The liquid nitrogen tank for cell storage is designed for laboratory users. It is a large-capacity low-temperature liquid nitrogen container that can efficiently store biological samples. Made of aviation aluminum, the liquid nitrogen has a low volatility, good thermal insulation performance, and takes up less space. It is an ideal choice for laboratory professionals and can meet different needs. Liquid nitrogen tanks for cell storage have many advantages. Here is a detailed introduction for you: 1. Durability: Made of corrosion-resistant and thermally-insulating aviation aluminum materials, it can resist the influence and damage of the external environment. At the same time, it undergoes strict quality inspection and vacuum testing before leaving the factory to ensure that it meets the standards in various performance indicators. 2. Large-capacity storage space: With a large capacity of 30-175 liters, it is suitable for long-term stable storage of a large number of samples to meet the needs of scientific research experiments. 3. Large-caliber design: The large-caliber design of 125mm, 127mm and 216mm is convenient for sample storage, while maintaining low liquid nitrogen consumption and providing more stable storage conditions. 4. Wide range of applications: Suitable for the preservation of a variety of biological samples, such as cell and tissue samples, microorganisms, seeds, embryos and sperm, etc., as well as for deep freezing treatment of parts and components, etc., with wide application value. 5. Safety: It can be configured with an intelligent monitoring system to achieve remote monitoring, and is equipped with a safety lock cover to prevent others from opening it without authorization, ensuring the safety of cell samples in the container. 6. It can accommodate a large number of cryopreserved samples: It is equipped with a cryopreservation rack and a cryopreservation box, which can accommodate more than 750 2ml cryopreservation tubes, and can reach up to about 6,000. Therefore, due to its unique advantages, the liquid nitrogen tank for cell storage has a wide range of application value in scientific research experiments and industrial production.
At present, liquid nitrogen tanks for artificial insemination have been widely used in the reproduction of horses, cattle, pigs, sheep, dogs and other animals. They can effectively preserve and prolong the activity of sperm, ensure the success rate of mating, and are suitable for a variety of animals. The deep low temperature environment (-196â??) of the liquid nitrogen container can keep the activity of sperm stored in it for many years at a high level after thawing, which is particularly important for animal husbandry. The following is a detailed introduction to the advantages of liquid nitrogen tanks for artificial insemination in animal husbandry: 1. The tank body is usually made of high-strength aviation aluminum or aluminum alloy. This material is not only strong and durable, but also makes the tank body have good impact resistance and corrosion resistance, and can adapt to the complex storage environment in animal husbandry. â?? 2. The high vacuum multi-layer insulation design can greatly reduce the evaporation loss of liquid nitrogen, thereby improving the economy. â??Another benefit of the insulation design is that it can maintain a low temperature environment for a long time, ensuring that the stored biological samples or embryos are kept under suitable low temperature conditions for a long time. â?? 3. Equipped with a protective cover and a locking cover, it can prevent bumps and dents during use, protect the safety of stored samples, prevent loss, and increase the safety of the liquid nitrogen tank. â?? 4. The volume of 2-50 liters is small and light, easy to carry, suitable for long-term storage of frozen semen under static conditions, and convenient for users to sample, providing convenience of use. Therefore, liquid nitrogen tanks for artificial insemination play an important role in animal husbandry. It can provide a stable low-temperature environment to ensure the quality and activity of animal semen. It is easy to operate, economical and efficient. It is suitable for a variety of application scenarios and provides an effective solution for the long-term storage and transportation of biological samples.
