Span Biotech Ltd.

R 10 minutes is considered invalid. Interpretation of results Positive: only clear c band appears. Negative: the presence of both c band and t band. Invalid: no colored band appears in c zone regardless of whether t band appears. Specificity The results are negative when the rapid test is applied to detect 100 ppm of chloramphenicol, aminoglycosides, tetracyclines, macrolides and sulfonamides. Storage All materials must be stored at 2-8¡ãc. The test kit is stable through the expiration date (12 months) marked on the foil pouch. Do not store the test kit in direct sunlight. Precaution 1. For best results, please strictly adhere to these instructions. 2. All reagents must be at room temperature before running the assay. 3. Do not remove rapid test from its pouch until immediately before use. 4. The milk sample should be fresh and homogeneous liquid without caking and precipitation. 5. Do not reuse the test strip. 6. Do not use the test beyond its expiration date marked on the foil pouch. 7. Do not use water, powdered milk as negative control. Use confirmed negative fresh milk as control. 8. The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers. Limitation Beta-lactam rapid test is an useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. If you want a quantitative result, it is suggested to apply other method such as elisa / hplc in practice. In house standard

Beta-lactam rapid test Interpretation of results Positive: only clear c band appears. Negative: the presence of both c band and t band. Invalid: no colored band appears in c zone regardless of whether t band appears. Specificity The results are negative when the rapid test is applied to detect 100 ppm of chloramphenicol, aminoglycosides, tetracyclines, macrolides and sulfonamides. Storage All materials must be stored at 2-8¡ãc. The test kit is stable through the expiration date (12 months) marked on the foil pouch. Do not store the test kit in direct sunlight. Precaution 1.For best results, please strictly adhere to these instructions. 2.All reagents must be at room temperature before running the assay. 3.Do not remove rapid test from its pouch until immediately before use. 4.The milk sample should be fresh and homogeneous liquid without caking and precipitation. 5.Do not reuse the test strip. 6.Do not use the test beyond its expiration date marked on the foil pouch. 7.Do not use water, powdered milk as negative control. Use confirmed negative fresh milk as control. 8.The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers. Limitation Beta-lactam rapid test is an useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. If you want a quantitative result, it is suggested to apply other method such as elisa / hplc in practice. Depend on quantity

Feline leukemia virus ag rapid test Eterinarian. For an accurate result, it is suggested to Apply other method such as elisa for final determination in practice. In house standard

Ed. If only one red line appears, it indicates that the cow is not Pregnant, p4 is in the lowest level at this moment. It is recommended to make the best use of this time to Because the diary cow¡¯s estrous cycle is 18-22 days, it is suggested to perform the test the 18th days after . In order to get reliable result, give up the first three Milking and then collect sample milk around 5-10ml. Mix the sample milk well before testing. Urine and blood Positive: if both t band and c band are visible, the test result is positive, which means the cow is pregnant or In middle period of estrous cycle. Negative: if only c line is visible, the test result is negative, which indicates the cow is not pregnant. Invalid: when control line does not appear on the membrane, the test is invalid due to improper test Procedure or deterioration of reagents. It is recommended that the test be repeated. Precaution (1) test according to the law of the estrous cycle strictly. (2) for single use only; do not reuse the test devices. (3) don¡¯t keep the strip or cassette in the air for a long time after unpacking. (4) while performing the test, make sure that the surface of sample should not exceed the ¡°max¡± line of strip. (5) do not use test kit after expiry date. Validity and storage The period of the validity is 24 months. Store as packaged in the sealed pouch under the room temperature. Avoid light. In house standard

1.Do centrifugation to cattle¡¯s whole blood sample to collect serum or plasma for use. 2.Take out the cassette from the foil pouch and place it horizontally. 3.Drip 1 drop of serum into the sample hole ¡°s¡± and immediately drip 1 drop of assay buffer. 4.Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid. Interpretation of results Positive: the presence of both c band and t band, no matter t band is clear or vague. Negative: only clear c band appears. Invalid: no colored band appears in c zone, no matter whether t band appears. Storage The kit can be stored at room temperature (2-30¡ãc). The test kit is stable through the expiration date (18 months) marked on the foil pouch. Do not freeze. Do not store the test kit in direct sunlight. Precaution 1.For best results, please strictly adhere to these instructions. 2.All reagents must be at room temperature before running the assay. 3.Do not remove test cassette from its pouch until immediately before use. 4.Do not reuse the test kit. 5.Do not use the test beyond its expiration date marked on the foil pouch. 6.The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers. Limitation Bovine brucella ab rapid test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. For an accurate result, it is suggested to apply other method such as elisa for final determination in practice. In house standard

