Proteinase K CAS NO 39450-01-6 Lyophilized Powder from Tritirachium album limber. CAS No. 39450-01-6 Molecular Formula: C29H27N2O12P Molecular Weight: 626.504641 EINECS NO. :254-457-8 Other name: CHLOROM; IUB:3.4.21.14; IUB: 3.4.21.64; EC 3.4.21.14; EC 3.4.21.64; ENDOPEPTIDASE K; 3.4.21.14; PROTEINASE K. Protease k is a highly active protease of the subtilisin class, used for the general degradation of proteins in biological samples. It is purified from Tritirachium album limber. Protease K is a serine protease with broad cleavage activity. It cleaves the carboxy terminal peptide bond of aliphatic amino acid and aromatic amino acid. This enzyme is purified to remove RNase and DNase activities. Since proteinase K is stable in urea and SDS, it also has the ability to degrade natural proteins. Application: 1.Gene Diagnostic Kit 2.Genomic DNA extraction kit 3.RNA extraction kit removes nucleases from DNA and RNA preparation 4.Extract non-protein components from tissues to degrade protein-containing impurities-such as preparation of DNA vaccines and heparin 5.Preparation of chromosomal DNA for pulse electrophoresis 6.Western Blot 7.The general working concentration of proteinase K is 50-100ug / ml Proteinase K Source: Tritirachium album limber Appearance: white lyophilized powder Specific activity: > 30 U / mg protein (measured 33-36U / mg, subject to the current batch of COA). Molecular weight: 29kDa (SDS-PAGE) Definition of activity unit: at 37 and pH 7.5, the substrate casein can be hydrolyzed to produce 1 �¼mol of tyrosine eggs per minute The amount of white enzyme K is defined as one unit (U). Residue of DNase: Digested with �» DNA as the substrate for more than 6 hours at 37 , no deoxyribonuclease activity was detected. RNase residue: Digested at 25 for more than 16 hours, no ribonuclease activity was detected. Inactivation method: adding benzenesulfonyl chloride (PMSF) or diisopropyl fluorophosphoric acid (DFIP) inhibitor to the reaction system Alive, incubation at 65 for 10-15min can partially inactivate. Packing: 100g / bottle; 500g / bottle; 1Kg / bottle Storage: The dry powder state can be stored for a long time under the condition of -20 . After dissolving, dispense into appropriate volume, store at 2-8 for short-term, and -20 for long-term. Under the correct storage conditions, the validity period of the dry powder state can reach one year. The liquid state is valid at -20 for half a year. After opening the package, if It is recommended to filter and sterilize or add a stabilizer such as 0.5% sodium azide to prevent microbial contamination if it is left at 2-8 for more than a week. Storage and dilution buffer: 50 mM Tris-HCL buffer, 2 mM CaCL2 (pH 8.0). Reaction conditions: Incubate for one hour at 37-56 in reaction buffer
Cationic surface sizing agent ( compete with bk532) The main data: Product name: cationic surface sizing agent (sae based) Appearance: milk white to light brown liquid The main description: Cationic surface sizing agent is a new type of surface sizing agent that synthesized by the copolymerization of styrene and ester. It can efficiently combinate with starch and lead to good cross link intensity and hydrophobic properties.With lower dosage, lower cost and easy use advantages, it has good film-forming property which can significantly improve the water-resistance and ring-crush strength of board paper and corrugated paper.No curing period.Completely replace the usage of internal sizing agent. The main application: Its correspondence product name is bk532.Based on large quantity application experience in varies paper mills, we improve the recipe day by day, finally quality is the top grade in the market.It can be used with starch to reduce sizing cost.It also has good mechanical stability with less bubbles generated during the operational process.After finish the starch cooking, keep