Ncov Multiplex One Step Rt Qpcr Probe Kit Suppliers and Manufacturers

Multiplex One Step RT-qPCR Probe Kit is a multiplex quantitative PCR kit based on RNA as template. In the process of the experiment, reverse transcription and quantitative PCR were carried out in the same tube, which simplified the experimental operation and reduced the risk of contamination. In this kit, the first strand cDNA was efficiently synthesized by heat-resistant Hifair V reverse transcriptase and quantitatively amplified by UNICON HotStart Taq DNA polymerase. The kit mainly contains optimized MP buffer, enzymes mix, etc. The buffer solution already contains Mg2+ and dNTP. In addition, the factors that can effectively inhibit the non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reactions are added, which can ensure the amplification efficiency and carry out up to four reactions. Quantity: 100 T, 1000 T, 10000 T Applications: RT-qPCR, 4-plex reaction Sample Type: RNA template main enzyme: Hifair V Reverse Transcriptase,UNICON HotStart Taq DNA Polymerase Category: IVD PCR Research Area: RT-qPCR

CE,ISO 13485 Novel Coronavirus (COVID-19) Nucleic Acid Detection Kit (RT-PCR) is used for nucleic acid detection of the ORF1ab sequence and N gene of the Novel Coronavirus (SARS-CoV-2) in nasopharyngeal swab, oropharyngeal swab, sputum and other specimens. The primer and probe sets in this kit are designed for the detection of the conserved sequences of SARS-CoV-2 (ORF1ab and N gene). The kit also contains an Internal Control (human RNase P gene) that is used to assess specimen quality and avoid a false negative result. Real-Time PCR technology utilizes polymerase chain reaction (PCR) for the amplification of specific target sequences and target specific probes for the detection of the amplified RNA. The probes are labelled with fluorescent reporter and quencher dyes.

WE CAN PROVIDE YOU PCR KIT AT PRICE: $1.20/KIT IN BULK QUANTITY VALIDITY: DECEMBER 2023 PRICE: FOB/ CIF (AVAILABLE) QUANTITY AVAILABILITY: 400,000 PCS

PERFORMANCE SPECIFICATIONS 1. Detection limitation: 200 copies /mL. 2. Precision: using precision reference CV1 and CV2 for within-batch and between-batch detection, the coefficient of variation (CV) of their Ct values is â?¤ 5.0%. 3. Conformity rate of Negative Control: 100% 4. Conformity rate of Positive Control: 100% 5. Specificity: This product will not cross react with positive samples of human coronavirus HKU1 OC43, NL63 and 229E, SARS coronavirus, MERS corona-virus, H1N1 (new influenza A (H1N1) virus (2009), the seasonal H1N1) and H3N2, H5N1, H7N9, influenza B Yamagata, Victoria, respiratory syncytial virus type A, B, parainfluenza type 1, 2, 3, rhinovirus species A, B and C, adenovirus, 1, 2, 3, 4, 5, 7 and 55, enterovirus species A, B, C, D,Human pulmonary virus, partial pulmonary virus, EB virus, measles virus, human cytomegalo virus, rotavirus, norovirus, mumps virus, varicella-zoster virus and mycoplasma pneumoniae, chlamydia pneumoniae, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, pyogenic streptococcus, klebsiella pneumoniae, mycobacterium tuberculosis, pyogenic streptococcus, candida albicans, candida glabrata, newborn Cryptococcus and human genome DNA.

Hi-PCR Coronavirus (COVID-19) Multiplex Probe PCR Kit Product Details: Number of Reactions(Preps)/Kit : 100 Rxns Per Box Brand : HIMEDIA Storage Temperature : -10 to -20 C Detection : Probe Based Target : E, N and RdRp gene. Cat No : MBPCR243 Description A ready to use Rt-PCR Kit for Detection of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing coronavirus disease 2019 (COVID-19). SARS-CoV-2 is a coronavirus, subgenus Sarbecovirus, 150-200nm in diameter with a genome size of about 30 Used For A ready to use Rt-PCR Kit for Detection of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing coronavirus disease 2019 (COVID-19).

