Barley specifications must comply with ISIRI standards for aflatoxin levels. The following physical factors apply: Test weight minimum of 65 kgs/hl Maximum 3% broken kernels Maximum 0.75% heat damaged kernels Maximum 2% insect kernels Maximum 0.08% mouldy kernels Maximum 2% foreign materials Maximum 2 datura seeds per 100 grams of toxic kernels Maximum 4% other grains Chemistry factors include a maximum moisture content of 14%, a minimum protein level of 10%, and a maximum TVN level of 50 mg/100 grams. Heavy metals in barley must be limited to a maximum of: Lead: 10 mg/kg Cadmium: 1 mg/kg Arsenic: 2 mg/kg Mercury: 0.1 mg/kg Microbiological factors include: E.coli: maximum of 10 in one gram Mould colony: maximum of 5x105 CFU/gram Salmonella: not present in 25 grams Finally, the barley must be free from live insects and have total aflatoxin levels below a maximum of: Total aflatoxin: max.20 Aflatoxin B1: max.5 Aflatoxin B2: max.5