The Kit provides a rapid and easy method for the isolation of viral RNA and DNA from plasma, serum, and cellfree body fluids using silica membrane technology.
Description
The Kit provides a rapid and easy method for the isolation of viral RNA and DNA from plasma, serum, and cellfree body fluids. It is based on silica membrane technology and provides special buffer system. A specially formulated buffer system allows nucleic acid to bind to the membrane. Samples are lysed under denaturing conditions and then transferred to the nucleic acid column where nucleic acid binds and cellular debris, hemoglobin, and other proteins are washed away. High-quality nucleic acid is eluted in nuclease-free water. Phenol extraction and ethanol precipitation are not required. nucleic acid purified can be directly used in downstream molecular biology experiments such as PCR and real time qPCR, and next generation sequencing.
Keys
*Â The kit can extract viral RNA and DNA simply and fast by adopting the silica membrane spin column.
* Safe and non-toxic because there's no need for phenol/chloroform extraction.
* High-quality viral RNA and DNA can be obtained within 45 minutes.
* Complete removal of contaminants and PCR inhibitors for downstream experiments.
Features
Specification
Starting Amount - 200 ul
Starting Material - Plasma, serum, and other acellular body fluids
Elution Volume - 30-150
Technology - Column membrane technology
Processing Mode - Manual
Throughput - 1-24 samples/run
Storage - Room temperature (15-25)
Applications - PCR and real time qPCR, and next generation sequencing.
Especially for Virus infectious diseases analysis.
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Virus DNA/RNA Extraction Kit(Magnetic Beads Based) Simple and fast : extraction within 20min, no centrifuge and can be used for automated extraction Safer : quickly inactivate viruses during extraction Wide used : extracting highly pure viral nucleic acid (DNA/RNA) from samples such as human nasopharyngeal swabs, sputum, broncho lavage fluid and alveolar lavage fluid High yield: high nucleic acid extraction efficiency, making downstream detection more sensitive High purity : Maximum removal of inhibitory impurities, high purity nucleic acid obtained can be directly used for downstream detection Production Description This product is used for the steps of nucleic acid extraction, enrichment and purification. This kit is applicable for extracting highly pure viral nucleic acid (DNA/RNA) from samples such as human nasopharyngeal swabs, sputum, broncho lavage fluid and alveolar lavage fluid. The obtained nucleic acid can be used in the clinical in vitro testing. HIGH YIELD Using magnetic beads method virus DNA / RNA extraction kit (Vazyme # RM102) and another virus extraction kit (from TI company) to extract nucleic acids from four samples, namely SARS-CoV-2 pseudovirus, African swine fever virus 3(ASFV), Porcine Epidemic Diarrhea Virus (PEDV) and HEK293 cells, and detected by qPCR detection. From the test results, it can be seen that The Ct value of the RNA extracted by the virus DNA / RNA extraction kit (Vazyme # RM102) is 1-3 lower, indicating the efficiency of Vazyme # RM102 is higher than other brands of similar virus extraction kits. Wide compatibility Using the virus DNA / RNA extraction kit (Vazyme # RM102) to extract the SARS-CoV-2 pseudovirus with an initial amount of 10-100000 copies, , then detected by the new coronavirus (SARS-CoV-2) nucleic acid detection kit. The test results show that the extraction kit is wide compatibility. Storage 15 ~ 25�°C, It is recommended to store the magnetic beads at 2 ~ 8�°C. Please be noted the beads shall not be stored below 0�°C.
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