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African swine fever virus (ASFV) real-time PCR test kit enables the safe detection of African swine fever virus DNA in serum, blood, tissue, lymph nodes, spleen, muscle from pigs and breeding environment samples within 40 minutes. The kit consists of fluorescence PCR mix , positive control , negative control and lysis solution for real-time PCR amplification of ASFV. Fluorescence PCR mix contains primers, probes, enzyme and buffer for detection of the ASFV P72 gene. In addition, the role of lysis solution is to simply process samples and release DNA.
The Kit provides a rapid and easy method for the isolation of viral RNA and DNA from plasma, serum, and cellfree body fluids using silica membrane technology. Description The Kit provides a rapid and easy method for the isolation of viral RNA and DNA from plasma, serum, and cellfree body fluids. It is based on silica membrane technology and provides special buffer system. A specially formulated buffer system allows nucleic acid to bind to the membrane. Samples are lysed under denaturing conditions and then transferred to the nucleic acid column where nucleic acid binds and cellular debris, hemoglobin, and other proteins are washed away. High-quality nucleic acid is eluted in nuclease-free water. Phenol extraction and ethanol precipitation are not required. nucleic acid purified can be directly used in downstream molecular biology experiments such as PCR and real time qPCR, and next generation sequencing. Keys *Â The kit can extract viral RNA and DNA simply and fast by adopting the silica membrane spin column. * Safe and non-toxic because there's no need for phenol/chloroform extraction. * High-quality viral RNA and DNA can be obtained within 45 minutes. * Complete removal of contaminants and PCR inhibitors for downstream experiments. Features Specification Starting Amount - 200 ul Starting Material - Plasma, serum, and other acellular body fluids Elution Volume - 30-150 Technology - Column membrane technology Processing Mode - Manual Throughput - 1-24 samples/run Storage - Room temperature (15-25) Applications - PCR and real time qPCR, and next generation sequencing. Especially for Virus infectious diseases analysis.
The Kit follows a magnetic bead-based approach for the rapid and reliable isolation of viral RNA/DNA from nasopharyngeal (NP) swab specimens that are dry or in viral transport media (VTM). Description The Kit follows a magnetic bead-based approach for the rapid and reliable isolation of viral RNA/DNA from nasopharyngeal (NP) swab specimens that are dry or in viral transport media (VTM). The extraction methodology is easily adaptable to various automated systems and can also be scaled up or down depending on the amount of starting sample amount used. The kit enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. Nucleic acid isolated by the kit is stable with good integrity and purity, and can be directly used in downstream molecular biology experiments such as PCR and real time qPCR, and next generation sequencing. Keys *Â Magnetic bead-based purification *Â High-quality nucleic acid can be obtained range from 10 min to 40min (up to different protocol). * High throughput: Can be integrated with magnetic rod method automatic instrument or pipetting method automatic instrument to carry out high throughput extraction experiment. *Â Safe and nontoxic: No toxic organic reagents such as phenol/chloroform are needed. *Â Complete removal of contaminants and PCR inhibitors for downstream experiments. Features Specification Starting Amount - 200ul Starting Material - swab specimens or in viral transport media (VTM). Elution Volume - 80-150L Technology - Magnetic bead-based purification technology Processing Mode - Manual or Auto Throughput - 32/48/96/192 samples/run(up to different machine). Storage - Room temperature (15-25) Applications PCR and real time qPCR, and next generation sequencing.
The disposable virus sampling tube/Virus Transport Medium(VTM) is suitable for nucleic acid extraction of SARS-CoV-2 virus, various influenza viruses, avian influenza viruses, rubella, swine fever and other virus samples. The disposable virus sampling tube/Virus Transport Medium(VTM) is suitable for nucleic acid extraction of SARS-CoV-2 virus, various influenza viruses, avian influenza viruses, rubella, swine fever and other virus samples. It is also suitable for the collection and transportation of specimens of Chlamydia, Mycoplasma, and Mycoplasma Arthroplasma. Samples are usually collected from the mouth, throat, nasal cavity, and anus. Sampling tube: The tube body and tube cover are made of polypropylene, which will not deform at high temperature and high pressure (121C, 15min), and will not become brittle at low temperature (196C). Resistant to static extrusion and dynamic impact. The bottom tapered design is resistant to centrifugation and vibration. Built-in sample preservation solution is used for sample preservation to prevent leakage. Sample preservation solution: Functioned with virus lysis solution and virus nucleic acid preservation solution. The virus lysate realizes the inactivation of the virus in the sample and reduces the risk of secondary infection. The unique protection system can prevent nucleic acid from being degraded and improve the accuracy of downstream detection. The sample has a wide adaptability and can be used for the collection, preservation and transportation of various swabs. Sampling swabs: sampling swabs are flocking sampling heads, which are non-toxic to cells and viruses, and do not contain RNase, which can greatly increase the collection and release of virus samples , ensure the accuracy of PCR detection results. The plastic rod is made of ABS, with a unique molded breaking point design, and there is no tiny debris during the breaking process. Features: 1.More collection and release:nylon flocked swabs provide optimum specimen collection because of their ability to collect more specimen at the collection site and to more rapidly release the entire samples specime 2. High safety factor: it can lyse pathogens, inactivate viruses, and reduce the risk of secondary infection. 3. Stable nucleic acid: a unique protection system that can prevent nucleic acid from being degraded and improve the accuracy of downstream detection. 4. Wide sample adaptability: it can be used for the collection, preservation and transportation of various swabs,Including oral swabs, throat swabs, nasal swabs, vaginal swabs and so on.
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