The structure and function of the protein need to be maintained in a suitable pH environment. Therefore, the choice of pH buffer is very important during the preparation and purification of the protein. Only a suitable buffer can ensure the activity and structural stability of the protein. Effect of buffer in protein purification process There are many kinds of buffers for protein purification. While maintaining the pH of the system, it is necessary to ensure that the protein cannot be inactivated during the purification process, or to minimize the amount of protein inactivation, which must be maintained in every step of protein purification. Solubility and activity of protein. Protein purification buffer requirements The buffer in the protein purification process should not only prevent protein degradation, but also prevent protein aggregation, which has a significant impact on the experimental results. The following factors should be considered when selecting and reagent buffers: pH, buffer system, salt ions, reducing agents and stabilizers. Each of these factors should be optimized according to the purified target protein, and the activity of the target protein should be used as an evaluation criterion for optimization. Understand protein stability conditions There are many types of proteins, and different proteins adapt to different pH values. The buffer should be selected according to its optimal pH value. In many cases, the pH is 7.4, which is a mimic biological condition, and most proteins are stable at this pH. However, there are some exceptions, which require pH adjustment to keep the protein soluble and not degraded. Commonly used buffers for protein purification Commonly used buffers in the protein purification process include Tris, Tricine, Bis-Tris, MOPS, etc. Among them, Tris is commonly used. Its buffer range is close to physiological pH and has a low ionic strength. It is a good solvent for proteins. Can ensure the stability of the protein. It should be noted that when preparing the buffer, try to avoid the isoelectric point pl of the target protein. Because the protein has no electrostatic charge on its surface in the pH solution of its isoelectric point, the repulsive force between the hundred molecules is reduced, it is easy to aggregate, and the solubility is reduced. Now the adaptation pH value, isoelectric point, and buffer range of the buffer to be purified can be found, and you can directly match it according to the corresponding requirements. Desheng is a manufacturer of in vitro diagnostic reagents, which can provide large-package pH buffers such as Tris, MOPS, Bicine, etc.
-White Charcoal, also known as Binchotan, is a type of hardwood charcoal that has traditionally been produced in Japan. It is called �¢??White�¢?? charcoal because of its light color and unique benefits that create economic value compared to traditional black charcoal. -Binchotan is the purest charcoal on earth by the highest carbon proportion (more than 92%) and is completely odorless, and smokeless when burning. White Charcoal is more difficult to ignite than black charcoal; however, it burns hotter and longer. When hitting the pieces together, it gives a light metallic sound. The white charcoal doesn�¢??t break into small pieces while burning. USAGES Food grilled over white charcoal retains its natural flavor and moisture because it burns cleanly and without flames. It is popular in high-end restaurants and mostly in Korea and Japan. In addition, binchotan has many uses such as water purification, exhaust filter, deodorant, skin care, metallurgy, used in battery manufacture...
The mentha water is steam distilled from fresh blossoms Bulgarian mentha piperita of during the months of may and June of each year. It is a completely natural product for skin and hair application. Transparent liquid, colorless; specific aroma; essential oil content 0.03, ethyl alcohol content 3.44, relative density 20 C 0.9827, PH 5.25, microbiological purity - corresponds to standard. Benefits: Has an antiseptic and anti-inflammatory effect on the skin. Soothes the skin after sunburns or razor cuts. Gently cleanses and tightens the pores of the oily skin. Moisturizes the skin and improves its general condition.
Rose alba ( white rose) essential oil is made by alba groups ltd. The Rosa alba has a much more intense aroma than Rosa damascena, source of our traditional rose Otto. Rose oil made from rose alba is much more expensive than the rose Otto made by rose damascena because in comparison with rose damascena for 1 kg rose oil we need two times more rose flowers to produce 1 kg rose alba oil. Rose alba essential oil controls & balances sebum production therefore, useful for both dry & oily skin. Rose alba essential oil assists in balancing & restoring the skin's pH and helps tighten pores. Rose alba essential oil is a wonderful skin toner.
High quality fine graded product with a well balanced pH.
