In the fields of biological sample repository, medical cold storage, animal frozen sperm, vaccine storage and scientific research experiments, the sealing performance of liquid nitrogen tanks is the core to ensure temperature stability and safe storage of samples. Long-term cryopreservation is achieved through a unique vacuum insulation design, the key technologies of which include double chamber design, neck tube design and insulation material filling. Today, we will discuss in depth the hermeticity of liquid nitrogen tanks and how it ensures that the internal liquid nitrogen is isolated from the external environment and maintains its ultra-low temperature status. 1. Vacuuming inside and outside the tank The liquid nitrogen tank has a double-layer design of inner and outer gallbladder, with the inner gallbladder storing liquid nitrogen, and the vacuum sandwich between the outer gallbladder and the inner gallbladder effectively blocking heat conduction, which significantly improves the heat preservation performance and airtightness. 2. Adiabatic material wrapping to maintain low internal temperature Multiple layers of insulating materials are filled to provide additional insulation and sealing protection for the inner liner, reducing the influence of the external environment on the liquid nitrogen inside the tank and improving the safety of sample storage. 3. Insulated neck plugs to isolate heat and guard the safety defense. Do a good job of sealing between the two chambers, insulating and filling between the two chambers, and the important tank opening.