Proteinase K CAS NO 39450-01-6 Lyophilized Powder from Tritirachium album limber. CAS No. 39450-01-6 Molecular Formula: C29H27N2O12P Molecular Weight: 626.504641 EINECS NO. :254-457-8 Other name: CHLOROM; IUB:3.4.21.14; IUB: 3.4.21.64; EC 3.4.21.14; EC 3.4.21.64; ENDOPEPTIDASE K; 3.4.21.14; PROTEINASE K. Protease k is a highly active protease of the subtilisin class, used for the general degradation of proteins in biological samples. It is purified from Tritirachium album limber. Protease K is a serine protease with broad cleavage activity. It cleaves the carboxy terminal peptide bond of aliphatic amino acid and aromatic amino acid. This enzyme is purified to remove RNase and DNase activities. Since proteinase K is stable in urea and SDS, it also has the ability to degrade natural proteins. Application: 1.Gene Diagnostic Kit 2.Genomic DNA extraction kit 3.RNA extraction kit removes nucleases from DNA and RNA preparation 4.Extract non-protein components from tissues to degrade protein-containing impurities-such as preparation of DNA vaccines and heparin 5.Preparation of chromosomal DNA for pulse electrophoresis 6.Western Blot 7.The general working concentration of proteinase K is 50-100ug / ml Proteinase K Source: Tritirachium album limber Appearance: white lyophilized powder Specific activity: > 30 U / mg protein (measured 33-36U / mg, subject to the current batch of COA). Molecular weight: 29kDa (SDS-PAGE) Definition of activity unit: at 37 and pH 7.5, the substrate casein can be hydrolyzed to produce 1 �¼mol of tyrosine eggs per minute The amount of white enzyme K is defined as one unit (U). Residue of DNase: Digested with �» DNA as the substrate for more than 6 hours at 37 , no deoxyribonuclease activity was detected. RNase residue: Digested at 25 for more than 16 hours, no ribonuclease activity was detected. Inactivation method: adding benzenesulfonyl chloride (PMSF) or diisopropyl fluorophosphoric acid (DFIP) inhibitor to the reaction system Alive, incubation at 65 for 10-15min can partially inactivate. Packing: 100g / bottle; 500g / bottle; 1Kg / bottle Storage: The dry powder state can be stored for a long time under the condition of -20 . After dissolving, dispense into appropriate volume, store at 2-8 for short-term, and -20 for long-term. Under the correct storage conditions, the validity period of the dry powder state can reach one year. The liquid state is valid at -20 for half a year. After opening the package, if It is recommended to filter and sterilize or add a stabilizer such as 0.5% sodium azide to prevent microbial contamination if it is left at 2-8 for more than a week. Storage and dilution buffer: 50 mM Tris-HCL buffer, 2 mM CaCL2 (pH 8.0). Reaction conditions: Incubate for one hour at 37-56 in reaction buffer
1,2,3-bezotriazol 1,2,4-tri Chloro Benzene 1,3,4-xylidine-5-sulphonic Acid 1,4-sulphophenyl-3-methyl-5-pyrazolone (Spmp) 2-aminophenol-4-sulphonic Acid(Oapsa) 2-chloro-4-nitro Aniline[ortho Chloro Para Nitro Aniline](Ocpna) 2-nitro-4-amino Diphenylene-4-sulphonic Acid(2-nadpsa) 2,4-di Nitro Aniline 2-4-di Nitro Chloro Benzene 2,4,5-tri Chloro Aniline 2,4,5-tri Chloro Nitro Benzene 2,5-dichloro Aniline(Scarlet Gg Base) 3-di Ethyl Amino Phenol (Demap) 3,3-di Chloro Benzedine Di Hydrochloride 3,3-di Chloro Benzene 3,4-di Chloro Aniline 3,4-di Chloro Nitro Benzene(3,4-dcnb) 4-chloro-2-amino Phenol[2-amino-4-chloro Phenol](4 Cap) 4-nitro-2-amino Phenol(4 Nap) 4-nitro-4-amino Diphemylene-2-sulphonic Acid(4-nadpsa) 4-sulpho Formazone Base 4,4 Diamino Sulphanilide 4,4 Diamino Benzsulphanilide (Dasa) 4,4-di Amino Benzidine-2,2-di Sulphonic Acid 4,4-di Amino Diphenyl-2,2-di Sulphonic Acid 