Rapid Test Card For E. Coli O157

Detection of e. Coli o157 article qualitative detection of colloidal gold feces, food, water and other samples of e. Coli o157. The degree of color is proportional to the sample bacteria. Enrichment effect well. Can be used to treat samples in rapid screening, positive samples can be isolated bacteria, reduce the workload.

Steps:
1. Will be placed on the detection of clean, dry working platform.
2. Provided with the kit with clean straw or from the pipette to take samples or enrichment sample 2 drops, drops added to the test sample by adding end section (detection section white side), start timing;
3. 2-15 minutes of observation, 15 minutes after the results do not count.

[Results judge] negative: a red line appears, which appears in the control line position of a red line; positive: two red lines appear, that in the test line and control line positions are there red line; invalid: no red line appears that the test line and control line in the position not appear red.

[Treatment of samples to be tested]
1. Direct detection: direct detection of water will be.
2. By bacteria detection

(1) stool samples: the samples of the inoculated immediately take 1 gram of 9ml of mec broth, 37 constant temperature shaking culture 6h.
(2) food samples: the 25 g samples of ground, and immediately inoculated in 225ml of mec broth, 37 constant temperature shaking culture 9h.
(3) water samples: water samples will be 400ml immediately inoculated in 5 times concentrated mec 100ml in broth, 37 constant temperature shaking culture 16-20h. 3. Proposed use of enrichment broth was added 20mg / l novobiocin the ec modified broth.

[Interpretation of the results]
1. Enrichment after negative results may be judged as negative.
2. Or by direct detection of bacteria have positive results, the pathogen can be isolated.
3. Negative result does not have a precise meaning. [storage] 4-30 , cool dark dry place.