The liquid nitrogen tank is a low-temperature container that can only store liquid nitrogen and cannot store other liquids. It is mainly composed of inner and outer gallbladders, neck tubes, neck plugs, and buckets. They play different functions in different positions. So what functions does our liquid nitrogen tank neck plug have? What will happen if we accidentally forget to cover the neck plug during operation? You should know that the neck plug is made of polyurethane foam and is used to block the neck of the liquid nitrogen container to reduce the evaporation of liquid nitrogen and play a role in preserving liquid nitrogen. It is light and pressure-free, not easy to break, does not fall off, and can also fix the bucket without adsorbing liquid nitrogen. If you forget to cover the neck plug during use,there will be three situations,the third situation is dangerous. 1. Liquid nitrogen volatilizes in large quantities Liquid nitrogen is a low-temperature liquid that is easily volatile at -196�°C.It will evaporate more quickly when exposed to heat. The liquid nitrogen tank is designed to better isolate heat,so that liquid nitrogen can be stored for a long time.The neck plug is made of polyurethane foam, which is not easy to break and has good thermal insulation properties and can isolate most of the heat. If you forget to cover the neck plug,hot air will enter through the mouth of the tank and accelerate the evaporation of liquid nitrogen.If there are samples stored in the tank,the safety of the samples will be affected and the samples may become inactive. Therefore,when we find that we have forgotten to cover the neck plug,we must first measure the height of the liquid nitrogen in the tank,refill it,and then cover the neck plug. 2. Liquid nitrogen will be contaminated Another function of the neck plug is to prevent impurities or bacteria from falling into the tank. If you forget to cover the neck plug, bacteria and impurities will inevitably fall into the liquid nitrogen tank and contaminate the liquid nitrogen and stored biological samples. 3. Decreased indoor oxygen content Usually, liquid nitrogen tanks are placed indoors. Liquid nitrogen will volatilize more quickly when heated. If the room is not well ventilated, the nitrogen content in the air will increase and the oxygen content will decrease, which may cause the risk of "suffocation". Therefore, Liquid nitrogen tanks stored indoors must be well ventilated. If you have any needs, please contact KGSQ liquid nitrogen tank:
In biological laboratories,the commonly used low-temperature storage equipment is liquid nitrogen tank,which can be divided into two categories according to the storage mode,one is liquid-phase liquid nitrogen tank, and the other is gas-phase liquid nitrogen tank;however, with the development of science and technology With continuous development,a small gas-liquid phase tank is now designed.To put it simply,it is a combination that draws on the advantages of both. This description is not empty talk,letâ??s take a look below: First of all,it uses the same aviation aluminum as the liquid phase tank as the material,which is high in strength,corrosion-resistant,and relatively lightweight,making it easy to transfer samples. Secondly,judging from its appearance,it not only has a similar appearance to the liquid tank,but also has the same smart display as the gas tank. Its internal structure is similar to that of a gas phase tank and is divided into a liquid nitrogen storage area and a sample storage area. Similarly,there are two storage modes for samples.You can choose the more commonly used liquid phase storage mode,or you can choose the gas phase storage mode like the gas phase tank,which uses volatile nitrogen to preserve the sample.The dual-mode storage mode is also its name.The origin of. The important thing is that small gas-liquid phase tanks have commonly used capacities, such as YDS-65-216,YDS-95-216.This capacity is suitable for users who do not have a particularly large number of cell samples but want to store them in gas phase.Particularly friendly and makes up for the need for small cell storage.Choosing it not only requires less investment than a gas phase tank,but also saves space and can better meet your needs. If you have any needs, please contact KGSQ liquid nitrogen tank:
Liquid nitrogen, as a low-temperature liquid of -196 , cannot be stored in any container. It requires a special container, a liquid nitrogen tank, for storage. Containers for storing liquid nitrogen can be divided into two types: one is an open liquid nitrogen tank, which can store liquid nitrogen and samples; the other is a self-pressurizing liquid nitrogen tank, which is specially used for rehydration of liquid nitrogen containers. Both have the same characteristics, including double-layer structure of inner and outer bladders; vacuum insulation; long-term storage of liquid nitrogen, etc. But apart from that, the difference between the two is still very big, letâ??s look down. 1. Different materials The self-pressurizing tank is made of 304 stainless steel, which has good load-bearing and high strength, and has a smooth metal appearance; The open-top liquid nitrogen tank is made of aluminum alloy, is light in weight and easy to carry. The appearance is spray-painted and is white. 2. Different functions The self-pressurizing tank is used for the storage and transportation of liquid nitrogen. It can increase the pressure through internal liquid nitrogen vaporization and promote liquid drainage and replenishment. It cannot store biological samples; Open liquid nitrogen tank is also called liquid nitrogen biological container. That is: a container for storing liquid nitrogen and biological samples. Samples such as frozen cell semen can be stored in it, and the deep low temperature of liquid nitrogen can be used for long-term preservation. 3. Different structures The self-pressurizing tank as a whole consists of the tank body, valves, pipes, liquid level gauge and internal boosting plate; The open liquid nitrogen tank is composed of inner and outer tanks, vacuum interlayer, neck tube, neck plug and bucket. 4. Different capacities The volume range of self-pressurizing tanks is large, ranging from 15-500L; The volume of open liquid nitrogen tanks is small, ranging from 2-175L. In short, if you need to store biological samples, you can choose a conventional tank; if you refill other liquid nitrogen containers, you need to use a self-pressurizing tank.