Wheat (food grade, for human) origin Ukraine high protein protein not less 13% clean in pp bags 25/50/1000 kg
Glassiness - 80-85% Protein - 16% Gluten - 27% Trash - 0,5-1% Moisture - 10,5%
Coco peat/coir pith, buffered cocopeat, coco chips, coconut fibre.Manufacture and exporter
Nowadays growing worldwide interest to flax seeds as a useful foodstuff. Flax seeds are rich in proteins, fats, gluten and dietary fiber. Each of these components contributes to a healthy human nutrition. Flax seeds can be added to homemade cakes, used for yoghurts, cereals and salads. Flax seeds contain plentiful number of components that can help to protect the human body from cardiovascular, cancer and other diseases. The main active substances contained in flax seed are: proteins, polysaccharides; vegetable fibers (lignans); polyunsaturated fatty acid (a-linolenic and others), vitamins A, B, E, F. Flax seeds Colour: brown Origin: Ukraine Purity: 98-99.9% Moisture - 8% max Our company deals with direct manufacturers /wholesalers of flax seed, which is grown and processed in the agrarian farms of Ukraine and Russia.
Peas are a high-calorie product, rich in protein, minerals and vitamins content. Besides, its health benefit is proved by the high content of antioxidants, important for keeping skin beauty and vibrancy. We supply yellow peas for human consumption, conservation, cereals and fodder production. Admixture 2% Moisture 12% Broken 8.2% Damaged 1% Bag damage 0.8% Packing details: BULK, 25kg, 50kg PP bag No. Denomination of characteristics Description and the norms for the first sort 1. Colour Yellow, green 2. Taste Normal, peculiar to pea, without foreign after-taste, not sour, not bitter 3. Odor Normal, peculiar to pea, without musty or mouldy odor or other foreign odor
Black cumin is a part of the buttercup family and the seeds are dark, thin, and crescent-shaped when whole. The seeds have been used for many centuries in the Middle East, the Mediterranean and India. Today, black cumin seeds are used as a seasoning spice in different cuisines across the world due to their nutty flavor. Besides their culinary uses, black cumin seeds also have a wealth of important health benefits and are one of the most cherished medicinal seeds in history. The seeds of the black cumin plant contain over 100 chemical compounds, including some yet to be identified. In addition to what is believed to be the primary active ingredient, crystalline nigellone, black cumin seeds contain: thymoquinone, beta sitosterol, myristic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachidonic acid, protein, vitamin B1, vitamin B2, vitamin B3, folic acid, calcium, iron, copper, zinc, and phosphorous.
The Cashew tree (Anacardium Occidentale) is a tropical evergreen native to the Americas but is now widely cultivated in Asia and Africa. Cashew in its natural form is a soft, white, meaty kernel contained within the hard shells of kidney shaped, raw cashew nuts. Cashew is consumed all over the world as a snack or used as a food ingredient. Every year, the world eats more than 600,000 metric tons of cashew. And fortunately, it’s a very healthy food. Cashew nuts are among the most nutritious nuts in the world. Cashew Nuts are low in fat, have zero cholesterol, are high in protein, and include a large amount of important vitamins and minerals. Every single cashew nut comes from its own cashew apple, which are grown on cashew trees and thrive in tropical climates. The nuts are then collected, dried, shelled, peeled, graded, and roasted.
