Trustwell Chik Igm Elisa Kit Suppliers and Manufacturers

Product Details: Number of Reactions(Preps)/Kit : 96 Wells Brand : CTK BioTech Sample : Serum / Plasma Storage : 2-8 C Cat No : AE0315 The TRUSTwell CHIK IgM Test is a solid phase enzyme linked immunoabsorbent assay for the qualitative detection of IgM anti- Chikungunya virus in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of acute infection with Chikungunya viruses. Any reactive specimen with the TRUSTwell CHIK IgM Test must be confirmed with alternative testing method(s) and clinical findings. TEST PRINCIPLE: TRUSTwell CHIK IgM Test is a solid phase enzyme linked immunoabsorbent assay based on the principle of the indirect immunoassay technique for the detection of IgM anti- Chikungunya virus in human serum or plasma. The TRUSTwell CHIK IgM Test is composed of two key components: 1) Solid microwells pre-coated with recombinant CHIK antigen(s); 2) Liquid conjugates composed of monoclonal anti-human IgM reagent conjugated with horse reddish peroxidase (HRP-anti-Human IgM conjugates). During the assay, the test specimen is first incubated with the coated microwells. IgM anti- Chikungunya virus, if present in the specimen, binds to the antigen coated on the microwell surface. In the second incubation with the HRP-anti Human IgM conjugates, the IgM antiChikungunya antibody absorbed on the surface of microwell reacts to the conjugates, forming a complexed conjugates. Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbances are read using a spectrophotometer at 450 /620-690 nm. Clinical Performance: A total of 336 specimens from susceptible subjects were tested by TRUSTwell CHIK IgM and by a commercial reference ELISA kits. Relative Sensitivity: 91.3 %, (95 % CI: 73.2 % -97.6 %) Relative Specificity: 96.8 %, (95 % CI: 94.2 % -98.3 %) Overall Agreement: 96.4%, (95 % CI: 93.9 % -98.0 %).

Product Details: Number of Reactions(Preps)/Kit : 96 Wells Bran : CTK BioTech Sample Material : serum or plasma Storage : 2-8 C Cat. No : AE0310 The Dengue IgM ELISA Kit is a solid-phase enzyme-linked immunosorbent assay for the qualitative detection of IgM anti-dengue viruses (DEN1, 2, 3, 4) in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of acute infection with dengue viruses. TEST PRINCIPLE: The Dengue IgM ELISA Kit is a solid-phase enzyme-linked immunosorbent assay based on the principle of the capture immunoassay methodology for the detection of IgM anti-dengue virus in human serum or plasma. The Dengue IgM ELISA Kit is composed of two key components: 1) Solid microwells pre-coated with mouse monoclonal anti-human IgM antibody. 2) Conjugate working solution composed of dengue antigen and HRP-anti-dengue conjugates. During the assay, the test specimen is first incubated in the coated microwell. IgM antidengue, if present in the specimen, binds to the anti-human IgM antibodies coated on the microwell surface, and any unbound specimen is then removed by a wash step. During a second incubation with HRP-anti-dengue conjugate working solution, the IgM anti-dengue absorbed on the surface of microwell binds to the conjugate through dengue antigen, forming a conjugate complex. Unbound conjugates are then removed by washing. After addition of the TMB substrate, the presence of the conjugate complex is shown by development of a blue color resulting from a reaction between the enzyme and substrate. This reaction is then quenched by addition of the Stop Solution, and the absorbance value for each microwell is determined using a spectrophotometer at 450/620-690 nm. Accuracy of Detection : A total of 491 patient specimens were collected from susceptible subjects and tested by Dengue IgM ELISA Kit and by a commercial leading brand EIA. Relative Sensitivity: 90.8% (95% Confidence Interval = 85.5 - 94.4%) Relative Specificity: 95.9% (93.1 - 97.7%) Overall Agreement: 94.1% (91.6 - 95.9%).