The use of liquid nitrogen has become increasingly common in scientific experiments and medical fields. As an ultra-low temperature liquid, it requires special containers, namely liquid nitrogen tanks, for storage and transportation. Liquid nitrogen tanks are a series of low-temperature containers that are made of aviation aluminum and have double-vacuum structures. They can only be used for liquid nitrogen storage or transportation. The advantage is that they are well insulated and can store liquid nitrogen for a long time, so that liquid nitrogen can be used to freeze biological samples. The disadvantage of using it is that it is difficult to move after loading liquid nitrogen and samples, and it is afraid of bumps. The connection between the neck tube and the inner tank cannot withstand shaking. However, in the storage of experimental samples, it is difficult not to involve the issue of indoor transportation. After all, it must serve the experimental subject. then what should we do? Under this demand, the liquid nitrogen tank trolley came into being! It is a tool specially designed for transporting liquid nitrogen containers. It has become an indispensable helper in the laboratory with its advantages of durability, wear resistance, collision resistance and ease of movement. The liquid nitrogen tank trolley is made of 304 stainless steel. The mechanical properties of the steel enable the trolley to maintain core stability when transporting liquid nitrogen containers and not deform due to constant pressure. For some emergencies, such as collision and friction, the wear resistance and collision resistance of stainless steel can effectively reduce the impact on the container and ensure safe transportation. In addition, the trolley is equipped with universal wheels. This user-friendly design makes movement more convenient and allows users to easily transport liquid nitrogen containers from one place to another. This is undoubtedly a great boon for laboratories and medical institutions that need to frequently move liquid nitrogen containers. The emergence of liquid nitrogen tank trolleys has effectively improved transportation efficiency and reduced labor costs. In short, liquid nitrogen tank trolleys have become an essential tool for transporting liquid nitrogen containers due to their durability, wear resistance, collision resistance, and convenience and ease of movement.
The self-pressurizing liquid nitrogen tank is an efficient and safe liquid nitrogen storage equipment, which is widely used in life sciences, medical research and other fields. So, what exactly is it? First, we need to understand how a self-pressurizing liquid nitrogen tank works. It vaporizes a small amount of liquid nitrogen in the tank, causing the internal pressure of the entire container to increase. When the pressure inside the container reaches the critical pressure, the drain valve needs to be opened to realize the continuous drain function. It is specially used to replenish liquid nitrogen in laboratory cryogenic containers to ensure the smooth progress of experiments. Secondly, the safety of self-pressurizing liquid nitrogen tanks is also very high. It is equipped with double safety automatic control valves, which have double safety measures and are safer to use. It ensures that the liquid nitrogen tank pressure does not exceed the limit, thus avoiding the risk of accidental leakage and explosion. In addition, self-pressurizing liquid nitrogen tanks also have certain sealing properties. Although it looks like a tightly sealed jar from the outside! But it has a vent, a pipe for nitrogen to be discharged. When it is filled with liquid nitrogen, we only need to open the drain valve of the tank, and the nitrogen evaporated by the liquid nitrogen will be discharged to the outside from the drain port. There is no pressure inside the tank. Finally, self-pressurizing liquid nitrogen tanks are easy to operate. It has only 4 valve controls, and users can realize the functions of pressurizing, depressurizing and releasing liquid nitrogen with simple operations. In short, the self-pressurizing liquid nitrogen tank is a highly functional, safe and efficient liquid nitrogen storage device that is widely used in scientific research, medical and agricultural fields.
