Description:
Human Keratinocyte growth factor-2 (KGF-2 or FGF-10) is a novel human protein that stimulates the repair of injured mucosal tissues. KGF-2 directly promotes wound-healing by stimulating the proliferation, migration and differentiation of epithelial cells, and has direct chemotactic effects on tissue remodeling. KGF-2 also appears to result in less scarring.
Recombinant Human KGF-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 170 amino acids with molecular weight of about 19.3kD. Recombinant human KGF-2 is highly related to KGF-1(FGF-7) and binds to the same receptor as KGF-1 and shares 57% sequence homology. Recombinant KGF-2 is purified by proprietary chromatographic techniques.
Source : Escherichia Coli.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation : Lyophilized from a concentrated (1mg/ml) solution in water containing 40mM Sodium Phosphate buffer, pH 7.4 , 20 mM NaCl.
Solubility : It is recommended to reconstitute the lyophilized KGF -2 in sterile 18M-cmH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability : Lyophilized KGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 . Upon reconstitution KGF-2 should be stored at 4 between 2-7 days and for future use below -18 . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Amino-Acid Sequence :The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Leu-Gly-Asp.
Dimers and aggregates :Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity :Recombinant human KGF-2 is fully biologically active when compared to standards. The specific activity is about 1.0 x 10 6 Units/mg.