the temperature at about 70-75, add in this product based on 1-2kg per ton paper(actual dosage should be depend on practice), use alum to adjust the ph to be 3.5-4.0, then metering in size press. Packing & storage: Packing: 200kgs pp drum or 1000kgs ibc drum Storage: store in a cool, dry place. When loading and unloading, you should take care to prevent packaging and containers being damaged, avoiding leakage. Quality standard: Item Standard specification Appearance Milk white to light brown liquid Ionic Cationic Solid content 30%min Ph 5max Viscosity(25).Cps 30max Shelf life 6-12months
Anionic surface sizing agent (styrene acrylic based) The main data: Product name: anionic surface sizing agent (sae based) Appearance: white to light brown liquid The main description: Anionic surface sizing agent is a new type of surface sizing agent that synthesized by the copolymerization of styrene and ester.It can efficiently combinate with starch result good cross link intensity and hydrophobic properties.With lower dosage, lower cost and easy use advantages, it has good film-forming property which can significantly improve the water-resistance and ring-crush strength of culture paper such as offset printing paper and electro-photographic paper.Our quality can compete with basf 400ds and basf8903/8906. The main application: It can be applied in combination with anionic and nonionic auxiliaries such as starch, direct dyes, acid dyes and fluorescent whitening agents, etc.The paper does not necess arily have to be sized at the wet end and before it is applied, but this can be an effectiv e means of controlling the extent to which the size is able to penetrate. No curing period, completely replace the usage of internal sizing agent.It can be used with starch to reduce sizing cost.It also has good mechanical stability with less bubbles generated during the operational process.Application temperature should be less than 75 Packing & storage: Packing: 200kgs pp drum or 1000kgs ibc drum Storage: store in a cool, dry place. When loading and unloading, you should take care to prevent packaging and containers being damaged, avoiding leakage. Quality standard: Item Standard specification Appearance White to light brown liquid Ionic Anionic Solid content 25%min Ph 2-4 Viscosity(25).Cps 50max Shelf life 6-12months
Paper sizing agent - alkyl ketene dimer 1840 Product description: 1. Alkyl ketene dimer 1840 is a kind of fiber reactive neutral sizing agent, with high content of effective component, high iodine value and low acidity. It is an efficient sizing agent, especially suitable for the environmental protection requirements. 2. The related items ItemsAkd wax 1840 AppearancePale yellow wax solid Purity91% Iodine calue(gi2/100g )45.0 min Acid value(mgkoh/g)3.0 max Melting point(c)47.0 min Application: As a neutral sizing agent, alkyl ketene dimer is widely used in the papermaking industry. Alkyl ketene dimer must be made into akd emulsion to used in paper sizing. It can make paper less water absorbing and controls its printing properties. Packing and delivery: Packing: packed in 25kg pp/pe bag, 500kg jumbo bag, or others according to clients Delivery: within 7 days after order confirm Storage: store in dry and ventilated place with temperature not exceeding 35 c The storage period is twelves month Product introduction: According to c18 content, it has akd wax 1840, akd wax 1865, akd wax 1895.Based on our own phosgene project, using top grade stearic acid, auto production under dcs control, all these make sure our final akd wax quality is the top grade, purity can be 91%min.We not only consider on supplying akd wax, but also consider more on technology support and recipe improvement sharing. Welcome to visit http://www.Glchemicals.Net for more related products information. Akd wax 91% with high quality made in chinaakd wax 91% with high quality made in china
