The chemical name of the biological buffer HEPES product is: 4-hydroxyethylpiperazine ethanesulfonic acid, a white crystalline powder. It is a weak acid and a zwitterionic buffer in organic chemicals. The decomposition ability of the substance can increase with the increase of temperature, and decrease with the decrease of temperature. However, compared with other buffers, its decomposition constant does not change much with temperature. This makes HEPES buffers more effective at low temperatures. A buffer to keep the structure and function of the enzyme well. It is widely used in many fields such as clinical laboratory diagnostic reagents, life science research, pharmacy and biotechnology.
1) The research of biological buffer HEPES on promoting the stability of foot-and-mouth disease antigen.
Foot-and-mouth disease antigen is easily degraded during storage and vaccine preparation, which affects the vaccine effect. The degradation rate of the foot-and-mouth disease antigen is related to the stability of the pH value of the maintenance solution. The preparation of a maintenance solution with strong pH stability can improve the quality of the foot-and-mouth disease vaccine.
2) Preparation of a skin care product containing a biological buffer, which contains a microbial fermentation product extract, which is characterized in that it also contains a biological buffer with a mass percentage of 0.1%-3.0%. Advantages: The pH value of the product has a small drift range. Within three years after the preparation of the skin care product, the pH value can be stabilized at 6.0-8.0, which has a good protection for the microbial fermentation product extract added therein, and the microbial fermentation product extract is not Easily hydrolyzed, the anti-aging effect of the product lasts for a long time.
3) Prepare a neuron-specific enolase content detection kit, which belongs to the field of human neuron-specific enolase content detection.
This detection kit has high sensitivity and a wide detection linear range, and has the advantages of simple operation, rapidity, strong specificity, good stability and high sensitivity.
In addition to HEPES, the biological buffers produced by Desheng include Tris, Bicine, Caps, Mops, Taps, EPPS, etc.; they are mainly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. Customers in need can provide samples.
Since the outbreak of the epidemic, relevant diagnostic reagent R&D and production companies have introduced RT-PCR nucleic acid detection kits for the first time. By collecting oropharyngeal swab or nasopharyngeal swab samples, the RNA of the virus is extracted and purified for qualitative or quantitative detection. "2019 -New Coronavirus". The sample collection and storage container used in this method is a "disposable virus sampling tube". The new coronavirus nucleic acid detection, disposable virus sampling tube can be said to be indispensable. Next, Desheng intends to introduce the composition and use of the virus sampling tube.
About the composition and manufacturing of the virus sampling tube:
1. Sampling swab: The sampling swab directly contacts the sampling site, and the material of the sampling head is closely related to the subsequent detection.
2. Virus preservation solution: There are two main types of virus preservation solution widely used in the market. One is the improved virus maintenance solution based on the delivery medium, and the other is the improved preservation solution of nucleic acid extraction lysis solution.
3. Preservation tube: The material of the preservation tube should be selected carefully. There are data indicating that Polypropylene is related to the adsorption of nucleic acids, especially when the ion concentration of high tension is high, polyethylene plastic (Polyethylene) is better than polypropylene (Polypropylene). Easy to grasp DNA/RNA.
About the use of virus sampling tube:
The virus sampling tube sampling is mainly divided into oropharyngeal sampling and nasopharyngeal sampling:
1. Oropharyngeal sampling: first press the tongue with a tongue depressor, and then put the sampling swab head into the throat to wipe the bilateral pharyngeal tonsils and back wall of the pharynx, wipe the back wall of the pharynx with light force, avoid touching the tongue unit.
2. Nasopharyngeal sampling: use a swab to measure the distance from the tip of the nose to the earlobe and mark it with your fingers. Insert the sampling swab into the nasal cavity in the direction perpendicular to the nose (face). The distance of the swab should be at least half the length of the earlobe to the tip of the nose. Leave the swab in the nose for 15-30 seconds, gently rotate it 3-5 times, and withdraw the swab.
It is not difficult to see from the method of use that whether it is an oropharyngeal swab or a nasopharyngeal swab, sampling is a technical task, which is difficult and easy to contaminate. The quality of the collected sample is directly related to the subsequent testing. If the viral load of the collected sample is Low, easy to cause false negatives, difficult to diagnose.
please contact me if you need Korean Rapid/RT-PCR detect Kit
(rapid: Genbody, SD biosensor, Wellsbio, Boditech, Bionote, Nanoentek)
(RT-PCR: Wellsbio, GeneMatrix, SD Biosensor)
GeneFinderTM COVID-19 Plus RealAmp Kit is the One- Step Reverse Transcription Real-Time PCR Kit designed to detect Novel Corona virus (COVID-19) qualitatively through Reverse Transcription reaction and Real-Time Polymerase Chain Reaction
1 Kit ( 100 Units )
Main Features
Target Genes : RdRp, N, E
120 minutes detection for COIVD-19
Reverse Transcription reaction and Real-
Time Polymerase Chain Reaction
Easy-to-use(One-Tube) and interpretation
Reliable result by internal/Positive/
Negative Control
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Testing kit for New coronavirus (2019-nCOV) IgG/IgM antibody Rapid Test WB/S/P
Specifications
25 tests
Introduce
This kit uses the colloidal gold immunochromatographic principle to qualitatively detect novel coronavirus igg/igm antibodies in human serum, plasma, or whole blood. the sample to be tested was added to the sample hole of the test paper, and then added 2 drops of sample diluent. the igg/igm antibody in the sample combined with the recombinant new coronavirus antigen labeled with the colloidal gold on the binding pad formed complex. under the action of chromatography, it diffused forward along the nitrocellulose membrane (nc membrane). the nitrocellulose membrane was fixed with two detection lines (g line, m line) and one quality control line (c line). the g line was fixed with anti-human igg antibody for the detection of new coronavirus igg antibody; the m line was fixed with anti-human igm antibody for the detection of new coronavirus igm antibody; and the c line had quality control for the detection of the new coronavirus ig antibody.