Product Details: Number of Reactions(Preps)/Kit : 96 Wells Brand : CTK BioTech Sample : serum / plasma Storage : 2 - 8 C Cat No : AE0710 INTENDED USE: The TRUSTwell HBsAg ELISA Kit is a solid phase enzyme linked immunosorbent assay for the qualitative detection of hepatitis B virus surface antigen (HBsAg) in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of infection with HBV. Any reactive specimen with the TRUSTwell HBsAg ELISA Kit must be confirmed with alternative testing method(s) and clinical findings. TEST PRINCIPLE: TRUSTwell HBsAg ELISA Kit is a solid phase enzyme linked immunosorbent assay based on the principle of antibody sandwich technique for the detection of HBsAg in human serum or plasma. The TRUSTwell HBsAg ELISA Kit is composed of two key components: 1) Solid microwells pre-coated with monoclonal anti HBsAg antibody; 2) Liquid conjugates composed of polyclonal anti-HBsAg conjugated with horse radish peroxidase (HRP-HBsAb conjugates). During the assay, the test specimen and HRP-HBsAb conjugates are incubated simultaneously with the coated microwells. HBsAg, if present in the specimen, reacts to the anti-HBsAg antibody coated on the microwell surface as well as the HRPHBsAb conjugate, forming sandwich complex conjugates. Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbance are read using a spectrophotometer at 450 /620-690 nm. Clinical Performance: A total of 1033 patient specimens from susceptible subjects were tested by the TRUSTwell HBsAg ELISA Kit. Relative Sensitivity:100%, Relative Specificity:99.78%, Overall Agreement:99.81%

Product Details: Number of Reactions(Preps)/Kit : 96 Wells Brand : CTK BioTech Sample : serum or plasma Storage : 2-8 c Cat No : AE0311 INTENDED USE : The Dengue IgG ELISA is a solid-phase enzyme-linked immunosorbent assay for the qualitative detection of elevated anti-dengue viruses (DEN1, 2, 3, 4) IgG in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of active infection with dengue viruses, including secondary infection. TEST PRINCIPLE: The Dengue IgG ELISA Kit is a solid-phase enzyme-linked immunosorbent assay based on the principle of the capture immunoassay methodology for the detection of IgG anti-dengue virus in human serum or plasma. The Dengue IgG ELISA Kit is composed of two key components: 1) Solid microwells pre-coated with mouse monoclonal anti-human IgG antibody. 2) Conjugate composed of biotinylated dengue antigens and avidin HRP-conjugates. During the assay, the test specimen is first incubated in the coated microwell. IgG antidengue, if present in the specimen, binds to the anti-human IgG antibodies coated on the microwell surface, and any unbound specimen is then removed by a wash step. During a second incubation with Biotinylated dengue antigens, the IgG anti-dengue absorbed on the surface of microwell binds with dengue antigen, forming a complex. Unbound antigens are then removed by washing. After addition of Streptavidin HRP conjugate, it binds with biotinylated antigen and forming complex with HRP conjugate. After addition of the TMB substrate, the presence of the conjugate complex is shown by development of a blue color resulting from a reaction between the enzyme and substrate. This reaction is then quenched by addition of the Stop Solution, and the absorbance value for each microwell is determined using a spectrophotometer at 450/620-690 nm. Accuracy of Detection : A total of 262 patient specimens were collected from susceptible subjects and tested by Dengue IgG ELISA Kit and by a commercial leading brand EIA. Relative Sensitivity: 95.2% (95% Confidence Interval = 83.8 - 99.4%) Relative Specificity: 96.8% (95% Confidence Interval = 93.6 - 98.7%) Overall agreement: 96.6% (95% Confidence Interval = 93.6 - 98.4%)