Peanuts are popularly used as seed oil in India. They are easily available round the year and almost everywhere in India. They are easily available round the year and almost everywhere in India. Peanuts are also taken as snacks in many households especially in India. In reality, peanuts are actually legumes. But since they have all the properties of nuts like almonds, cashew nuts, etc., these are also included in the family of nuts. Groundnut/Peanuts are known as Moongphali in India. India is one of the largest producers of groundnuts in the world. The special variety peanut of this area (Saurastra, Gujarat) is world famous in its flavor, taste and aroma. Raw Groundnut valued for its protein contents, which is of high biological value. Groundnut contains more protein then meat, two and half more than eggs and more than any other vegetable food. It also contains phosphorous, thiamin and niacin that help in building resistance against all infections, such as Hepatitis and tuberculosis. We can offer Groundnut/Peanuts Peanuts Long Type (Bold Type): Long/Bold Type 38-42, 40-50, 50-60, 60-70, 70-80, and Crushing Grade Quality Counts/Ounce Peanut Round Type (Java Type): Round Type(Java Type) 40-50, 50-60, 50-60, 60-70, 70-80, 80-90, 90-100, 140-160 Counts/Ounce Jute Bags Packing: - We shall be able to provide in 5Kg, 10 kg, 15 Kg, 25 kg and 50 Kg jute bags packing. Vacuum Packing: - For special requirements for our buyers, we can provide the cargoes in 10 kg, 12.5 kg and 25 kg vacuum packed PP bags
Turmeric powder is a bright yellow powder made by dry grinding of mature turmeric rhizomes (underground stems). The use of turmeric for coloring and flavoring food, for cosmetic purposes and for medicinal properties dates back to the ancient Vedic culture of India. Used in almost all Indian curries, this spice has almost no calories (1 tablespoon = 24 calories) and zero cholesterol. It is rich in dietary fiber, iron, potassium, magnesium and vitamin B6. Health Benefits of Turmeric The wide range of turmeric health benefits come mainly from its main ingredient, curcumin. This widely researched component of turmeric is highly therapeutic and is used in various drugs and pharmaceutics mainly because of its immunity boosting and anti-oxidant properties. Boosting Immunity – Curcumin has a huge therapeutic value and boosting immunity is one of the most important properties of curcumin. “5 to 8 times stronger than vitamin E and stronger than vitamin C, this antioxidant breakthrough may help boost your immunity, maintain normal cholesterol levels, and put the brakes on aging Anti-inflammatory and Anti-oxidant Property – Free radical causing oxidative damage of DNA and proteins are associated with a variety of chronic diseases such as cancer, atherosclerosis, and neurodegenerative diseases. Curcumin plays an important role in curbing these conditions.
Castor meal is also variously called castor meal, castor residue, castor extract & de-oiled castor cake Castor meal - the residue obtained from castor cake by the solvent extraction process - is one of the most versatile natural manures. It is truly organic manure which enhances the fertility of the soil without causing any damage or decay. It is enriched with the three big elements vital and conducive to the proper growth of crops - Nitrogen, Phosphorus and Potassium. It also has traces of nutrients like Manganese, Zinc and Copper, thus making it a balanced fertilizer. Moreover, it helps to neutralize the detrimental effects of chemical fertilizers. Apart from their contribution to Nutrients, they have a number of benefits in agriculture, which none of the synthetic fertilizers or pesticides can offer. They bring in the wonderful molecules that nature has designed to help the plants flourish naturally. They provide slow and steady nourishment, stimulation, protection from soil nematodes and insects; improve yields, and quality of product like taste, flavour, amino acid composition etc. The pressed cake obtained after the expression of castor bean. The solvent extracted cake, although rich in protein cannot be used as cattle fodder because of its toxicity. However, it can be used as a fertilizer. The protein content of castor seed meal varies from 21-48% depending upon the extent of decortications. It has an ideal amino acid profile with moderately high Cystine, mithionine, and isoleucine. But its ant nutritional substances, ricin, ricine and an allergen restrict its use in poultry feed, even at a very low level of inclusion. Castor Cake is an excellent fertilizer because of high content of N (6.4%), Phosphoric Acid (2.55%) and Potash (1%) and moisture retention. There is negative correlation between the contents of ricinine in castor seeds and oil content (r = -0.76). This content of ricinine in castor seed is determined by Agro climatic conditions. Nutrient content of Castor Cake: Organic matter - 75 - 80 %, Nitrogen - 4.0 - 4.5 %, Phosphorous - 1.5 %, Potassium - 1.25 - 1.5 %. It also contains some micro nutrients viz., Calcium, Magnesium, Sulphur, Iron, Zinc, Manganese, Copper etc. A typical composition of castor residue/meal is as follows: Nitrogen - 4% min. approx. Phosphorous - 1% min. approx. Potassium - 1% min. approx. Moisture - 10 - 12 % max. approx. Oil Content - 0.7% max. approx. Advantages: 1. It provides all the major & minor nutrients necessary for better plant growth. 2. It also helps in increasing the nutrient uptake by plants. 3. It improves the soil fertility and productivity. 4. It is known to protect the plants from nematodes and termites. 5. It improves the yield & quality of the farm produce. 6. It improves the physical, chemical and biological properties of soil
Applications: In Animal feed stuff Nutrition feed for Poultry, Layers, Cattle. Primarily used as a dairy cow feed supplement, the buffering capability of Natural Soda's pure and natural feed grade sodium bicarbonate helps stabilize rumen pH by reducing acidic conditions. Improves calcium absorption and eggshell quality of laying hens during peak production.