Leather Goods : Bangladeshi Leather goods are largely accepted all over the world for it quality and unique styles. Bangladeshi leather goods manufacturers are well experienced. The government of Bangladesh has paying special interest of enlargement of Leather Industry. Due to our food habit and religious tradition Bangladeshi leather goods manufacturers find a huge number of raw Cow, Ships, Camel and Goat leather every year. So our leather quality is exceptional from other countries. Due to cheap labour market and availability of leathers, our manufacturers can produce leather products much cheaper than any other countries. This is why Bangladesh leather products are widely accepted and we are exporting huge number of leather goods every year. Leather goods are one of the largest parts of our economy. We have reliable and well know Bangladeshi manufacturers who manufacture different goods in their own industry by well experienced engineers and workers. ‘Expo BD’ has aim to export quality goods to foreign countries and also represent Bangladeshi goods to the world market. We ensure the product quality for our valuable buyers. We only confirm the order if we believe that, we can ensure the right product with the best quality. ‘Expo BD’ ensures the delivery of the product within the time frame. We process minimum exportable order at any destination. Here are some pictures of our products bellow. Anyone can request any of these products and products can be delivered on time in any countries.If any buyer wants to order goods of his own design with Logo/monogram/Name, that can be done as well. We want to confirm that our manufacturers can get the customized products done as per the requirements of the clients. Our Leather Items: Professional Office Bag (Men & Women) Purse Wallet Jacket (Men & Women) Hand Gloves (Men & Women) Travel Bags BackPacks Notebook Covers Belts (Men & Women) Card Holders
- Any Jute bags/sacks - According Buyers requirement
Coriander Seeds : Coriander (dhania) is a strong-smelling annual herb, Coriandrum sativum. It grows in Bangladesh very well at winter time and fulfills the demand of the country. Bangladeshi farmers harvest a huge amount of coriander in their field during the winter and after fulfilling the demand of the country, Bangladesh exports a lot of coriander seeds in different countries. Coriander has many health benefits of controlling Blood Sugar, Cholesterol & Blood Pressure. Coriander seeds and its leaves are also being used as spice in different foods for flavor and taste.
Specifications: - Color: Yellow/Red - Purity: 95% - Moisture: 12% - Ash: 6% max. - Acid insoluble ash 1% - Volatile oil: 02% max
Specification : Purity: 99% Min Other Color 1 % Moisture: 5-7% Acidity: 3 % Admixture: 1 % Oil Contain: 48 % Color: Dark Black
Specifications: - Fat/Oil -20% - Moisture â?? 12% max - Fat/Oil -20% - Moisture â?? 12% max - Fiber â?? 11% max
Specification: - Moisture - 10.30% - Crude Protein - 16.92% - Fiber - 11.60% - Oil Content - 0.34% - Sand Silica - 2.80%
According buyers requirement
According buyers requirements.
- Leather products - Ladies bag - Ladies purse - Office bag - Laptop bag - Note book cover - Card holder - Leather Gloves - Leather jackets - Finished leather - Leather travel bag - Leather backpack - Leather Key Ring
Specification: - with handle - without handle - any color - woven bags - non woven bags - shopping bags
Specifications: - Color: Yellow White, Black, Brown and Small White (small white only available during November & December) - Purity: 100% - Moisture: 5% - Oil Content: 45% - FFA: 1.5% - Protein: 18% - Certificates are available upon request.
As per buyers requirement
- Wall hangings - Chairs - Sofa - Photo Frame - Mirror Frame etc. - Material: Rattan, Bamboo & Wood Can be manufactured as per buyer's requirement.
