T7 High Yield RNA Synthesis Kit optimizes the transcription reaction system. The kit can synthesize the single-stranded RNA efficiently by uses T7 RNA polymerase, the linear double-stranded DNA with the T7 promoter sequence as the template, NTPs as the substrate to control the DNA sequence downstream of the promoter. During transcription, modified nucleotides can be added to the substrate to prepare biotin or dye-labeled RNA.
This kit can synthesize long transcripts and short transcripts., RNA can be produced 100-200 g with 1 g of template input. The RNA synthesized by transcription can be used for such as RNA structure and function research, RNase protection, probe hybridization, Various downstream applications such as RNA, microinjection and in vitro translation.
Multiplex One Step RT-qPCR Probe Kit is a multiplex quantitative PCR kit based on RNA as template. In the process of the experiment, reverse transcription and quantitative PCR were carried out in the same tube, which simplified the experimental operation and reduced the risk of contamination.
In this kit, the first strand cDNA was efficiently synthesized by heat-resistant Hifair V reverse transcriptase and quantitatively amplified by UNICON HotStart Taq DNA polymerase. The kit mainly contains optimized MP buffer, enzymes mix, etc. The buffer solution already contains Mg2+ and dNTP. In addition, the factors that can effectively inhibit the non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reactions are added, which can ensure the amplification efficiency and carry out up to four reactions.
Quantity: 100 T, 1000 T, 10000 T
Applications: RT-qPCR, 4-plex reaction
Sample Type: RNA template
main enzyme: Hifair V Reverse Transcriptase,UNICON HotStart Taq DNA Polymerase
Category: IVD PCR
Research Area: RT-qPCR
Direct Taq DNA Polymerase is a hot-start DNA polymerase that is resistant to blood and other inhibitors. This product is blocked with antibodies and has good amplification sensitivity and specificity. This product can be completely inactivated by heating the blocking antibody for 30 sec at the pre-denaturation temperature, releasing the DNA polymerase activity. The use of the hot-start Taq enzyme can effectively inhibit the amplification caused by the non-specific annealing of primers.
Quantity: 250U, 500U, 1000U
Enzyme Family: DNA Polymerase
Enzyme Subfamily: Taq Polymerase
Application: Hot Start PCR
Enzyme Feature: Hot Start
