Stem Cell Liquid Nitrogen Tank Kgsq Suppliers and Manufacturers

In biological sample centers or scientific research institutions, there are a large number of cells, strains, vaccines and other samples that need to be cryopreserved. The scientific research of these samples is of great significance, and their safety cannot be ignored. In addition, there are requirements for batch management and data collection. Vapor phase liquid nitrogen tanks are often chosen over aviation aluminum Dewars. Vapor-phase liquid nitrogen tank, also known as "stem cell liquid nitrogen tank", is named after it is often used to establish stem cell banks. It is different from ordinary liquid nitrogen containers. It has two storage modes: gas phase and liquid phase. The sample storage method is unconventional and its working principle is break the usual. Sample storage methods in gas phase liquid nitrogen tanks: Partition management, gas phase preservation! The inner tank of the stem cell liquid nitrogen tank is divided into two areas. The upper area is for samples, which is the storage area for stem cells, embryos, bacteria, etc.; the lower area is for liquid nitrogen storage only and does not contain any samples. The bottom position, which is the liquid nitrogen storage area, is equipped with an evaporator to provide evaporated nitrogen for sample storage to cool down. The sample is only "close" to the liquid nitrogen, but does not touch it, and the volatilized low-temperature nitrogen is used to store the cells. Working principle of gas phase liquid nitrogen tank: The principle is actually very simple, such as the use of liquid nitrogen evaporation cooling mentioned above! When the nitrogen in the sample storage area is insufficient to support the sample demand, the bottom evaporator starts working to supply liquid nitrogen to the upper part. This has the advantage of avoiding sample cross-contamination. The reason why I donâ??t mention its other storage mode - liquid phase is because the liquid nitrogen reserve in the container is large, much higher than that of conventional liquid nitrogen containers, and the evaporation loss of stainless steel is higher than that of aluminum alloy. When storing in liquid phase, replenish the liquid. Frequent and troublesome, difficult to use, only gas phase storage is recommended. In addition, the gas-phase liquid nitrogen tank also has high dual-gallery vacuum + high thermal insulation performance, which reduces heat conduction, reduces the evaporation rate of liquid nitrogen, and maintains stable pressure inside the tank. The built-in intelligent control management system is used for real-time detection and abnormal warning of temperature, pressure and liquid level to ensure the stability of the tank and the safety of stem cells. If you have any needs, please contact KGSQ liquid nitrogen tank:

As we all know, the storage modes of liquid nitrogen tanks can be divided into two types according to sample storage requirements: gas phase and liquid phase, that is, soaking in liquid nitrogen and storing in nitrogen. In order to make it easier for users to distinguish between the two, regardless of industry or brand, they are accustomed to using storage modes to identify them. Liquid phase storage is liquid phase liquid nitrogen tanks, and gas phase storage is gas phase liquid nitrogen tanks. This is also their main way of distinguishing themselves! Of course, there are other differences besides this, letâ??s introduce them one by one. 1. Model differences: Including capacity size and national standard naming. The liquid phase liquid nitrogen tank has a capacity range of 1-175 liters and is named YDS. The national standard stipulates that the material is aluminum alloy. The gas phase liquid nitrogen tank has a capacity range of 350-1800 liters and is named YDD. The material specified by the national standard is stainless steel. For user use, just select the appropriate model and type based on the number of samples. 2. Mode differences: Liquid phase liquid nitrogen tank, the sample is immersed in liquid nitrogen; The gas phase liquid nitrogen tank meets both liquid phase and gas phase modes. There is a liquid nitrogen storage area at the bottom of the tank to provide liquid nitrogen to the sample area. Note: Vapor phase storage also requires supplementing liquid nitrogen. 3. Management differences: Liquid-phase liquid nitrogen tanks have a wide range of uses and are relatively small in size. Sample classification management relies on buckets and freezing racks. There is no intelligent control and requires manual labor. Regardless of the size of the gas-phase liquid nitrogen tank, it is equipped with an intelligent control system that can display the internal temperature, perform relevant alarms, and automatically replenish liquid nitrogen.

