PERFORMANCE SPECIFICATIONS
1. Detection limitation: 200 copies /mL.
2. Precision: using precision reference CV1 and CV2 for within-batch and
between-batch detection, the coefficient of variation (CV) of their Ct values
is â?¤ 5.0%.
3. Conformity rate of Negative Control: 100%
4. Conformity rate of Positive Control: 100%
5. Specificity: This product will not cross react with positive samples of
human coronavirus HKU1 OC43, NL63 and 229E, SARS coronavirus, MERS
corona-virus, H1N1 (new influenza A (H1N1) virus (2009), the seasonal
H1N1) and H3N2, H5N1, H7N9, influenza B Yamagata, Victoria, respiratory
syncytial virus type A, B, parainfluenza type 1, 2, 3, rhinovirus species A, B
and C, adenovirus, 1, 2, 3, 4, 5, 7 and 55, enterovirus species A, B, C,
D,Human pulmonary virus, partial pulmonary virus, EB virus, measles virus,
human cytomegalo virus, rotavirus, norovirus, mumps virus, varicella-zoster
virus and mycoplasma pneumoniae, chlamydia pneumoniae, legionella,
bordetella pertussis, haemophilus influenzae, staphylococcus aureus,
streptococcus pneumoniae, pyogenic streptococcus, klebsiella pneumoniae,
mycobacterium tuberculosis, pyogenic streptococcus, candida albicans,
candida glabrata, newborn Cryptococcus and human genome DNA.
Description This kit utilizes fluorescence quantitative probe-based PCR and guarantees a high specificity to ensure accurate one-step identification of ORF1ab and N genes of 2019-nCoV. The kit offers a highly sensitive test with a limit of detection as low as 500 copies/ml. The results are available within 1.5 hour. Kit Contents (48 Tests /Kit) Dual Enzyme solution A 50 µl Dual Ncvo-O / N reaction solution A 1 ml Dual Ncvo-O / N positive control A 200 µl Dual Negative control A 200µl Applicable equipment ABI series, Bio-Rad, Roche series and other fluorescent real-time PCR instruments. Specimens requirements Specimen type: nasopharyngeal swab from suspected infection; virus cell culture fluid, etc. Basic Protocol 1. Sample preparation. Take the test sample and extract the RNA nucleic acid according to the instructions of the nucleic acid extraction kit. The nucleic acid extraction product should be stored at -20 C. 2. Reaction mixture preparation According to the total number of test samples, the number of PCR reaction tubes needed is. N = number of samples + 1 negative control + 1 positive control. The following protocol is recommended for a 20 µl reaction volume. If the volume of reaction changes, please adjust proportionally. Components Volume Dual Enzyme solution A 1 µl Dual Ncvo-O / N reaction solution A 19µl Final Volume: 20 µl 3. Sample adding. Add 5 µl each of the extracted RNA, positive and of negative control to corresponding reaction tubes. After assembling all the components, cover the tube caps and gently mix the contents of the tube, mix well, and centrifuge briefly. 4. Perform quantitative PCR Place the reaction tube inside a real-time PCR instrument. Set the channel and sample information, reaction system volume 25 µl. Select the following channels: FAM channel for nCOV-ORF1ab, VIC channel for nCOV-N. Perform quantitative PCR using recommended cycling parameters settings: Step Temperature Time Number of cycles Fluorescence Detector 1 50C 15 min 1 Off 2 95C 3 min 1 Off 3 95C 10sec 45 Off 60C 30 sec On Result analysis: 1) Set the baseline: Generally, it is set to 6-15cycle for ABI 7500, 7700 and other instruments, 3-15cycle for PE5700, and 6-12cycle for MJ Research Option2. Under special circumstances, the baseline can be adjusted accordantly. 2) Set the threshold: The threshold line just exceeds the highest point of the negative control amplification curve (random noise line).
ANDiS SARS-CoV-2 RT-qPCR Detection Kit ANDiS SARS-CoV-2 and Influenza A/B RT-qPCR Detection Kit ANDiS 350 Automated Nucleic Acid Extraction System
Temperature Block Temperature Range: 0 - 105 Max. Heating Rate: 7 /s Max. Cooling Rate: 5 /s
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RT PCR-Detection Kit
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Application Suspected case test in epidemic area Early test of fever clinic and CDC Screening of primary medical institutions Inspection of floating population in public areas Performance Characterisitics Sample tyep: nasal swab Time to result: 15-20 minutes Sensitivity: 97.70% Specificity: 99.84% Accuracy:98.97% Layperson study statistics 99.84% of non-professionals carried out the test without requiring assistance 99.82% of different types of results were interpreted correctly. Parameters: A Short Test Time: 15 mintues B Accuracy: More than 98.1% C Sensitivity: 96.4% Specificity: 100% D Easy operation: Nasal Swab E One Step solution F Lower cost with high efficiency G Package: 1 Test/Kit, 5 Tests/Kit, 25 Tests/Kit H It can detection for Omicron Virus Price and Minumum Quantity Price FOB in USD: 1.5Minimum Order Qty: 500000 Pieces Production Capacity: 100 Million Boxes Per WeekPackaging: 1,5,20,25,40,100,200 Pcs Per Box Sample: Available. Sample Cost Born By Buyer Product Model And Origin Brand: jwfModel: jwf01 Seller Code: Jwf Standards: Ce Competitive Advantages Lower cost with high efficiency Detection for omicron virus Easy operation Short test time Other Details Omicron covid test kit Covid test kit Omicron test card Covid test card
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