Frozen embryos are one of the prerequisites for implementation, the other is the cultivation of embryos.
After the eggs and sperm are completely extracted, in order to ensure the viability of the eggs and sperm, they will immediately enter the cryopreservation stage, suspend their life activities, and then complete insemination in an external culture system to complete the embryo development. After the embryo is transplanted into the uterus, it still needs to be stored at low temperature. The method of preservation is to put it into a low-temperature medium - liquid nitrogen. Since the temperature of liquid nitrogen is much lower than normal temperature, organisms in direct contact will be frozen. Otherwise, the plastic will be frozen brittle. In order to ensure the vitality of the sperm and egg "combination" embryo, a special liquid nitrogen container is needed to store it. We can also call it an embryo storage tank, because it is used for embryo storage at this time.
The embryo storage tank is composed of two layers: an inner and outer bladder. The inner and outer layers are insulated by a vacuum. It has good thermal insulation and can reduce liquid nitrogen consumption. Even so, the liquid nitrogen balance and temperature in the tank need to be tested every day to ensure that the ambient temperature of the cryopreserved embryos is normal and stable.
In order to ensure the safety of the embryos and improve work efficiency, we can install a liquid level alarm, which can detect the liquid level in the tank. When the ambient temperature reaches the upper and lower limits, an alarm will sound to remind the staff to replenish liquid nitrogen in time. It also supports text messages. , telephone, and email alarm functions. The addition of a liquid level alarm can avoid embryo loss caused by insufficient liquid nitrogen.
This safety measure ensures the safety of the embryonic baby to a certain extent, preventing the embryonic baby from being harmed by the external environment and receiving the same careful care as in the mother's body. It not only improves the success rate of IVF, but also improves the efficiency of laboratory work management.
Moreover, using embryo storage tanks to freeze embryos ensures their vitality during the period between the completion of cultivation and delivery to the mother, reducing damage to the embryos due to temperature changes, etc., and ensuring the success rate of IVF.
In laboratories and scientific research institutes, experimental samples and cells often need to be stored in a low-temperature environment for a long time. There are many cryogenic containers on the market, and laboratory liquid nitrogen tanks stand out and are entrusted with reuse. This is because the laboratory liquid nitrogen tank has such advantages. It is made of aviation aluminum, which has strong hardness, light weight, long-term durability and no deformation; large-diameter design facilitates sample pickup and placement; large capacity, stable and safe storage of samples; multi-layer insulation Vacuum design effectively reduces the volatilization of liquid nitrogen; it can meet the different needs of different workers. Although, there are ways to use laboratory liquid nitrogen tanks. 1. Before use It should be placed in a well-ventilated room, away from fire sources, electrical equipment, moisture-proof, and shaded; Avoid storing in places where the temperature is too high or too low, and the humidity is too high or too low; Check the sealing of the liquid nitrogen tank to see if there is air leakage. If there is any abnormality, contact after-sales service in time; 2. When using Use caution when adding liquid nitrogen. New jars should be filled slowly and pre-cooled to prevent the liner from cooling too quickly and reducing its service life; When filling liquid nitrogen, do not pour liquid nitrogen on the vacuum exhaust port to avoid a decrease in vacuum degree; The neck plug is made of thermal insulation material, which can not only prevent the evaporation of liquid nitrogen, but also play a role in fixing the lifting cylinder; Check frequently. If frost is found on the surface of the tank, stop using it; 3.After use Liquid nitrogen tanks should be placed on a stable and flat ground, away from direct sunlight and high temperature environments; When not in use for a long time, it should be cleaned and stored to prevent sample residue from corroding the tank wall and affecting the service life; It is recommended to place it in a well-ventilated area and regularly check the insulation performance of the tank; In short, the role of liquid nitrogen tanks in the laboratory is irreplaceable, but their correct use is crucial. During the operation, you must not only pay attention to the storage of the liquid nitrogen tank, but also the addition and correct use of liquid nitrogen to ensure the safety of staff and samples.
The liquid nitrogen tank is a low-temperature container that can only store liquid nitrogen and cannot store other liquids. It is mainly composed of inner and outer gallbladders, neck tubes, neck plugs, and buckets. They play different functions in different positions. So what functions does our liquid nitrogen tank neck plug have? What will happen if we accidentally forget to cover the neck plug during operation? You should know that the neck plug is made of polyurethane foam and is used to block the neck of the liquid nitrogen container to reduce the evaporation of liquid nitrogen and play a role in preserving liquid nitrogen. It is light and pressure-free, not easy to break, does not fall off, and can also fix the bucket without adsorbing liquid nitrogen. If you forget to cover the neck plug during use,there will be three situations,the third situation is dangerous. 1. Liquid nitrogen volatilizes in large quantities Liquid nitrogen is a low-temperature liquid that is easily volatile at -196�°C.It will evaporate more quickly when exposed to heat. The liquid nitrogen tank is designed to better isolate heat,so that liquid nitrogen can be stored for a long time.The neck plug is made of polyurethane foam, which is not easy to break and has good thermal insulation properties and can isolate most of the heat. If you forget to cover the neck plug,hot air will enter through the mouth of the tank and accelerate the evaporation of liquid nitrogen.If there are samples stored in the tank,the safety of the samples will be affected and the samples may become inactive. Therefore,when we find that we have forgotten to cover the neck plug,we must first measure the height of the liquid nitrogen in the tank,refill it,and then cover the neck plug. 2. Liquid nitrogen will be contaminated Another function of the neck plug is to prevent impurities or bacteria from falling into the tank. If you forget to cover the neck plug, bacteria and impurities will inevitably fall into the liquid nitrogen tank and contaminate the liquid nitrogen and stored biological samples. 3. Decreased indoor oxygen content Usually, liquid nitrogen tanks are placed indoors. Liquid nitrogen will volatilize more quickly when heated. If the room is not well ventilated, the nitrogen content in the air will increase and the oxygen content will decrease, which may cause the risk of "suffocation". Therefore, Liquid nitrogen tanks stored indoors must be well ventilated. If you have any needs, please contact KGSQ liquid nitrogen tank:
Features: High strength and high corrosionproof, provides long service life and convenient to fill and clean. It provides wide applicability and can be widely applied for packaging in industries such as chemical engineering, petroleum, medicine and food etc. Seal liquid test:Put the sealed barrel with liquid upside down or tap,the seal is at the lowest position,and no leakage within 24 hours. The standard specifies blow-moulding technology requirements of the barrel, test methods and the rules and symbols, packaging, transporting and storage. This standard applies to polyethylene as the main raw material, barrels production through automatic blow molding machine,and this type of blow molding barrel is mainly used for solids or chemical products. We also can help to change any design as your requirements. such as size,weight,color,capacity ect.