In laboratories and scientific research institutes, experimental samples and cells often need to be stored in a low-temperature environment for a long time. There are many cryogenic containers on the market, and laboratory liquid nitrogen tanks stand out and are entrusted with reuse. This is because the laboratory liquid nitrogen tank has such advantages. It is made of aviation aluminum, which has strong hardness, light weight, long-term durability and no deformation; large-diameter design facilitates sample pickup and placement; large capacity, stable and safe storage of samples; multi-layer insulation Vacuum design effectively reduces the volatilization of liquid nitrogen; it can meet the different needs of different workers. Although, there are ways to use laboratory liquid nitrogen tanks. 1. Before use It should be placed in a well-ventilated room, away from fire sources, electrical equipment, moisture-proof, and shaded; Avoid storing in places where the temperature is too high or too low, and the humidity is too high or too low; Check the sealing of the liquid nitrogen tank to see if there is air leakage. If there is any abnormality, contact after-sales service in time; 2. When using Use caution when adding liquid nitrogen. New jars should be filled slowly and pre-cooled to prevent the liner from cooling too quickly and reducing its service life; When filling liquid nitrogen, do not pour liquid nitrogen on the vacuum exhaust port to avoid a decrease in vacuum degree; The neck plug is made of thermal insulation material, which can not only prevent the evaporation of liquid nitrogen, but also play a role in fixing the lifting cylinder; Check frequently. If frost is found on the surface of the tank, stop using it; 3.After use Liquid nitrogen tanks should be placed on a stable and flat ground, away from direct sunlight and high temperature environments; When not in use for a long time, it should be cleaned and stored to prevent sample residue from corroding the tank wall and affecting the service life; It is recommended to place it in a well-ventilated area and regularly check the insulation performance of the tank; In short, the role of liquid nitrogen tanks in the laboratory is irreplaceable, but their correct use is crucial. During the operation, you must not only pay attention to the storage of the liquid nitrogen tank, but also the addition and correct use of liquid nitrogen to ensure the safety of staff and samples.
At present, livestock breeding has become an important pillar industry in rural areas. In recent years, with the continuous development and progress of animal husbandry, frozen semen technology has become more and more mature, and artificial insemination and breeding have become the main ways to improve livestock species. This technology can keep semen active for a long time, helping to improve the production capacity and breeding effect of animals during the breeding period. Therefore, it is widely used in the livestock industry. In the application of this technology, a container - livestock liquid nitrogen tank - is indispensable in this case. It is made of high-strength aviation aluminum and is designed for breeding users to carry easily when going out. It is a livestock liquid nitrogen tank specially designed for them. The inner and outer double bladders are multi-layered and insulated, small in size, light and easy to carry, and can reduce the evaporation rate of liquid nitrogen to a great extent. When used for cryopreservation of cattle semen and cell vaccines, it can ensure safe and efficient storage, which is both economical and practical, especially Suitable for animal sperm sampling and breeding work in remote areas. Livestock liquid nitrogen tanks have many advantages, but when using them, you also need to pay attention to the following operating matters to improve work efficiency and reduce operational risks. 1.Regular rehydration When the liquid nitrogen balance inside the livestock liquid nitrogen tank cannot cover the biological samples, liquid nitrogen needs to be added to maintain the low-temperature freezing environment in the tank; 2. Quick access When accessing the frozen semen tube, the time should not exceed 10 seconds. If it is not taken out within the time limit, the bucket needs to be soaked in liquid nitrogen again, and then taken out again after a while; 3. Bucket position When taking out the frozen sperm tube, do not take the bucket outside the liquid nitrogen tank. The bucket should be lower than the mouth of the tank. Use tweezers to pick up the frozen sperm tube after it is exposed; 4. Check the tank During the use of livestock liquid nitrogen tanks, you should check the tank at any time. If any abnormalities are found, such as a dent in the tank, pay attention to the loss of liquid nitrogen. If the loss suddenly increases, the cow essence should be transferred immediately and contact the manufacturer to check the vacuum degree of the tank. If If the vacuum degree is abnormal, it needs to be returned to the factory for repair or replacement. Transfer frozen semen; If you have any needs, please contact KGSQ liquid nitrogen tank:
Liquid nitrogen tanks have a wide range of uses, whether in laboratories or in the livestock industry. As a low-temperature container, it is mainly used to store or transport liquid nitrogen. It is used for low-temperature experiments in laboratories, cryopreservation in animal husbandry, and can also be used for frozen food. However, for larger liquid nitrogen tanks, although it is convenient to store liquid nitrogen, it is not convenient to transport it. So what should we do when transporting liquid nitrogen tanks over short distances? There is a tool called a liquid nitrogen tank trolley, which can easily transfer liquid nitrogen tanks to any location, solving the transportation problem. Not only that, liquid nitrogen tank trolleys have many advantages: 1. Stability and security: The trolley has balance and stability to ensure the safety of the liquid nitrogen tank during transportation. The cart is equipped with baffles to prevent accidental movement or tipping. 2. Portable and easy to operate: Carts are designed to be portable and easy to move between laboratories or other locations. It has a simple operation method and can be controlled by one person. 3. Durability and corrosion resistance: Since liquid nitrogen has a deep low temperature, the trolley is made of stainless steel to ensure low temperature resistance and corrosion resistance. 4.Adjustability: In order to adapt to liquid nitrogen tanks of different sizes and shapes, the trolley can adjust the distance between the four corner baffles to better meet the size requirements. To sum up, the liquid nitrogen tank trolley is an easy-to-use and safe transfer tool, suitable for laboratories, animal husbandry, etc., where there is a demand for liquid nitrogen tank transfer.
As we all know, the storage modes of liquid nitrogen tanks can be divided into two types according to sample storage requirements: gas phase and liquid phase, that is, soaking in liquid nitrogen and storing in nitrogen. In order to make it easier for users to distinguish between the two, regardless of industry or brand, they are accustomed to using storage modes to identify them. Liquid phase storage is liquid phase liquid nitrogen tanks, and gas phase storage is gas phase liquid nitrogen tanks. This is also their main way of distinguishing themselves! Of course, there are other differences besides this, letâ??s introduce them one by one. 1. Model differences: Including capacity size and national standard naming. The liquid phase liquid nitrogen tank has a capacity range of 1-175 liters and is named YDS. The national standard stipulates that the material is aluminum alloy. The gas phase liquid nitrogen tank has a capacity range of 350-1800 liters and is named YDD. The material specified by the national standard is stainless steel. For user use, just select the appropriate model and type based on the number of samples. 2. Mode differences: Liquid phase liquid nitrogen tank, the sample is immersed in liquid nitrogen; The gas phase liquid nitrogen tank meets both liquid phase and gas phase modes. There is a liquid nitrogen storage area at the bottom of the tank to provide liquid nitrogen to the sample area. Note: Vapor phase storage also requires supplementing liquid nitrogen. 3. Management differences: Liquid-phase liquid nitrogen tanks have a wide range of uses and are relatively small in size. Sample classification management relies on buckets and freezing racks. There is no intelligent control and requires manual labor. Regardless of the size of the gas-phase liquid nitrogen tank, it is equipped with an intelligent control system that can display the internal temperature, perform relevant alarms, and automatically replenish liquid nitrogen.
In the animal husbandry industry, some friends who are improving frozen semen usually choose to use liquid nitrogen tanks to store frozen semen. Because when breeding, there will be problems with the distance between the breeding objects. The distance is far. Due to the characteristics of animal semen, if it is not in a low temperature environment, it will quickly lose its activity and the quality of fertilization will not be guaranteed. Use liquid nitrogen tanks to store frozen semen to ensure that the activity of the semen continues to exist. So what kind of liquid nitrogen tank should be used to store frozen semen? Choose a livestock liquid nitrogen tank, which can also be called a liquid nitrogen frozen semen tank. It is an economical liquid nitrogen tank specially designed for livestock breeding users. It is designed to be statically stored for a long time and can be taken out for sampling; it is suitable for users who need to manually transfer and preserve frozen semen, especially those in mountainous areas. In addition, livestock liquid nitrogen tanks are divided into portable and static storage types; Among the many models of livestock liquid nitrogen tanks, the most popular portable model is YDS-16; YDS-16, with a diameter of 50mm, a static storage time of 128 days, can store 6 buckets, and can store nearly 800 frozen semen. The important thing is that the jar is small in size, short in height, has good heat preservation, and can be placed It can be put into the trunk of the car and is convenient for short-distance transportation. Among static storage liquid nitrogen tanks, the most popular models have a capacity of 30 or 35 liters and a diameter of 125mm. The static storage time is about 90 days. It can store 6 buckets and can store nearly 5,000 frozen semen. Important Whatâ??s more, static storage indoors eliminates the need for frequent replenishment of liquid nitrogen, which can meet the needs of a large number of frozen semen customers. Therefore, when improving frozen semen, use livestock liquid nitrogen tanks to store frozen semen, choose portable ones when going out, and choose static storage for long-term storage.