Description This kit utilizes fluorescence quantitative probe-based PCR and guarantees a high specificity to ensure accurate one-step identification of ORF1ab and N genes of 2019-nCoV. The kit offers a highly sensitive test with a limit of detection as low as 500 copies/ml. The results are available within 1.5 hour. Kit Contents (48 Tests /Kit) Dual Enzyme solution A 50 µl Dual Ncvo-O / N reaction solution A 1 ml Dual Ncvo-O / N positive control A 200 µl Dual Negative control A 200µl Applicable equipment ABI series, Bio-Rad, Roche series and other fluorescent real-time PCR instruments. Specimens requirements Specimen type: nasopharyngeal swab from suspected infection; virus cell culture fluid, etc. Basic Protocol 1. Sample preparation. Take the test sample and extract the RNA nucleic acid according to the instructions of the nucleic acid extraction kit. The nucleic acid extraction product should be stored at -20 C. 2. Reaction mixture preparation According to the total number of test samples, the number of PCR reaction tubes needed is. N = number of samples + 1 negative control + 1 positive control. The following protocol is recommended for a 20 µl reaction volume. If the volume of reaction changes, please adjust proportionally. Components Volume Dual Enzyme solution A 1 µl Dual Ncvo-O / N reaction solution A 19µl Final Volume: 20 µl 3. Sample adding. Add 5 µl each of the extracted RNA, positive and of negative control to corresponding reaction tubes. After assembling all the components, cover the tube caps and gently mix the contents of the tube, mix well, and centrifuge briefly. 4. Perform quantitative PCR Place the reaction tube inside a real-time PCR instrument. Set the channel and sample information, reaction system volume 25 µl. Select the following channels: FAM channel for nCOV-ORF1ab, VIC channel for nCOV-N. Perform quantitative PCR using recommended cycling parameters settings: Step Temperature Time Number of cycles Fluorescence Detector 1 50C 15 min 1 Off 2 95C 3 min 1 Off 3 95C 10sec 45 Off 60C 30 sec On Result analysis: 1) Set the baseline: Generally, it is set to 6-15cycle for ABI 7500, 7700 and other instruments, 3-15cycle for PE5700, and 6-12cycle for MJ Research Option2. Under special circumstances, the baseline can be adjusted accordantly. 2) Set the threshold: The threshold line just exceeds the highest point of the negative control amplification curve (random noise line).

Our test kit has been approved and endorsed by Singapore's MOH (Ministry of Health) and HSA (Health Science Authority) since last year and we have been constantly supplying to the APAC countries during this period. (Japan, India, Singapore, Malaysia, Indonesia and etc.) * CE mark was attained in December 2020. * Our Tepat 1.0 SARS-CoV-2 RT-PCR provides for high-throughput, sensitive and specific detection of SARS-CoV-2 RNA extracted from nasopharyngeal swabs*. It can detect 6 copies of viral RNA per reaction with > 95% confidence. Clinical validation shows 100% sensitivity, with no cross-reaction with non-SARS-CoV-2 species. Key Highlights 1.5 hours from RNA to results Multiplex RdRp and M viral genes detection enhances sensitivity and specificity 100% coverage with 100% identity of all SARS-CoV-2 genomes in the world Detects 6 copies of viral RNA Whole process control: human RNase P

STANDARD M nCoV Real-Time Detection kit is used for rapid identification and detection of novel coronavirus (2019-nCoV) nucleic acids in human nasopharyngeal swabs and throat swab samples. - One tube reaction for identification and detection of 2019-nCoV - One-step Real-Time RT-PCR - Provide all reagents required for PCR - Designed according to WHO interim guidance for laboratory testing for 2019 novel coronavirus (2019-nCoV) in humans" - nCoV primers/probes ORF1ab (RdRp) gene, E gene - Provide Internal controls - Test time : 90mins - Specimen : Nasopharyngeal swab, Oropharyngeal swab, Sputum - Applicable machine : CFX96. ABI7500 - CE, FDA EUA

