Proteinase K CAS NO 39450-01-6
Lyophilized Powder from Tritirachium album limber.
CAS No. 39450-01-6
Molecular Formula: C29H27N2O12P
Molecular Weight: 626.504641
EINECS NO. :254-457-8
Other name: CHLOROM; IUB:3.4.21.14; IUB: 3.4.21.64; EC 3.4.21.14; EC 3.4.21.64; ENDOPEPTIDASE K; 3.4.21.14; PROTEINASE K.
Protease k is a highly active protease of the subtilisin class, used for the general degradation of proteins in biological samples. It is purified from Tritirachium album limber. Protease K is a serine protease with broad cleavage activity. It cleaves the carboxy terminal peptide bond of aliphatic amino acid and aromatic amino acid. This enzyme is purified to remove RNase and DNase activities. Since proteinase K is stable in urea and SDS, it also has the ability to degrade natural proteins.
Application:
1.Gene Diagnostic Kit
2.Genomic DNA extraction kit
3.RNA extraction kit removes nucleases from DNA and RNA preparation
4.Extract non-protein components from tissues to degrade protein-containing impurities-such as preparation of DNA vaccines and heparin
5.Preparation of chromosomal DNA for pulse electrophoresis
6.Western Blot
7.The general working concentration of proteinase K is 50-100ug / ml
Proteinase K
Source: Tritirachium album limber
Appearance: white lyophilized powder
Specific activity: > 30 U / mg protein (measured 33-36U / mg, subject to the current batch of COA).
Molecular weight: 29kDa (SDS-PAGE)
Definition of activity unit: at 37 and pH 7.5, the substrate casein can be hydrolyzed to produce 1 �¼mol of tyrosine eggs per minute
The amount of white enzyme K is defined as one unit (U).
Residue of DNase: Digested with �» DNA as the substrate for more than 6 hours at 37 , no deoxyribonuclease activity was detected.
RNase residue: Digested at 25 for more than 16 hours, no ribonuclease activity was detected.
Inactivation method: adding benzenesulfonyl chloride (PMSF) or diisopropyl fluorophosphoric acid (DFIP) inhibitor to the reaction system
Alive, incubation at 65 for 10-15min can partially inactivate.
Packing: 100g / bottle; 500g / bottle; 1Kg / bottle
Storage:
The dry powder state can be stored for a long time under the condition of -20 . After dissolving, dispense into appropriate volume, store at 2-8 for short-term, and -20 for long-term.
Under the correct storage conditions, the validity period of the dry powder state can reach one year. The liquid state is valid at -20 for half a year. After opening the package, if It is recommended to filter and sterilize or add a stabilizer such as 0.5% sodium azide to prevent microbial contamination if it is left at 2-8 for more than a week.
Storage and dilution buffer: 50 mM Tris-HCL buffer, 2 mM CaCL2 (pH 8.0).
Reaction conditions: Incubate for one hour at 37-56 in reaction buffer
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Name: 2-hydroxy-6-naphthoic acid Alias: 2,6 acid; 2-naphthol-6-carboxylic acid; 6-hydroxy-2-naphthoic acid; 6-hydroxy-2-naphthoic acid CAS NOï¼?16712-64-4 Molecular formula: c11h7o3 Molecular weight: 187.172 Product quality standard: Appearance characte Light yellow powder Content 99% 2-naphtholâ?¤0.10% 2,3-acidâ?¤0.10% melting point243-245�°C Loss on dryingâ?¤0.30% heavy metalâ?¤20ppm Application: used in medicine, liquid crystal, paint, etc. Packing: 25kg / cardboard barrel, or according to customer requirements
