's trusted choice.

|English name|3-[Tris(hydroxymethyl)methylamino]-1-propanesulfonic acid |Chinese name|N-tris (hydroxymethyl) methyl-3-aminopropylsulfonic acid |Abbreviation|TAPS |CAS#|29915-38-6 |Molecular weight|243.28 |Molecular formula|C7H17NO6S |Storage condition|room temperature, away from light and moisture |Molecular structure| |Application|This product can be used as a buffer system commonly used in DNA screening system and as a buffer component of RNA sample. It is suitable for the study of electron transfer and phosphorylation of chloroplast thin layer preparation. It can protect oxyhemoglobin from oxidation to methemoglobin in the process of freeze-drying. It can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis. |Product advantage|The purity is >99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder. Desheng is a chemical reagent company specializing in blood test reagents with 15 years of R&D experience, especially has a deep research on chromogenic substrate and biological buffer. Enterprises or individuals in need can directly contact

The structure and function of the protein need to be maintained in a suitable pH environment. Therefore, the choice of pH buffer is very important during the preparation and purification of the protein. Only a suitable buffer can ensure the activity and structural stability of the protein. Effect of buffer in protein purification process There are many kinds of buffers for protein purification. While maintaining the pH of the system, it is necessary to ensure that the protein cannot be inactivated during the purification process, or to minimize the amount of protein inactivation, which must be maintained in every step of protein purification. Solubility and activity of protein. Protein purification buffer requirements The buffer in the protein purification process should not only prevent protein degradation, but also prevent protein aggregation, which has a significant impact on the experimental results. The following factors should be considered when selecting and reagent buffers: pH, buffer system, salt ions, reducing agents and stabilizers. Each of these factors should be optimized according to the purified target protein, and the activity of the target protein should be used as an evaluation criterion for optimization. Understand protein stability conditions There are many types of proteins, and different proteins adapt to different pH values. The buffer should be selected according to its optimal pH value. In many cases, the pH is 7.4, which is a mimic biological condition, and most proteins are stable at this pH. However, there are some exceptions, which require pH adjustment to keep the protein soluble and not degraded. Commonly used buffers for protein purification Commonly used buffers in the protein purification process include Tris, Tricine, Bis-Tris, MOPS, etc. Among them, Tris is commonly used. Its buffer range is close to physiological pH and has a low ionic strength. It is a good solvent for proteins. Can ensure the stability of the protein. It should be noted that when preparing the buffer, try to avoid the isoelectric point pl of the target protein. Because the protein has no electrostatic charge on its surface in the pH solution of its isoelectric point, the repulsive force between the hundred molecules is reduced, it is easy to aggregate, and the solubility is reduced. Now the adaptation pH value, isoelectric point, and buffer range of the buffer to be purified can be found, and you can directly match it according to the corresponding requirements. Desheng is a manufacturer of in vitro diagnostic reagents, which can provide large-package pH buffers such as Tris, MOPS, Bicine, etc.