In the medical field, cell storage tanks are often used as cryogenic containers to freeze and preserve biological samples such as cells, vaccines, embryos, etc. During use, the physical characteristics of liquid nitrogen cause it to continuously volatilize. Workers need to observe in time to ensure that the temperature and liquid nitrogen in the storage tank are always in a safe range. At this time, the function of real-time monitoring becomes particularly important. Therefore, the manufacturer designed an intelligent liquid level alarm to quickly solve this problem. In addition, it has many additional functions and is more convenient to use. The functions of the intelligent liquid level alarm are: 1. Temperature display: detect and display the temperature of the cell storage tank in real time. 2. Fault alarm: When the sensor is disconnected or the input is open or short-circuited, the instrument automatically emits an audible and visual alarm, the alarm indicator light on the panel lights up, and the buzzer responds. 3. Over-temperature alarm: When the temperature of the measuring point of the liquid nitrogen container is lower than the low temperature set value or higher than the high temperature set value, the instrument automatically issues an audible and visual alarm. 4. Parameter setting function: directly set the high temperature set value, low temperature set value, control temperature value and control hysteresis value through the setting keys on the panel. 5. Silencing function: When the instrument is in the sound alarm state, if necessary, you can click the "Alarm" button to stop the buzzer and eliminate the sound. Click again to turn on the sound. In the setting state, click the "Alarm" button to save the setting value. 6. Power soft switch: Long press the "Power" button on the panel to turn off the instrument power, and long press the "Power" button again to turn on the instrument power. With the blessing of these functions, the intelligent liquid level alarm adds a layer of guarantee to the safety of biological samples.
Regarding long-term cryopreservation of cells, there are two optional containers, liquid nitrogen tanks and gas-phase liquid nitrogen tanks. The former is to freeze cells in liquid nitrogen; the latter is to freeze cells in a low-temperature environment generated by liquid nitrogen; Both of them also rely on liquid nitrogen for cooling, but some people say that using gas-phase liquid nitrogen tanks is safer. Why is this? In fact, no product can be guaranteed to be perfect. It cannot be said that using gas-phase liquid nitrogen tanks to freeze cells is safer. It can only be said that the safety index is higher than that of conventional liquid nitrogen tanks. Mainly manifested in three aspects: 1. Storage mode security The gas-phase storage mode is used to freeze cells, which uses low-temperature nitrogen volatilized by liquid nitrogen for cryopreservation. There is no contact between cells and liquid nitrogen, which can avoid cross-infection of cells during storage and avoid accidental entry of liquid nitrogen into the cryopreservation tube. pipe burst problem; 2. Temperature safety The temperature inside the gas-phase liquid nitrogen tank is divided into zones. The temperature difference between the upper and lower parts is about 10�°C, which can be said to be very stable. The temperature at the mouth of the tank can be maintained at -190�°C. The lower the temperature, the lower the temperature. No matter where the cells are stored, , the activity will not be "excited" due to large fluctuations in temperature, thereby losing activity. 3. â??Equipmentâ?? security The gas phase liquid nitrogen tank has a complete intelligent monitoring system. It can detect temperature, 3 temperature sensors, transmit internal temperature in real time; It can monitor the liquid level, determine the liquid level height, and can pre-set high/low liquid level alarms; In addition to these functions, there are also heat exhaust functions, lid opening and defogging functions, alarm and remote alarm functions, event recording, and communication functions. These functions all serve the safety of cells. This is why the safety index of gas-phase liquid nitrogen tanks is higher than that of conventional liquid nitrogen tanks.