Product Name: Sodium permanganate Use level: Industrial Content: 40 (%) CAS Number: 10101-50-5 Molecular formula:NaMnO4 Molecular Weight: 141.93 Appearance: purple liquid. A density of 1.36 ~ 1.39 g / cm3, soluble in water, ethanol and ether, soluble in liquid ammonia. Decomposition in alkali. Strong oxidizing. And chemical properties similar to potassium permanganate. Purposes: as oxidants, disinfectants, fungicides and phosphorous antidotes. Toluene is also used to manufacture saccharin, o-toluene sulfonamide and organic synthesis and purification of phenol wastewater treatment. Can be used for circuit boards, metal surface cleaning, the conductive film cleaning to remove grease, electrolytic manganese dioxide, chemical fiber finishing, electroplating addition to hydrogen sulfide and other odors, as well as water treatment and so on. Packaging and storage: Industrial Pails barrel, net weight 25kg plastic drum, 250kg steel-plastic composite barrels, 1250kg IBC barrels packaging. Store in a cool dry place. Quality Standard: Â Items index Sodium permanganate (NaMnO4) Content / (%) 40 Water-insoluble content / (%) 0.01 Potassium (K) content / (%) 0.22 pH value 6 ?8 Density / (g/cm3) 1.36 1.39 Chloride (Cl) content / (%) 0.02
Product Name:Aluminum sulfate Molecular Formula:AL2(SO4)3 Hs Code:2833220000 CAS Code:10043-01-3 Standard:HG2225-2010 Shape:flake, powder, 2-4cm lump and 2-5mm/3-8mm granular Properties:There are two types,anhydrous aluminium sulphate and aluminium sulphate octadecahydrate. In normal condition , it is AL2(SO4)3�·18H2O; AL2(SO4)3�·14H2O after efflorescence, can be white powder , glittering flaky or crystalline flakes, odorless, sweet to slight bitter. Use:Sewage Treatment, treatment of drinking water, Sizing of paper , Cement Additive. Specifications Item name Standard AL2O3 15.6% 16.5% 17% Fe2O3 0.5 %  0.005 % 0.004% PH value 3.0 3.0 3.0 Water insoluble matter 0.2% 0.04% 0.03% Packing 25kg or 50kg or 1000kg in plastic lined woven bag
Product Name:Aluminium sulphate Molecular Formula:AL2(SO4)3 Hs Code: 2833220000 CAS Code:10043-01-3 Standard:HG2225-2010 Shape:flake, powder, 2-4cm lump and 2-5mm/3-8mm granular Properties:There are two types,anhydrous aluminium sulphate and aluminium sulphate octadecahydrate. In normal condition , it is AL2(SO4)3 18H2O; AL2(SO4)3 14H2O after efflorescence, can be white powder , glittering flaky or crystalline flakes, odorless, sweet to slight bitter. Use:Sewage Treatment, treatment of drinking water, Sizing of paper , Cement Additive. Specifications Item Name Standard AL2O3 15.6% 16.5% 17% Fe2O3 ¤0.5 % 0.005 % 0.004% PH value .0 3.0 3.0 Water insoluble matter 0.2% 0.04% 0.03% Packing 25kg or 50kg or 1000kg in plastic lined woven bag
4-Hydroxybenzoic acid Chemical Name:4-Hydroxybenzoic acid Trade Name:p-oxybenzoic acid Molecular Formula:C7H6O3 Molecular Weight:138.12 CAS No.:99-96-7 Properties:white odorless crystalline powder, easily soluble in hot water and alcohols, aethers and acetone, slightly soluble in cold water and benzene, insoluble in carbon bisulfide; relative density is 1.46 Use: Can be used as antiseptic, intermediate of dyes, pharmaceuticals; as main material of liquid crystal polymers; as material of pesticide intermediate Specification Item Specification Polymer Grade Technical Grade Appearance white crystal powder white crystal powder Purity(on dry base) % 99.6 99.0 Melting Point 214-217 212-216 Odor odorless odorless Solubility Clear and transparent clear and transparent Loss on Drying % 0.20 0.50 Color(Pt-Co) 10 40 Ash % 0.02 0.15 Sulfate(SO42-) 0.01 0.05 Chloride(Cl-) % 0.005 0.02 Phe% 0.01 0.10 Salicylic Acid % 0.02 0.10 4-Hydroxyisophthalic Acid(HIPA) ppm 500 / Insolubles in Metha ppm 50 / Potassium(K+) ppm 5 / Sodium (Na+) ppm 5 / Iron (Fe) ppm 5 / Ca2++Mg2+ppm 5 /
English name: Calcium Nitrite Molecular formula: Ca(NO2)2 Molecular weight: 132 CAS NO. 13780-06-8 HS CODE: 28341000 UN NO.: 2627 5.1 type oxidizing agent Properties: White powder without crystal water. It is tasteless and toxic. Easily in water and presents light yellow solution. Specifications: Item Superior grade First grade Second grade Calcium Nitrite[Ca(NO2)2 as dry basis]% 94 92 90 Calcium Nitrate[Ca(NO3)2 as dry basis]%
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