Product Details: Number of Reactions(Preps)/Kit 96 Wells Brand CTK BioTech Sample serum or plasma Storage 2 - 8 C Cat No : AE0510 INTENDED USE: The TRUSTwell HCV IgG ELISA Kit is a solid phase enzyme linked immunosorbent assay for the qualitative detection of IgG to Hepatitis C virus (HCV) in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of infection with HCV. Any reactive specimen with the TRUSTwell HCV IgG ELISA Kit must be confirmed with alternative testing method(s) and clinical findings. TEST PRINCIPLE: The TRUSTwell HCV IgG ELISA Kit is a solid phase enzyme linked immunosorbent assay based on the principle of the indirect EIA technique for the detection of the IgG to HCV in human serum or plasma. The TRUSTwell HCV IgG ELISA Kit is composed of two key components: 1) Solid microwells pre-coated with recombinant HCV antigens; 2) Liquid conjugates composed of mouse anti-human IgG conjugated with horse radish peroxidase (HRP-anti Human IgG conjugates). During the assay, the test specimen is first incubated with the coated microwells. The IgG anti HCV, if present in the specimen, bind to the antigens coated on the microwell surface. In the second incubation with the HRP-anti-human IgG conjugates, the IgG antibodies absorbed on the surface of microwell react to the HRP-anti-human IgG conjugates. Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbances are read using a spectrophotometer at 450 /620-690 nm. Clinical Performance: A total of 1108 patient specimens from susceptible subjects were tested by the TRUSTwell HCV IgG ELISA Kit. Relative Sensitivity:100%, Relative Specificity:99.81%, Overall Agreement:99.82%.

Product Details: Number of Reactions(Preps)/Kit : 96 Wells Brand : CTK BioTech Sample : serum / plasma Storage : 2-8 C Cat No : AE0420 INTENDED USE: The TRUSTwell HIV Ag-Ab ELISA Kit is a solid phase enzyme linked immunosorbent assay for the qualitative detection of HIV-1 P24 antigen, anti-HIV-1 including subtype O and anti-HIV-2 antibodies (including isotype IgG, IgM and IgA) in human serum or plasma. It is intended for professional use only as an aid in the early identification of infection with HIV-1 and HIV-2 viruses. Any reactive specimen with the TRUSTwell HIV Ag-Ab ELISA Kit must be confirmed with alternative testing method(s) and clinical findings. TEST PRINCIPLE: The TRUSTwell HIV Ag-Ab ELISA Kit is a solid phase enzyme linked immunosorbent assay based on the principle of the double antibody/antigen sandwich technique for the detection of HIV-1 P24 antigen and various antibodies against HIV-1 and/or HIV-2 antigen in human serum or plasma. The TRUSTwell HIV Ag-Ab ELISA Kit is composed by two systems: 1) Solid microwells pre-coated with Recombinant HIV-1 and HIV-2 antigens and anti-P24 antibody 2) Liquid conjugates composed of a. Recombinant HIV-1 and HIV-2 antigens conjugated with horse radish peroxidase (HRP-HIV 1+2 conjugates) and avidin conjugated with HRP (HRP-avidin conjugates) b. Anti-P24 antibody conjugated with biotin (Biotinylated P24 Ab) During the assay, the test specimen is first incubated with the coated microwells and followed by incubation with biotinylated P24 Ab. The anti-HIV-1 and anti-HIV-2 antibodies, if present in the specimen, bind to the antigens coated on the microwell surface. If P24 present in the specimen, binds to the anti-P24 antibody on the microwell surface and biotinylated P24 Ab in the solution. In the next incubation with the HRP-HIV 1+2 and HRP-avidin conjugates, the above bounded anti-HIV-1 and anti-HIV-2 antibodies and bounded P24 antigen reactive to the HRP conjugates, forming HRP-complex. Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbance are read using a spectrophotometer at 450/620-690nm. Clinical Performance: A total of 1100 patient specimens from susceptible subjects were tested by the TRUSTwell HIV Ag-Ab ELISA Kit. Relative Sensitivity: 100%, Relative Specificity: 99.9%, Overall Agreement: 99.9%.

