DESCRIPTION
Glucoamylase PUREMEI PG is made from NON-GMO strains of Aspergillus niger through submerged fermentation, extraction and refining processes. PUREMEI PG has a high conversion rate of starchy substrates into fermentable sugars. It can hydrolyze a-D-1, 4 glucosidic bonds from the non-reducing end of starch one after. It can also hydrolyze the a-D-1, 6 glucoside branch bonds of starch and cleaves the a-1, 3 bonds to release glucose.
PUREMEI PG is designed with a robust glucoamylase activity for saccharification in fuel ethanol production. Use of PUREMEI PG provides higher ethanol yields, faster fermentations, and lower residual starch levels.
PRODUCT CHARACTERISTICS
Declared Enzyme: Glucoamylase
Systematic Name: EC 3.2.1.3, 1,4-alpha-D-glucan glucohydrolase
Appearance: Sepia brown liquid
Activity: 130,000 U/ml (minimum)
Product pH: 3.0 to 5.0
Specific gravity: 1.10 to 1.20 g/ml
EFFECT OF PH
The optimal pH for PUREMEI PG is 4.0 to 4.5.
EFFECT OF TEMPERATURE
The effective temperature range for PUREMEI PG is 30 to 65°C. The optimal temperature range is 55 to 60°C.
PRODUCT APPLICATIONS AND BENEFITS
PUREMEI PG is used to saccharify liquified mash from various sources including corn, milo, barley, wheat, rice, potatoes and tapioca. The resultant glucose product is fermented by yeast or another appropriate microorganism to yield ethanol.
For ground-grain processing plants using simultaneous saccharification fermentation (SSF), PUREMEI PG may be added directly to the fermenter. For ground-grain dry mill plants and for wet mill plants using a separate pre-saccharification step prior to fermentation, PUREMEI PG may be added to the cooling mash or liquefied starch when the temperature reaches 55 to 60°C. Temperatures above 65°C can result in loss of activity rapidly.
DOSAGE GUIDELINES
PUREMEI PG is generally added at a level of 0.065 to 0.085% w/w (dry solids basis). The actual dosage depends on the actual processing conditions including raw materials, temperature, reaction time, pH and the desired results in individual ethanol plants.