In laboratories, the most common experimental objects are cells. The safety of cells is related to the accuracy of experimental results. Therefore, they need to be stored in a low-temperature environment. At this time, experimenters usually choose a cell freezing container, using -196�° liquid nitrogen to maintain low temperature. Its vacuum structure can provide a stable low-temperature environment for cells. However, there are also disadvantages. Liquid nitrogen is easily volatile. Although the vacuum structure can effectively reduce it, the surrounding environment will also have an impact. Therefore, when using cell freezing containers, there are requirements for the storage environment. Ambient temperature requirements: A suitable temperature will reduce the volatilization of liquid nitrogen, while an excessively high temperature will accelerate the volatilization. Just like summer and winter, the temperature difference between the two seasons is large, and the volatilization in summer will be much higher than that in winter. The suitable temperature for cell freezing containers is about 20�°, and they can be stored in a cool room or dark place, away from direct sunlight and high temperature environments, to reduce the impact of heat on the tank. Ventilation requirements: It should be placed in a well-ventilated area, away from sources of fire and flammable materials. In order to prevent the discharge of volatile liquid nitrogen, the environment is too sealed and cannot dissipate quickly. It also competes with oxygen for space, causing workers to suffer from hypoxia and suffocation. Storage requirements: The inner and outer bladders of the container are only connected by the neck tube, which is relatively fragile. To prevent the neck tube from rupturing due to stress, we should store it vertically. At the same time, pay attention to avoid collisions during the movement of the jar to prevent the neck tube connection from falling off and causing damage to the vacuum. This is the storage environment requirement of cell freezing containers.
Stem cells are cells with the ability to self-replicate and differentiate into multiple directions. Under certain conditions, they can differentiate into cells with multiple functions and have the ability to repair various tissue functions and regenerate organs. Therefore, they are also called "universal cells." Such important stem cells can only play a greater role if they are preserved intact. Stem cell storage tanks are a common storage method for preserving stem cells. Using liquid nitrogen to place stem cells in a low-temperature environment can effectively extend the storage time and maintain their vitality and function. Since different users have different amounts of stored stem cells, there are three storage tanks to choose from: 1. For liquid phase storage of hundreds or thousands of stem cells, YDS series aviation aluminum liquid nitrogen tanks can be selected; 2. For gas phase storage of thousands of stem cells, small gas-liquid phase stem cell storage tanks can be used, which are also made of aviation aluminum; 3. If the number of stem cells exceeds 10,000, it is more appropriate to use a gas phase stem cell storage tank. Each of these stem cell storage tanks has its own advantages. Liquid phase storage can maintain a constant temperature of -196�°C, but they lack intelligent management. The small gas-liquid phase storage tank is a mini version of the YDD storage tank. It has the same gas-liquid phase dual mode, intelligent management of samples, automatic replenishment and alarm. In addition, the use of aviation aluminum materials with stronger thermal insulation and lighter weight also combines the advantages of YDS. Therefore, when storing stem cells, you must choose an appropriate storage tank based on the number of cells and storage method.
A self-pressurizing liquid nitrogen tank increases the pressure inside the tank through vaporization of liquid nitrogen. When it reaches a certain pressure value, liquid nitrogen can be continuously discharged. Some users of self-pressurizing liquid nitrogen tanks find that the tank cannot discharge liquid nitrogen after using it for a period of time. After self-inspection, they find that the tank is intact. So what is the reason why the tank cannot discharge liquid nitrogen? Reason 1: There is no liquid nitrogen in the tank There is no liquid nitrogen in the tank, so of course it cannot be discharged. The self-pressurizing liquid nitrogen tank itself relies on the vaporization of a small amount of liquid nitrogen to achieve pressurization. Without liquid nitrogen, pressure cannot be generated! Moreover, self-pressurizing liquid nitrogen tanks and liquid nitrogen storage tanks are the same. Even if the tank is filled with liquid nitrogen and left unused, it will naturally evaporate. Therefore, when you find that the self-pressurizing liquid nitrogen tank cannot drain, you must first check whether the liquid nitrogen in the tank has been used up, and replenish it in time. If you only focus on the inability to drain the liquid and do not pay attention to the remaining amount of liquid nitrogen in the tank, this problem will occur a second time. Reason two: ice blockage phenomenon This situation usually occurs at the booster pipe, and sometimes at the drain pipe. This is because the moisture in the air enters the container and is not fully eliminated. When it comes into contact with low temperatures, it will freeze and block the container. Pipes naturally cannot drain fluid.