Ginger root Botanical name: Zingiber officinale Linn. Family: Zingiberaceae. Ginger oil and oleoresins are the volatile oil derived by steam distillation of ginger and oleoresin. It is obtained by percolating the powdered rhizomes of Ginger, Zingiber officinale with volatile solvents. Ginger contains 1-2 percent of volatile oil, 5-8 percent of pungent acrid oleoresin and starch. Zingiberene is the chief constituent in the oil of ginger. Oil is employed for flavoring all kinds of food products and confectionary and finds limited use in perfumery. Oleoresin, commercially called Gingerin contains pungent principles viz. gingerol and shogaol apart from the volatile oil of ginger and is used as an aromatic, carminative, stomachic and as a stimulant. Oleoresin from ginger is obtained conventionally by extraction of dried powdered ginger with organic solvents like ethyl acetate, ethanol or acetone. Commercial dried ginger yields 3.5-10.0 per cent oleoresin. Ginger oleoresin is a dark brown viscous liquid responsible for the flavour and pungency of the spice. Ginger of commerce or `Adrak` is the dried underground stem or rhizome of the plant, which constitutes one of the five most important major spices of India, standing third or fourth, competing with chillies, depending upon fluctuations in world market prices, world market demand and supply position. Ginger, like cinnamon, clove and pepper, is one of the most important and oldest spices. It consists of the prepared and sun dried rhizomes known in trade as `hands` and `races` which are either with the outer brownish cortical layers (coated or unscraped), or with outer peel or coating partially or completely removed. Ginger requires a warm and humid climate. It is cultivated from sea level to an altitude of 1500 meters, either under heavy rainfall conditions of 150 to 300 cm or under irrigation. The crop can thrive well in sandy or clayey loam or lateritic soils. The composition of dry ginger is given below: Dry Ginger rootMoisture:6.9 % Protein:8.6 % Fat:6.4 % Fiber:5.9 % Carbohydrates:66.5 % Ash:5.7 % Calcium:0.1 % Phosphorous:.15 % Iron:0.011 % Sodium:0.03 % Potassium:1.4 % Vitamin A:175 I.U./100 g Vitamin B1:0.05 mg/100 g Vitamin B2:0.13 mg/100 g Niacin:1.9 mg/100 g Vitamin C:12.0 mg/100 g Calorific value:380 calories/100 g. Ginger Oleoresin is obtained by extraction of powdered dried ginger with suitable solvents like alcohol, acetone etc. Unlike volatile oil, it contains both the volatile oil and the non-volatile pungent principles for which ginger is so highly esteemed. Concentration of the acetone extract under vacuum and on complete removal of even traces of the solvent used, yields the so called oleoresin of ginger. Ginger oleoresin is manufactured on a commercial scale in India and abroad and is in great demand by the various food industries.
Applications: Food ingredients for bakery, beverage, confectionery industry It is used as a raising agent in biscuits, cookies, bread, cakes, farsan, jaggery & confectionery. It acts like a buffer and carbonation agent in beverages, electrolyte and rehydration solutions.
The primary use of Ammonium Sulphate is as a fertilizer for alkaline soils. In the soil the ammonium ion is released and forms a small amount of acid, lowering the pH balance of the soil, while contributing essential nitrogen for plant growth. The main disadvantage to the use of Ammonium Sulphate is its low nitrogen content relative to Ammonium Nitrate, which elevates transportation costs. It is also used as an agricultural spray adjuvant for water soluble insecticides, herbicides, and fungicides. There it functions to bind iron and calcium cations that are present in both well water and plant cells. It is particularly effective as an adjuvant for 2,4-D (amine), glyphosate and glufosinate herbicides.
Durum Wheat Origin: Argentina Crop: 2017 Vitrio: 60% min PH: 78-79 Protein: 11.5- 13% Soft wheat: 5% max