The use of liquid nitrogen tanks covers scientific research, animal husbandry, gourmet food, beauty, refrigeration accessories and other industries, but it is mainly used for the preservation of biological samples. Preserved biological samples can be subdivided into two major categories, animal semen and experimental cells. The two usages are suitable for different containers and each has its own characteristics. Frozen semen storage should be used with a round bucket; in addition to different accessories, cell storage also has many differences such as capacity and caliber! So what are the characteristics of laboratory liquid nitrogen tanks? First, the capacity must be large; Most of the cells preserved in the laboratory are needed for project research or scientific research, and they are large in number and of various types. They need to be stored stably for a long time, and small-capacity jars have limited storage space, making it difficult to store them in categories and difficult to find. Therefore, laboratories use cell storage tanks of more than 30 liters. Secondly, the diameter must be large and equipped with a freezing rack; As mentioned earlier, there are many types and quantities of experimental cells. In order to facilitate experimenters to find and manage samples, they can be used in conjunction with square buckets to store cells in a regular manner and make them easy to find. Correspondingly, it is also subject to the restrictions of square buckets, and a wide-neck liquid nitrogen tank with a mouth of 125mm or more must be used. Finally, it is equipped with a locking cover to ensure cell safety; Laboratory personnel come and go. For the sake of sample safety, to avoid accidentally taking or losing them, they are usually equipped with locking covers and safety locks to ensure cell safety. Note: The lock cover of the cell storage tank is standard, but the lock needs to be purchased by yourself!
At present: liquid nitrogen tanks are common low-temperature containers in laboratories and are often used to preserve specimens, samples, cells, etc., which require the constant temperature characteristics of liquid nitrogen tanks. Speaking of which, here comes the question. Many people will ask what size of liquid nitrogen tank should be used in scientific research laboratories? Laboratory liquid nitrogen tanks are generally larger than 30 liters. There are two reasons: 1. Consider capacity The capacity of liquid nitrogen tanks ranges from 2 to 175 liters. Different capacities can store different numbers of samples. The larger the capacity, the more samples can be stored. For laboratories, there are many people who freeze samples, with a wide variety and large quantities. Therefore, it is recommended to choose a jar of more than 30 liters, which can store a variety of samples without the need for frequent refills. 2. Consider caliber There are 6 options for liquid nitrogen tank diameter,50mm,80mm, 125mm, 127mm,210mm,216mm; There are many types and large quantities of samples stored in the laboratory. Freezing racks are often used for matching. If the diameter is too small, it will not fit in. Therefore, it is recommended to use a diameter of 125mm or above. A larger diameter makes it easier to take samples and is used with a freezing box. More samples can be saved. To sum up, liquid nitrogen tanks used in laboratories generally have a capacity of more than 30 liters and a diameter of more than 125mm. But the important thing is to choose based on your actual needs. If you have any needs, please contact KGSQ liquid nitrogen tank:
Currently, the methods of cell preservation are refrigerator and liquid nitrogen. When it comes to long-term storage, liquid nitrogen wins. However, conventional liquid nitrogen tanks need to constantly detect the liquid level, and the cells cannot be stored higher than the liquid nitrogen level, which is not conducive to long-term storage of cells. However, the "stem cell liquid nitrogen tank" that breaks the rules can do it! For long-term storage of large quantities of dry cell samples, it is ideal to use stem cell liquid nitrogen tanks by default. Why? 1. Storage temperature is -150~-196â?? For cells, the more comfortable storage temperature is -150â??~-196â??. Conventional liquid nitrogen tanks only have a temperature option of -196â??. Samples at this temperature are not in danger. The storage temperature of stem cell liquid nitrogen tanks changes with the sample area. The temperature difference in each storage area is 10�°C, which provides more options for cells. You can choose a more suitable temperature for cells to store. 2. Prevent pollution Another important point in cell cryopreservation, stable storage time and how to prevent contamination during storage. To stabilize the storage time, the stem cell liquid nitrogen tank adopts the characteristics of vacuum multi-layer insulation, electronic temperature control and liquid nitrogen dispersion, making the storage tank intelligent cooling and constant temperature process uniform and stable. To prevent storage contamination, you must know that cells become contaminated because they are in direct contact with liquid nitrogen. First, because the cryopreservation tube is not well sealed, liquid nitrogen enters through the gap. After taking out the test tube, there is a temperature difference and pressure difference between the inside and outside. Under the combined action of temperature and pressure, the tube explodes. Second, bacteria will enter the liquid nitrogen tank after it has been used for a period of time. These bacteria are likely to flow into the freezing tube with the liquid nitrogen and contaminate the sample. Stem cell liquid nitrogen tanks can prevent contamination because they can store liquid nitrogen and cells separately, avoiding direct contact between the two. At the same time, they can also use the low-temperature environment formed by the evaporation of liquid nitrogen for storage to avoid pipe explosions and cross-contamination. risk.