The liquid nitrogen tank is a low-temperature container that can only store liquid nitrogen and cannot store other liquids. It is mainly composed of inner and outer gallbladders, neck tubes, neck plugs, and buckets. They play different functions in different positions. So what functions does our liquid nitrogen tank neck plug have? What will happen if we accidentally forget to cover the neck plug during operation? You should know that the neck plug is made of polyurethane foam and is used to block the neck of the liquid nitrogen container to reduce the evaporation of liquid nitrogen and play a role in preserving liquid nitrogen. It is light and pressure-free, not easy to break, does not fall off, and can also fix the bucket without adsorbing liquid nitrogen. If you forget to cover the neck plug during use,there will be three situations,the third situation is dangerous. 1. Liquid nitrogen volatilizes in large quantities Liquid nitrogen is a low-temperature liquid that is easily volatile at -196�°C.It will evaporate more quickly when exposed to heat. The liquid nitrogen tank is designed to better isolate heat,so that liquid nitrogen can be stored for a long time.The neck plug is made of polyurethane foam, which is not easy to break and has good thermal insulation properties and can isolate most of the heat. If you forget to cover the neck plug,hot air will enter through the mouth of the tank and accelerate the evaporation of liquid nitrogen.If there are samples stored in the tank,the safety of the samples will be affected and the samples may become inactive. Therefore,when we find that we have forgotten to cover the neck plug,we must first measure the height of the liquid nitrogen in the tank,refill it,and then cover the neck plug. 2. Liquid nitrogen will be contaminated Another function of the neck plug is to prevent impurities or bacteria from falling into the tank. If you forget to cover the neck plug, bacteria and impurities will inevitably fall into the liquid nitrogen tank and contaminate the liquid nitrogen and stored biological samples. 3. Decreased indoor oxygen content Usually, liquid nitrogen tanks are placed indoors. Liquid nitrogen will volatilize more quickly when heated. If the room is not well ventilated, the nitrogen content in the air will increase and the oxygen content will decrease, which may cause the risk of "suffocation". Therefore, Liquid nitrogen tanks stored indoors must be well ventilated. If you have any needs, please contact KGSQ liquid nitrogen tank:

In laboratories and scientific research institutes, experimental samples and cells often need to be stored in a low-temperature environment for a long time. There are many cryogenic containers on the market, and laboratory liquid nitrogen tanks stand out and are entrusted with reuse. This is because the laboratory liquid nitrogen tank has such advantages. It is made of aviation aluminum, which has strong hardness, light weight, long-term durability and no deformation; large-diameter design facilitates sample pickup and placement; large capacity, stable and safe storage of samples; multi-layer insulation Vacuum design effectively reduces the volatilization of liquid nitrogen; it can meet the different needs of different workers. Although, there are ways to use laboratory liquid nitrogen tanks. 1. Before use It should be placed in a well-ventilated room, away from fire sources, electrical equipment, moisture-proof, and shaded; Avoid storing in places where the temperature is too high or too low, and the humidity is too high or too low; Check the sealing of the liquid nitrogen tank to see if there is air leakage. If there is any abnormality, contact after-sales service in time; 2. When using Use caution when adding liquid nitrogen. New jars should be filled slowly and pre-cooled to prevent the liner from cooling too quickly and reducing its service life; When filling liquid nitrogen, do not pour liquid nitrogen on the vacuum exhaust port to avoid a decrease in vacuum degree; The neck plug is made of thermal insulation material, which can not only prevent the evaporation of liquid nitrogen, but also play a role in fixing the lifting cylinder; Check frequently. If frost is found on the surface of the tank, stop using it; 3.After use Liquid nitrogen tanks should be placed on a stable and flat ground, away from direct sunlight and high temperature environments; When not in use for a long time, it should be cleaned and stored to prevent sample residue from corroding the tank wall and affecting the service life; It is recommended to place it in a well-ventilated area and regularly check the insulation performance of the tank; In short, the role of liquid nitrogen tanks in the laboratory is irreplaceable, but their correct use is crucial. During the operation, you must not only pay attention to the storage of the liquid nitrogen tank, but also the addition and correct use of liquid nitrogen to ensure the safety of staff and samples.

Frozen embryos are one of the prerequisites for implementation, the other is the cultivation of embryos. After the eggs and sperm are completely extracted, in order to ensure the viability of the eggs and sperm, they will immediately enter the cryopreservation stage, suspend their life activities, and then complete insemination in an external culture system to complete the embryo development. After the embryo is transplanted into the uterus, it still needs to be stored at low temperature. The method of preservation is to put it into a low-temperature medium - liquid nitrogen. Since the temperature of liquid nitrogen is much lower than normal temperature, organisms in direct contact will be frozen. Otherwise, the plastic will be frozen brittle. In order to ensure the vitality of the sperm and egg "combination" embryo, a special liquid nitrogen container is needed to store it. We can also call it an embryo storage tank, because it is used for embryo storage at this time. The embryo storage tank is composed of two layers: an inner and outer bladder. The inner and outer layers are insulated by a vacuum. It has good thermal insulation and can reduce liquid nitrogen consumption. Even so, the liquid nitrogen balance and temperature in the tank need to be tested every day to ensure that the ambient temperature of the cryopreserved embryos is normal and stable. In order to ensure the safety of the embryos and improve work efficiency, we can install a liquid level alarm, which can detect the liquid level in the tank. When the ambient temperature reaches the upper and lower limits, an alarm will sound to remind the staff to replenish liquid nitrogen in time. It also supports text messages. , telephone, and email alarm functions. The addition of a liquid level alarm can avoid embryo loss caused by insufficient liquid nitrogen. This safety measure ensures the safety of the embryonic baby to a certain extent, preventing the embryonic baby from being harmed by the external environment and receiving the same careful care as in the mother's body. It not only improves the success rate of IVF, but also improves the efficiency of laboratory work management. Moreover, using embryo storage tanks to freeze embryos ensures their vitality during the period between the completion of cultivation and delivery to the mother, reducing damage to the embryos due to temperature changes, etc., and ensuring the success rate of IVF.