The COVID-19 RT-PCR Detection Kit is a real-time RT-PCR test intended for the qualitative detection of nucleic acid from the SARS-CoV-2 in upper and lower respiratory specimens (such as anterior nasal swabs, mid-turbinate nasal swabs, nasopharyngeal swabs, oropharyngeal swabs, sputum, lower respiratory tract aspirates, bronchoalveolar lavage, and nasopharyngeal wash/aspirate or nasal aspirate) from individuals suspected of COVID-19 by their healthcare provider. Testing is limited to laboratories certified under the Clinical Laboratory Improvement Amendments (CLIA) of 1988, 42 U.S.C. 263a, to perform high complexity tests. Results are for the identification of SARS-CoV-2 RNA. The SARS-CoV-2 RNA is generally detectable in upper and lower respiratory specimens during the acute phase of infection. Positive results are indicative of the presence of SARS-CoV-2 RNA; clinical correlation with patient history and other diagnostic information is necessary to determine patient infective status. The agent detected may not be the definite cause of disease. Positive results do not rule out bacterial co-infection with other viruses. Laboratories within the United States and its territories are required to report all positive results to the appropriate public health authorities. Negative results do not preclude SARS-CoV-2 infection and should not be used as the sole basis for patient management decisions. Negative results must be combined with clinical observations, patient history, and epidemiological information. The COVID-19 RT-PCR Detection Kit is intended for use by qualified trained clinical laboratory personnel specifically instructed and trained in the techniques of real-time PCR and in vitro diagnostic procedures. The COVID-19 RT-PCR Detection Kit is only for use under the Food and Drug Administration's Emergency Use Authorization. PRINCIPLE OF DETECTION This product is a fluorescent probe-based Taqman RT-PCR assay system. Firstly, the RNA of SARS-CoV-2 will be reverse transcribed into cDNA by reverse transcriptase, and then PCR amplification will be performed with cDNA as template. During amplification of the template, the TaqMan probe will be degraded due to the 5'-3 polymerase activity and exonuclease activity of Taq DNA polymerase, then the separation of fluorescent reporter and quencher enables the fluorescent signal to be detected by instrument. The ORF1ab gene of SARS-CoV-2 will be detected qualitatively by FAM channel, the N gene of SARS-CoV-2 will be detected qualitatively by JOE channel, the E gene of SARS-CoV-2 will be detected qualitatively by ROX channel, and the internal reference will be detected by CY5 channel. dUTP and UNG enzyme are used in the kit to prevent contamination of the amplified products. Internal reference is used in the kit for quality control starting from sample collection

The KAiRA 2019-nCoV PCR Detection Kit is a one step RT-PCR reagent system, based on real-time PCR technology, for the qualitative detection and differentiation of lineage B-beta Coronavirus and severe acute respiratory syndrome coronavirus 2 specific RNA. The test kit has FDA and CE Approval and comes in packs of 100 tests. In combination with Dr PCR the test results for PCR Testing can be given in under an hour and are compatible for use for international travel requirements. Unit - Kit (1 kit contains 100 tests) Unit Price - $900 per kit

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Super long life refrigeration sheet modified by special process; Precision temperature control technology ensures that the temperature fluctuation of each hole is less than 0.1 ; Ultra high sensitivity PMT system combined with precision optical system ensures accuracy; New automatic hot cover technology can effectively prevent reagent evaporation; Maximum 6 channels fluorescence detection, no cross interference between channels; Intelligent operating system, flexible program setting, comprehensive analysis and report function;