The liquid nitrogen tank is a low-temperature container that can only store liquid nitrogen and cannot store other liquids. It is mainly composed of inner and outer gallbladders, neck tubes, neck plugs, and buckets. They play different functions in different positions. So what functions does our liquid nitrogen tank neck plug have? What will happen if we accidentally forget to cover the neck plug during operation? You should know that the neck plug is made of polyurethane foam and is used to block the neck of the liquid nitrogen container to reduce the evaporation of liquid nitrogen and play a role in preserving liquid nitrogen. It is light and pressure-free, not easy to break, does not fall off, and can also fix the bucket without adsorbing liquid nitrogen. If you forget to cover the neck plug during use,there will be three situations,the third situation is dangerous. 1. Liquid nitrogen volatilizes in large quantities Liquid nitrogen is a low-temperature liquid that is easily volatile at -196�°C.It will evaporate more quickly when exposed to heat. The liquid nitrogen tank is designed to better isolate heat,so that liquid nitrogen can be stored for a long time.The neck plug is made of polyurethane foam, which is not easy to break and has good thermal insulation properties and can isolate most of the heat. If you forget to cover the neck plug,hot air will enter through the mouth of the tank and accelerate the evaporation of liquid nitrogen.If there are samples stored in the tank,the safety of the samples will be affected and the samples may become inactive. Therefore,when we find that we have forgotten to cover the neck plug,we must first measure the height of the liquid nitrogen in the tank,refill it,and then cover the neck plug. 2. Liquid nitrogen will be contaminated Another function of the neck plug is to prevent impurities or bacteria from falling into the tank. If you forget to cover the neck plug, bacteria and impurities will inevitably fall into the liquid nitrogen tank and contaminate the liquid nitrogen and stored biological samples. 3. Decreased indoor oxygen content Usually, liquid nitrogen tanks are placed indoors. Liquid nitrogen will volatilize more quickly when heated. If the room is not well ventilated, the nitrogen content in the air will increase and the oxygen content will decrease, which may cause the risk of "suffocation". Therefore, Liquid nitrogen tanks stored indoors must be well ventilated. If you have any needs, please contact KGSQ liquid nitrogen tank:
In laboratories and scientific research institutes, experimental samples and cells often need to be stored in a low-temperature environment for a long time. There are many cryogenic containers on the market, and laboratory liquid nitrogen tanks stand out and are entrusted with reuse. This is because the laboratory liquid nitrogen tank has such advantages. It is made of aviation aluminum, which has strong hardness, light weight, long-term durability and no deformation; large-diameter design facilitates sample pickup and placement; large capacity, stable and safe storage of samples; multi-layer insulation Vacuum design effectively reduces the volatilization of liquid nitrogen; it can meet the different needs of different workers. Although, there are ways to use laboratory liquid nitrogen tanks. 1. Before use It should be placed in a well-ventilated room, away from fire sources, electrical equipment, moisture-proof, and shaded; Avoid storing in places where the temperature is too high or too low, and the humidity is too high or too low; Check the sealing of the liquid nitrogen tank to see if there is air leakage. If there is any abnormality, contact after-sales service in time; 2. When using Use caution when adding liquid nitrogen. New jars should be filled slowly and pre-cooled to prevent the liner from cooling too quickly and reducing its service life; When filling liquid nitrogen, do not pour liquid nitrogen on the vacuum exhaust port to avoid a decrease in vacuum degree; The neck plug is made of thermal insulation material, which can not only prevent the evaporation of liquid nitrogen, but also play a role in fixing the lifting cylinder; Check frequently. If frost is found on the surface of the tank, stop using it; 3.After use Liquid nitrogen tanks should be placed on a stable and flat ground, away from direct sunlight and high temperature environments; When not in use for a long time, it should be cleaned and stored to prevent sample residue from corroding the tank wall and affecting the service life; It is recommended to place it in a well-ventilated area and regularly check the insulation performance of the tank; In short, the role of liquid nitrogen tanks in the laboratory is irreplaceable, but their correct use is crucial. During the operation, you must not only pay attention to the storage of the liquid nitrogen tank, but also the addition and correct use of liquid nitrogen to ensure the safety of staff and samples.
