African swine fever virus (ASFV) real-time PCR test kit enables the safe detection of African swine fever virus DNA in serum, blood, tissue, lymph nodes, spleen, muscle from pigs and breeding environment samples within 40 minutes. The kit consists of fluorescence PCR mix , positive control , negative control and lysis solution for real-time PCR amplification of ASFV. Fluorescence PCR mix contains primers, probes, enzyme and buffer for detection of the ASFV P72 gene. In addition, the role of lysis solution is to simply process samples and release DNA.