Product Details: Number of Reactions(Preps)/Kit : 96 Wells Brand : CTK BioTech Sample : serum / plasma Stroage : 2-8 C Cat No : AE0410 INTENDED USE: The TRUSTwell HIV 1+2 Ab ELISA Kit is a solid phase enzyme linked immunosorbent assay for the qualitative detection of anti-HIV-1 including subtype O and anti-HIV-2 antibodies (including isotype IgG, IgM and IgA) in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of infection with HIV-1 and HIV-2 viruses. Any reactive specimen with the TRUSTwell HIV 1+2 Ab ELISA Kit must be confirmed with alternative testing method(s) and clinical findings. TEST PRINCIPLE : TRUSTwell HIV 1+2 Ab ELISA Kit is a solid phase enzyme linked immunosorbent assay based on the principle of the double antigen-sandwich technique for the detection of the various antibodies against HIV-1 and/or HIV-2 in human serum or plasma. The TRUSTwell HIV 1+2 Ab ELISA Kit is composed of two key components: 1) Solid microwells pre-coated with recombinant HIV-1 and HIV-2 antigens; 2) Liquid conjugates composed of recombinant HIV-1 and HIV-2 antigens conjugated with horse radish peroxidase (HIV 1+2 HRP conjugates). During the assay, the test specimen is first incubated with the coated microwells. The anti-HIV-1 and anti-HIV-2 antibodies, if present in the specimen, bind to the antigens coated on the microwell surface. In the second incubation with the HRP- HIV 1+2 conjugates, the anti-HIV-1 and antiHIV-1 antibodies absorbed on the surface of microwell react to the HRP-HIV 1+2 conjugates. Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbance are read using a spectrophotometer at 450 /620-690 nm. Clinical Performance : A total of 1095 patient specimens from susceptible subjects were tested by the TRUSTwell HIV 1+2 Ab ELISA Kit. Relative Sensitivity: 100% Relative Specificity: 99.9%, Overall Agreement: 99.9%

Product Details: Number of Reactions(Preps)/Kit : 96 Wells Brand : InBios Sample Material : Serum Format : Immunocapture Incubation Time : 30+30+30+10 Cat No : CHKM-R The CHIKjj Detect IgM ELISA is designed for the qualitative detection of IgM antibodies present in human serum targeting Chikungunya virus E2/E1 proteins. This test is not intended to screen blood or blood components. This test is for research use only. Not for use in diagnostic procedures. The complete kit includes one 96 well strippable plate with all necessary reagents and controls. Key Features : InBios chikungunya kits have been developed using a novel technology1 that outperforms traditional antigen preparations, resulting in highly sensitive detection of recent and past CHIKV infection. Excellent results in CDC evaluation of commercially available chikungunya IgM assays. Ordering: Cat No CHKM-R Formet Immunocapture Quantity 96 wells/Plate Incubation Time 30 + 30 + 5 + 10 Sample Type Serum Storage 2-8 Shelf Life 12 months

Product Details: Brand : CTK BIOTECH Size : 96 Well Specimen : Serum & Plasma stored at 2 - 8 C Cat No : E0100 The RecombiLISA HAV IgM ELISA is a solid-phase enzyme-linked immunosorbent assay for the qualitative detection of IgM anti-hepatitis A virus in human serum or plasma. Product Features: Utilizes IgM capture technique Detects IgM anti-HAV in serum or plasma Evaluated with BBI HAV serum conversion and HAV mixed titer performance panels Useful for identifying acute HAV infections Product Specification: Microwells coated with anti-human IgM antibody HAV IgM negative control HAV IgM positive control HRP-HAV conjugates Wash buffer (30 x concentrate) TMB substrate A TMB substrate B Stop solution ELISA Working Sheet Product insert