If the sample contains a new coronavirus IgG antibody, then the antibody binds to the recombinant new coronavirus antigen labeled with colloidal gold, diffuses forward, and then binds to the anti-human IgG antibody fixed on the NC membrane detection line (G line). if the sample contains a novel coronavirus igm antibody, then the antibody binds to the recombinant new coronavirus antigen labeled with colloidal gold, diffuses forward, and then binds to the anti-human igm antibody fixed on the nc membrane detection line (m line). The darker the color of the colloidal gold accumulated on the detection line, the higher the concentration of the new coronavirus antibody in the sample. To monitor the effectiveness of the test card, set up a quality control line (line C), whether there is a detection line (line T), quality control line (line C) should be color, otherwise the test results are judged invalid.
Testing kit for New coronavirus (2019-nCOV) IgG/IgM antibody Rapid Test WB/S/P
Specifications
25 tests
Introduce
This kit uses the colloidal gold immunochromatographic principle to qualitatively detect novel coronavirus igg/igm antibodies in human serum, plasma, or whole blood. the sample to be tested was added to the sample hole of the test paper, and then added 2 drops of sample diluent. the igg/igm antibody in the sample combined with the recombinant new coronavirus antigen labeled with the colloidal gold on the binding pad formed complex. under the action of chromatography, it diffused forward along the nitrocellulose membrane (nc membrane). the nitrocellulose membrane was fixed with two detection lines (g line, m line) and one quality control line (c line). the g line was fixed with anti-human igg antibody for the detection of new coronavirus igg antibody; the m line was fixed with anti-human igm antibody for the detection of new coronavirus igm antibody; and the c line had quality control for the detection of the new coronavirus ig antibody.
If the sample contains a new coronavirus IgG antibody, then the antibody binds to the recombinant new coronavirus antigen labeled with colloidal gold, diffuses forward, and then binds to the anti-human IgG antibody fixed on the NC membrane detection line (G line). if the sample contains a novel coronavirus igm antibody, then the antibody binds to the recombinant new coronavirus antigen labeled with colloidal gold, diffuses forward, and then binds to the anti-human igm antibody fixed on the nc membrane detection line (m line). The darker the color of the colloidal gold accumulated on the detection line, the higher the concentration of the new coronavirus antibody in the sample. To monitor the effectiveness of the test card, set up a quality control line (line C), whether there is a detection line (line T), quality control line (line C) should be color, otherwise the test results are judged invalid.
Storage
Stored at 4 to 30c and valid for 18 months.
After the aluminum foil bag is unsealed, please use it as soon as possible.
Sample
1. It is suitable for human serum, plasma and whole blood samples.
2. The samples should be used as soon as possible; if they cannot be used immediately, the serum / plasma samples should be kept at 2-8C for 5 days and -20C for long-term preservation; the whole blood samples should be placed at 2-8C to avoid hemolysis;
3.The sample contains a large amount of lipids, hemolysis or turbidity, do not use, so as not to affect the judgment of the results.
Sole Distributor for GP COVID-19 IgM&IgG Antibody Detection Kit. Manufactured by a leading listed company in its field. A fast screening tool without the need of lab environment or equipments to tell if a person has been infected by coronavirus. It has been tested in clinical trial with excellent outcome. The whole test lasts only 10-15 minutes. Anyone can do it and anyone should do!
Test Range of 4 SENSORS Kits
The 4 SENSORS test, an enzyme-linked receptor-binding assay that detects the -lactams group & Tetracyclines group & Streptomycin group & Chloramphenicol group , penicillin G, ampicillin, amoxicillin, oxacillin, cloxacillin, dicloxacillin, nafcillin, cefquinome, cefacetrile, cefalonium, cefazolin, cefoperazone, cephapirin, ceftiofur, Tetracycline, Doxycycline, Oxytetracycline, Chlortetracycline, streptomycin, dihydrostrptomycin and chloramphenicol in raw, commingled cow milk at or below established tolerance and/or safe levels.
1. No extra devices or equipments
2. Only 5 mins to results.
3. No need to pre-heat or refrigerate the milk.
4. Ballya holds GOST,CE,ISO certifications.
5. 50-500ppt LOD.
6. Save milk, save time, save energy.
7. Rapid One Step Assay,Cost-effective.
8. Professionals result from 9 years involvement.
9. Long term storage under 2-8â?? (35�°F-46�°F).
This kit uses immunocolloidal gold chromatography to detect novel coronavirus IgM/IgG antibodies in human serum, plasma or whole blood in vitro.
Materials Provided
1.Detection cassette coated novel coronavirus recombinant antigen colloidal storage in dark and dry
Product characteristic
1. Test sample Serum plasma or whole blood
2. Short detection time Rapid detection of novel coronavirus within 15 minutes SARS-CoV-
3. No instrumentation required No need for instruments and equipment,
suitable for rapid screening
4. Suitable for primary screening Screening for suspected novel coronavirus patients
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