4,4-di Amino Stilbene, 2:2 Di Sulphonic Acid (Dasda) 5-nitro-2-amino Phenol(5 Nap) 6-acetyl Amino-2-amino Phenol-4-sulphonic Acid 6-chloro Metanilic Acid 6-nitro 1-diazo 2-naphthol 4-sulphonic Acid 6-nitro-2-amino Phenol(6 Nap) Alpha Naphthylamine Amino G-acid Aniline-2,4-di Sulphonic Acid Anthranilic Acid Benzanthrone Benzyl Benzoate Chloranil Di Anisidine Base Di Mehtyl Sulphate F.c.acid(4,4-diamino Di Phenyl Amine 2-sulphonic Acid) Gamma Acid G-salt(2-hydroxy Naphthalene 6-8 Disulphonic Acid) H-acid(1-amino-8-napthol-3-6-di Sulphonic Acid) K Acid(Schaeffer's Acid )(2-hydroxy Napthalene-6-sulphonic Acid) Meta Phenylene Di Amine Di Sulphonic Acid (Mpddsa) Meta Phenylene Di Amine-4-sulphonic Acid(Mpdsa) Meta Ureido Aniline(Mua) Metanilic Acid N-methyl J-acid N-phenyl J-acid Ortho Amino Phenol 4 Sulfonamide (O.a.p.s.a Amide) Ortho Anisidine Para Sulphonic Acid (Oapsa) Ortho Chloro Para Nitro Aniline(Ocpna) Ortho Di Chloro Benzene[1,2-di Chloro Benzene](Odcb) Ortho Dianisidine Base Ortho Nitro Chloro Benzene (Oncb) Ortho Nitro Chlorobenzene 4-sulphonic Acid(Oncbsa) Ortho Tertiary Butyl Cyclo Hexyl Acetate Ortho Toluidine Para Amino Acetanilide-3-sulphonic Acid(Paa 3sa) Para Amino Azo Benzene 4 - Sulfonic Acid (Paab 4sa) Para Amino Azobenzene-3-4-di Sulphonic Acid(Oncbsa) Para Amino Azobenzene-4-sulphonic Acid Para Anisidine Ortho Sulphonic Acid Para Anisidine-3-sulphonic Acid Para Chloro Ortho Nitro Aniline[4 Chloro 2 Nitro Aniline](Pcona) Para Di Chloro Benzene(Pdcb) Para Nitro Aniline 2-sulphonic Acid Para Nitro Aniline(Pna) Para Nitro Chloro Benzene Ortho Sulfonic Acid(Pncbosa) Para Nitro Chloro Benzene(Pncb) Para Phenylene Diamine Di Sulphonic Acid(Ppddsa) Phenyl Hydrazine Para Sulphonic Acid R Salt Resist Salt Sodium Naphthionate Sodium Sulphanilate Sulphamic Acid Sulphanilic Acid Sulpho Tobias Acid Tertiary Butyl Catechol[1,2 Dihydroxy-4,tertiary Butyl Benzene] Vinyl Sulphone Ester Acetanilide Base Vinyl Sulphone Ester Ortho Anisidine Base Vinyl Sulphone Ester Para Base Ester
Item Standard Result Purity, % 99.95 99.98 Water, Wt%. PPm% 50 35 Alkalinity, NaOH, % 0.03 0.01 Density P20/g/cm3 1.615-1.625 1.624 ColourHazen, 15 10 Non-volatilematter, % 0.005 0.002.
Item: Standard result Appearance colorless liquid confirm Moisture: 0.2%max 0.074 Acid: 0.01%max 0.001 Content: 99.5%min 99.8%.
Gum rosin is an import chemica and it mainly used in soap industry, paper industry, paint and coatings industry, ink industry, adhesives, rubber industry, food industrial, electric industry and so on.
1. White Flake and Briquette 2. Purity: 99.5% 3. Packing: 25kg/bag,22mt/FCL 4. Usage: Manufacture of unsaturate polyester resin, alkyd resin, insulate paint, plasticicer, maleic acid, malaic acid and tartaric acid, etc. It can be used antiseptic agent, also serve as the raw materials of medicine, paint, glass steel and so on. 5. Packing: 25kg/bag ,500KG/bag ,600kg/jumbo bag IN 20' FCL 20T/22T(with/without pallets).
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Services: Buying agents, importing, exporting
Buyer: Decoration, giftware, toys & games, garments like shirts, t shirts, polo, pant and trouser, underwear and sportswear, household like kitchenware, stationery & foodstuffs, mineral items, iron ore, metal scrap, petroleum items, chemical items, agricultural products like rice, edible oils like sunflower oil, animal feeds, paper products, wood pellets & wood chips
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Services: End to end trading
Buyer: Metal scrap
Supplier: Salt and salt chemicals, sodium chloride (salt), sodium sulphate, sodium sulfite, sodium bisulfite, sodium carbonate (soda ash), sodium metabisulphite
