Heat Stable Alpha Amylase

Description
Heat-stable Alpha-Amylase HS-400 is derived from Bacillus licheniformis by submerged fermentation and refining extraction process. HS-400 is an endoamylase which can rapidly decrease the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the |¨¢-D-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

HS-400 is applied to the starch liquefaction in ethanol industry.

Characterisitcs
Declared Enzyme: Alpha-Amylase
Systematic Name: 1,4-|¨¢-D-glucan glucanohydrolase
Activity: 40,000 U/ml (minimum)
Appearance: Light to dark brown liquid
Product pH: 5.8 to 6.8
Specific gravity: 1.15 to 1.25 g/ml

EFFECT OF PH
HS-400 is active over a wide range of pH, depending on the application. For maximum activity, the optimal pH is 6.0 to 6.4. At a temperature of 25¡ãC, the stable pH range is 5.0 to 10.0. The actual pH optimum depends on actual process conditions including temperature, substrate concentration and processing time.

EFFECT OF TEMPERATURE
Optimum temperature of HS-400 for interval liquefaction is above 90¡ãC. HS-400 liquefies starch substrates efficiently at 95 to 97¡ãC and is still highly active at 100¡ãC. This product demonstrates exceptional thermal stability at temperatures from 105 to 110¡ãC, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently. At a temperature above 105¡ãC, the enzyme stability decreases rapidly. The exact temperature optimum depends on actual processing conditions including pH, substrate concentration and processing time.







PACKAGING
This enzyme is often packaged in 25kg or 30kg non-hazardous plastic drums, or 1200 kg IBC totes.
Customized packaging is available upon request.

STORAGE
This enzyme has been formulated for optimal stability. Typical shelf-life is six months provided being stored below 25¡ãC in its original packaging, sealed and unopened, protected from the
sunlight. Its shelf-life will be eighteen months if it is stored properly at 0¡«10¡ãC. Prolonged
storage and/or adverse conditions such as higher temperatures may lead to a higher dosage
requirement.