Stem cells are cells with the ability to self-replicate and differentiate into multiple directions. Under certain conditions, they can differentiate into cells with multiple functions and have the ability to repair various tissue functions and regenerate organs. Therefore, they are also called "universal cells." Such important stem cells can only play a greater role if they are preserved intact. Stem cell storage tanks are a common storage method for preserving stem cells. Using liquid nitrogen to place stem cells in a low-temperature environment can effectively extend the storage time and maintain their vitality and function. Since different users have different amounts of stored stem cells, there are three storage tanks to choose from: 1. For liquid phase storage of hundreds or thousands of stem cells, YDS series aviation aluminum liquid nitrogen tanks can be selected; 2. For gas phase storage of thousands of stem cells, small gas-liquid phase stem cell storage tanks can be used, which are also made of aviation aluminum; 3. If the number of stem cells exceeds 10,000, it is more appropriate to use a gas phase stem cell storage tank. Each of these stem cell storage tanks has its own advantages. Liquid phase storage can maintain a constant temperature of -196�°C, but they lack intelligent management. The small gas-liquid phase storage tank is a mini version of the YDD storage tank. It has the same gas-liquid phase dual mode, intelligent management of samples, automatic replenishment and alarm. In addition, the use of aviation aluminum materials with stronger thermal insulation and lighter weight also combines the advantages of YDS. Therefore, when storing stem cells, you must choose an appropriate storage tank based on the number of cells and storage method.
Simmental cattle are an excellent breed of cattle. Livestock farmers will buy Simmental cattle frozen semen and cross-breed them with local cattle to improve the quality of cattle and gain greater breeding benefits! The purchased frozen beef semen cannot be stored in a normal temperature tank. What kind of tank should be used to store it? â??â??Semen storage tank! After Simmental cattle semen is extracted, it will quickly become inactive at room temperature and cannot be used. However, through the semen storage tank, the semen is specially treated and soaked in liquid nitrogen. The deep low temperature of liquid nitrogen -196�° can be used to preserve sperm for a long time. Activity, wait until use before taking out and thawing. Specific steps for thawing frozen cattle semen for breeding: 1. Lift the bucket to the mouth of the jar and use long tweezers to quickly pinch out the frozen wheat tube; 2. Dissolve the frozen wheat tube in warm water at 30-40�°C, and take it out when it is half melted; 3. Check the sperm activity. If the activity is above 0.3, the breeding success rate is high and it can be used for insemination. 4. Load the semen into the gun and output it as soon as possible. Do not store it for a long time. Notice: Insemination time: The appropriate insemination time is after the cowâ??s estrus period, when the breeding success rate is higher. Storage time of frozen semen at room temperature: After thawing, the longer the frozen semen is stored at room temperature, the lower the activity. Therefore, it should be thawed and infused immediately to avoid long-term storage or repeated freezing.
Frozen embryos are one of the prerequisites for implementation, the other is the cultivation of embryos. After the eggs and sperm are completely extracted, in order to ensure the viability of the eggs and sperm, they will immediately enter the cryopreservation stage, suspend their life activities, and then complete insemination in an external culture system to complete the embryo development. After the embryo is transplanted into the uterus, it still needs to be stored at low temperature. The method of preservation is to put it into a low-temperature medium - liquid nitrogen. Since the temperature of liquid nitrogen is much lower than normal temperature, organisms in direct contact will be frozen. Otherwise, the plastic will be frozen brittle. In order to ensure the vitality of the sperm and egg "combination" embryo, a special liquid nitrogen container is needed to store it. We can also call it an embryo storage tank, because it is used for embryo storage at this time. The embryo storage tank is composed of two layers: an inner and outer bladder. The inner and outer layers are insulated by a vacuum. It has good thermal insulation and can reduce liquid nitrogen consumption. Even so, the liquid nitrogen balance and temperature in the tank need to be tested every day to ensure that the ambient temperature of the cryopreserved embryos is normal and stable. In order to ensure the safety of the embryos and improve work efficiency, we can install a liquid level alarm, which can detect the liquid level in the tank. When the ambient temperature reaches the upper and lower limits, an alarm will sound to remind the staff to replenish liquid nitrogen in time. It also supports text messages. , telephone, and email alarm functions. The addition of a liquid level alarm can avoid embryo loss caused by insufficient liquid nitrogen. This safety measure ensures the safety of the embryonic baby to a certain extent, preventing the embryonic baby from being harmed by the external environment and receiving the same careful care as in the mother's body. It not only improves the success rate of IVF, but also improves the efficiency of laboratory work management. Moreover, using embryo storage tanks to freeze embryos ensures their vitality during the period between the completion of cultivation and delivery to the mother, reducing damage to the embryos due to temperature changes, etc., and ensuring the success rate of IVF.
