The Nucleic Acid Isolation Kit (Spin column method) is designed for purification of RNA and DNA from body fluids (such as swabs, plasma, serum) using nucleic acid extraction instruments.
Mag-COOH and Mag-OH are special ferrimagnetic, silica beads,developed for use in microfluidic and chip-based genomic setups. Intended for nucleic acid isolation from various sources(blood,cells,bacteria etc.) for manual and automated work-flow. Under specific conditions, the carboxylated or hydrolyzed beads surface enables higher yield and purity from samples.
Ferrimagnetic core with silica shell
Small bead diameter, providing a large active silica surface
High yields and good nucleic acids quality
Instant collection of ferrimagnetice beads even in a weak magnetic field
Low magnetic remanance allowing easy resuspension
This product is intended to facilitate release of nucleic acid from cells or other materials containing genetic materials in a form suitable for amplification.
The Kit provides a rapid and easy method for the isolation of viral RNA and DNA from plasma, serum, and cellfree body fluids using silica membrane technology.
Description
The Kit provides a rapid and easy method for the isolation of viral RNA and DNA from plasma, serum, and cellfree body fluids. It is based on silica membrane technology and provides special buffer system. A specially formulated buffer system allows nucleic acid to bind to the membrane. Samples are lysed under denaturing conditions and then transferred to the nucleic acid column where nucleic acid binds and cellular debris, hemoglobin, and other proteins are washed away. High-quality nucleic acid is eluted in nuclease-free water. Phenol extraction and ethanol precipitation are not required. nucleic acid purified can be directly used in downstream molecular biology experiments such as PCR and real time qPCR, and next generation sequencing.
Keys
*Â The kit can extract viral RNA and DNA simply and fast by adopting the silica membrane spin column.
* Safe and non-toxic because there's no need for phenol/chloroform extraction.
* High-quality viral RNA and DNA can be obtained within 45 minutes.
* Complete removal of contaminants and PCR inhibitors for downstream experiments.
Features
Specification
Starting Amount - 200 ul
Starting Material - Plasma, serum, and other acellular body fluids
Elution Volume - 30-150
Technology - Column membrane technology
Processing Mode - Manual
Throughput - 1-24 samples/run
Storage - Room temperature (15-25)
Applications - PCR and real time qPCR, and next generation sequencing.
Especially for Virus infectious diseases analysis.