4. Centrifugal tubes (each containing 180 ¦ìl of assay buffer) 5. Product manual Test procedure 1. Do centrifugation to cattle¡¯s whole blood sample to collect serum or plasma for use. 2. Use small pipette with reticle to transfer 20 ¦ìl (upper reticle) of serum or plasma into the centrifugal tube with assay buffer. Mix them well by the pipette attached in the pouch. 3. Take out the cassette from the foil pouch and place it horizontally. 4. Drip 3 drops (about 100 ¦ìl) of sample mixture into the sample hole. 5. Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid. Interpretation of results Positive: the presence of both c band and t band, no matter t band is clear or vague. Negative: only clear c band appears. Invalid: no colored band appears in c zone, no matter whether t band appears. Storage The kit can be stored at room temperature (2-30¡ãc). The test kit is stable through the expiration date (18 months) marked on the foil pouch. Do not freeze. Do not store the test kit in direct sunlight. Precaution 1. For best results, please strictly adhere to these instructions. 2. All reagents must be at room temperature before running the assay. 3. Do not remove test cassette from its pouch until immediately before use. 4. Do not reuse the test kit. 5. Do not use the test beyond its expiration date marked on the foil pouch. 6. The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers. Limitation Bovine tuberculosis ab rapid test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. For an accurate result, it is suggested to apply other method such as elisa for final determination in practice. In house standard

Name Testing kit for New coronavirus (2019-nCOV) IgG/IgM antibody Rapid Test WB/S/P Specifications 25 tests Introduce This kit uses the colloidal gold immunochromatographic principle to qualitatively detect novel coronavirus igg/igm antibodies in human serum, plasma, or whole blood. the sample to be tested was added to the sample hole of the test paper, and then added 2 drops of sample diluent. the igg/igm antibody in the sample combined with the recombinant new coronavirus antigen labeled with the colloidal gold on the binding pad formed complex. under the action of chromatography, it diffused forward along the nitrocellulose membrane (nc membrane). the nitrocellulose membrane was fixed with two detection lines (g line, m line) and one quality control line (c line). the g line was fixed with anti-human igg antibody for the detection of new coronavirus igg antibody; the m line was fixed with anti-human igm antibody for the detection of new coronavirus igm antibody; and the c line had quality control for the detection of the new coronavirus ig antibody. If the sample contains a new coronavirus IgG antibody, then the antibody binds to the recombinant new coronavirus antigen labeled with colloidal gold, diffuses forward, and then binds to the anti-human IgG antibody fixed on the NC membrane detection line (G line). if the sample contains a novel coronavirus igm antibody, then the antibody binds to the recombinant new coronavirus antigen labeled with colloidal gold, diffuses forward, and then binds to the anti-human igm antibody fixed on the nc membrane detection line (m line). The darker the color of the colloidal gold accumulated on the detection line, the higher the concentration of the new coronavirus antibody in the sample. To monitor the effectiveness of the test card, set up a quality control line (line C), whether there is a detection line (line T), quality control line (line C) should be color, otherwise the test results are judged invalid. Storage Stored at 4 to 30c and valid for 18 months. After the aluminum foil bag is unsealed, please use it as soon as possible. Sample 1. It is suitable for human serum, plasma and whole blood samples. 2. The samples should be used as soon as possible; if they cannot be used immediately, the serum / plasma samples should be kept at 2-8C for 5 days and -20C for long-term preservation; the whole blood samples should be placed at 2-8C to avoid hemolysis; 3.The sample contains a large amount of lipids, hemolysis or turbidity, do not use, so as not to affect the judgment of the results.

Name Testing kit for New coronavirus (2019-nCOV) IgG/IgM antibody Rapid Test WB/S/P Specifications 25 tests Introduce This kit uses the colloidal gold immunochromatographic principle to qualitatively detect novel coronavirus igg/igm antibodies in human serum, plasma, or whole blood. the sample to be tested was added to the sample hole of the test paper, and then added 2 drops of sample diluent. the igg/igm antibody in the sample combined with the recombinant new coronavirus antigen labeled with the colloidal gold on the binding pad formed complex. under the action of chromatography, it diffused forward along the nitrocellulose membrane (nc membrane). the nitrocellulose membrane was fixed with two detection lines (g line, m line) and one quality control line (c line). the g line was fixed with anti-human igg antibody for the detection of new coronavirus igg antibody; the m line was fixed with anti-human igm antibody for the detection of new coronavirus igm antibody; and the c line had quality control for the detection of the new coronavirus ig antibody. If the sample contains a new coronavirus IgG antibody, then the antibody binds to the recombinant new coronavirus antigen labeled with colloidal gold, diffuses forward, and then binds to the anti-human IgG antibody fixed on the NC membrane detection line (G line). if the sample contains a novel coronavirus igm antibody, then the antibody binds to the recombinant new coronavirus antigen labeled with colloidal gold, diffuses forward, and then binds to the anti-human igm antibody fixed on the nc membrane detection line (m line). The darker the color of the colloidal gold accumulated on the detection line, the higher the concentration of the new coronavirus antibody in the sample. To monitor the effectiveness of the test card, set up a quality control line (line C), whether there is a detection line (line T), quality control line (line C) should be color, otherwise the test results are judged invalid.