Product Details: Number of Reactions(Preps)/Kit : 96 Wells Brand : CTK BioTech Sample Material : Plasma/Serum Storage : 2-8 C Cat. No : E0105 INTENDED USE: The RecombiLISA HEV IgM ELISA is a solid-phase enzyme-linked immunosorbent assay for the qualitative detection of anti-hepatitis E virus (HEV) IgM in human serum or plasma. It is intended for professional use only and to be used as an aid in the diagnosis of infection with HEV. TEST PRINCIPLE: The RecombiLISA HEV IgM ELISA is a solid-phase enzyme-linked immunosorbent assay based on the principle of the IgM capture technique for the detection of antiHEV IgM in human serum or plasma. The RecombiLISA HEV IgM ELISA is composed of two key components: 1) Solid microwells pre-coated with polyclonal anti-human IgM antibody 2) Liquid conjugate composed of HEV antigen conjugated with horseradish peroxidase (HRP-HEV Conjugate) During the assay, the test specimen is first incubated in the coated microwells. AntiHEV IgM, if present in the specimen, binds to the antibody coated on the microwell surface. Any unbound specimen is then removed by a wash step. During a second incubation with the HRP-HEV Conjugate, the anti-HEV IgM antibody is absorbed on the microwell surface which binds to the HRP-HEV Conjugate, forming a conjugate complex. Unbound conjugate is then removed by washing. After addition of the TMB substrate, the presence of conjugate complex is shown by development of a blue color resulting from a reaction between the enzyme and substrate. The reaction is then quenched by addition of the Stop Solution, and the absorbance value for each microwell is determined using a spectrophotometer at 450/620-690 nm. Clinical Performance: A total of 400 specimens from susceptible subjects were tested by the RecombiLISA HEV IgM ELISA and by a Chinese State Drug Administration (SFDA) licensed reference EIA. Clinical Performance: Relative Sensitivity: 100%, Relative Specificity: 100%, Overall Agreement: 100%.

Product Details: Number of Reactions(Preps)/Kit : 96 Wells Brand : CTK BioTech Sample : serum or plasma Storage : 2-8 C Cat No : E0330 The RecombiLISA Leptospira IgM ELISA Kit is an indirect, solid phase enzyme-linked immunosorbent assay. The two main kit components are: 1) A 96 breakable well plate with the solid microwells pre-coated with L. interrogans antigen. 2) Liquid conjugates composed of monoclonal anti-human IgM reagent conjugated with horseradish peroxidase (HRP-anti-human IgM conjugates). This test is intended to be used for the qualitative detection of IgM anti-Leptospira interrogans (L. interrogans) in human serum or plasma. It is intended to be used by professionals as an aid in the diagnosis of acute infection with L. interrogans. Any interpretation or use of this test result must also rely on other clinical findings as well as on the professional judgment of health care providers. Recommended for use: Any person presenting with clinical signs or symptoms consistent with acute infection with interrogansincluding: high fever, headache, chills, muscle aches, vomiting, jaundice, red eyes, abdominal pain, diarrhea, skin rash After exposure to a known contaminated source Product Features : Sensitivity: 86.7% Specificity: 96.8% Cross Reactivity: No false positive results were observed on specimens from Dengue, Malaria, Typhi, ANA, HAMA, RF, HIV, HBsAg, HCV, Syphilis, pylori Interference: No interference was seen with substances: Salicylic acid: 4.34 mmol/L; Glucose: 55 mmol/L; Sodium citrate: 1.3 %;Heparin: 3,000 U/L; Creatinine: 5 mmol/L; Bilirubin: 10 mg/dL; EDTA: 3.4 �µmol/L Shelf life: 12 months Open vial stability: 8 weeks at 2-8 C, or until the expiration date Kit Components : Microwells coated with recombinant L. interrogans antigens Lepto IgM positive control Lepto IgM negative control Sample diluent HRP anti-human IgM conjugate Wash buffer (30 x concentrate) TMB substrate A TMB substrate B Stop solution ELISA Working Sheet Product insert

