Fluorescence Quantitative Pcr Instrument

Real time PCR instrument. With 16-, 48-, or 96- wells. with 4-. 5-. or 6- channels.

CFX Opus 96 Real-Time PCR Instrument 0.2 mL, 96 Wells Product Details: Brand BioRad Model Name/Number CFX Opus 96 Sample Capacity/Format 96 wells Multiplex analysis Up to 5 targets per well Dimensions (W x D x H), cm/in 33x56x36 / 13x22x14 Cat No 12011319 Description CFX Opus is the newest real-time PCR system in our robust CFX Family. The CFX Opus 96 Real-Time PCR System can analyze 96 wells and offers a number of new features: More uniform thermal performance Expanded connectivity Wi-Fi, ethernet, and USB Cloud connectivity: Operate CFX Opus on our BR.io cloud platform or with desktop CFX Maestro Software Network storage drive access for excellent data management Shuttle optical system yields consistent optical measurements across your sample plate SpecificationsThermal CyclerMaximum ramp rate, C/sec5Average ramp rate, C/sec3.3Heating and cooling methodPeltierLid, C30 110 Temperature Range, C4 100Accuracy, C ±0.2 of programmed target at 90 C Uniformity, C ±0.3 well-to-well after 10 sec of arrival at targetGradientOperational range, C30 100Programmable span, C1 24Optical DetectionExcitation6 filtered LEDs Detection6 filtered photodiodes Range of excitation wavelengths, nm450 684 Range of emission wavelengths, nm510 730Sensitivity Detects 1 copy of target sequence in human genomic DNADynamic range10 orders of magnitudeScan TimeAll channels, sec12 FAM/SYBR Green only, sec3SoftwareOperating systemWindows 10Multiplex analysisUp to 5 targets per wellSystemSample capacity, wells96 Sample size, µl150 (10 50 recommended) Communication interface USB 2.0 Electrical approvalsIEC, CE Dimensions (W x D x H), cm/in33x56x36 / 13x22x14Weight, kg/lb22/48

The COVID-19 RT-PCR Detection Kit is a real-time RT-PCR test intended for the qualitative detection of nucleic acid from the SARS-CoV-2 in upper and lower respiratory specimens (such as anterior nasal swabs, mid-turbinate nasal swabs, nasopharyngeal swabs, oropharyngeal swabs, sputum, lower respiratory tract aspirates, bronchoalveolar lavage, and nasopharyngeal wash/aspirate or nasal aspirate) from individuals suspected of COVID-19 by their healthcare provider. Testing is limited to laboratories certified under the Clinical Laboratory Improvement Amendments (CLIA) of 1988, 42 U.S.C. 263a, to perform high complexity tests. Results are for the identification of SARS-CoV-2 RNA. The SARS-CoV-2 RNA is generally detectable in upper and lower respiratory specimens during the acute phase of infection. Positive results are indicative of the presence of SARS-CoV-2 RNA; clinical correlation with patient history and other diagnostic information is necessary to determine patient infective status. The agent detected may not be the definite cause of disease. Positive results do not rule out bacterial co-infection with other viruses. Laboratories within the United States and its territories are required to report all positive results to the appropriate public health authorities. Negative results do not preclude SARS-CoV-2 infection and should not be used as the sole basis for patient management decisions. Negative results must be combined with clinical observations, patient history, and epidemiological information. The COVID-19 RT-PCR Detection Kit is intended for use by qualified trained clinical laboratory personnel specifically instructed and trained in the techniques of real-time PCR and in vitro diagnostic procedures. The COVID-19 RT-PCR Detection Kit is only for use under the Food and Drug Administration's Emergency Use Authorization. PRINCIPLE OF DETECTION This product is a fluorescent probe-based Taqman RT-PCR assay system. Firstly, the RNA of SARS-CoV-2 will be reverse transcribed into cDNA by reverse transcriptase, and then PCR amplification will be performed with cDNA as template. During amplification of the template, the TaqMan probe will be degraded due to the 5'-3 polymerase activity and exonuclease activity of Taq DNA polymerase, then the separation of fluorescent reporter and quencher enables the fluorescent signal to be detected by instrument. The ORF1ab gene of SARS-CoV-2 will be detected qualitatively by FAM channel, the N gene of SARS-CoV-2 will be detected qualitatively by JOE channel, the E gene of SARS-CoV-2 will be detected qualitatively by ROX channel, and the internal reference will be detected by CY5 channel. dUTP and UNG enzyme are used in the kit to prevent contamination of the amplified products. Internal reference is used in the kit for quality control starting from sample collection

Supplier: Real time rt pcr detection kit (lyophilized), nucleic acid extraction kit, fluorescent pcr instrument
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This kit is used to qualitatively detect in vitro suspected cases of pneumonitis infected by a new type of coronavirus. The PCR reaction solution contains 2 sets of specific primers and fluorescent probes for specific detection of targets, and an additional set of specific primers and fluorescent probes is used as the internal standard control of the kit for detecting endogenous housekeeping genes. Lowest detection line:200copy/mL Specificity: this kit has no cross-reaction with hepatitis B virus, hepatitis C virus, staphylococcus aureus, gram-positive bacteria, shigella bacillus, influenza A, influenza B, enterovirus and human genome DNA/RNA. Precision reference products: After repeated 20 times, the amplified Ct CV of each fluorescence channel was no more than 5%.Ct CV 5%

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