Simmental cattle are an excellent breed of cattle. Livestock farmers will buy Simmental cattle frozen semen and cross-breed them with local cattle to improve the quality of cattle and gain greater breeding benefits! The purchased frozen beef semen cannot be stored in a normal temperature tank. What kind of tank should be used to store it? â??â??Semen storage tank! After Simmental cattle semen is extracted, it will quickly become inactive at room temperature and cannot be used. However, through the semen storage tank, the semen is specially treated and soaked in liquid nitrogen. The deep low temperature of liquid nitrogen -196�° can be used to preserve sperm for a long time. Activity, wait until use before taking out and thawing. Specific steps for thawing frozen cattle semen for breeding: 1. Lift the bucket to the mouth of the jar and use long tweezers to quickly pinch out the frozen wheat tube; 2. Dissolve the frozen wheat tube in warm water at 30-40�°C, and take it out when it is half melted; 3. Check the sperm activity. If the activity is above 0.3, the breeding success rate is high and it can be used for insemination. 4. Load the semen into the gun and output it as soon as possible. Do not store it for a long time. Notice: Insemination time: The appropriate insemination time is after the cowâ??s estrus period, when the breeding success rate is higher. Storage time of frozen semen at room temperature: After thawing, the longer the frozen semen is stored at room temperature, the lower the activity. Therefore, it should be thawed and infused immediately to avoid long-term storage or repeated freezing.
Frozen embryos are one of the prerequisites for implementation, the other is the cultivation of embryos. After the eggs and sperm are completely extracted, in order to ensure the viability of the eggs and sperm, they will immediately enter the cryopreservation stage, suspend their life activities, and then complete insemination in an external culture system to complete the embryo development. After the embryo is transplanted into the uterus, it still needs to be stored at low temperature. The method of preservation is to put it into a low-temperature medium - liquid nitrogen. Since the temperature of liquid nitrogen is much lower than normal temperature, organisms in direct contact will be frozen. Otherwise, the plastic will be frozen brittle. In order to ensure the vitality of the sperm and egg "combination" embryo, a special liquid nitrogen container is needed to store it. We can also call it an embryo storage tank, because it is used for embryo storage at this time. The embryo storage tank is composed of two layers: an inner and outer bladder. The inner and outer layers are insulated by a vacuum. It has good thermal insulation and can reduce liquid nitrogen consumption. Even so, the liquid nitrogen balance and temperature in the tank need to be tested every day to ensure that the ambient temperature of the cryopreserved embryos is normal and stable. In order to ensure the safety of the embryos and improve work efficiency, we can install a liquid level alarm, which can detect the liquid level in the tank. When the ambient temperature reaches the upper and lower limits, an alarm will sound to remind the staff to replenish liquid nitrogen in time. It also supports text messages. , telephone, and email alarm functions. The addition of a liquid level alarm can avoid embryo loss caused by insufficient liquid nitrogen. This safety measure ensures the safety of the embryonic baby to a certain extent, preventing the embryonic baby from being harmed by the external environment and receiving the same careful care as in the mother's body. It not only improves the success rate of IVF, but also improves the efficiency of laboratory work management. Moreover, using embryo storage tanks to freeze embryos ensures their vitality during the period between the completion of cultivation and delivery to the mother, reducing damage to the embryos due to temperature changes, etc., and ensuring the success rate of IVF.
Liquid nitrogen supply tank is a cryogenic container made of stainless steel, mainly used for liquid nitrogen transfer and replenishment; The container itself has a pressure boosting system, which supplies liquid nitrogen to other containers by controlling valves. It is usually used in conjunction with experimental instruments, electronic equipment and other devices. It is characterized by being equipped with double safety automatic control valves to provide double safety protection measures; it can be continuously pressurized and has the uninterrupted nature of continuous liquid discharge; it is designed with 304 stainless steel inner and outer tanks + vacuum insulation structure, and has been rigorously tested at low temperatures; it is installed with Mobile casters with high load-bearing capacity make movement quick and convenient. Correct use steps of liquid nitrogen supply tank: 1.Add liquid Input liquid from the liquid inlet valve. When filling the tank, you must first open the vent valve, connect the metal hose for infusion to the liquid inlet valve, open the liquid inlet valve, and then add liquid nitrogen to the tank. The filling is completed. Finally, close the liquid inlet valve. 2. Liquid level indication Liquid nitrogen supply tanks are generally equipped with a buoyancy level gauge. By observing the yellow indicator ring on the transparent display, you can grasp changes in the liquid level in the tank. 3.Storage When storing liquid nitrogen, the liquid inlet valve and booster valve should be closed, and the vent valve should be opened; 4.Transportation When the liquid nitrogen supply tank needs to be transported, the opening and closing states of each valve should remain the same as during storage, and the bottom casters should be fixed to prevent losses caused by road bumps. 5.Infusion When you need to add fluid to other containers, pay attention to the following steps: (1) Close the vent valve; (2) Open the booster valve; (3) Observe the pressure gauge; (4) When the pressure rises to 0.05MPa, open the drain valve to replenish liquid nitrogen; Liquid nitrogen supply tank is an important cryogenic equipment, which is widely used and frequently used. Correct use is more conducive to the smooth progress of experimental work.