What is a storage tank The design is based on the given working conditions and process size, considering the requirements of manufacturing and installation and maintenance, the correct selection of materials for each component load analysis, stress analysis. Material:carbon steel stainless steel 304, 316L,duplex stainless steel 2205,Titanium etc. Wall thickness: 3mm-20mm Pressure range:0.1-10MPa Layer: single layer,double layer (customizable) Volume:50L-30000L Installation :vertical /horizontal foods Application filed :Food, pharmaceutical industry, chemical industry, dairy products, fruit juice beverages and biological engineering etc. Advantages 1.Easy cleaning :Internal and external mirror polishing,fillet welding of tank no dead angle 2.Easy operation :Humanized structure design 3.Discharge outlet at bottom:It suits the purification area of pharmaceutical industry and the liquid compounding of food or fermentation and the treatment of water . 4.Flexible customization :The sampling place, inlet and outlet of liquid, hole for reservation and inlet and outlet of head/cold medium can all be customized to meet your unique processing and storage needs. � Shandong Qinglei Environmental Technology Co.,Ltd is an equipment manufacturer integrating design, production and assembly. Our products include heat exchangers, heat exchange units, pressure vessels, storage tanks, TCU temperature control systems, reactors, distillation towers, VOCS purification and recovery systems, vacuum� frying machine. If you need any of them,please donot hesitate to let me know,I will reply you ASAP.
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YDS series of liquid nitrogen tanks, in addition to animal husbandry cryopreservation of frozen semen, also has a place in the laboratory, usually used for batch preservation of cell samples, and with the use of freezing racks, so the capacity of liquid nitrogen tanks for laboratory use are more than 30 liters, the caliber â?¥ 125mm, do not need to replenish the liquid nitrogen frequently, and it is convenient to take the cells. There are several kinds of liquid nitrogen tanks commonly used in the laboratory: 1.YDS-30-125, can put 6 freezing racks and about 600 2ml freezing tubes; 1.YDS-47-127, can put 6 square lifting buckets (freezing storage racks) and store about 750 cells; 2.YDS-65-216, 4~6 freezing racks, put about 2000~2400 2ml freezing tubes; 3.YDS-95-216, 6 freezing racks, store about 3000 cells; 4.YDS-115-216, with 6 freezing racks, put 2ml freezing tube about 4000; 5.YDS-145-216, with 6 freezing racks, the number of 2ml freezing tubes to be stored is about 4800; 6.YDS-175-216, 6 freezing shelves, about 2000 2ml frozen tubes. The above models of liquid nitrogen tanks are more often used by laboratories, so they are also called "laboratory liquid nitrogen tanks". This series of liquid nitrogen tanks for laboratory use are made of the same aviation aluminum material, vacuum structure of inner and outer gallbladder, nine temperature-locking process to ensure the heat preservation effect and prolong the liquid nitrogen evaporation time; double-handle design, convenient for indoor transfer, optional fifth wheel trolley and intelligent monitoring system, i.e., liquid nitrogen level monitor/alarm, to realize the remote alarm and temperature monitoring to liberate the laboratory personnel from some of their time. For more information, please contact KGSQ Liquid Nitrogen Tank:
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