In enzymology, a dtmp kinase (ec 2.7.4.9) is an enzyme that catalyzes the chemical reaction: atp + dtmp rightleftharpoons adp + dtdp. Thus, the two substrates of this enzyme are atp and dtmp, whereas its two products are adp and dtdp. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with a phosphate group as acceptor. This enzyme participates in pyrimidine metabolism.