Buffer solution is a type of buffer system dedicated to life science research. In biochemical experiments, buffer solution plays an indispensable role. It can resist the influence of a small amount of strong acid and alkali from the outside, and maintain the system closest to the physiological environment. pH value. Both HEPES and PIPES buffers are commonly used buffers in biological experiments. The pH buffer range of HEPES is 6.8-8.2. It is a zwitterionic biological buffer. It is soluble in water and does not form stable complexes with metal ions. In most cases, it will not interfere with biochemical processes and can be widely used in a variety of Biochemical reactions and used as buffer reagents in certain cell culture media. HEPES is often used in the cell culture medium of various types of organisms; in protein research, PIPES is often used as the component and eluent of the binding buffer in cation exchange chromatography; in DNA research, PIPES is used as calcium phosphate and DNA The buffer for the precipitation formation system, AFM and the buffer for electroporation experiments. In addition, HEPES interferes with the reaction between DNA and restriction enzymes, and is not suitable for Lowry's method to determine protein content. HEPES buffer is often used in the research of organelles and highly variable, pH-sensitive proteins and enzymes, as well as in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. It is a hydrogen ion buffer that can control a constant pH range for a long time. The final concentration is 10-50mmol/L, and the buffering capacity can be achieved when the culture medium contains 20mmol/L HEPES. The pH buffer range of PIPES is 6.1-7.5, insoluble in water, soluble in NaOH aqueous solution. PIPES is different from buffers containing two (2-hydroxyethyl) amino groups (such as Bis-tris, Bicine), and can not form stable complexes with most metal ions. It is suitable for buffers in solution systems containing metal ions. According to existing research results, PIPES can be used to purify tubulin using phosphocellulose chromatography, to purify recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and to use as a buffer to crystallize transketolase from E. coli. In addition, because PIPES can form free radicals, it is not suitable for redox systems. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because PIPES has a relatively large ionic strength and its pKa value is concentration-dependent. We must learn to distinguish between the similarities and differences in these reagents, so as to better promote the development and production of our different products. Desheng unanimously adheres to this kind of subtle difference, so that it can continue to develop and produce better products.
Introduction: 4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells. Application: HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile. Buffer solution preparation: (1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L. (2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4�ºC or -20�ºC. Purpose: 1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA 2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase 3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer
Chemical, chemical raw material, auxiliaries, solvents, paint, coating, rubber, plastic, plasticizer, surfactant, additives, steel pipe, steel tube, stainless steel tube, stainless steel pipe, pipe fitting, flange, fasteners, steel wire, steel accessories.Transportation agent,customs clearance service,
Other Name: HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID INCI Name: HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID Molecular Formula: C8H18N2O4S Molecular Weight: 238.3 Appearance: White powder Purity: 98% CAS NO.: 7365-45-9 EINECS No.: 230-907-9 Application: cosmetics and skin care products Supplier: ZHENYIBIO HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID has a certain effect of softening cutin and assisting in whitening; in cosmetics, it is a hydrogen ion buffer, which can control a constant pH range for a long time. Hydroxyethylpiperazine ethane sulfonic acid is a very effective biological buffer, which can reform the stratum corneum and promote the metabolism of skin keratinocytes. It is an important mild exfoliating ingredient.
Can supply: PEX PIPES BALES SCRAP /WASTE. Post industrial. Can load in 1x40' HC container approx. 15 MT (10%) Ready for shipment. Photos available upon request. If you are interested kindly contact us for more information (price, available quantity etc.). You are welcome to visit our company premises and supervise our materials. Certificate ISO: 9001, ISO: 14001, OHSAS 45001, AQSIQ and CCIC.
Product Description: We supply connector in SS201, SS304, SS316, SS316L suitable for pipes OD42.2mm*2mm. Also available other OD on requist. Connectors in round, square any other custom designed shape. Finish: satin, Glossy.
We supply balustrade and its related components like connectors, saddles, pipes end caps in SS201, SS304, SS316, SS316L. Pipes OD42.2mm*2mm. Also available other OD on request. Balustrade shape round, square any other custom designed shape. Height about 1060mm. Finish: satin, Glossy.
GOOD TIME mini cookies in cups Available in various variants, sizes n packaging Original product by Arnot Indonesia
GOOD DAY coffee. Available in Ready to Drink bottles, instant pre mix in sachets For coffee powder, please refer to EXCELSO brand, KAPAL API brand and ABC brand
Good Day Coffe 600g Product Origin : Indonesia Key Specifications/Special Features : Food And Beverages, Instant Coffee, Coffee Minimum Order : 1x20'F
Product Name : Faucet Product Description : We supply faucets/ taps for washbasin, shower room, bathtubs, kitchen sink etc- brass, Stainless Steel, ABS with Chrome, Antique finish etc. Our faucets are tested for long use either with European make ceramic disc or Chinese make ceramic discs. Please contact us for details. Key Words : Faucets, Taps, shower Mixer, Bathtub mixer
General Purpose Fresh Coir Block (Processed & Compressed Coir) Coir pith blocks ensure high oxygen levels and therefore good drainage properties. They reduce the dormancy period of seeds and promote early sporting in almost all flowering plants and vegetable crops, We can supply coir blocks as Fresh Coir Blocks for vegetables/fruits and High Drain Rose Mix Coir Blocks specially designed to be used with Roses. Can be supplied in different weights, sizes or as per buyer’s requirements. Please contact us for more details and price quotations Advantages of Fresh Coir Blocks: Designed for vegetable and fruit plants Faster binding of soil Resistant to mold & rot; no chemical treatments needed Great air and water permeability Holds the seeds/saplings in place Excellent medium for quick vegetation No removal costs; Naturally degrades over a period of 2 to 5 years time Promotes deep rooting of plants Provides nutrients Easy to install Eco-friendly We can make 5 kg and 15 kg blocks.