- In vitro diagnostics(IVD) Kits - Designed for the qualitative detection of COVID-19 viral RNA - Oropharyngeal swab, nasopharyngeal swab, sputum and bronchoalveolar lavage (BAL) - Store at -25~ -15 Product description: Detects the novel coronavirus. COVID-19 using one-step real-time RT-PCR in a single tube. The target genes for the detection of the virus are Orf1ab and N gene. The sequence of primers and probes for the amplification were adapted from sources published by China CDC and WHO. The kit includes primers and probe for the amplification of the human RNase P gene that serves as an internal positive control for the real-time RT-PCR. Compatible real-time PCR machines: CFX96(Bio-Rad), ABI 7500/7500Fast(Thermo Fisher Scientific), SLAN-96S(Hongshi), Rotor-Gene Q(QIAGEN)

Product Description : The PaxView COVID -19 real-time RT-PCR Kit detects the novel coronavirus, COVID-19 using one-step real-time RT-PCR in a single tube intended for in vitro diagnostic use. The target genes for the detecton of the virus are Orf1ab and N gene. The sequence of primers and probes for the amplification were adapted from sources published by China CDC and WHO. The kit includes primers and probe set targeting the human RNase P gene that serves as an internal positive control for the real-time RT-PCR. Price of product ( USD price or FOB price) : 1no testing 20 USD 1kit testing = 96no testing 1kit = 96no * 24 USD = 2208 USD (base on Qty negociable) Product origin ; korea Key Specifications/Special Features : 1. with in 3hour testing result in come 2. One item 96person can check 3. 100% correct result coming then other method 4. It is same process in korea Minimum Order Size and Packgaing details : 50kit in box 500 x 460 x350 6kg MOQ : 500

Cat No : 11NCo10. SD biosensor STANDARD M nCoV Real-Time Detection kit is used for identification and detectionof novel coronavirus (2019-nCoV) ORF1ab (RdRp) gene and E gene in humannasopharyngeal swab, oropharyngeal swab, and sputum specimens using reversetranscription (RT) real-time PCR. This kit is helpful for the auxiliary diagnosis of 2019novel coronavirus disease (COVID-19). The test results are for clinical referenceonly and cannot be used as a basis for confirming or excluding cases alone.

Pathodetect 2019- nCov Detectioin kit offer an in virto diagnostic real time PCR assay for qualitative detection of 2019 - Novel Coronavirus RNA in respiratory specimens and sera. The kit offer the universal detection of SARS- Like coronavirus and specific detection of 2019-nCOv. Mylab Discovery solutions 2019-nCoV Detection Kit provides ready to use solution for detection of coronavirus using real time PCR technology. Detection is based on the amplification of a specific conserved RNA sequences of coronavirus and detection by Taqman probe-based chemistry. An Internal control is detected along with the coronavirus target controlling the extraction efficacy and possible PCR inhibition. The test is designed to use across wide range of PCR platforms. The recommended CDC protocol for coronavirus detection ensures that the kit leads to highly-specific and ultrasensitive results in short time. KIT CONTENTS Reagents for Nucleic acid extraction from respiratory/sera specimens Reagents for Real time PCR Inclusion of synthetic positive and negative controls

2019-nCoV One step rapid test device Within 10 minutes you will have the test result. Very accurate and simple operation. Please contact me for the video to see how easy it can be. With CE certificate. One box can take 25 samples.

Novel Coronavirus (2019-nCoV) Real-time RT-PCR Kit is a real-time RT-PCR test intended for the qualitative detection of nucleic acid from the 2019-nCoV in oropharyngeal swabs and bronchoalveolar lavage fluid collected from individuals suspected of COVID-19 by their healthcare provider. A kit contains: Package specifications: 24 tests/kit, 48 tests/kit 1) 2019-nCoV-PCR Master Mix (Lyophilized Powder): 1 bottle/box 2) 2019-nCoV-PCR Positive Control (Lyophilized Powder):1 bottle/box 3) 2019-nCoV Diluent: 1 bottle/box 4) User Manual