As we all know, liquid nitrogen is a low-temperature liquid and is volatile. Laboratories usually use the low temperature of liquid nitrogen to preserve experimental samples. The use of liquid nitrogen tanks can effectively reduce the volatilization of liquid nitrogen, but over time during use, the liquid nitrogen gradually decreases. If cell samples are stored below the level of liquid nitrogen or within the gas phase far away from the tank mouth, it is safe for the samples not to be affected by temperature fluctuations. However, when the liquid nitrogen is less than 1/3 of the total amount, the vaporization time of the liquid nitrogen will increase, and the temperature at the top of the liquid nitrogen tank will rise rapidly as the lid is opened, putting cell activity at risk. During this period, if the liquid nitrogen is not replenished in time, or if the liquid nitrogen is completely evaporated before being replenished, and the temperature of the liquid nitrogen tank rises to normal temperature, the cell samples will not only be damaged, but will most likely die! In order to avoid such problems, laboratory users often use YDD cell bank liquid nitrogen tanks, also known as stem cell liquid nitrogen tanks, to preserve cells and freeze semen samples. The YDD stem cell liquid nitrogen tank itself has an intelligent monitoring and management system. Its functions cover liquid level monitoring. In addition to displaying the liquid level in real time, when the liquid level is lower or higher than the set value, an audible and visual warning will be issued, accompanied by a text message. Send and never miss a message, even remotely. If liquid nitrogen is not added in time, this function is triggered and the manager will receive relevant prompts. Samples will not be affected for a short period of time. In addition, the YDD stem cell liquid nitrogen tank supports two storage modes: gas phase and liquid phase. When using the gas phase mode to store biological samples, the bottom liquid nitrogen volatilizes into nitrogen gas and then supplies the top sample. Liquid nitrogen is not directly related to the sample, and liquid nitrogen cannot soak the sample. , the sample may be damaged. However, in order to ensure the safety of cell samples, it is recommended that you regularly observe the use of liquid nitrogen and refill the jar with liquid nitrogen in time! If you have any needs, please contact KGSQ liquid nitrogen tank:

At present, liquid nitrogen tanks for artificial insemination have been widely used in the reproduction of horses, cattle, pigs, sheep, dogs and other animals. They can effectively preserve and prolong the activity of sperm, ensure the success rate of mating, and are suitable for a variety of animals. The deep low temperature environment (-196â??) of the liquid nitrogen container can keep the activity of sperm stored in it for many years at a high level after thawing, which is particularly important for animal husbandry. The following is a detailed introduction to the advantages of liquid nitrogen tanks for artificial insemination in animal husbandry: 1. The tank body is usually made of high-strength aviation aluminum or aluminum alloy. This material is not only strong and durable, but also makes the tank body have good impact resistance and corrosion resistance, and can adapt to the complex storage environment in animal husbandry. â?? 2. The high vacuum multi-layer insulation design can greatly reduce the evaporation loss of liquid nitrogen, thereby improving the economy. â??Another benefit of the insulation design is that it can maintain a low temperature environment for a long time, ensuring that the stored biological samples or embryos are kept under suitable low temperature conditions for a long time. â?? 3. Equipped with a protective cover and a locking cover, it can prevent bumps and dents during use, protect the safety of stored samples, prevent loss, and increase the safety of the liquid nitrogen tank. â?? 4. The volume of 2-50 liters is small and light, easy to carry, suitable for long-term storage of frozen semen under static conditions, and convenient for users to sample, providing convenience of use. Therefore, liquid nitrogen tanks for artificial insemination play an important role in animal husbandry. It can provide a stable low-temperature environment to ensure the quality and activity of animal semen. It is easy to operate, economical and efficient. It is suitable for a variety of application scenarios and provides an effective solution for the long-term storage and transportation of biological samples.