Jumbo Bags : Name : Big bag/Bulk bag/Jumbo bag/FIBC/Super sack/Ton bag Raw Material : Polypropylene virgin material Color : White, blue, Beige, or whatever Printing : Onside or both sides in multi-colors,offset print color print Size : As the customers requirement Type of bag : U-panel,Circular,baffle Weave : 10x10,12x12,can be customized or as the customer requirement Denier : 700D to1000D Weights/m2 : 120gm to 240 gm or as the customers requirement Feature : Breathable and airy, anti-static, UV stabilization, reinforcement dust-proof, moisture- proof Sealing : Single/double fold saw bottom Top Filling : Top full open,top skirt,top filling spout Bottom : Flat bottom,discharge spout Saftey factory : From 3:1 to 6:1 Liner : coated or with liner bag for moisture proof Usage : sand,cement, chemical,mineral,sugar,food,flour,etc. Packing : 20pcs/bundle(bale), or as the customized MOQ : Above 1000pcs Delivery Time : 20-30 days after place an order Trade Term : FOB Payment Term : T/T
Raw Material : PP or BOPP Raw Material : Virgin material or recycled Color : All kinds of colors or as the customers requirement Printing : One side or both sides in multi-colors with film or non film Width : From 260-1300mm Length : As the customer’s requirement Weave : 10x10,11x11,12x12,14x14 or be customized Denier : 450D to 1500D Weights/m : 50g to 200g Surface Dealing : Anti-slip or plain Top : Heat cut or hemmed Bottom : Single folded, double folded, single stitch, double stitch Liner : coated or with liner bag for moisture proof Usage : rice, flour, sugar, salt, animal feed, asbestos, fertilizer, sand, cement and so on. Packing : 500pcs(bale),1000pcs(bale) or can be customized Minimum Order 20 feet container Delivery Time : 10-30days after place an order or negotiation Trade Term : CIF, CNF, FOB QINGDAO Special Requirements : As the customers requirements Treat : UV treated or as the customers requirement Loading qty. : 12 Tons/20’container, 23tons/40’hq. We can make all kinds of size
Raw Material: PP or BOPP Raw Material: Virgin material or recycled Color: All kinds of colors or as the customers' requirement Printing: One side or both sides in multi-colors with film or non film Width: From 260-1300mm Length: As the customer’s requirement Weave: 10x10,11x11,12x12,14x14,12×11or be customized Denier: 450D to 1500D Weights/m²: 50g to 200g Surface Dealing: Anti-slip or plain Top: Heat cut or hemmed Bottom: Single folded, double folded, single stitch, double stitch Liner: coated or with liner bag for moisture proof Usage: rice, flour, sugar, salt, animal feed, asbestos, fertilizer, sand, cement and so on. Packing: 500pcs(bale),1000pcs(bale) or can be customized Minimum Order20 feet container Delivery Time: 10-30days after place an order or negotiation Trade Term: CIF,CNF,FOB QINGDAO Special Requirements: As the customers' requirements Treat: UV treated or as the customers' requirement Loading qty.:12tons/20’container, 23tons/40’hq. we can make all kinds of size
1.We use imported base paper from Germany. For solid color, base paper most 80 to 120GSM. For wood base paper is between 70-85 gsm 2. High quality water-base ink- Available over 200 shades in Solid colors and wood grains 3. With clear texture ,high Fidelity meeting international standards. 4. With smooth surface coating 5. Germproof, mould proof, antistatic and wear-resistance. 6. Green environmental protection and Odorlessness which Conforms to the GB18584-2001 standard requirement 7. Can be used for the hot press molding craft, the production efficiency is high. 8. Melamine paper takes shape easily, is widely used for the panel furniture and the decorative yarn. Product Specification: Width: 1230-1260mm or 1300-1800mm Length: 500-1000m For storing: Put it in room to a cool and dry place keep the temperature 20-25°C in room. The best humidity is: 50-60% We can also give you complete decorative solution with Matching Melamine Edge Banding, PU Paper. If you need just need Melamine/prelaminated boards either in MDF / Particle board we can also do so
Base material:solid brass, ss304, ss316 Finish:chrome, satin nickel, pearl satin,pss-polished ss, sss- satin stainless steel, pvd, gold, brass polished For use on: tempered glass thickness 6mm to 12mm glass Edges:straight egdes/beveled edges Recommended glass door weight for three hinges:heavy duty hinges: 3 hinges- 54kgs Recommended glass door weight for two hinges: heavy duty hinges: 2 hinges- 34kgs Glass cuts: square glass cut, ear mouse glass cut, round glass cut Glass door swing:90,135, 180 degree, full swing.
LED light.
Pvc doors, doors with mdf molded door skins- with grains, white enamel painted, melamine faced, natural wood veneer, artificial veneer. Steel security doors. Flush doors Solid wooden doors with: oak, red cherry, sapelli, golden teak, merbau, walnut, rosewood, teak etc. Various panel designs. Door with frame. Moulds (casing).
Material: hdf Density:+950kg/m3 Door skin width: 660/740/840/920/1020mm Height: 2150mm Surface: natural wood veneer, melamine, enamel paint or plain door skin. We supply hdf moulded door skins, door panels, many designs to choose, with natural wood veneer, melamine finish, painted, with grains. Thickness 3.0 to 4.5mm, width of panel upto 1020. In natural wood veneer : sapele, mahogany, teak, golden teak, walnut. Wenge, black walnut, rosewood, oak, chinese oak, red white oak. Cherry, makore. Many of these veneers can be supplied with engineered veneered surface