When there are a large number of stem cell samples that need to be preserved, whether it is the founder of the cell bank, the manager of the hospital blood bank, or the personnel of the preservation center, they all choose a specialized cell storage tank - a gas-phase liquid nitrogen freezer. This liquid nitrogen container Can popular branch! Because it has the advantages of large capacity, small daily evaporation loss of liquid nitrogen, safe and reliable use, and easy operation. In addition to stem cells, it is also suitable for the long-term static storage of various tissues and organs such as plasma, semen, and embryos. Originally made for the storage of plasma and stem cells, it has been widely recognized and favored by blood stations, cord blood banks, sperm banks and other institutions since its launch. What are the advantages of using a gas-phase liquid nitrogen freezer to preserve stem cells? 1. The tank body is made of high-quality stainless steel, with polished surface, good load-bearing, low temperature resistance and corrosion resistance; 2. The tank mouth is designed with an offset structure, large capacity, high vacuum, and low liquid nitrogen loss; 3. Two storage methods, gas phase and liquid phase, can maintain a low temperature environment of -190�° on the top of the jar; 4. Rotating trays are used internally for partitioned storage, making it easier to take out cells; 5. It has an intelligent monitoring system and can modify parameters in the settings according to needs; 6. The system can display high and low liquid levels in real time. Ultra-high and ultra-low will provide liquid level alarms, over-temperature alarms, and automatic control of fluid replenishment; 7. It has a hot gas bypass leakage function in the pipeline. The hot gas in the pipeline will not enter the tank during liquid replenishment, ensuring a low-temperature storage environment to the greatest extent; 8. It has a cover-opening and defogging function, which makes it easy to see the stored items and make them easy to extract;
The stainless steel liquid nitrogen tank is also a self-pressurizing liquid nitrogen tank. The tank itself has a pressurizing system and is adjusted through a pressurizing valve. When the booster valve opens, the pressure increases, and when it closes, the pressure decreases. During use, what is the reason why the booster valve is opened but unable to boost pressure? The reasons why the pressure does not rise after the booster valve of the self-pressurized liquid nitrogen tank is opened are as follows: 1. The booster valve is damaged When the vent valve and the inlet and outlet valve are closed, and the pipeline is unobstructed but does not pressurize, it means that there is a problem with the booster valve itself and the booster valve needs to be replaced. You can contact the manufacturer for replacement. 2. Pipe blockage The main reason for pipeline blockage is that external air and moisture enter and are not discharged in time. Under the influence of low temperature in the tank, they condense into ice and block the pipeline. The correct way to clear the pipe is to use hot nitrogen or hot air to blow away the ice and clear the pipe. After unblocking, it is recommended to transport liquid nitrogen to test whether it is unblocked. Do not use tapping to unblock. 3. Insufficient pressure Check whether the pressure in the liquid nitrogen tank is sufficient. If the pressure in the liquid nitrogen tank is too low, the pressure cannot be increased even if the pressure increase valve is opened. 4. Container leakage The container leaks and the vacuum is destroyed, which will also result in the inability to pressurize. It is easy to judge whether the container is leaking. You can observe the frosting phenomenon on the tank body. The location of a large area of frost is the leakage location. At this time, the jar can no longer be used normally. You can contact the manufacturer for after-sales treatment. In short, there are many reasons why the pressure does not rise after the booster valve of the self-pressurizing liquid nitrogen tank is opened. Check them one by one to find out and solve the problems.