The use of liquid nitrogen tanks covers scientific research, animal husbandry, gourmet food, beauty, refrigeration accessories and other industries, but it is mainly used for the preservation of biological samples. Preserved biological samples can be subdivided into two major categories, animal semen and experimental cells. The two usages are suitable for different containers and each has its own characteristics. Frozen semen storage should be used with a round bucket; in addition to different accessories, cell storage also has many differences such as capacity and caliber! So what are the characteristics of laboratory liquid nitrogen tanks? First, the capacity must be large; Most of the cells preserved in the laboratory are needed for project research or scientific research, and they are large in number and of various types. They need to be stored stably for a long time, and small-capacity jars have limited storage space, making it difficult to store them in categories and difficult to find. Therefore, laboratories use cell storage tanks of more than 30 liters. Secondly, the diameter must be large and equipped with a freezing rack; As mentioned earlier, there are many types and quantities of experimental cells. In order to facilitate experimenters to find and manage samples, they can be used in conjunction with square buckets to store cells in a regular manner and make them easy to find. Correspondingly, it is also subject to the restrictions of square buckets, and a wide-neck liquid nitrogen tank with a mouth of 125mm or more must be used. Finally, it is equipped with a locking cover to ensure cell safety; Laboratory personnel come and go. For the sake of sample safety, to avoid accidentally taking or losing them, they are usually equipped with locking covers and safety locks to ensure cell safety. Note: The lock cover of the cell storage tank is standard, but the lock needs to be purchased by yourself!

A self-pressurizing liquid nitrogen tank increases the pressure inside the tank through vaporization of liquid nitrogen. When it reaches a certain pressure value, liquid nitrogen can be continuously discharged. Some users of self-pressurizing liquid nitrogen tanks find that the tank cannot discharge liquid nitrogen after using it for a period of time. After self-inspection, they find that the tank is intact. So what is the reason why the tank cannot discharge liquid nitrogen? Reason 1: There is no liquid nitrogen in the tank There is no liquid nitrogen in the tank, so of course it cannot be discharged. The self-pressurizing liquid nitrogen tank itself relies on the vaporization of a small amount of liquid nitrogen to achieve pressurization. Without liquid nitrogen, pressure cannot be generated! Moreover, self-pressurizing liquid nitrogen tanks and liquid nitrogen storage tanks are the same. Even if the tank is filled with liquid nitrogen and left unused, it will naturally evaporate. Therefore, when you find that the self-pressurizing liquid nitrogen tank cannot drain, you must first check whether the liquid nitrogen in the tank has been used up, and replenish it in time. If you only focus on the inability to drain the liquid and do not pay attention to the remaining amount of liquid nitrogen in the tank, this problem will occur a second time. Reason two: ice blockage phenomenon This situation usually occurs at the booster pipe, and sometimes at the drain pipe. This is because the moisture in the air enters the container and is not fully eliminated. When it comes into contact with low temperatures, it will freeze and block the container. Pipes naturally cannot drain fluid.

The stainless steel liquid nitrogen tank is also a self-pressurizing liquid nitrogen tank. The tank itself has a pressurizing system and is adjusted through a pressurizing valve. When the booster valve opens, the pressure increases, and when it closes, the pressure decreases. During use, what is the reason why the booster valve is opened but unable to boost pressure? The reasons why the pressure does not rise after the booster valve of the self-pressurized liquid nitrogen tank is opened are as follows: 1. The booster valve is damaged When the vent valve and the inlet and outlet valve are closed, and the pipeline is unobstructed but does not pressurize, it means that there is a problem with the booster valve itself and the booster valve needs to be replaced. You can contact the manufacturer for replacement. 2. Pipe blockage The main reason for pipeline blockage is that external air and moisture enter and are not discharged in time. Under the influence of low temperature in the tank, they condense into ice and block the pipeline. The correct way to clear the pipe is to use hot nitrogen or hot air to blow away the ice and clear the pipe. After unblocking, it is recommended to transport liquid nitrogen to test whether it is unblocked. Do not use tapping to unblock. 3. Insufficient pressure Check whether the pressure in the liquid nitrogen tank is sufficient. If the pressure in the liquid nitrogen tank is too low, the pressure cannot be increased even if the pressure increase valve is opened. 4. Container leakage The container leaks and the vacuum is destroyed, which will also result in the inability to pressurize. It is easy to judge whether the container is leaking. You can observe the frosting phenomenon on the tank body. The location of a large area of frost is the leakage location. At this time, the jar can no longer be used normally. You can contact the manufacturer for after-sales treatment. In short, there are many reasons why the pressure does not rise after the booster valve of the self-pressurizing liquid nitrogen tank is opened. Check them one by one to find out and solve the problems.