Cell Culture Media

INCI Name: FIBRONECTIN Appearance: Colorless aqueous solution Purity: 98% CAS NO.: 86088-83-7 Application: cosmetics and skin care products Supplier: ZHENYIBIO Fibronectin,abbreviated as FN; it is a high molecular weight non-collagen glycoprotein with a molecular sugar content of 4.5-9.5% ; Mainly produced by liver and vascular endothelial cells, widely present in plasma, a variety of cell surfaces and cell matrix, the content of FN in plasma is about 0.3mg/ml; it is one of the main components of extracellular matrix. Application of Fibronectin: (1) Fibronectin and cell culture: Fibronectin (FN), as a substrate for cell culture, can increase the attachment rate and confluence rate of a variety of cells, shorten the cell confluence time, make the cell morphology and structure good, increase the metabolic rate, and significantly increase the speed of DNA, RNA and protein synthesis; The colony rate of cells increased, and the survival rate of primary culture increased. (2) Fibronectin and wound repair Fibronectin participates in the whole process of wound repair, especially in the early stage of wound healing. (3) Fibronectin and beauty Thanks to the powerful regeneration and repair function of FN, its application and role in beauty and skin care are also very powerful. It can be used for daily skin care as well as medical beauty, light medical beauty, etc.; it can effectively solve a variety of skin problems such as: Repair of acne, acne, hormonal face, sensitive and weak skin, dry peeling, post-sun damage, post-medical damage and other problematic skin, repair of rough, dull, fine lines, slack and fatigued skin.

Product Name: shikonin Other Name: Alkannin;5,8-Dihydroxy-2-[(1R)-1-hydroxy-4-methyl-pent-3-enyl]naphthalene-1,4-dione Molecular Formula: C16H16O5 Molecular Weight: 288.3 Appearance: Red crystalline solid powder Purity: 30%-99% CAS Number: 517-89-5 Supplier: ZHENYIBIO Shikonin could be found in the plant of Lithospermum erythrorhizon Sieb. et. Zucc and Arnebia euchroma (Royle) Johnst. It could also be produced using plant cell culture techniques. Shikonin is recorded in British Pharmacopoeia as a reference compound. Zicao, a traditional Chinese medicine firstly recorded in Shen Nongâ??s Herbal Classic, has long been used medically in history . Chinese Pharmacopoeia (Edition 2015) recorded the root of Arnebia euchroma (Royle) Johnst and Arnebia guttata Bge as Zicao, which is mainly used for inflammatory diseases such as macular eruptions, measles, sore throat, carbuncles, and burns.

Introduction: 4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells. Application: HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile. Buffer solution preparation: (1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L. (2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4�ºC or -20�ºC. Purpose: 1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA 2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase 3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer

Ferulic acid has a high content in traditional Chinese medicines such as Ferulic acid Angelica sinensis Chuanxiong Cimicifuga and Suanzaoren One is obtained from the combination of ferulic acid and some small molecules the second is obtained from the plant cell wall and the third is obtained through tissue culture Ferulic acid in plants is often crosslinked with polysaccharides and lignin through ester bonds or selfesterified or etherified to form di ferulic acid Generally alkaline and enzymatic methods are used to break the ester bonds to release ferulic acid and then suitable solvents are used for extraction Item Specifications Results Appearance White or lightyellow acicular crystal Passed Melting point 172 01760C 17261748C Loss on drying 050 016 Residue on ignition 010 004 Heavy metals 10PPM Passed Unspecified impurities 010 Not detected Total 050 Not detected Contents 990 1000 Conclusion Conforms