T7 High Yield RNA Synthesis Kit optimizes the transcription reaction system. The kit can synthesize the single-stranded RNA efficiently by uses T7 RNA polymerase, the linear double-stranded DNA with the T7 promoter sequence as the template, NTPs as the substrate to control the DNA sequence downstream of the promoter. During transcription, modified nucleotides can be added to the substrate to prepare biotin or dye-labeled RNA. This kit can synthesize long transcripts and short transcripts., RNA can be produced 100-200 g with 1 g of template input. The RNA synthesized by transcription can be used for such as RNA structure and function research, RNase protection, probe hybridization, Various downstream applications such as RNA, microinjection and in vitro translation.