Nowadays, there are still many people who have many doubts about the preparation of serum separation gel. The following will introduce a preparation method of serum separation gel. 1. Select one or more ions from quaternary ammonium salt ion, quaternary phosphonium salt ion, imidazolium salt ion and pyrrole salt ion, and select one or more from halogen ion, tetrafluoroborate ion and hexafluorophosphate ion Multiple ions, prepared into the ionic liquid; 2. Mix the carrier and the silane coupling agent uniformly to obtain a carrier-silane coupling agent blend; 3. Mix the ionic liquid prepared in 1 and the carrier-silane coupling agent blend prepared in 2 uniformly to obtain the serum separating gel. The serum separating gel product prepared by the above method has stable performance, mature and perfect production process, good physiological inertia, thixotropy and sealing, clear and transparent appearance, and all data can meet the requirements.