Product Details: Number of Reactions(Preps)/Kit : 96 Wells Brand : CTK BioTech Sample : serum or plasma Storage : 2 - 8 C Cat No : AE0610 INTENDED USE: The TRUSTwell Syphilis Ab kit is a solid phase enzyme linked immunosorbent assay for the qualitative detection of antibodies (IgG, IgM, IgA) against Treponema pallidum (Tp) in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of infection with Tp. Any reactive specimen with the TRUSTwell Syphilis Ab ELISA Test must be confirmed with alternative testing method(s) and clinical findings. TEST PRINCIPLE: The TRUSTwell Syphilis Ab ELISA Test is a solid phase enzyme linked immunosorbent assay based on the principle of the double antigen sandwich technique for the detection of antibodies to Tp in human serum or plasma. The TRUSTwell Syphilis Ab ELISA Test is composed of two key components: 1) Solid microwells pre-coated with recombinant Tp antigens; 2) Liquid conjugates composed of recombinant Tp antigens conjugated with horseradish peroxidase (HRP-Tp conjugates). During the assay, the test specimen and HRP-Tp conjugates are incubated simultaneously with the coated microwells. Antibodies (IgG, IgM, or IgA) to Tp if present in the specimen, reacts to the Tp antigens coated on the microwell surface as well as the HRP-Tp conjugates, forming sandwich complex conjugates. Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbances are read using a spectrophotometer at 450 /620-690 nm. Clinical Performance: A total of 1132 patient specimens from susceptible subjects were tested by the TRUSTwell Syphilis Ab ELISA Kit. Relative Sensitivity:100%, Relative Specificity:99.8%, Overall Agreement:99.82%.

Product Details: Number of Reactions(Preps)/Kit ; 96 wells Brand : Abbott Test Type : Elisa CAT No : E-LEP01M / E-LEP01M05 The Panbio Leptospira IgM ELISA is for the qualitative detection of IgM antibodies to leptospira in serum as an aid in the clinical laboratory diagnosis of patients with clinical symptoms consistent with leptospirosis.

Elisa kit Available in Various Parameters mainly HIV1, 2, HCV, HBs Ag In 36,48,96 tests packing

Product Details: Number of Reactions(Preps)/Kit : 96 wells Brand : Abbott Usage/Application : Hospital Sample Material : Serum CAT No : 11EK10 The Panbio Dengue IgM Capture ELISA is used to detect IgM antibodies to dengue antigen in serum as an aid to clinical laboratory diagnosis of patients with clinical symptoms consistent with dengue fever. The Panbio. Dengue IgM Capture ELISA should be used in conjunction with other dengue serology.

Product Details: Brand : Abbott Package type : Microplate Pack size : 96wells/Kit Specimen : Serum Description : Dengue IgM Capture ELISA Cat.No. : 11EK20 Bioline DENGUE IgM CAPTURE ELISA In primary infection with the dengue virus, IgM antibody becomes detectable about five days after disease onset, when circulating virus declines in the blood. IgM level rises quickly to peak at about 2 weeks and declines to undetectable levels after 2-3 months In secondary infections, IgM response is typically at a lower level compared to that in a primary infection. Early diagnosis of dengue infection (especially in primary dengue infection) High accuracy with all dengue serotypes (DEN1,2,3, and 4) Simple and easy to use: All necessary reagents included in the kit Shelf life and storage temperature: 18 months from the date of manufacturing at 2-8 C Included in the WHO Bulk Procurement Scheme Performance: Sensitivity 96.4 %, Specificity 98.9 % (vs. HAI test).

The Toxoplasma gondii IgM GENLISA ELISA is intended for the qualitative determination of Toxoplasma gondii IgM antibody in human serum and plasma. Pack Size - ELISA 96 wells