Canyearn C85 Full Digital Trolley Ultrasonic Diagnostic System Color Doppler Ultrasound Scanner with touch screen General Specification 1. Main Features � Operationg SyetemPC Windows platform Monitor 15.6 inch LED high-resolution monitor with rotatable arm and a touch screen Scanning ModeB, M, C, D, CW Dynamic Range0-150dB Cine LoopUp to 1024 frames Gain0-100dB TGC8 segment Scanning DepthUp to 360mm Magnification?1.5 - ?5.0 Boby Marking17 Probe Connector4 LanguageChinese/English Matching PrinterLaser/Vedio/Inkjet/Thermal printer Peripheral PortUSB, VGA, DICOM, RS232 Blood Flow ImageB, CW, C(=CFM), D(=PW) Normal MeasurementDistance, Area, Angle, Volume, Trace, Diam red(diameter stenosis), Area red(area stenosis), Cross curve, Parallel lines M General: ET, HR, Vel, Dis D General: ET, HR, Vel, ACC, Common, Trace Professional MeasurementAbdominal, Cardiology, Urology, Vascular, Early Obstetric, Later Obstetric, General, MSK, Pelvis Small organs(right/left thyroid) Combination of Digital Ultrasonic TechnologyThe multi-core intel processor The wideband digital beam forming The multi beam parallel processing Trapezoid Imaging Accurate blood flow imaging Intelligence speckle suppression Wide view imaging Intelligence screen capture One touch optimization  2. Standard Configuration Main system unit, 3.5MHz Convex probe, 7.5 MHz Linear Probe, Image storage unit, Specialist report, Image processing optimization, User manual and disc 3. Optional Items 3D+4D, Biopsy Bracket, USB disk 4. Probes Selection 3.5 MHz Convex Probe (3.5-14MHz variable frequency ) 7.5 MHz Linear Probe (3.5-14MHz variable frequency ) 7.5 MHz Transvaginal Probe (3-10MHz variable frequency) 5.0 MHz Micro-Convex Probe (2-6MHz variable frequency) 7.5 MHz Rectal Probe(80 elements) 3D/4D Probe Cardiac Probe 6. Advanced Technologies PC platform One Touch Optimizer Tissue Harmonic Imaging 3D/4D Imaging Reconstruction DICOM 3.0 Packaging & Delivery Packaging Details: Wooden Box Package� Net Weight: 80 kg Gross Weight: 89 kg Package Size: 1044*644*1245mm Delivery Time: 5-10 working days
Canyearn C75 Full Digital Trolley Ultrasonic Diagnostic System Color Doppler Ultrasound Scanner General Specification 1. Main Features � Operationg SyetemPC Windows platform Monitor 15 inch LED high-resolution monitor with rotatable arm Scanning ModeB, 2B, B/M, 3D/4D Dynamic Range0-150dB Cine LoopUp to 1024 frames Gain0-100dB TGC8 segment Scanning DepthUp to 300mm Magnification2.0 - 10 Body Marking63 Probe Connector4 LanguageChinese/English Matching PrinterLaser/Vedio/Inkjet/Thermal printer Peripheral PortUSB, LAN, S-VIDEO, RS232 Blood Flow ImageCW, CFM, PW Normal MeasurementDistance, Area, Angle, Volume, Trace, Diam red(diameter stenosis), Area red(area stenosis), Cross curve, Parallel lines M General: ET, HR, Vel, Dis D General: ET, HR, Vel, ACC, Common, Trace Professional MeasurementAbdominal, Cardiology, Urology, Vascular, Early Obstetric, Later Obstetric, General, MSK, Pelvis Small organs(right/left thyroid) Combination of Digital Ultrasonic TechnologyThe multi-core intel processor The wideband digital beam forming The multi beam parallel processing Trapezoid Imaging Accurate blood flow imaging Intelligence speckle suppression Wide view imaging Intelligence screen capture One touch optimization � 2. Standard Configuration Main system unit, 3.5MHz Convex probe, 7.5 MHz Linear Probe, Image storage unit, Specialist report, Image processing optimization, User manual and disc 3. Optional Items 3D+4D, Biopsy Bracket, USB disk 4. Probes Selection 3.5 MHz Convex Probe (3.5-14MHz variable frequency ) 7.5 MHz Linear Probe (3.5-14MHz variable frequency ) 7.5 MHz Transvaginal Probe (3-10MHz variable frequency) 5.0 MHz Micro-Convex Probe (2-6MHz variable frequency) 7.5 MHz Rectal Probe(80 elements) 3D/4D Probe Cardiac Probe 6. Advanced Technologies PC platform One Touch Optimizer Tissue Harmonic Imaging 3D/4D Imaging Reconstruction DICOM 3.0 Packaging & Delivery Packaging Details: Wooden Box Package� Net Weight: 70kg� Gross Weight: 79kg Package Size: 700*790*930mm Delivery Time: 5-10 working days Warranty Two years for main unit (One year warranty for probes)